FastQCFastQC Report
Thu 26 May 2016
SRR1364453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364453_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581938
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33010.12784970049629388No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27750.068.7089841
GTATCAA50800.051.725111
ATCAACG60100.043.105313
CAACGCA61700.041.851235
TCAACGC62900.041.1864784
TATCAAC66050.039.6738432
AACGCAG66250.038.9746556
ACGCAGA76650.033.608897
CGCAGAG77200.033.369458
GTACATG70750.029.3075371
TACATGG71400.027.8594592
ACATGGG73450.026.51383
GCAGAGT95550.026.1515279
TAAGGTA12800.024.6593694
CAGAGTA87550.024.49782610-11
AGAGTAC82500.024.37480710-11
GAGTACT59550.023.67800112-13
TAAGGTG19650.023.6175985
GTAAGGT18800.023.441744
GGACCGA3850.023.1797646
Produced by FastQC (version 0.11.5)