##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364454_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1583818 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.939607960005507 33.0 31.0 34.0 30.0 34.0 2 31.856757531484046 33.0 31.0 34.0 30.0 34.0 3 32.06914051993348 33.0 31.0 34.0 30.0 34.0 4 35.767300283239614 37.0 35.0 37.0 35.0 37.0 5 35.72014524396111 37.0 35.0 37.0 33.0 37.0 6 35.79310185892571 37.0 35.0 37.0 33.0 37.0 7 35.70388958832391 37.0 35.0 37.0 33.0 37.0 8 35.72090353815906 37.0 35.0 37.0 33.0 37.0 9 37.348017259558866 39.0 37.0 39.0 34.0 39.0 10-11 37.30846694506566 39.0 37.0 39.0 33.5 39.0 12-13 37.28232536819256 39.0 37.0 39.0 33.0 39.0 14-15 38.559607858983796 40.0 38.0 41.0 34.0 41.0 16-17 38.581817165861224 40.0 38.0 41.0 34.0 41.0 18-19 38.66171776050026 40.0 38.0 41.0 34.0 41.0 20-21 38.67262400099001 40.0 38.0 41.0 34.0 41.0 22-23 38.637038157161996 40.0 38.0 41.0 34.0 41.0 24-25 38.58506406670463 40.0 38.0 41.0 34.0 41.0 26-27 38.46461241127453 40.0 38.0 41.0 34.0 41.0 28-29 38.385936073462986 40.0 38.0 41.0 34.0 41.0 30-31 38.238277062137186 40.0 38.0 41.0 33.5 41.0 32-33 38.08398408150431 40.0 37.0 41.0 33.0 41.0 34-35 37.96535397375203 40.0 37.0 41.0 33.0 41.0 36-37 37.852263959621624 40.0 37.0 41.0 33.0 41.0 38-39 37.74859106286202 40.0 37.0 41.0 33.0 41.0 40-41 37.61131297914281 40.0 37.0 41.0 33.0 41.0 42-43 37.44343068458623 40.0 37.0 41.0 32.0 41.0 44-45 37.228012625188 40.0 36.0 41.0 31.0 41.0 46-47 36.9328736003758 40.0 36.0 41.0 31.0 41.0 48-49 36.6599353587344 40.0 36.0 41.0 30.0 41.0 50-51 36.44009033866265 39.5 35.5 41.0 30.0 41.0 52-53 36.32448519968835 39.0 35.0 41.0 30.0 41.0 54-55 36.18214182437629 39.0 35.0 41.0 29.5 41.0 56-57 35.983674576245505 39.0 35.0 41.0 29.0 41.0 58-59 35.77999997474457 39.0 35.0 40.5 28.5 41.0 60-61 35.549179893144284 38.0 35.0 40.0 28.0 41.0 62-63 35.59724917888293 38.0 35.0 40.0 29.0 41.0 64-65 35.39525690451807 38.0 35.0 40.0 29.0 41.0 66-67 35.120891099861225 37.0 34.5 40.0 28.0 41.0 68-69 34.810731094102984 37.0 34.0 39.5 28.0 41.0 70-71 34.48734482118526 36.0 34.0 39.0 28.0 41.0 72-73 34.138900744908824 36.0 34.0 39.0 28.0 41.0 74-75 33.793075340727285 35.0 34.0 38.5 27.5 40.0 76-77 33.47057679607127 35.0 34.0 37.0 27.0 39.0 78-79 33.141528572096036 35.0 33.0 37.0 26.5 39.0 80-81 32.836112482621104 35.0 33.0 36.0 26.0 39.0 82-83 32.564020613479585 35.0 33.0 36.0 26.0 37.0 84-85 32.33224335119313 35.0 33.0 35.5 26.0 37.0 86-87 32.133924478696414 35.0 33.0 35.0 26.0 36.5 88-89 31.953909476972733 35.0 33.0 35.0 26.0 36.0 90-91 31.820501156067174 35.0 33.0 35.0 25.0 36.0 92-93 31.687877016172312 35.0 33.0 35.0 25.0 36.0 94-95 31.58056639083531 35.0 33.0 35.0 25.0 35.5 96-97 31.523231520288316 35.0 33.0 35.0 25.0 35.0 98-99 31.446673481422742 35.0 32.5 35.0 25.0 35.0 100-101 31.434082388254204 35.0 32.5 35.0 25.0 35.0 102-103 30.3222463060781 33.5 30.0 34.5 22.0 35.0 104-105 31.271769862446316 35.0 32.0 35.0 24.5 35.0 106-107 31.4496498335036 35.0 33.0 35.0 25.0 35.0 108-109 31.450730450089594 35.0 33.0 35.0 25.0 35.0 110-111 31.3914666962997 35.0 33.0 35.0 25.0 35.0 112-113 31.327324225384483 35.0 33.0 35.0 24.0 35.0 114-115 31.209966675463974 35.0 33.0 35.0 24.0 35.0 116-117 31.11792137732997 35.0 33.0 35.0 23.5 35.0 118-119 31.00819443900751 35.0 33.0 35.0 23.0 35.0 120-121 30.874245020576858 35.0 33.0 35.0 20.0 35.0 122-123 30.679741927418426 35.0 33.0 35.0 18.5 35.0 124-125 29.651373453262938 