##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364454_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1583818 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.6117243269113 31.0 31.0 34.0 26.0 34.0 2 30.63529206007256 31.0 31.0 34.0 26.0 34.0 3 30.5442563476359 31.0 31.0 34.0 26.0 34.0 4 34.04103628068376 37.0 35.0 37.0 30.0 37.0 5 34.26428604801814 37.0 35.0 37.0 30.0 37.0 6 34.31291411008083 37.0 35.0 37.0 30.0 37.0 7 34.23039957873948 37.0 35.0 37.0 30.0 37.0 8 34.092474640394286 37.0 35.0 37.0 30.0 37.0 9 35.114492321718785 39.0 35.0 39.0 27.0 39.0 10-11 35.37475012911837 39.0 35.0 39.0 29.0 39.0 12-13 35.42742947737682 39.0 35.0 39.0 29.5 39.0 14-15 36.579539126339014 40.0 36.0 41.0 29.0 41.0 16-17 36.7381072193901 40.0 37.0 41.0 30.0 41.0 18-19 36.73819150937797 40.0 36.5 41.0 30.0 41.0 20-21 36.71657160102991 40.0 37.0 41.0 29.0 41.0 22-23 36.656461474740155 40.0 36.0 41.0 29.0 41.0 24-25 36.74088121236152 40.0 36.0 41.0 29.5 41.0 26-27 36.774712119700624 40.0 36.0 41.0 30.0 41.0 28-29 36.73670996288715 40.0 36.0 41.0 30.0 41.0 30-31 36.520137414778716 40.0 36.0 41.0 29.5 41.0 32-33 36.59535944155199 40.0 36.0 41.0 30.0 41.0 34-35 36.54079572274087 40.0 36.0 41.0 30.0 41.0 36-37 36.417472209559435 40.0 36.0 41.0 29.0 41.0 38-39 36.28873361712015 40.0 35.5 41.0 29.0 41.0 40-41 36.25725967251287 40.0 35.0 41.0 29.0 41.0 42-43 36.12468635916501 40.0 35.0 41.0 28.0 41.0 44-45 36.107560969757884 40.0 35.0 41.0 28.0 41.0 46-47 35.96831769811935 39.0 35.0 41.0 28.0 41.0 48-49 35.813878236009444 39.0 35.0 41.0 28.0 41.0 50-51 35.696899201802225 39.0 35.0 41.0 27.5 41.0 52-53 35.57999877511179 39.0 35.0 41.0 28.0 41.0 54-55 35.4556985082882 39.0 34.0 40.5 28.0 41.0 56-57 35.42336493208184 39.0 34.5 40.5 28.0 41.0 58-59 35.41345280834035 39.0 34.0 41.0 28.0 41.0 60-61 35.23393344437302 38.0 34.0 40.5 28.0 41.0 62-63 34.98881121441984 38.0 34.0 40.0 28.0 41.0 64-65 34.7006155378964 37.5 34.0 40.0 27.0 41.0 66-67 34.40212922191817 37.0 34.0 40.0 26.5 41.0 68-69 34.11303508357652 36.5 33.5 39.5 26.0 41.0 70-71 33.77090896807588 36.0 33.0 39.0 26.0 41.0 72-73 33.43204711652476 35.5 33.0 39.0 26.0 40.5 74-75 33.07176645296366 35.0 33.0 37.5 26.0 40.0 76-77 32.7145884186188 35.0 33.0 37.0 26.0 39.0 78-79 32.384401806268144 35.0 32.0 37.0 25.0 39.0 80-81 32.02784915943625 35.0 32.0 36.0 25.0 38.5 82-83 31.721878397644176 35.0 32.0 36.0 24.0 37.0 84-85 31.362788843162534 35.0 32.0 35.5 22.5 37.0 86-87 31.147575668416447 35.0 32.0 35.0 22.5 36.5 88-89 30.994855785197544 35.0 32.0 35.0 21.5 36.0 90-91 30.844899161393542 35.0 31.5 35.0 20.5 36.0 92-93 30.605432568641092 35.0 31.0 35.0 20.0 36.0 94-95 30.51000935713573 35.0 31.0 35.0 19.5 35.0 96-97 30.420830865667646 34.5 31.0 35.0 19.0 35.0 98-99 30.259300311020585 34.0 31.0 35.0 17.5 35.0 100-101 30.12045260250862 34.0 31.0 35.0 16.0 35.0 102-103 29.064431329862394 33.5 29.0 34.5 11.0 35.0 104-105 30.016987747329555 34.0 30.5 35.0 16.0 35.0 106-107 30.25020267480228 35.0 31.0 35.0 16.0 35.0 108-109 30.23075757441827 35.0 31.0 35.0 12.5 35.0 110-111 30.12172579172607 35.0 31.0 35.0 7.5 35.0 112-113 29.99340707076192 35.0 31.0 35.0 3.5 35.0 114-115 29.877675654652236 35.0 31.0 35.0 2.0 35.0 116-117 29.744688152300327 35.0 31.0 35.0 2.0 35.0 118-119 29.47692127504549 35.0 31.0 35.0 2.0 35.0 120-121 29.332753826512892 34.5 31.0 35.0 2.0 35.0 122-123 29.07988701984698 34.0 30.5 35.0 2.0 35.0 124-125 27.86841890924336 33.5 27.