##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364453_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2581938 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.81607343011335 31.0 31.0 34.0 27.0 34.0 2 30.898280284034705 31.0 31.0 34.0 27.0 34.0 3 30.81356175090184 31.0 31.0 34.0 27.0 34.0 4 34.43896909995515 37.0 35.0 37.0 30.0 37.0 5 34.5875652320079 37.0 35.0 37.0 32.0 37.0 6 34.68300942935113 37.0 35.0 37.0 32.0 37.0 7 34.55686736087389 37.0 35.0 37.0 31.0 37.0 8 34.38500847038155 37.0 35.0 37.0 30.0 37.0 9 35.53575415056442 39.0 35.0 39.0 29.0 39.0 10-11 35.743630946986336 39.0 35.0 39.0 30.0 39.0 12-13 35.80445851139725 39.0 35.0 39.0 30.0 39.0 14-15 36.97657147460551 40.0 37.0 41.0 30.5 41.0 16-17 37.111083418734296 40.0 37.0 41.0 31.0 41.0 18-19 37.09501796712392 40.0 37.0 41.0 31.0 41.0 20-21 37.04266136522256 40.0 37.0 41.0 30.0 41.0 22-23 36.957090177998076 40.0 37.0 41.0 30.0 41.0 24-25 37.00199017172372 40.0 37.0 41.0 30.0 41.0 26-27 37.01095862875096 40.0 37.0 41.0 30.0 41.0 28-29 36.95564378385538 40.0 36.5 41.0 30.0 41.0 30-31 36.726437273087114 40.0 36.0 41.0 30.0 41.0 32-33 36.796240266032726 40.0 36.0 41.0 30.0 41.0 34-35 36.75086407961771 40.0 36.0 41.0 30.0 41.0 36-37 36.62368073904176 40.0 36.0 41.0 30.0 41.0 38-39 36.47834959631099 40.0 36.0 41.0 29.5 41.0 40-41 36.451595661863294 40.0 36.0 41.0 29.5 41.0 42-43 36.31854773429881 40.0 35.0 41.0 29.0 41.0 44-45 36.30592136604365 40.0 35.0 41.0 29.0 41.0 46-47 36.16515985279275 39.5 35.0 41.0 29.0 41.0 48-49 35.996623466558844 39.0 35.0 41.0 28.0 41.0 50-51 35.86618365739224 39.0 35.0 41.0 28.0 41.0 52-53 35.73947321740491 39.0 35.0 41.0 28.0 41.0 54-55 35.61951158393424 39.0 35.0 40.5 28.0 41.0 56-57 35.58262456340935 39.0 34.5 40.5 28.0 41.0 58-59 35.56182836303583 39.0 35.0 41.0 28.0 41.0 60-61 35.38534484561597 38.0 34.0 40.5 28.0 41.0 62-63 35.133974750749246 38.0 34.0 40.0 28.0 41.0 64-65 34.836499172327144 37.0 34.0 40.0 28.0 41.0 66-67 34.54061464682731 37.0 34.0 40.0 27.0 41.0 68-69 34.23770535930762 36.0 34.0 39.0 27.0 41.0 70-71 33.88025603248413 36.0 33.0 39.0 26.0 41.0 72-73 33.55397573450641 35.5 33.0 39.0 26.0 40.5 74-75 33.19370546465484 35.0 33.0 37.5 26.0 40.0 76-77 32.839251562198626 35.0 33.0 37.0 26.0 39.0 78-79 32.50509365445646 35.0 33.0 36.5 26.0 39.0 80-81 32.18577305109573 35.0 32.0 36.0 25.0 38.5 82-83 31.886408581460906 35.0 32.0 36.0 25.0 37.0 84-85 31.540626846965342 35.0 32.0 35.0 24.0 37.0 86-87 31.347148149955576 35.0 32.0 35.0 23.5 36.5 88-89 31.19922709220748 35.0 32.0 35.0 23.0 36.0 90-91 31.071348731069452 35.0 32.0 35.0 23.0 36.0 92-93 30.850197409852598 35.0 32.0 35.0 20.5 36.0 94-95 30.766346829397143 35.0 32.0 35.0 20.5 35.0 96-97 30.68794235183029 35.0 31.5 35.0 20.0 35.0 98-99 30.530350651332448 34.5 31.0 35.0 19.5 35.0 100-101 30.399568657341888 34.0 31.0 35.0 18.5 35.0 102-103 29.360284600172427 33.5 29.5 34.5 17.5 35.0 104-105 30.30603523399865 34.0 31.0 35.0 19.0 35.0 106-107 30.546221094387242 35.0 32.0 35.0 19.0 35.0 108-109 30.540403565073987 35.0 32.0 35.0 19.0 35.0 110-111 30.423663349003732 35.0 32.0 35.0 17.5 35.0 112-113 30.305885733894463 35.0 32.0 35.0 13.5 35.0 114-115 30.20673269458833 35.0 32.0 35.0 8.5 35.0 116-117 30.08037102362644 35.0 32.0 35.0 3.5 35.0 118-119 29.815381314346048 35.0 31.0 35.0 2.0 35.0 120-121 29.685153167891713 35.0 31.0 35.0 2.0 35.0 122-123 29.456403097208376 35.0 31.0 35.0 2.0 35.0 124-125 28.29202172941411 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7497288664152819 1101 2 0.7534518694729826 1101 3 0.9353124898239038 1101 4 0.8138530958804964 1101 5 0.683644547429104 1101 6 0.7312734446327482 1101 7 0.7329109279367501 1101 8 0.8640017148406614 