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.278088248852459 1101 2 0.478292165377578 1101 3 0.33342980954225965 1101 4 0.19309186794897926 1101 5 0.20338072602615398 1101 6 0.2717308596786552 1101 7 0.2798364302344467 1101 8 0.25610518056669207 1101 9 0.40186111473136776 1101 10-11 0.3763900837909091 1101 12-13 0.34180187117318184 1101 14-15 0.3429455278148197 1101 16-17 0.4559694306985165 1101 18-19 0.3605938423633006 1101 20-21 0.3893416175281743 1101 22-23 0.3732929682285189 1101 24-25 0.43792522585111016 1101 26-27 0.44375621698841883 1101 28-29 0.4264918620698239 1101 30-31 0.39013227228134184 1101 32-33 0.29676153684786755 1101 34-35 0.37983238683870013 1101 36-37 0.37232139729644587 1101 38-39 0.3486795112890846 1101 40-41 0.38477737266887857 1101 42-43 0.37877983472496624 1101 44-45 0.4559831727767403 1101 46-47 0.520777740411603 1101 48-49 0.2880819411188327 1101 50-51 0.5027947096867678 1101 52-53 0.43212024019008766 1101 54-55 0.33682893466256303 1101 56-57 0.3934086351045991 1101 58-59 0.362991272985866 1101 60-61 0.4005240277873554 1101 62-63 0.5142649943024296 1101 64-65 0.5438890867234463 1101 66-67 0.5961493446269657 1101 68-69 0.659416733063594 1101 70-71 0.6460105688462647 1101 72-73 0.7459192471761327 1101 74-75 0.5543593307927992 1101 76-77 0.5831566771347241 1101 78-79 0.5923011080832268 1101 80-81 0.4864955939511759 1101 82-83 0.6394182351642357 1101 84-85 0.5011924478421008 1101 86-87 0.5695262264337941 1101 88-89 0.4977867993941878 1101 90-91 0.5395476768822185 1101 92-93 0.688203156541487 1101 94-95 0.6976958688689656 1101 96-97 0.4685014787759556 1101 98-99 0.5216409826186243 1101 100-101 0.5626078678990716 1101 102-103 0.611814859722724 1101 104-105 0.636742072058265 1101 106-107 0.6783985355159565 1101 108-109 0.6925341445644015 1101 110-111 0.7256277168893952 1101 112-113 0.7735361861400385 1101 114-115 0.7323674727844569 1101 116-117 0.738981904724799 1101 118-119 0.7467958519865974 1101 120-121 0.6619028019111717 1101 122-123 0.6871817808162284 1101 124-125 0.6268925703611004 1102 1 0.30968638486290345 1102 2 0.4674865017402219 1102 3 0.29309655019274317 1102 4 0.22485105716687315 1102 5 0.201065586169058 1102 6 0.2231109095145598 1102 7 0.24547673500751443 1102 8 0.2257662793220021 1102 9 0.3183072139975138 1102 10-11 0.3381054715692855 1102 12-13 0.378940237519906 1102 14-15 0.4348840095158977 1102 16-17 0.4103941410921337 1102 18-19 0.39229188169148443 1102 20-21 0.37417644488165536 1102 22-23 0.33413866868666275 1102 24-25 0.3606375531152537 1102 26-27 0.3574087980422149 1102 28-29 0.3514752912046575 1102 30-31 0.39918522405949375 1102 32-33 0.3474709007330006 1102 34-35 0.3304408911526622 1102 36-37 0.27806752409263424 1102 38-39 0.28606756043648573 1102 40-41 0.24960365413653562 1102 42-43 0.3278467578688691 1102 44-45 0.28756077450323403 1102 46-47 0.2634061589546661 1102 48-49 0.2530352533038709 1102 50-51 0.3453342469909728 1102 52-53 0.3333178721039829 1102 54-55 0.2329435763619614 1102 56-57 0.2577640875861036 1102 58-59 0.11460293746572603 1102 60-61 0.15588571554444997 1102 62-63 0.27091561101038764 1102 64-65 0.37505927416422224 1102 66-67 0.28775661034110556 1102 68-69 0.25722535253059675 1102 70-71 0.25237287514832474 1102 72-73 0.25577872321971995 1102 74-75 0.18738852520969118 1102 76-77 0.2398871158386271 1102 78-79 0.3187517319379296 1102 80-81 0.2823153572935553 1102 82-83 0.27010335047581435 1102 84-85 0.21022516731106577 1102 86-87 0.20570828903215244 1102 