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.790750729366156 1101 2 0.7494725255197316 1101 3 0.9700909008901206 1101 4 0.8831862205809529 1101 5 0.742977853852409 1101 6 0.789937324211401 1101 7 0.7822062040487623 1101 8 0.925696278940876 1101 9 1.4478736613500232 1101 10-11 1.128038645533536 1101 12-13 1.0652385767170998 1101 14-15 1.2556129611695255 1101 16-17 1.0336383818935246 1101 18-19 1.164034538161573 1101 20-21 1.0633113999810107 1101 22-23 1.0436889721152198 1101 24-25 1.1457417703646158 1101 26-27 1.0072471610387623 1101 28-29 0.934252587817376 1101 30-31 1.0776481644541036 1101 32-33 1.0581324080983947 1101 34-35 0.9462104639209059 1101 36-37 0.9743910032774181 1101 38-39 0.9790195223286418 1101 40-41 0.9566476770424543 1101 42-43 1.0084057023132331 1101 44-45 0.8508438582406868 1101 46-47 0.872864437156494 1101 48-49 0.8709258013638603 1101 50-51 0.8454444813574966 1101 52-53 0.6473906847986655 1101 54-55 0.8107585576831937 1101 56-57 0.8260463560973861 1101 58-59 0.8023096435236852 1101 60-61 0.7313276925367518 1101 62-63 0.8338321990778752 1101 64-65 0.8027397415979323 1101 66-67 0.7852337326641106 1101 68-69 0.7260517812684597 1101 70-71 0.720758285471355 1101 72-73 0.6913394699098134 1101 74-75 0.6771272308509566 1101 76-77 0.7200228810773694 1101 78-79 0.5287406448924088 1101 80-81 0.6432576258860507 1101 82-83 0.6283878558956779 1101 84-85 0.7704681201547281 1101 86-87 0.7701035161416812 1101 88-89 0.6906557971312992 1101 90-91 0.6827948036656224 1101 92-93 0.8559650493591846 1101 94-95 0.6549868686919567 1101 96-97 0.540951201560862 1101 98-99 0.6762343510268032 1101 100-101 0.7051205466962642 1101 102-103 0.6934809629305256 1101 104-105 0.6572075641318555 1101 106-107 0.5254493901253952 1101 108-109 0.5597180413752092 1101 110-111 0.6772296021560038 1101 112-113 0.5799483591053018 1101 114-115 0.6773683452493913 1101 116-117 0.681466070420317 1101 118-119 0.5573572930917869 1101 120-121 0.5473605924991105 1101 122-123 0.6509051330874804 1101 124-125 0.33598739545250567 1102 1 0.29554382380725386 1102 2 0.28995048111031707 1102 3 0.35523656093397804 1102 4 0.26635243604699355 1102 5 0.270393891027517 1102 6 0.08554545357335996 1102 7 0.2997043833925801 1102 8 0.2850225933741086 1102 9 0.7702991629175244 1102 10-11 0.4282249639629043 1102 12-13 0.5085604299764839 1102 14-15 0.5750172052235101 1102 16-17 0.40158934723135076 1102 18-19 0.2854716763266083 1102 20-21 0.5286087117513389 1102 22-23 0.4197321061905086 1102 24-25 0.3353167459172397 1102 26-27 0.3011851183103431 1102 28-29 0.3197004742708387 1102 30-31 0.42459426850152227 1102 32-33 0.3003337839960807 1102 34-35 0.20398820143537932 1102 36-37 0.22508793586808906 1102 38-39 0.37461376085146014 1102 40-41 0.33898899811158856 1102 42-43 0.36816761534350206 1102 44-45 0.25354406625096004 1102 46-47 0.27965162195145865 1102 48-49 0.2259263273032417 1102 50-51 0.1731912815574077 1102 52-53 0.11173023327593512 1102 54-55 0.012132190555355749 1102 56-57 0.12225617017655566 1102 58-59 0.21087118170260766 1102 60-61 0.12136760856763829 1102 62-63 0.17123363577314876 1102 64-65 0.16963505349004748 1102 66-67 0.07643177224394293 1102 68-69 0.20112577766791162 1102 70-71 0.27470632870004863 1102 72-73 0.25077892422128656 1102 74-75 0.28010213523820227 1102 76-77 0.2327430647182851 1102 78-79 0.07880906734101245 1102 80-81 0.15388336289321103 1102 82-83 0.15987098985017312 1102 84-85 0.3091727289439028 1102 86-87 0.3109214449873683 1102 88-89 0.224138004023267 1102 90-91 0.2501233486857828 1102 92-93 0.2852876042066086 1102 94-95 0.31005795606785114 1102 