FastQCFastQC Report
Thu 26 May 2016
SRR1364452_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364452_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143788
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16300.050.3898281
GTATCAA31300.046.397741
TCAACGC32350.042.288394
ATCAACG32450.041.788563
CAACGCA33250.041.1456645
GTACATG93300.040.5082471
AACGCAG35350.038.3648346
TACATGG98100.037.407042
ACATGGG97750.035.7764973
TATCAAC39700.035.6552662
CATGGGG53900.032.2226834
ACGCAGA44650.030.3739517
CGCAGAG46300.029.419988
CATGGGA52800.028.1626634
GAGTACA66050.025.9520131
ATGGGGA32550.024.4873435
TATAGGG4700.024.0432382
AGTACAT69950.023.1269532
GCAGAGT60050.022.4864569
GTATAGG7200.022.3194051
Produced by FastQC (version 0.11.5)