1101 9 1.3170063675956882 1101 10-11 1.1177905305680724 1101 12-13 0.9572627903416375 1101 14-15 1.2163305948811924 1101 16-17 0.9366970452287475 1101 18-19 1.011933945919992 1101 20-21 1.0495821160016163 1101 22-23 1.0529913088920537 1101 24-25 1.1005959562039322 1101 26-27 1.0460683624158449 1101 28-29 1.0335376500246127 1101 30-31 1.1912501919813963 1101 32-33 1.009160832474322 1101 34-35 0.9781704674643805 1101 36-37 0.9618194980604642 1101 38-39 1.1227513804329732 1101 40-41 1.0669159546799705 1101 42-43 1.1100600840033152 1101 44-45 1.0194254542927936 1101 46-47 1.0535585954855122 1101 48-49 0.9430402595427836 1101 50-51 0.9939700719434157 1101 52-53 0.9172007536244706 1101 54-55 0.8589241172876925 1101 56-57 0.8651808876599958 1101 58-59 0.9123120486274061 1101 60-61 0.7723366497755677 1101 62-63 0.9037467108232065 1101 64-65 0.8312806906270112 1101 66-67 0.8714854055528676 1101 68-69 0.8222942716106374 1101 70-71 0.7359364736398533 1101 72-73 0.7513802216129122 1101 74-75 0.8317959675323436 1101 76-77 0.8156976047702926 1101 78-79 0.7538589477309756 1101 80-81 0.7840794014533756 1101 82-83 0.7810132078104637 1101 84-85 0.8824631257504798 1101 86-87 0.9039479052994359 1101 88-89 0.767637724610573 1101 90-91 0.7321682803099883 1101 92-93 0.8367686399034255 1101 94-95 0.8189799413786361 1101 96-97 0.7880086274868603 1101 98-99 0.7752804966838198 1101 100-101 0.7870143897352015 1101 102-103 0.8128948584742588 1101 104-105 0.7105848503374723 1101 106-107 0.6417180865006351 1101 108-109 0.6943181061370467 1101 110-111 0.7293073529225573 1101 112-113 0.6334340339742894 1101 114-115 0.629640355035999 1101 116-117 0.6657927214532116 1101 118-119 0.5553021508322118 1101 120-121 0.4298044035257327 1101 122-123 0.48197041060354096 1101 124-125 0.16421080973906754 1102 1 0.30790492877826736 1102 2 0.20661925470932374 1102 3 0.26465578016786395 1102 4 0.26275352515021666 1102 5 0.1580596604189637 1102 6 0.09447110152773774 1102 7 0.17873458101642825 1102 8 0.3721958428502745 1102 9 0.5633745173926599 1102 10-11 0.319967581393378 1102 12-13 0.3441891592589812 1102 14-15 0.4543660821762714 1102 16-17 0.3180565272595217 1102 18-19 0.26408637879160324 1102 20-21 0.36048159799494073 1102 22-23 0.3926586950644406 1102 24-25 0.3499793373067064 1102 26-27 0.30624386128042147 1102 28-29 0.27186377894031466 1102 30-31 0.3459543740381861 1102 32-33 0.19090802387469807 1102 34-35 0.1692579725892145 1102 36-37 0.13290117163201387 1102 38-39 0.22721317393046547 1102 40-41 0.11051968612524377 1102 42-43 0.1847205808928436 1102 44-45 0.08789987127787668 1102 46-47 0.14267465182037853 1102 48-49 0.10263269668275399 1102 50-51 0.028234425708092203 1102 52-53 0.01950051980071521 1102 54-55 -0.021060550313769966 1102 56-57 -0.0262937184059453 1102 58-59 0.08654210574480459 1102 60-61 0.03189084952357746 1102 62-63 0.037304959896992784 1102 64-65 0.04526312669989352 1102 66-67 0.038952311721949684 1102 68-69 0.12424494022676669 1102 70-71 0.20420575661152895 1102 72-73 0.10899507887558002 1102 74-75 0.09717797639481063 1102 76-77 0.12972277124569587 1102 78-79 0.047772041674207344 1102 80-81 0.18497950123565232 1102 82-83 0.11315854546956317 1102 84-85 0.23287910364486564 1102 86-87 0.1921742987411399 1102 88-89 0.12052509232417208 1102 90-91 0.0072289827547464824 1102 92-93 0.03904537940054453 1102 94-95 0.1406532882783651 1102 96-97 0.034388243821201314 1102 98-99 0.021457459521009525 1102 100-101 -0.016908523308192258 1102 102-103 -0.12812735759087346 1102 104-105 -0.054786394020524654 1102 106-107 -0.1094280992142096 1102 108-109 -0.12232681311044757 1102 110-111 -0.14659592940886412 1102 112-113 -0.2034689806485872 1102 114-115 -0.2984733480845847 