88-89 0.20442536227615093 1102 90-91 0.2757339671272767 1102 92-93 0.18254440111034498 1102 94-95 0.2015067903902512 1102 96-97 0.20684663693775462 1102 98-99 0.1951810982044968 1102 100-101 0.0613408321185851 1102 102-103 0.12543341828645183 1102 104-105 0.06454519055165164 1102 106-107 0.08686092531403844 1102 108-109 0.039166446502928665 1102 110-111 0.11162444247637282 1102 112-113 0.14681021162972385 1102 114-115 0.16571384136176803 1102 116-117 0.2026211233952928 1102 118-119 0.09656617501403275 1102 120-121 0.08521102604288089 1102 122-123 0.1202100693088255 1102 124-125 -0.11825431771346473 1103 1 0.35213409751873925 1103 2 0.4717475938720206 1103 3 0.32120310088367177 1103 4 0.23413662050260342 1103 5 0.16544975445771115 1103 6 0.09786121708717133 1103 7 0.220262847531977 1103 8 0.15497527928700805 1103 9 0.3050930330829047 1103 10-11 0.28866721222836134 1103 12-13 0.24966761982795305 1103 14-15 0.327804068230634 1103 16-17 0.2757476678798838 1103 18-19 0.2772207731917149 1103 20-21 0.2926703755385063 1103 22-23 0.1984376487839583 1103 24-25 0.2771526208081525 1103 26-27 0.3096816216799425 1103 28-29 0.28227927726494784 1103 30-31 0.31002517323261713 1103 32-33 0.29060353452371146 1103 34-35 0.32193797224145726 1103 36-37 0.2819880653665905 1103 38-39 0.2550390699247984 1103 40-41 0.25766532076281834 1103 42-43 0.2612307868412671 1103 44-45 0.3011155062756927 1103 46-47 0.3232944838482581 1103 48-49 0.3275879606158796 1103 50-51 0.38202776064127164 1103 52-53 0.34588233111270483 1103 54-55 0.22261120612798635 1103 56-57 0.10112154551527652 1103 58-59 0.09353404523862707 1103 60-61 0.16634511932469564 1103 62-63 0.3179015340109004 1103 64-65 0.45000645849511045 1103 66-67 0.381412050270896 1103 68-69 0.4527494979902258 1103 70-71 0.5357426528076488 1103 72-73 0.5247673780303757 1103 74-75 0.5451435109819371 1103 76-77 0.4105184069436518 1103 78-79 0.42085877259135174 1103 80-81 0.4600001229595705 1103 82-83 0.3889799129043041 1103 84-85 0.31003929451825485 1103 86-87 0.29998207105806785 1103 88-89 0.4041126746703192 1103 90-91 0.39777660934427317 1103 92-93 0.3315125185505856 1103 94-95 0.3340316351232886 1103 96-97 0.22392011381394994 1103 98-99 0.31811112442556677 1103 100-101 0.33902421808250693 1103 102-103 0.4082039509456834 1103 104-105 0.5246813065758467 1103 106-107 0.39920646598227094 1103 108-109 0.3750084820548274 1103 110-111 0.2526007196766962 1103 112-113 0.4076163076246857 1103 114-115 0.3876250417160456 1103 116-117 0.30655054860467246 1103 118-119 0.2542563652648582 1103 120-121 0.16224761776144092 1103 122-123 0.2990559720647319 1103 124-125 0.0919108225315739 1104 1 0.2987044343901992 1104 2 0.4227361692035565 1104 3 0.27330865356385203 1104 4 0.16722421528393738 1104 5 0.2176668104313748 1104 6 0.2162198065881853 1104 7 0.20227207041694584 1104 8 0.2880306321521502 1104 9 0.17962814141856853 1104 10-11 0.26072192025519314 1104 12-13 0.2742463360623191 1104 14-15 0.30036830105686363 1104 16-17 0.3670117348299442 1104 18-19 0.32355783343601274 1104 20-21 0.37053743137074946 1104 22-23 0.3285701057876622 1104 24-25 0.36905074342359256 1104 26-27 0.2635497854745381 1104 28-29 0.21037299871741766 1104 30-31 0.1693715815114345 1104 32-33 0.14410653740569046 1104 34-35 0.16064840518816936 1104 36-37 0.13348063275643796 1104 38-39 -0.018805806863007035 1104 40-41 0.0712121224495803 1104 42-43 -0.07241303402378207 1104 44-45 -0.07147943061217177 1104 46-47 0.07439272411841102 1104 