96-97 0.2577944982998872 1102 98-99 0.2665044221569559 1102 100-101 0.15424434175404755 1102 102-103 0.07692172378613904 1102 104-105 0.10908687891085478 1102 106-107 0.07102744783377801 1102 108-109 0.21036368858513654 1102 110-111 0.02689977022006218 1102 112-113 0.022899035050649275 1102 114-115 0.009513156240871012 1102 116-117 -0.11075843171137478 1102 118-119 0.12724658411040224 1102 120-121 0.010063437755661653 1102 122-123 -0.05353404693024544 1102 124-125 -0.4502179946963736 1103 1 0.6153528797679684 1103 2 0.5505282532346811 1103 3 0.6951567939622976 1103 4 0.6136539070113329 1103 5 0.4368029572182195 1103 6 0.40686612460661564 1103 7 0.4571763451539681 1103 8 0.5763756301993723 1103 9 1.0691534044582696 1103 10-11 0.8221305842635047 1103 12-13 0.7028310656086063 1103 14-15 0.9306897662467293 1103 16-17 0.7452303171148031 1103 18-19 0.7651119846260883 1103 20-21 0.7538014998802467 1103 22-23 0.845931336518646 1103 24-25 0.6595027385085785 1103 26-27 0.7524397386297892 1103 28-29 0.6541665934986298 1103 30-31 0.7621541172861725 1103 32-33 0.7591306635810682 1103 34-35 0.7167457346088071 1103 36-37 0.7548764611886583 1103 38-39 0.8191793498698416 1103 40-41 0.8193013222836925 1103 42-43 0.7437544935016689 1103 44-45 0.5915456720895946 1103 46-47 0.6671307749893671 1103 48-49 0.6009185875110177 1103 50-51 0.554059474241889 1103 52-53 0.44192701581065563 1103 54-55 0.5498333073737243 1103 56-57 0.521904127409833 1103 58-59 0.581551536360081 1103 60-61 0.5847578865809737 1103 62-63 0.6188697349717103 1103 64-65 0.6529063338805088 1103 66-67 0.5488572481402088 1103 68-69 0.5948379031629187 1103 70-71 0.7319606340435953 1103 72-73 0.6609786579724286 1103 74-75 0.6422870583921565 1103 76-77 0.6769210752282504 1103 78-79 0.5762109368709929 1103 80-81 0.5595503546311491 1103 82-83 0.5179337164914806 1103 84-85 0.6046262572123204 1103 86-87 0.5427984691112897 1103 88-89 0.5670712876873267 1103 90-91 0.5113682572485523 1103 92-93 0.5973286008764802 1103 94-95 0.5552650897333891 1103 96-97 0.4999933766357394 1103 98-99 0.5376807460652415 1103 100-101 0.3802292807914007 1103 102-103 0.3595722055377273 1103 104-105 0.3259869844412364 1103 106-107 0.34910059963869955 1103 108-109 0.30517008609548313 1103 110-111 0.2664555936397264 1103 112-113 0.3266340568701338 1103 114-115 0.3799453327781279 1103 116-117 0.24189182457670455 1103 118-119 0.157522043315641 1103 120-121 -0.033717622367042566 1103 122-123 0.267792092434469 1103 124-125 -0.056234976473540144 1104 1 0.2889945122836579 1104 2 0.44268204858901683 1104 3 0.526410665113417 1104 4 0.27785417314855465 1104 5 0.295107336462344 1104 6 0.3103544383261152 1104 7 0.45364794115710794 1104 8 0.5175903443917491 1104 9 0.9563533637316368 1104 10-11 0.6015124445089413 1104 12-13 0.506474437490823 1104 14-15 0.6747566526675044 1104 16-17 0.5129793682408703 1104 18-19 0.5854983502550724 1104 20-21 0.59469489085307 1104 22-23 0.6368094550048653 1104 24-25 0.6721706828318759 1104 26-27 0.6070126110168488 1104 28-29 0.6355920503298194 1104 30-31 0.6925731808847644 1104 32-33 0.6533847793747967 1104 34-35 0.5458257511257187 1104 36-37 0.476870804005074 1104 38-39 0.5757288426253098 1104 40-41 0.4703550531542007 1104 42-43 0.4471985099393905 1104 44-45 0.3776331084895901 1104 46-47 0.37265717023000633 1104 48-49 0.5006561270002976 1104 50-51 0.43451717864574135 1104 52-53 0.24254708937313296 1104 54-55 0.2458723773641367 1104 56-57 0.23141922242449198 1104 58-59 0.30353341725164995 1104 60-61 0.4724568531427167 1104 