1102 116-117 -0.4372495615203391 1102 118-119 -0.21416685956348758 1102 120-121 -0.252717598185356 1102 122-123 -0.22139120373515553 1102 124-125 -0.34432591032578586 1103 1 0.5090321591932039 1103 2 0.5186312229013161 1103 3 0.6304319621387577 1103 4 0.5843899188975783 1103 5 0.5206536389931671 1103 6 0.4139587392690274 1103 7 0.46003105782799025 1103 8 0.5651418058107041 1103 9 0.9619543937188269 1103 10-11 0.753485882434461 1103 12-13 0.5896470076061391 1103 14-15 0.8192442181894393 1103 16-17 0.6407327743417781 1103 18-19 0.6401866502957603 1103 20-21 0.6555020349801381 1103 22-23 0.6176034960540022 1103 24-25 0.5813251601185527 1103 26-27 0.6606923782571243 1103 28-29 0.5496723202353309 1103 30-31 0.7628068355661028 1103 32-33 0.6326304514767358 1103 34-35 0.5539917712143776 1103 36-37 0.547769813361441 1103 38-39 0.6266420738828273 1103 40-41 0.6078393041745187 1103 42-43 0.649215533548194 1103 44-45 0.5721543299499601 1103 46-47 0.5253066938412161 1103 48-49 0.45559368596553895 1103 50-51 0.45802568093331075 1103 52-53 0.38977165172971695 1103 54-55 0.2668228555663177 1103 56-57 0.2834714491169237 1103 58-59 0.3482715920231314 1103 60-61 0.35190269580034084 1103 62-63 0.3605511337276184 1103 64-65 0.42839968740640444 1103 66-67 0.2767083944020641 1103 68-69 0.2996624189873529 1103 70-71 0.3423352451555459 1103 72-73 0.3127198185514928 1103 74-75 0.31732991730152094 1103 76-77 0.2712885431660226 1103 78-79 0.28441787306327626 1103 80-81 0.3643688677457604 1103 82-83 0.33199126643958365 1103 84-85 0.4279285089844258 1103 86-87 0.4540612363868952 1103 88-89 0.45829172121150563 1103 90-91 0.5176172665928327 1103 92-93 0.46080196214889213 1103 94-95 0.4295264462659425 1103 96-97 0.4119860902371357 1103 98-99 0.4741039776273084 1103 100-101 0.4670783017547713 1103 102-103 0.4301087447467147 1103 104-105 0.42801020659433675 1103 106-107 0.5130584105414364 1103 108-109 0.48183505471725496 1103 110-111 0.5091879369979324 1103 112-113 0.5143191668170601 1103 114-115 0.47124253008001915 1103 116-117 0.371234889162519 1103 118-119 0.3209450650950494 1103 120-121 0.25426676915279955 1103 122-123 0.2442872302690553 1103 124-125 0.029203352297436425 1104 1 0.39863239225536873 1104 2 0.4368799535448815 1104 3 0.642985262465487 1104 4 0.5190931334064359 1104 5 0.4813329593619784 1104 6 0.31922336808170826 1104 7 0.547618495650859 1104 8 0.5234812852981747 1104 9 1.0278492439089533 1104 10-11 0.7490096822749379 1104 12-13 0.6470106446402681 1104 14-15 0.7037206000995226 1104 16-17 0.604333734932986 1104 18-19 0.5826313191040953 1104 20-21 0.6912564525723326 1104 22-23 0.7599326874062555 1104 24-25 0.7646707111283035 1104 26-27 0.6741699723644032 1104 28-29 0.7303729396623027 1104 30-31 0.7689325537442215 1104 32-33 0.717276822840013 1104 34-35 0.6358486007681776 1104 36-37 0.5285491650229588 1104 38-39 0.69682175933319 1104 40-41 0.6523277580093492 1104 42-43 0.6223890194606838 1104 44-45 0.5555827618482923 1104 46-47 0.6273021437555002 1104 48-49 0.5749879324277245 1104 50-51 0.5843419895952806 1104 52-53 0.3986678147383742 1104 54-55 0.39572548784121153 1104 56-57 0.49432573732378415 1104 58-59 0.48819561765576225 1104 60-61 0.5865264010251678 1104 62-63 0.4869282893155784 1104 64-65 0.38940727825797694 1104 66-67 0.3973550734242579 1104 68-69 0.4315861274784254 1104 70-71 0.4309945503099897 1104 72-73 0.43306318486684603 1104 74-75 0.4540252364607724 1104 76-77 0.47567475045859453 1104 78-79 0.29776606867534383 1104 80-81 0.3262199524014804 1104 82-83 0.3252229678460701 1104 84-85 0.3595560562580111 1104 86-87 0.4433780571113033 1104 88-89 0.5092057816454094 1104 90-91 0.45855129851560505 1104 92-93 0.5030814223339313 1104 