48-49 -0.011040188273888418 1104 50-51 0.040489531344682916 1104 52-53 0.046704482299006145 1104 54-55 0.08631354324267448 1104 56-57 0.06656957734427493 1104 58-59 -0.19543077006326826 1104 60-61 -0.14655834653988364 1104 62-63 0.04007025539266351 1104 64-65 0.15437663458122586 1104 66-67 0.05907711686175787 1104 68-69 0.25992215769377225 1104 70-71 0.07621845440107933 1104 72-73 -0.05280262440191308 1104 74-75 -0.034766505082451715 1104 76-77 0.09036554773618377 1104 78-79 0.10329180697990381 1104 80-81 0.04802267533146676 1104 82-83 -0.00902612609144171 1104 84-85 0.030644972347978694 1104 86-87 0.08685337136888904 1104 88-89 0.2582922451740579 1104 90-91 0.02036279637001215 1104 92-93 0.010501112265675516 1104 94-95 0.15416690622406648 1104 96-97 0.20882562944420968 1104 98-99 0.1779606939677052 1104 100-101 0.14812821098928808 1104 102-103 0.08461292621839789 1104 104-105 0.20388122271858222 1104 106-107 0.23437408859347286 1104 108-109 0.11272791799098769 1104 110-111 0.2621074890649773 1104 112-113 0.17689216550005327 1104 114-115 0.24082047368701964 1104 116-117 0.19837662185038596 1104 118-119 0.1235730204771599 1104 120-121 0.25074734695762757 1104 122-123 0.15724651471880335 1104 124-125 0.01623476752799391 1105 1 0.27154044579873826 1105 2 0.43607972217774105 1105 3 0.2783233010371333 1105 4 0.17335905346956082 1105 5 0.1501113200209403 1105 6 0.1551554167434972 1105 7 0.2034721656916858 1105 8 0.07423910468228456 1105 9 0.21688555218501904 1105 10-11 0.28070416820973776 1105 12-13 0.2629698683122115 1105 14-15 0.2334184566926325 1105 16-17 0.348615363413181 1105 18-19 0.28182169560522397 1105 20-21 0.38506708359759045 1105 22-23 0.431696613540943 1105 24-25 0.42407750381779863 1105 26-27 0.4026615928241313 1105 28-29 0.3783069415647944 1105 30-31 0.34640701027829834 1105 32-33 0.3348224415690737 1105 34-35 0.44386427372531045 1105 36-37 0.32185462071680604 1105 38-39 0.3289078029706545 1105 40-41 0.3632261449368599 1105 42-43 0.3984793116280869 1105 44-45 0.4589173846057264 1105 46-47 0.34182718710886206 1105 48-49 0.31735738425603444 1105 50-51 0.41621096266442237 1105 52-53 0.3079817134685072 1105 54-55 0.2751450738883676 1105 56-57 0.1340662844619871 1105 58-59 0.22277393219299313 1105 60-61 0.25006245389007375 1105 62-63 0.42306045715931617 1105 64-65 0.4203789228036001 1105 66-67 0.4575385214312888 1105 68-69 0.5312309740680021 1105 70-71 0.42181688358935077 1105 72-73 0.39553525891096086 1105 74-75 0.5514986131661175 1105 76-77 0.5393894906039094 1105 78-79 0.38108205394097894 1105 80-81 0.453677780774953 1105 82-83 0.4540996996738613 1105 84-85 0.43157247600730386 1105 86-87 0.4102309524898402 1105 88-89 0.43387361448378314 1105 90-91 0.38618598640443835 1105 92-93 0.29399995492188324 1105 94-95 0.23739132505833638 1105 96-97 0.382788549655686 1105 98-99 0.4768981668663166 1105 100-101 0.44135632477338405 1105 102-103 0.46484463927042796 1105 104-105 0.48846259406409587 1105 106-107 0.40865688152766566 1105 108-109 0.4078981706929845 1105 110-111 0.577207059310755 1105 112-113 0.5339645335862748 1105 114-115 0.5286082064535869 1105 116-117 0.4133677356487304 1105 118-119 0.37975620855723236 1105 120-121 0.4182318902597899 1105 122-123 0.3335269217129486 1105 124-125 0.27989310376231913 1106 1 0.13029848990091963 1106 2 0.3895501989114898 1106 3 0.1466510785178059 1106 4 0.10536076479102263 1106 5 0.027870677324656867 1106 6 0.09567808283972568 1106 7 0.09663044510057262 1106 8 