62-63 0.5051361988536911 1104 64-65 0.4977520110597382 1104 66-67 0.3508897615672737 1104 68-69 0.31830089707938214 1104 70-71 0.4883650028232438 1104 72-73 0.42854113375563685 1104 74-75 0.3617000151172789 1104 76-77 0.44225003822641185 1104 78-79 0.4295620987112727 1104 80-81 0.48932069107836895 1104 82-83 0.3287184783679571 1104 84-85 0.38589729223439306 1104 86-87 0.5404295544194397 1104 88-89 0.4896589595381542 1104 90-91 0.41428261536573885 1104 92-93 0.4195008222030907 1104 94-95 0.4264779903179843 1104 96-97 0.41732335355731465 1104 98-99 0.410620462873748 1104 100-101 0.4993993979087499 1104 102-103 0.4296983732465982 1104 104-105 0.3999480664686281 1104 106-107 0.4061321125764792 1104 108-109 0.25822133094455424 1104 110-111 0.371369975796938 1104 112-113 0.3947564177703029 1104 114-115 0.412994357460164 1104 116-117 0.478708257065243 1104 118-119 0.3277967465627398 1104 120-121 0.22623432940018162 1104 122-123 0.2584756104517645 1104 124-125 0.05836735908561863 1105 1 0.5307327876887307 1105 2 0.5807545876811773 1105 3 0.6233684897549665 1105 4 0.5073838457332442 1105 5 0.4003650566681216 1105 6 0.47710559007149556 1105 7 0.4731436364896595 1105 8 0.6068890368070257 1105 9 1.1182109972129695 1105 10-11 0.7603418477592712 1105 12-13 0.7382368573063403 1105 14-15 0.48582180593158597 1105 16-17 0.5722011181609616 1105 18-19 0.5746865504378889 1105 20-21 0.8117907518985348 1105 22-23 0.8576837330645972 1105 24-25 0.7688167778908266 1105 26-27 0.8657049041454599 1105 28-29 0.7796877139041314 1105 30-31 0.8647033856236561 1105 32-33 0.6895031684173887 1105 34-35 0.6751087883541089 1105 36-37 0.7692499954319771 1105 38-39 0.8913432120103622 1105 40-41 0.7435602032831667 1105 42-43 0.7528759581235747 1105 44-45 0.7112372012318104 1105 46-47 0.799947581071784 1105 48-49 0.7615020262167178 1105 50-51 0.692267157778943 1105 52-53 0.6748671616027835 1105 54-55 0.6408861799866301 1105 56-57 0.6058072145500759 1105 58-59 0.7351783722709868 1105 60-61 0.7458080708475308 1105 62-63 0.7790391143014403 1105 64-65 0.7073500758424913 1105 66-67 0.6203213296714054 1105 68-69 0.6517011568455544 1105 70-71 0.5938492041135248 1105 72-73 0.5571294593428107 1105 74-75 0.621254463205787 1105 76-77 0.642882760213503 1105 78-79 0.5305100620101442 1105 80-81 0.5826069774196725 1105 82-83 0.5613305839600535 1105 84-85 0.7412601098423046 1105 86-87 0.65949600447858 1105 88-89 0.5717904162848484 1105 90-91 0.516232948318379 1105 92-93 0.4639305581006994 1105 94-95 0.40145081258784643 1105 96-97 0.44630784291117465 1105 98-99 0.4842626960484928 1105 100-101 0.325421463782682 1105 102-103 0.27296557410326017 1105 104-105 0.30547517192840346 1105 106-107 0.20793978789319567 1105 108-109 0.32414386728840583 1105 110-111 0.34372528746544617 1105 112-113 0.36378322091599813 1105 114-115 0.33723439890874474 1105 116-117 0.341454866974221 1105 118-119 0.23634699279094207 1105 120-121 0.22288469960768964 1105 122-123 0.2990393905788409 1105 124-125 0.1672449595465224 1106 1 0.4965509241309647 1106 2 0.4093208805904851 1106 3 0.5509062071611268 1106 4 0.3337292831006664 1106 5 0.3327515182138967 1106 6 0.200679637406175 1106 7 0.280409027984156 1106 8 0.44742418128890904 1106 9 0.959015021195242 1106 10-11 0.42837668028150944 1106 12-13 0.5110604188752959 1106 14-15 0.730257782900047 1106 16-17 0.5556679999312308 1106 18-19 0.4906617065662573 1106 20-21 0.6313332408016805 1106 22-23 0.5782934210455366 1106 24-25 0.509111119240039 1106 26-27 0.5488697792724793 1106 28-29 0.5015987192460756 