94-95 0.5068709889551961 1104 96-97 0.39090539615019537 1104 98-99 0.5115685626091278 1104 100-101 0.4652669862284249 1104 102-103 0.44988478591400494 1104 104-105 0.5176273240199478 1104 106-107 0.4184997851867571 1104 108-109 0.2545100831388254 1104 110-111 0.4500686949346395 1104 112-113 0.47058181691931367 1104 114-115 0.4567064365264244 1104 116-117 0.4082212594510999 1104 118-119 0.25566790339605916 1104 120-121 0.10716142336841017 1104 122-123 0.2037511925576574 1104 124-125 -0.016984600959482066 1105 1 0.13885853510361557 1105 2 0.2166843760357402 1105 3 0.2675028617906179 1105 4 0.18847167935378195 1105 5 0.132378478221284 1105 6 0.23466859212201285 1105 7 0.1871294399312049 1105 8 0.3325060124181789 1105 9 0.7818488331799713 1105 10-11 0.33584907386076424 1105 12-13 0.3905857985220962 1105 14-15 0.06430529313622202 1105 16-17 0.18447410722432522 1105 18-19 0.1907053797674294 1105 20-21 0.4731336924735672 1105 22-23 0.5386449529677648 1105 24-25 0.5648292831399573 1105 26-27 0.4832344599027891 1105 28-29 0.4283665697602004 1105 30-31 0.5240206327215304 1105 32-33 0.44875123437294917 1105 34-35 0.4089815403060868 1105 36-37 0.4084276010822876 1105 38-39 0.4577013164047088 1105 40-41 0.3560226332131009 1105 42-43 0.46382710875769817 1105 44-45 0.32143500904536637 1105 46-47 0.4051707550025796 1105 48-49 0.2651481488993994 1105 50-51 0.3075818447695653 1105 52-53 0.23509441855011914 1105 54-55 0.17118380065096517 1105 56-57 0.2887497375358379 1105 58-59 0.4440637085431902 1105 60-61 0.3860022261760889 1105 62-63 0.42269401449671307 1105 64-65 0.37705841931764894 1105 66-67 0.1978013294339931 1105 68-69 0.18724166097059225 1105 70-71 0.2844268073069287 1105 72-73 0.26030146480798066 1105 74-75 0.2628632247225724 1105 76-77 0.23793173581080396 1105 78-79 0.11775440947951665 1105 80-81 0.1495219509222352 1105 82-83 0.11417264042094999 1105 84-85 0.32523342195143456 1105 86-87 0.25061539901538765 1105 88-89 0.2448322295551435 1105 90-91 0.14800477033357495 1105 92-93 0.09948110358147844 1105 94-95 0.08940502850562027 1105 96-97 0.024515328174032902 1105 98-99 0.09225776378243467 1105 100-101 -0.053558671156689996 1105 102-103 -0.03264649356853866 1105 104-105 -0.07533234115872744 1105 106-107 -0.20718334769744828 1105 108-109 -0.22484586370728366 1105 110-111 -0.22335852960286928 1105 112-113 -0.14009819174820137 1105 114-115 -0.173510735136162 1105 116-117 -0.1982409923422388 1105 118-119 -0.31086581678427194 1105 120-121 -0.3999108484621168 1105 122-123 -0.2728160885039763 1105 124-125 -0.48576926265108256 1106 1 0.2627565120534108 1106 2 0.3223972185720392 1106 3 0.3803862437887737 1106 4 0.19639336864453583 1106 5 0.17925444055975248 1106 6 0.18119521455843568 1106 7 0.10395038581097538 1106 8 0.39350867798535205 1106 9 0.8646457616605048 1106 10-11 0.2902826464561272 1106 12-13 0.4069088892827111 1106 14-15 0.6718609445978174 1106 16-17 0.5125518322216678 1106 18-19 0.5080665678120937 1106 20-21 0.5634706020533287 1106 22-23 0.5012993004175925 1106 24-25 0.38322298108536756 1106 26-27 0.4858711268167184 1106 28-29 0.38222611899965386 1106 30-31 0.45784010051363566 1106 32-33 0.4257254030008255 1106 34-35 0.39548589027666026 1106 36-37 0.3635774505279983 1106 38-39 0.4766500277582395 1106 40-41 0.4176490948035365 1106 42-43 0.4477405826683025 1106 44-45 0.21894727479673293 1106 46-47 0.34428661260650983 1106 48-49 0.23061381715847062 1106 50-51 0.26546209430082257 1106 52-53 0.19802530936205187 1106 54-55 0.07063167726870034 1106 56-57 0.09160261049448337 1106 58-59 0.15045378046951186 1106 60-61 0.10202989207240876 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2216 72-73 -1.5905286014619264 2216 74-75 -1.4034962298772093 2216 76-77 -1.380954903556784 2216 78-79 -1.3973754525258144 2216 80-81 -1.2967056585726837 2216 82-83 -1.532647701395227 2216 84-85 -1.6589397956780552 2216 86-87 -1.7029007658154534 2216 88-89 -1.9837747520185118 2216 90-91 -1.699953434647675 2216 92-93 -1.5189580535427432 2216 94-95 -1.5348620310238736 2216 96-97 -1.5189495525334067 2216 98-99 -1.5403650740808388 2216 100-101 -1.5580326074869681 2216 102-103 -1.807282562019676 2216 104-105 -1.7164249102597324 2216 106-107 -1.3859395668145318 2216 108-109 -1.4588712792809417 2216 110-111 -1.5179757878499238 2216 112-113 -1.629374479159047 2216 114-115 -1.5033358962917944 2216 116-117 -1.4779960804368706 2216 118-119 -1.2901348062470852 2216 120-121 -1.2043017391768522 2216 122-123 -1.0585560079252758 2216 124-125 -1.0185791037869087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34896.0 3 7233.0 4 4641.0 5 4017.0 6 4368.0 7 5282.0 8 6083.0 9 6818.0 10 7693.0 11 8279.0 12 9297.0 13 9844.0 14 9917.0 15 9554.0 16 9019.0 17 8476.0 18 8016.0 19 8053.0 20 8234.0 21 8853.0 22 10141.0 23 13387.0 24 18130.0 25 25017.0 26 30224.0 27 34816.0 28 41761.0 29 50067.0 30 60699.0 31 73829.0 32 92827.0 33 120888.0 34 164760.0 35 239159.0 36 423895.0 37 715143.0 38 288030.0 39 592.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.38617836856572 18.687039211437384 12.918922921089857 25.007859498907038 2 15.34299769125928 19.98278804939102 39.93830964237176 24.735904616977937 3 18.215679325776986 24.391125260705635 30.1455303870934 27.247665026423977 4 12.214768167982303 16.386013197303836 38.25479077157014 33.14442786314372 5 13.084455660942613 38.3071298739577 35.40981854138272 13.198595923716969 6 32.42396297657608 37.1853181318028 17.418383823282788 12.972335068338333 7 28.177709921771942 32.67828274730067 21.765627214906015 17.37838011602138 8 25.939510508334358 35.416512549077176 20.520600502807426 18.123376439781044 9 26.002637577147198 16.0641616784441 20.340212594140397 37.59298815026831 10-11 24.317255849886248 26.427593804149254 28.809729884876045 20.44542046108846 12-13 25.115163064159578 23.81680808479859 28.192394502848213 22.875634348193614 14-15 22.094192655545466 25.61974076094608 25.342320772647103 26.943745810861348 16-17 22.63558238201732 28.635593226903683 26.439813591896083 22.289010799182915 18-19 22.610252497931988 27.726275190188353 27.463936406522272 22.199535905357386 20-21 22.886686754583746 27.213943289629167 27.57917873255534 22.32019122323174 22-23 22.83327303672567 27.08323488330476 27.29253641618139 22.790955663788182 24-25 22.64066129249984 27.322841035352486 27.29202766502245 22.74447000712523 26-27 22.356846068004582 27.608893282239972 27.062465937864467 22.971794711890983 28-29 22.594656086725973 27.753384247296687 26.99759580840991 22.654363857567432 30-31 22.701387808797673 27.26848369913965 26.956836359192053 23.07329213287062 32-33 22.279443661208113 27.470079941747873 26.980649759090277 23.269826637953738 34-35 23.187699937120083 27.49196281583505 26.632345761686476 22.687991485358392 36-37 23.344143765907496 26.882615821291093 26.76855339602958 23.004687016771825 38-39 22.580154779773938 27.284026094468604 26.873867246027338 23.261951879730116 40-41 23.047310045902357 26.95268027128152 27.124104847856817 22.875904834959304 42-43 22.732116834262087 26.91742879413602 26.89231126754426 23.458143104057633 44-45 22.634436994523234 27.280848473792524 27.163047926019008 22.921666605665234 46-47 23.23109752330705 27.50219267625796 26.34193820380841 22.92477159662658 48-49 22.64143087027514 27.443618705987593 26.62976504703533 23.285185376701936 50-51 22.928923092296913 27.371815653288063 26.853225780838162 22.84603547357686 52-53 