0.11737718099058725 1106 9 0.15816434903096166 1106 10-11 0.1905002951565109 1106 12-13 0.16802495147173602 1106 14-15 0.2480686756585868 1106 16-17 0.1144733118477319 1106 18-19 0.19489636983022507 1106 20-21 0.10062748195520044 1106 22-23 0.14471825462693033 1106 24-25 0.1265289123898583 1106 26-27 0.14951525701294344 1106 28-29 0.05807591316754923 1106 30-31 0.010662674574071218 1106 32-33 0.05123775789615337 1106 34-35 0.05818262705377464 1106 36-37 0.05178744540737057 1106 38-39 0.0886468858687266 1106 40-41 0.012387419158301327 1106 42-43 0.05486913639590796 1106 44-45 -0.015268697834812883 1106 46-47 0.0589317856910796 1106 48-49 0.2092292015249413 1106 50-51 0.2081249687610054 1106 52-53 0.053034924870182465 1106 54-55 0.060730013071093936 1106 56-57 0.025664022903136186 1106 58-59 -0.11779537929722039 1106 60-61 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76-77 -0.4112136087758387 2216 78-79 -0.36295537495758623 2216 80-81 -0.35446130557540556 2216 82-83 -0.46749401339778274 2216 84-85 -0.47512590695161805 2216 86-87 -0.4631847636064883 2216 88-89 -0.3606179758005048 2216 90-91 -0.31728275345461654 2216 92-93 -0.38953910571256145 2216 94-95 -0.3928444653692189 2216 96-97 -0.3907004874058444 2216 98-99 -0.3481890835515671 2216 100-101 -0.4887991034271799 2216 102-103 -0.4091189176076604 2216 104-105 -0.43539075175982234 2216 106-107 -0.42005661871418454 2216 108-109 -0.3845705875345793 2216 110-111 -0.5789116441174222 2216 112-113 -0.3898063070179276 2216 114-115 -0.41497291806653536 2216 116-117 -0.47573790164668495 2216 118-119 -0.4921678315350988 2216 120-121 -0.6515935476760788 2216 122-123 -0.6538681481792281 2216 124-125 -0.618286117780265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74.0 3 12.0 4 36.0 5 116.0 6 429.0 7 842.0 8 1364.0 9 2396.0 10 3827.0 11 5062.0 12 6553.0 13 9306.0 14 13918.0 15 10466.0 16 4909.0 17 4576.0 18 4500.0 19 4195.0 20 4143.0 21 4173.0 22 4629.0 23 5477.0 24 7102.0 25 9662.0 26 12756.0 27 16871.0 28 21572.0 29 27871.0 30 35011.0 31 43931.0 32 55059.0 33 70800.0 34 94600.0 35 136708.0 36 248551.0 37 483986.0 38 227503.0 39 832.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93888670619104 19.369639669571832 12.898523078555646 23.792950545681478 2 15.458700187404972 21.47517010410826 38.94872630286866 24.117403405618106 3 18.817961290713637 26.758390863527477 30.087018233293723 24.336629612465163 4 12.429738759307343 16.928606680497218 37.39708446262118 33.24457009757426 5 12.40996621226979 39.807957386164524 34.174858923124226 13.607217478441457 6 30.860742837876575 37.30807453886747 17.852935122596154 13.9782475006598 7 27.642688643504442 33.9458543386238 21.212165220290352 17.199291797581402 8 26.962315114489165 34.981039488123 20.026290899585685 18.030354497802143 9 27.699521030825515 14.863513358226765 19.89306852176197 37.54389708918575 10-11 24.694977579494616 27.042690511157215 27.815222456115542 20.44710945323263 12-13 25.47199236275885 24.749308316990966 28.386689632268354 21.392009687981826 14-15 22.62264351080743 25.319102321100022 26.732269743114422 25.32598442497812 16-17 21.724718370418824 29.10176548062972 27.11204822776354 22.061467921187912 18-19 21.665156599729134 27.105059618705468 29.91662536344207 21.31315841812333 20-21 22.656927753062536 26.231486193489406 29.883389379335252 21.22819667411281 22-23 22.390672413118175 26.55384015082541 29.496791929375725 21.558695506680692 24-25 21.929357391239837 26.56969631917819 