1106 30-31 0.5723541215513137 1106 32-33 0.5904856927353848 1106 34-35 0.45801904499290913 1106 36-37 0.4963840964831263 1106 38-39 0.6121437895813528 1106 40-41 0.5586401837931163 1106 42-43 0.5281484028770009 1106 44-45 0.3049825589752473 1106 46-47 0.4592297173714073 1106 48-49 0.3192925497741328 1106 50-51 0.3608939708586618 1106 52-53 0.3876600322851118 1106 54-55 0.20030335024529222 1106 56-57 0.1445377467829374 1106 58-59 0.3005695696612136 1106 60-61 0.27826004391798165 1106 62-63 0.3671060638115051 1106 64-65 0.3381896632238721 1106 66-67 0.17734606658544294 1106 68-69 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-1.1833206839241548 2216 88-89 -1.5545648735396114 2216 90-91 -1.295492436348816 2216 92-93 -1.031278781673855 2216 94-95 -1.063884596387009 2216 96-97 -1.0423575624512047 2216 98-99 -0.9645848330809024 2216 100-101 -1.030797654161514 2216 102-103 -1.1911091428686298 2216 104-105 -0.9750283395182429 2216 106-107 -0.8481894774276739 2216 108-109 -0.9065059799468287 2216 110-111 -0.8435378353035432 2216 112-113 -1.0759229135537325 2216 114-115 -0.9551957854498596 2216 116-117 -0.8370914798354718 2216 118-119 -0.677812219322476 2216 120-121 -0.4862938912827559 2216 122-123 -0.5329831965644303 2216 124-125 -0.4435675102219747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25649.0 3 4967.0 4 3589.0 5 3116.0 6 3492.0 7 4039.0 8 4195.0 9 4434.0 10 4947.0 11 5453.0 12 5873.0 13 6352.0 14 6649.0 15 6451.0 16 6313.0 17 5868.0 18 5431.0 19 5193.0 20 4925.0 21 5137.0 22 6169.0 23 8221.0 24 12603.0 25 18242.0 26 21549.0 27 21399.0 28 24432.0 29 29979.0 30 35736.0 31 43674.0 32 55002.0 33 72359.0 34 98485.0 35 144663.0 36 253389.0 37 432989.0 38 182337.0 39 517.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.05344366265718 19.075033461534087 12.307082722978942 23.56444015282979 2 15.244656253827785 21.180517409241236 39.74115647418194 23.833669862749034 3 18.515637529059525 27.03738687147134 29.309807061164854 25.137168538304277 4 11.976648301344502 16.891908103514247 37.65322500192577 33.47821859321548 5 12.781285515847959 40.41652986488193 33.83167066548807 12.970513953782042 6 30.66856335403021 37.45124111795956 18.367371421139097 13.512824106871136 7 28.244298425891373 34.12079804674404 20.89786888257924 16.73703464478535 8 26.438565475438818 36.02746558908953 20.225533526960472 17.308435408511176 9 27.414921875049323 16.649482355511303 19.533857933627097 36.40173783581227 10-11 24.26722284419641 28.206245378605477 27.637817798817554 19.888713978380558 12-13 24.65596743000488 25.827114596704487 27.716002573575143 21.800915399715493 14-15 22.216907819527936 26.55806974159059 25.36602290833792 25.858999530543556 16-17 22.2143893540904 30.047001418124463 25.89990289067561 21.838706337109524 18-19 22.288772606388438 28.798105324244734 27.430016061551072 21.48310600781576 20-21 23.463772879650882 27.88061234058959 26.98640790310007 21.669206876659462 22-23 23.14068243226604 28.0976523355091 26.640299458406137 22.12136577381873 24-25 22.630475065284497 28.057099900198622 27.122326303456173 22.190098731060708 26-27 22.558547408601317 28.436288895448968 26.76360502067215 22.241558675277563 28-29 22.404693341281014 28.200522766285456 27.0682623909154 22.326521501518133 30-31 22.444409571439895 27.908426998399456 27.228361309446942 22.418802120713714 32-33 22.34955464170867 28.140064819567854 26.961398072081938 22.548982466641537 34-35 22.605993563577194 28.063030725489785 26.82420559679606 22.50677011413696 36-37 22.826853204958823 