FastQCFastQC Report
Thu 26 May 2016
SRR1364451_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364451_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530194
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27400.043.2249031
ATCAACG27950.040.215533
GGTATCA21400.038.379281
CAACGCA28900.038.283025
GTACATG85350.037.7943841
TCAACGC31100.035.953874
TACATGG90550.035.0068132
AACGCAG32150.034.4123576
ACATGGG90400.033.8807223
TATCAAC34400.033.539542
CATGGGG53950.028.9942874
ACGCAGA39600.028.0885247
CGCAGAG41400.026.5799318
AGTACAT63750.025.4680822
CATGGGA45950.024.8521444
GAGTACA68850.024.3767991
TATTCCG4850.023.3025235
ATGGGGA34350.022.5116675
GTAAGGT7700.022.4003834
ATTCCGT4500.021.1490046
Produced by FastQC (version 0.11.5)