29.969803970470082 21.531142319111893 26-27 21.856044510417608 26.969792443407 29.624121342914457 21.550041703260938 28-29 21.680020027673798 26.698777030177574 29.920410201107018 21.70079274104161 30-31 21.664093748914798 26.457865709478124 30.124068303863165 21.753972237743916 32-33 21.67553342618912 26.60018385950911 29.815799542624216 21.908483171677553 34-35 21.955331988902767 26.5727817211321 29.602864723093187 21.86902156687195 36-37 22.222060868104794 26.23988362299204 29.44325042397548 22.09480508492769 38-39 21.904859619242746 26.4249195377624 29.59517116081872 22.075049682176136 40-41 22.127376522484997 26.294694312825385 29.56818109351525 22.009748071174368 42-43 22.12438131666295 26.217935644936535 29.436983167772336 22.220699870628177 44-45 21.953785093994384 26.56940380776074 29.142016317531432 22.33479478071344 46-47 22.19948883015599 26.784264353606286 28.450396447066517 22.565850369171205 48-49 22.267268082570094 26.83641681051737 28.288004050970507 22.608311055942035 50-51 22.476856558013612 26.761218776399815 28.133503975835605 22.628420689750968 52-53 22.530871602671517 26.985297553127946 27.95958879113635 22.524242053064178 54-55 22.083818974149807 26.986149923791746 28.206744714354805 22.723286387703638 56-57 22.111234442558693 27.16670833838445 28.26463182405948 22.457425394997372 58-59 22.248410796230868 26.613038668730887 28.33653868009587 22.802011854942382 60-61 22.09683544843275 26.795566767615192 28.590636316655306 22.51696146729675 62-63 21.986528043005197 26.839640019610954 28.560176309193945 22.6136556281899 64-65 21.83549498742911 26.62897504637528 28.545893530696077 22.989636435499534 66-67 21.873051581417418 26.92666732541301 28.281085592933273 22.9191955002363 68-69 21.817121664231625 26.811950615537896 28.379554974119504 22.99137274611098 70-71 21.813396488737975 26.850907111801988 28.265432013021695 23.070264386438343 72-73 21.967359886047515 26.759072065098387 28.057990248879605 23.215577799974493 74-75 21.741702645127155 26.95960015607854 27.96429261442918 23.334404584365124 76-77 21.94106267260506 26.830450215870762 27.918359306435462 23.310127805088715 78-79 22.004895764538603 26.9129407545564 27.887326700416338 23.194836780488668 80-81 21.813333350170286 27.272293912558133 27.727838678434015 23.18653405883757 82-83 21.896493756382917 26.980949509649943 27.7267425066335 23.39581422733364 84-85 21.874577760828583 26.66247005653427 27.764711601964365 23.69824058067278 86-87 21.724307969728844 26.577738098695686 27.96265101166927 23.7353029199062 88-89 21.86406518930837 26.579095577900997 27.93679576820064 23.620043464589997 90-91 21.806720420857367 26.816271728791474 27.75246091003713 23.624546940314033 92-93 21.820340589343378 26.337475971760565 27.891320537424384 23.95086290147167 94-95 21.61625396472621 26.326981668577144 27.851564312776055 24.205200053920592 96-97 21.701469787834817 26.309663169937984 27.942543279752186 24.046323762475012 98-99 21.805905435863952 26.291070177526787 27.633358201627665 24.269666184981592 100-101 21.433738473891626 26.34727154521449 27.95694035678008 24.262049624113804 102-103 21.550498809411803 26.003128174980787 27.890294133061726 24.55607888254568 104-105 21.531442166656458 25.988745691394282 27.992598574647875 24.48721356730138 106-107 21.39578278351443 26.061084440627607 27.931379064836904 24.61175371102106 108-109 21.557436780201087 26.157329935173106 27.516257241402826 24.76897604322298 110-111 21.34852212666259 26.42630933727513 