27.691692814165393 26.775101835549677 22.706352145326107 38-39 22.437181662229065 27.87361891839504 27.081195822991727 22.60800359638417 40-41 22.646397992663804 27.716733999856675 27.132186990640495 22.504681016839033 42-43 22.52761042988073 27.62968764847818 27.091732956007363 22.750968965633724 44-45 22.33511848688348 27.91238688625613 27.035384428881354 22.717110197979043 46-47 22.436531460954736 28.002145477654484 26.759309040018714 22.802014021372063 48-49 22.329388401363282 27.744821783475487 27.065327378838177 22.86046243632305 50-51 22.53377191279303 27.576393867843368 27.039092987874664 22.850741231488943 52-53 22.73828981752501 27.728892499288847 26.819374798138405 22.713442885047737 54-55 22.250230080840065 27.734694296363067 27.06814655083958 22.94692907195729 56-57 22.228477089872133 27.93906250443989 27.129985025152248 22.702475380535727 58-59 22.470707540140584 27.365370869441126 27.067521045865213 23.09640054455308 60-61 22.37449712657287 27.55860042218951 27.263680272585656 22.80322217865196 62-63 22.23316772990697 27.680494315966286 27.201807271711175 22.88453068241557 64-65 22.119932208441362 27.531417371460616 27.212728850182927 23.135921569915098 66-67 22.122933179758423 27.855722938736744 26.903268013478222 23.118075868026615 68-69 22.048087627690926 27.578524749480458 27.208673353081476 23.16471426974714 70-71 22.048679185001234 27.544092938500036 27.24976116916926 23.157466707329473 72-73 22.134563440004673 27.445523734649317 27.127483064721105 23.292429760624906 74-75 21.84106450384596 27.600307146136267 27.179275718595036 23.37935263142274 76-77 22.11442837902601 27.42368821007076 27.176866302590145 23.28501710831308 78-79 22.24150913611429 27.431927068945107 27.227435968270253 23.099127826670347 80-81 22.014582656470868 27.801358113682582 27.090695939330388 23.09336329051616 82-83 22.087860770129193 27.553559604923024 27.176903386369585 23.1816762385782 84-85 22.09785436236292 27.19583154548615 27.269871309733436 23.436442782417497 86-87 21.97004165599718 27.00870282915358 27.482349167116865 23.53890634773238 88-89 22.134922990458268 27.050714524776296 27.497079383923666 23.317283100841767 90-91 22.086612072495722 27.291033954071814 27.360336999080275 23.26201697435219 92-93 22.14613299386803 26.86524376652415 27.455614090588487 23.533009149019332 94-95 21.941904327719048 26.865854474779916 27.463632586515864 23.728608610985173 96-97 22.053307208353765 26.85601383369447 27.480765399598845 23.609913558352925 98-99 22.19276273126735 26.893154656855163 27.201447984143652 23.712634627733834 100-101 21.734966949642722 27.003322716469107 27.60295243122823 23.65875790265994 102-103 21.88632588490888 26.793805472070854 27.52491359215116 23.7949550508691 104-105 21.80671470310212 26.84612028220722 27.639157167041645 23.70800784764902 106-107 21.704260920655376 26.896653186890067 27.57359916980825 23.825486722646307 108-109 21.89081082104984 26.994945588518497 27.142379077704078 23.971864512727585 110-111 21.708323809355427 27.203827439411715 27.41330233462691 23.674546416605946 112-113 21.64617792355689 27.281174256452324 27.44642286025317 23.626224959737623 114-115 21.53863689321124 27.18948499087208 27.314579474779983 23.9572986411367 116-117 21.316329868315865 27.31258658860169 27.41889244890562 23.952191094176825 118-119 21.409608840981605 27.370216104798413 27.16032821479814 24.059846839421844 120-121 21.508571118208362 27.29261544310689 27.33631889372167 23.862494544963084 122-123 21.561781793401547 27.343054786275612 