23.193328055231056 27.033844668858475 26.8566576907941 22.91616958511637 54-55 22.588141421360007 27.35827990475333 26.91806907610922 23.13550959777745 56-57 22.638985482911043 27.592152633855104 26.79309976850359 22.975762114730262 58-59 22.603426324418983 27.01446363021199 27.013805153701973 23.36830489166706 60-61 22.39750548338522 27.246164051265414 27.285518536989244 23.070811928360115 62-63 22.484897444394452 27.188647968052365 27.503975732874135 22.822478854679044 64-65 22.3014867012237 27.3361292702837 27.028543927552246 23.33384010094036 66-67 22.30506944841217 27.453422645130864 26.91487909110365 23.326628815353313 68-69 22.809975395027564 26.943645626419666 27.414789013910795 22.831589964641974 70-71 22.646904179307096 26.844551001180367 27.02800632497717 23.480538494535363 72-73 22.50622192869137 27.02371983211929 26.95455716330408 23.515501075885258 74-75 22.475539939955993 27.472447873058886 26.89166610015895 23.160346086826166 76-77 22.79216249495021 27.06212470335515 27.165560651501224 22.980152150193412 78-79 22.773680077234957 27.010796044903955 27.133484975689775 23.082038902171316 80-81 22.882172831051246 27.18643797302477 26.93093656798009 23.000452627943897 82-83 22.90257558270048 27.227998056163997 26.952421146905852 22.91700521422967 84-85 22.690971237017983 27.256784020914164 27.070794675936373 22.98145006613148 86-87 22.950345307652782 27.173137259041457 26.967232911692 22.90928452161376 88-89 22.753907649661695 27.434642668578707 26.72973369761216 23.08171598414744 90-91 22.624769597949513 27.366408451099762 27.00080588667192 23.00801606427881 92-93 22.848798418872818 26.796271065314457 27.25217177424587 23.102758741566852 94-95 22.494030674267997 26.75969951278992 27.51775401129587 23.228515801646214 96-97 22.818152573947724 26.48255194095531 27.492349534058008 23.206945951038954 98-99 22.474497176940034 26.858751718295913 27.191817406100384 23.47493369866367 100-101 22.48794672271323 26.60062525589126 27.5945776036609 23.316850417734617 102-103 22.397179474416813 26.332652437117165 27.660177332566636 23.609990755899386 104-105 22.192609584191306 26.785880291346526 27.362716662250918 23.658793462211253 106-107 22.533340640906438 26.640655063247625 26.97939206285236 23.84661223299358 108-109 22.53110812573119 26.57250648422841 26.99716462067869 23.899220769361712 110-111 22.477047696403275 26.670900201296245 27.324812812310327 23.527239289990153 112-113 22.512867450540494 26.78889324431733 27.487713560170246 23.21052574497193 114-115 22.291374131108867 27.14432356740697 27.185247879702278 23.379054421781884 116-117 22.36428636862699 26.969833539176463 27.349260175193578 23.316619917002964 118-119 22.174124171896878 27.234602337767093 27.00804939331633 23.583224097019702 120-121 22.17707177964759 27.353697650786618 27.20121321206378 23.268017357502014 122-123 22.236922391500162 27.223469476701958 27.29773653982121 23.24187159197668 124-125 22.139090146031027 27.319049101738752 27.240431345539985 23.301429406690236 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 363.0 1 327.0 2 265.0 3 225.5 4 181.5 5 147.0 6 136.0 7 139.0 8 144.5 9 133.0 10 123.5 11 121.0 12 139.0 13 161.0 14 169.5 15 183.0 16 212.0 17 278.5 18 355.5 19 451.0 20 624.0 21 870.0 22 1270.0 23 1884.5 24 2814.5 25 4293.0 26 6883.5 27 10626.5 28 14817.5 29 19439.0 30 24776.0 31 31904.0 32 42798.5 33 49585.5 34 51497.0 35 57218.0 36 66562.5 37 74447.5 38 79906.0 39 84550.5 40 87195.0 41 89483.0 42 91017.5 43 93444.0 44 96325.0 45 100911.5 46 102254.5 47 100121.5 48 98330.5 49 94380.5 50 92008.5 51 90186.5 52 89664.0 53 91372.0 54 93105.5 55 87567.5 56 77445.0 57 69997.5 58 64333.5 59 54828.5 60 41937.5 61 34512.0 62 29980.0 63 21968.0 64 14450.0 65 10920.5 66 8261.5 67 6693.5 68 5354.5 69 3855.5 70 2791.0 71 1838.0 72 1267.0 73 900.5 74 727.0 75 606.5 76 427.0 77 235.5 78 118.0 79 70.5 80 47.0 81 29.5 82 22.5 83 22.0 84 18.5 85 12.0 86 8.0 87 7.0 88 5.5 89 7.0 90 6.0 91 5.0 92 4.0 93 4.0 94 4.5 95 2.0 96 1.0 97 2.0 98 1.