27.610868519929294 24.61430001613299 112-113 21.232967532549534 26.38138204503264 27.668304140831196 24.717346281586625 114-115 21.126005988258367 26.22369718758958 27.56739316287886 25.082903661273193 116-117 20.84497713752464 26.410673448590682 27.72414507222421 25.020204341660467 118-119 20.90767629451225 26.555168404426972 27.433693134481402 25.103462166579376 120-121 20.939386965414716 26.501987770625192 27.54655784044735 25.01206742351274 122-123 20.896774800496775 26.632662145498603 27.392539125614263 25.07802392839035 124-125 20.789348609757987 26.68560429617863 27.30884073600955 25.21620635805383 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 192.0 1 153.5 2 255.0 3 386.5 4 271.5 5 213.0 6 479.5 7 1056.0 8 1792.5 9 2096.0 10 1580.5 11 1070.5 12 976.5 13 1050.5 14 1239.5 15 1443.0 16 1638.5 17 1808.0 18 2024.0 19 2227.0 20 2461.0 21 2779.0 22 3263.0 23 4056.5 24 5120.0 25 6459.0 26 8349.5 27 10471.5 28 12404.0 29 15404.0 30 19618.5 31 22331.0 32 24892.0 33 28199.5 34 31881.5 35 34624.5 36 38122.0 37 42619.5 38 44892.0 39 46157.5 40 47915.0 41 51873.0 42 55259.5 43 58103.0 44 61334.0 45 62427.0 46 61258.0 47 60008.5 48 60181.5 49 59355.0 50 57623.0 51 55532.0 52 53692.0 53 52749.0 54 51268.0 55 47781.5 56 42231.0 57 37043.5 58 33814.5 59 31236.0 60 26983.5 61 21426.5 62 16406.0 63 12656.0 64 10198.0 65 8139.0 66 5861.5 67 4641.0 68 3267.5 69 1893.0 70 1386.0 71 1022.0 72 865.0 73 726.5 74 643.5 75 521.0 76 273.5 77 111.0 78 53.5 79 32.0 80 22.0 81 17.0 82 8.5 83 3.0 84 2.5 85 3.5 86 1.5 87 1.0 88 2.0 89 1.0 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04167145467471641 2 0.005177362550495069 3 7.576628122675712E-4 4 0.08403743359401143 5 0.0067558267427191765 6 0.0 7 0.005682471092006783 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.894157030668928E-4 20-21 0.0 22-23 0.0 24-25 3.1569283844482134E-5 26-27 5.682471092006784E-4 28-29 5.998163930451605E-4 30-31 8.208013799565353E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.1569283844482134E-5 40-41 0.0011049249345568746 42-43 6.945242445786069E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.313856768896427E-4 58-59 3.788314061337856E-4 60-61 4.7353925766723193E-4 62-63 5.998163930451605E-4 64-65 0.0 66-67 9.47078515334464E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.1569283844482134E-5 84-85 0.0 86-87 0.0 88-89 0.0 90-91 2.2098498691137494E-4 92-93 2.841235546003392E-4 94-95 4.7353925766723193E-4 96-97 0.0032832055198261413 98-99 0.008934107327988444 100-101 0.018531169616711012 102-103 0.009281369450277747 104-105 0.004356561170538534 106-107 0.012027897144747692 108-109 0.01363793062081628 110-111 0.006724257458874693 112-113 0.004924808279739213 114-115 0.0032832055198261413 116-117 0.0 118-119 0.0016731720437575528 120-121 5.366778253561962E-4 122-123 5.682471092006784E-4 124-125 0.034757781512774824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1583818.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.13110423053098 #Duplication Level Percentage of deduplicated Percentage of total 1 78.07430757610379 44.6047140385692 2 11.623242170625536 13.280973198934214 3 4.18832637212496 7.178511315520583 4 2.015929298603347 4.606890675195561 5 1.1134712212193356 3.1806920198589244 6 0.6913004534543099 2.369685495654691 7 0.44282359920854053 1.7709300841485407 8 0.31497908099673455 1.4396082165489041 9 0.23158176509447734 1.1907469763552634 >10 1.1943045552580573 12.173886574137555 >50 