FastQCFastQC Report
Thu 26 May 2016
SRR1364453_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364453_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581938
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26800.045.9755741
ATCAACG27350.043.706223
CAACGCA28250.042.7414675
GGTATCA16800.041.808471
TCAACGC30200.039.7785534
AACGCAG32100.037.4298556
TATCAAC35450.034.5585482
ACGCAGA36650.032.783047
CGCAGAG37550.031.8388928
GTACATG68000.031.3375991
TACATGG71850.028.9698222
ACATGGG71450.028.0499573
CATGGGG48400.026.5407354
TAAGGTG20700.022.700145
GGTAAGG17900.022.5922993
AGGTAAG17150.022.5399932
GTAAGGT18500.022.5025044
GTGTAAG16000.021.5774231
GCAGAGT54900.020.2600779
AGAGTAC44000.019.80417610-11
Produced by FastQC (version 0.11.5)