FastQCFastQC Report
Thu 26 May 2016
SRR1364454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364454_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583818
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45240.2856388802248743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39230.2476926010438068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24500.15468949083796243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30750.078.459571
GTATCAA62550.060.4759141
ATCAACG81750.046.1004263
TCAACGC82750.045.543324
TATCAAC83850.045.0892872
CAACGCA84300.044.680525
AACGCAG88600.042.6423346
GTATAGA4200.038.295741
GTACATG112050.036.4178851
ACGCAGA105700.035.5748447
CGCAGAG106350.035.35638
TACATGG115400.034.9805452
ACATGGG118500.033.51173
GCAGAGT118650.031.4403279
CATGGGG32850.030.448294
TGGTATC11950.030.3923742
CATGGGA91050.030.079244
TATCACG800.005345586729.7695792
GAGTACT62350.028.43590212-13
ATGGGAG33100.026.785545
Produced by FastQC (version 0.11.5)