0.07394112209615887 2.914248906419738 >100 0.03414443326450178 3.422620147178037 >500 7.064365501019671E-4 0.25883892779626116 >1k 9.419154001359559E-4 1.6076534236825992 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4524 0.2856388802248743 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3923 0.2476926010438068 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2450 0.15468949083796243 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.313856768896427E-5 0.0 5 0.0 0.0 0.0 6.313856768896427E-5 0.0 6 0.0 0.0 0.0 6.313856768896427E-5 0.0 7 0.0 0.0 0.0 6.313856768896427E-5 0.0 8 0.0 0.0 0.0 6.313856768896427E-5 0.0 9 0.0 0.0 0.0 6.313856768896427E-5 0.0 10-11 0.0 0.0 0.0 6.313856768896427E-5 0.0 12-13 0.0 0.0 0.0 6.313856768896427E-5 0.0 14-15 0.0 0.0 0.0 6.313856768896427E-5 0.0 16-17 0.0 0.0 0.0 6.313856768896427E-5 0.0 18-19 0.0 0.0 0.0 6.313856768896427E-5 0.0 20-21 0.0 0.0 0.0 1.5784641922241067E-4 0.0 22-23 0.0 0.0 0.0 4.419699738227498E-4 0.0 24-25 0.0 0.0 0.0 0.0011364942184013567 0.0 26-27 0.0 0.0 0.0 0.002462404139869606 0.0 28-29 0.0 0.0 0.0 0.006503272471963318 0.0 30-31 0.0 0.0 0.0 0.020488465215068905 0.0 32-33 0.0 0.0 0.0 0.05088968555730519 0.0 34-35 0.0 0.0 0.0 0.09600219217107016 0.0 36-37 0.0 0.0 0.0 0.16709621938884392 0.0 38-39 0.0 0.0 0.0 0.27462120016315006 0.0 40-41 0.0 0.0 0.0 0.39022791760164366 0.0 42-43 0.0 0.0 0.0 0.5040351858610017 0.0 44-45 0.0 0.0 0.0 0.6328378639464888 0.0 46-47 0.0 0.0 0.0 0.7903054517627657 0.0 48-49 0.0 0.0 0.0 0.9615688166190812 0.0 50-51 0.0 0.0 0.0 1.1487115312491714 0.0 52-53 0.0 0.0 0.0 1.3306137447610773 0.0 54-55 0.0 0.0 0.0 1.51911393859648 0.0 56-57 0.0 0.0 0.0 1.7387098770186977 0.0 58-59 0.0 0.0 0.0 1.9882334965254844 0.0 60-61 0.0 0.0 0.0 2.2733672682088475 0.0 62-63 0.0 0.0 0.0 2.558248485621454 0.0 64-65 0.0 0.0 0.0 2.831228083024691 0.0 66-67 0.0 0.0 0.0 3.1255169470229536 0.0 68-69 0.0 0.0 0.0 3.4797243117580425 0.0 70-71 0.0 0.0 0.0 3.8755084233163153 0.0 72-73 0.0 0.0 0.0 4.271608227713033 0.0 74-75 0.0 0.0 0.0 4.654354225043534 0.0 76-77 0.0 0.0 0.0 5.061029739528153 0.0 78-79 0.0 0.0 0.0 5.510418495054356 0.0 80-81 0.0 0.0 0.0 6.035541962523472 0.0 82-83 0.0 0.0 0.0 6.5667583017745725 0.0 84-85 0.0 0.0 0.0 7.085788897461703 0.0 86-87 0.0 0.0 0.0 7.608702515061705 0.0 88-89 0.0 0.0 0.0 8.181022061878322 0.0 90-91 0.0 0.0 0.0 8.810923982427273 0.0 92-93 0.0 0.0 0.0 9.481013601310252 0.0 94-95 0.0 0.0 0.0 10.149524756001005 0.0 96-97 0.0 0.0 0.0 10.801872437363384 0.0 98-99 0.0 0.0 0.0 11.470573007757203 0.0 100-101 0.0 0.0 0.0 12.221353716146679 0.0 102-103 0.0 0.0 0.0 13.034010220871338 0.0 104-105 0.0 0.0 0.0 13.830155990145332 0.0 106-107 0.0 0.0 0.0 14.593911674194889 0.0 108-109 0.0 0.0 0.0 15.361771365144227 0.0 110-111 0.0 0.0 0.0 16.186739890568234 0.0 112-113 0.0 0.0 0.0 17.088453345018177 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3075 0.0 78.45957 1 GTATCAA 6255 0.0 60.475914 1 ATCAACG 8175 0.0 46.100426 3 TCAACGC 8275 0.0 45.54332 4 TATCAAC 8385 0.0 45.089287 2 CAACGCA 8430 0.0 44.68052 5 AACGCAG 8860 0.0 42.642334 6 GTATAGA 420 0.0 38.29574 1 GTACATG 11205 0.0 36.417885 1 ACGCAGA 10570 0.0 35.574844 7 CGCAGAG 10635 0.0 35.3563 8 TACATGG 11540 0.0 34.980545 2 ACATGGG 11850 0.0 33.5117 3 GCAGAGT 11865 0.0 31.440327 9 CATGGGG 3285 0.0 30.44829 4 TGGTATC 1195 0.0 30.392374 2 CATGGGA 9105 0.0 30.07924 4 TATCACG 80 0.0053455867 29.769579 2 GAGTACT 6235 0.0 28.435902 12-13 ATGGGAG 3310 0.0 26.78554 5 >>END_MODULE