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08741495729177076 2 4.260365663311822E-4 3 1.1619179081759516E-4 4 0.001820338056142324 5 0.0010457261173583564 6 1.9365298469599192E-4 7 0.0 8 0.0013555708928719434 9 8.908037296015629E-4 10-11 0.009392169757755609 12-13 0.0021495481301255105 14-15 0.0043571921556598186 16-17 0.0029435253673790775 18-19 0.01365253542106743 20-21 0.01752559511498727 22-23 0.009915032816434787 24-25 0.01032170408429637 26-27 0.010031224607252382 28-29 0.007629927597022082 30-31 0.019152280186433602 32-33 0.002246374622473506 34-35 0.0012587444005239476 36-37 7.165160433751702E-4 38-39 4.4540186480078144E-4 40-41 0.0017235115637943283 42-43 0.0029241600689094783 44-45 0.0012200138035847491 46-47 0.0018397033546119232 48-49 0.008075329461822864 50-51 0.004996247005156592 52-53 0.007823580581718073 54-55 0.008540096625093245 56-57 0.009934398114904387 58-59 0.008559461923562843 60-61 0.010670279456749155 62-63 0.013110307063918653 64-65 0.008346443640397251 66-67 0.013381421242493044 68-69 0.01340078654096264 70-71 0.004279730961781422 72-73 0.013284594750145045 74-75 0.012839192885344264 76-77 0.005693397750062162 78-79 0.007959137671005269 80-81 0.013458882436371437 82-83 0.017215750339473684 84-85 0.013110307063918653 86-87 0.015472873477209754 88-89 0.015453508178740156 90-91 0.0119871197526819 92-93 0.00912105557918122 94-95 0.007649292895491681 96-97 0.009004863788363624 98-99 0.006390548494967733 100-101 0.010650914158279555 102-103 0.011289969007776329 104-105 0.010457261173583563 106-107 0.005480379466896571 108-109 0.003737502604632644 110-111 0.0066229320766029234 112-113 0.008908037296015628 114-115 0.013284594750145045 116-117 0.010825201844505949 118-119 0.22150028389527557 120-121 0.026762842484986088 122-123 0.02761491561764845 124-125 0.2395100114720028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2581938.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.20881613387787 #Duplication Level Percentage of deduplicated Percentage of total 1 83.8724364017487 53.01477411218498 2 8.931286669583036 11.290721138732572 3 2.9499411463965854 5.59386862585028 4 1.3267282108625262 3.354436781601526 5 0.7560756260370812 2.389532261474223 6 0.4764678033739709 1.8070179466306797 7 0.3182137295301461 1.4079739184802582 8 0.22761073988377156 1.1509604325927376 9 0.16193274445292255 0.921201936316111 >10 0.851646794002804 10.064507635784219 >50 0.08231669786296131 3.671296461840381 >100 0.043891155596366214 4.74855424288654 >500 0.0014522806691706848 0.5851545056255218 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.130182831655911E-4 14-15 0.0 0.0 0.0 0.0 2.517488801047895E-4 16-17 0.0 0.0 0.0 0.0 2.711141785743887E-4 18-19 0.0 0.0 0.0 0.0 3.098447755135871E-4 20-21 0.0 0.0 0.0 3.8730596939198386E-5 3.8730596939198385E-4 22-23 0.0 0.0 0.0 1.1619179081759516E-4 3.8730596939198385E-4 24-25 1.9365298469599193E-5 0.0 0.0 1.9365298469599192E-4 3.8730596939198385E-4 26-27 3.8730596939198386E-5 0.0 0.0 5.228630586791782E-4 4.06671267861583E-4 28-29 3.8730596939198386E-5 0.0 0.0 0.002285105219412705 4.260365663311822E-4 30-31 3.8730596939198386E-5 0.0 0.0 0.01006995520419158 4.4540186480078144E-4 32-33 3.8730596939198386E-5 0.0 0.0 0.028195874571736425 4.647671632703806E-4 34-35 3.8730596939198386E-5 0.0 0.0 0.054184105117938544 5.03497760209579E-4 36-37 3.8730596939198386E-5 0.0 0.0 0.09953763413373984 