FastQCFastQC Report
Thu 26 May 2016
SRR1364452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364452_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143788
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26720.12463919006916728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24390.11377057806089035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.074.806181
GTATCAA53400.052.2089771
ATCAACG66250.041.7032473
TCAACGC66600.041.4831124
CAACGCA67200.040.907835
AACGCAG69800.039.4665266
TATCAAC71350.038.6407052
GTACATG89250.036.71091
TACATGG95350.033.5360262
ACGCAGA83050.033.241587
ACATGGG96100.032.4672623
CGCAGAG85200.032.260828
CATGGGG51200.028.0268784
GCAGAGT100650.026.717619
CATGGGA51750.026.463374
GAGTACT52500.026.17734712-13
TGGTATC15050.025.3222052
CAGAGTA95350.025.2389210-11
GAGTACA65150.024.596791
AGAGTAC88800.024.08567810-11
Produced by FastQC (version 0.11.5)