27.27494061720216 23.820222803120682 124-125 21.486754395819126 27.46380262681145 27.2048871793817 23.844555797987724 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2053.0 1 1534.5 2 854.5 3 592.0 4 432.5 5 336.5 6 311.5 7 310.5 8 317.5 9 320.0 10 295.5 11 279.5 12 258.0 13 270.5 14 340.0 15 380.5 16 406.0 17 510.0 18 697.0 19 884.0 20 1055.0 21 1369.5 22 1917.0 23 2785.0 24 3943.5 25 5368.0 26 7341.5 27 9636.0 28 11597.0 29 14767.0 30 19214.0 31 22095.5 32 24922.0 33 28550.0 34 32597.0 35 35452.0 36 38901.5 37 43069.5 38 45421.0 39 47405.0 40 49471.5 41 53324.0 42 56592.0 43 59511.0 44 62482.5 45 63285.5 46 62331.0 47 61009.5 48 60722.0 49 59590.5 50 58105.0 51 56339.5 52 54726.0 53 53464.0 54 51125.5 55 47959.5 56 42759.0 57 37310.5 58 34221.5 59 31590.0 60 26933.5 61 21546.0 62 17004.0 63 13158.5 64 10425.0 65 8416.5 66 6239.5 67 4729.5 68 3378.5 69 2084.5 70 1516.5 71 1137.5 72 886.5 73 729.5 74 645.5 75 520.0 76 318.5 77 161.0 78 85.5 79 56.5 80 42.0 81 34.5 82 22.0 83 10.0 84 7.0 85 8.0 86 6.5 87 5.0 88 2.5 89 2.5 90 2.0 91 3.0 92 4.5 93 3.0 94 1.5 95 1.0 96 1.5 97 1.5 98 1.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08864654903530582 2 2.525542707558571E-4 3 0.0 4 0.0022729884368027134 5 0.0011364942184013567 6 2.525542707558571E-4 7 6.313856768896427E-5 8 0.0011364942184013567 9 5.682471092006784E-4 10-11 0.009533923721033604 12-13 0.0018310184629799638 14-15 0.004514407589760945 16-17 0.0022729884368027134 18-19 0.014048331310794547 20-21 0.018152338210577226 22-23 0.010039032262545317 24-25 0.010891402926346334 26-27 0.010449432952523586 28-29 0.008050167380342943 30-31 0.018594308184399975 32-33 0.0023361270044916773 34-35 0.001199632786090321 36-37 9.155092314899819E-4 38-39 3.1569283844482134E-4 40-41 0.0017363106114465171 42-43 0.002620250559092017 44-45 8.839399476454998E-4 46-47 0.0013574792053127316 48-49 0.008870968760299478 50-51 0.004324991886694052 52-53 0.007671335974209158 54-55 0.008555275921854658 56-57 0.010260017249456693 58-59 0.00846056807032121 60-61 0.01067041793943496 62-63 0.013606361336971799 64-65 0.008839399476454997 66-67 0.014269316297705923 68-69 0.013195960646993531 70-71 0.0038514526290268198 72-73 0.013606361336971799 74-75 0.013069683511615603 76-77 0.005366778253561962 78-79 0.008239583083409836 80-81 0.014174608446172477 82-83 0.01758409110137655 84-85 0.013227529930838014 86-87 0.015942488341463477 88-89 0.015658364786863138 90-91 0.012217312847814585 92-93 0.009533923721033604 94-95 0.007576628122675711 96-97 0.009376077301811194 98-99 0.006250718201207462 100-101 0.010986110777879782 102-103 0.012280451415503548 104-105 0.010039032262545317 106-107 0.005524624672784373 108-109 0.0033463440875151056 110-111 0.00606130249814057 112-113 0.00906038446336637 114-115 0.013574792053127317 116-117 0.011364942184013566 118-119 0.21785962781077117 120-121 0.02645505986167603 122-123 0.028222939756967026 124-125 0.2377482766327949 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1583818.