5.03497760209579E-4 38-39 3.8730596939198386E-5 0.0 0.0 0.167993964223773 5.03497760209579E-4 40-41 3.8730596939198386E-5 0.0 0.0 0.23842555475770527 5.03497760209579E-4 42-43 3.8730596939198386E-5 0.0 0.0 0.31548007736824046 5.422283571487774E-4 44-45 3.8730596939198386E-5 0.0 0.0 0.4088401812901782 6.196895510271741E-4 46-47 3.8730596939198386E-5 0.0 0.0 0.517750619883204 6.584201479663725E-4 48-49 3.8730596939198386E-5 0.0 0.0 0.6360532282339855 6.584201479663725E-4 50-51 3.8730596939198386E-5 0.0 0.0 0.7709325320747439 6.584201479663725E-4 52-53 3.8730596939198386E-5 0.0 0.0 0.8980850818261321 7.358813418447694E-4 54-55 3.8730596939198386E-5 0.0 0.0 1.036275851705192 7.746119387839677E-4 56-57 3.8730596939198386E-5 0.0 0.0 1.197453230867666 7.746119387839677E-4 58-59 3.8730596939198386E-5 0.0 0.0 1.3772212965609554 7.746119387839677E-4 60-61 3.8730596939198386E-5 0.0 0.0 1.572539696925333 7.746119387839677E-4 62-63 3.8730596939198386E-5 0.0 0.0 1.761777393570256 8.133425357231661E-4 64-65 3.8730596939198386E-5 0.0 0.0 1.9609688536285534 8.908037296015629E-4 66-67 3.8730596939198386E-5 0.0 0.0 2.2000721938326944 8.908037296015629E-4 68-69 3.8730596939198386E-5 0.0 0.0 2.46506693809069 8.908037296015629E-4 70-71 3.8730596939198386E-5 0.0 0.0 2.74288150993556 9.101690280711621E-4 72-73 3.8730596939198386E-5 0.0 0.0 3.0278031463187727 9.488996250103604E-4 74-75 3.8730596939198386E-5 0.0 0.0 3.318747390526031 9.682649234799596E-4 76-77 3.8730596939198386E-5 0.0 0.0 3.6384878335575834 9.682649234799596E-4 78-79 3.8730596939198386E-5 0.0 0.0 4.002845924263092 9.682649234799596E-4 80-81 3.8730596939198386E-5 0.0 0.0 4.385523587320842 9.682649234799596E-4 82-83 3.8730596939198386E-5 0.0 0.0 4.787992585414521 9.682649234799596E-4 84-85 3.8730596939198386E-5 0.0 0.0 5.1959225976766295 9.682649234799596E-4 86-87 3.8730596939198386E-5 0.0 0.0 5.628194790115022 0.0010263608188887572 88-89 3.8730596939198386E-5 0.0 0.0 6.084073281387857 0.0010844567142975548 90-91 3.8730596939198386E-5 0.0 0.0 6.572950241252888 0.0010844567142975548 92-93 3.8730596939198386E-5 0.0 0.0 7.076932133924207 0.0011619179081759515 94-95 3.8730596939198386E-5 0.0 0.0 7.592397648587999 0.0011619179081759515 96-97 3.8730596939198386E-5 0.0 0.0 8.125563046053003 0.0012974749974631458 98-99 5.809589540879758E-5 3.8730596939198386E-5 0.0 8.687892583013225 0.0013943014898111419 100-101 7.746119387839677E-5 3.8730596939198386E-5 0.0 9.307582134040398 0.0013943014898111419 102-103 7.746119387839677E-5 3.8730596939198386E-5 0.0 9.941737563024365 0.001413666788280741 104-105 7.746119387839677E-5 3.8730596939198386E-5 0.0 10.58166385095227 0.0014717626836895387 106-107 7.746119387839677E-5 3.8730596939198386E-5 0.0 11.236578879895646 0.0014717626836895387 108-109 7.746119387839677E-5 3.8730596939198386E-5 0.0 11.923039980045996 0.001510493280628737 110-111 7.746119387839677E-5 3.8730596939198386E-5 0.0 12.64999391929628 0.0015492238775679354 112-113 7.746119387839677E-5 3.8730596939198386E-5 0.0 13.398791915220272 0.0015492238775679354 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2680 0.0 45.975574 1 ATCAACG 2735 0.0 43.70622 3 CAACGCA 2825 0.0 42.741467 5 GGTATCA 1680 0.0 41.80847 1 TCAACGC 3020 0.0 39.778553 4 AACGCAG 3210 0.0 37.429855 6 TATCAAC 3545 0.0 34.558548 2 ACGCAGA 3665 0.0 32.78304 7 CGCAGAG 3755 0.0 31.838892 8 GTACATG 6800 0.0 31.337599 1 TACATGG 7185 0.0 28.969822 2 ACATGGG 7145 0.0 28.049957 3 CATGGGG 4840 0.0 26.540735 4 TAAGGTG 2070 0.0 22.70014 5 GGTAAGG 1790 0.0 22.592299 3 AGGTAAG 1715 0.0 22.539993 2 GTAAGGT 1850 0.0 22.502504 4 GTGTAAG 1600 0.0 21.577423 1 GCAGAGT 5490 0.0 20.260077 9 AGAGTAC 4400 0.0 19.804176 10-11 >>END_MODULE