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.01862322555253 #Duplication Level Percentage of deduplicated Percentage of total 1 82.4490303337055 53.607224385793494 2 9.544795508688876 12.411789258887762 3 3.365304527527366 6.564224013436435 4 1.5202674093602229 3.9538277556511665 5 0.8677180645678166 2.820891695307027 6 0.5366371569904029 2.0934845471514416 7 0.3468361730504088 1.5785567319589948 8 0.23020332907443802 1.1974002814687015 9 0.17830076334546754 1.0433583137508629 >10 0.890240789307788 10.32223182851876 >50 0.05035762859848741 2.2576928609957454 >100 0.020308315783210736 2.1493183270796523 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.5784641922241067E-4 0.0 22-23 0.0 3.1569283844482134E-5 0.0 3.788314061337856E-4 0.0 24-25 0.0 6.313856768896427E-5 0.0 0.0010733556507123926 0.0 26-27 0.0 6.313856768896427E-5 0.0 0.0023992655721806417 0.0 28-29 0.0 6.313856768896427E-5 0.0 0.006376995336585391 0.0 30-31 0.0 6.313856768896427E-5 0.0 0.019667663835112367 0.0 32-33 0.0 6.313856768896427E-5 0.0 0.04896395924279179 0.0 34-35 0.0 6.313856768896427E-5 0.0 0.09303467948968884 0.0 36-37 0.0 6.313856768896427E-5 0.0 0.16195042612219335 0.0 38-39 0.0 6.313856768896427E-5 0.0 0.2671077106081633 0.0 40-41 0.0 6.313856768896427E-5 0.0 0.38085184029983243 0.0 42-43 0.0 6.313856768896427E-5 0.0 0.4934594757731002 0.0 44-45 0.0 6.313856768896427E-5 0.0 0.621125659640186 0.0 46-47 0.0 6.313856768896427E-5 0.0 0.7765728132904159 0.0 48-49 0.0 6.313856768896427E-5 0.0 0.9473626388890644 0.0 50-51 0.0 6.313856768896427E-5 0.0 1.1336845521391976 0.0 52-53 0.0 6.313856768896427E-5 0.0 1.3137873164719684 0.0 54-55 0.0 9.47078515334464E-5 0.0 1.5004564918443912 0.0 56-57 0.0 1.2627713537792854E-4 0.0 1.718600243209763 0.0 58-59 0.0 1.2627713537792854E-4 0.0 1.9679660162973271 0.0 60-61 0.0 1.2627713537792854E-4 0.0 2.251237200233865 0.0 62-63 0.0 1.2627713537792854E-4 0.0 2.5341295527642695 0.0 64-65 0.0 1.2627713537792854E-4 0.0 2.80537283955606 0.0 66-67 0.0 1.2627713537792854E-4 0.0 3.0981463779297873 0.0 68-69 0.0 1.2627713537792854E-4 0.0 3.4502386006472965 0.0 70-71 0.0 1.2627713537792854E-4 0.0 3.8447283715679452 0.0 72-73 0.0 1.2627713537792854E-4 0.0 4.2383657718247925 0.0 74-75 0.0 1.2627713537792854E-4 0.0 4.618586226447736 0.0 76-77 0.0 1.2627713537792854E-4 0.0 5.019863393394948 0.0 78-79 0.0 1.2627713537792854E-4 0.0 5.463411831409922 0.0 80-81 0.0 1.2627713537792854E-4 0.0 5.9821898728262966 0.0 82-83 0.0 1.2627713537792854E-4 0.0 6.506239984644701 0.0 84-85 0.0 1.2627713537792854E-4 0.0 7.016873150829198 0.0 86-87 0.0 1.2627713537792854E-4 0.0 7.533315065241082 0.0 88-89 0.0 1.2627713537792854E-4 0.0 8.099573309559558 0.0 90-91 0.0 1.2627713537792854E-4 0.0 8.723224511907302 0.0 92-93 0.0 1.2627713537792854E-4 0.0 9.384190607759225 0.0 94-95 0.0 1.5784641922241064E-4 0.0 10.044651595069634 0.0 96-97 0.0 1.8941570306689278E-4 0.0 10.688664985497072 0.0 98-99 0.0 1.8941570306689278E-4 0.0 11.347105538641435 0.0 100-101 0.0 1.8941570306689278E-4 0.0 12.087847214768363 0.0 102-103 0.0 1.8941570306689278E-4 0.0 12.892548259964213 0.0 104-105 0.0 1.8941570306689278E-4 0.0 13.681938202495488 0.0 106-107 0.0 1.8941570306689278E-4 0.0 14.440074554020727 0.0 108-109 0.0 1.8941570306689278E-4 0.0 15.199599954035122 0.0 110-111 0.0 1.8941570306689278E-4 0.0 16.014813570751183 0.0 112-113 0.0 1.8941570306689278E-4 0.0 16.90506737516558 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1905 0.0 61.563206 1 GTATCAA 3400 0.0 55.67987 1 TCAACGC 3925 0.0 46.368195 4 ATCAACG 3930 0.0 46.005077 3 CAACGCA 4105 0.0 44.195698 5 TATCAAC 4510 0.0 42.066742 2 AACGCAG 4360 0.0 41.337997 6 GTACATG 11515 0.0 41.101074 1 TACATGG 11610 0.0 39.85402 2 ACATGGG 11995 0.0 36.641136 3 ACGCAGA 5345 0.0 33.72005 7 CATGGGA 8870 0.0 33.25803 4 CGCAGAG 5470 0.0 33.058228 8 CATGGGG 3335 0.0 31.209057 4 ATGGGAG 3520 0.0 30.248487 5 GCAGAGT 6060 0.0 29.152588 9 GTATAGG 505 0.0 28.292404 1 GAGTACA 8065 0.0 26.86872 1 AGTACAT 8020 0.0 25.80653 2 ATGGGAT 3475 0.0 25.16262 5 >>END_MODULE