##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364451_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2530194 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.855555739994642 31.0 31.0 34.0 27.0 34.0 2 30.92606061037217 31.0 31.0 34.0 27.0 34.0 3 30.892102344721394 31.0 31.0 34.0 27.0 34.0 4 34.50997631011693 37.0 35.0 37.0 31.0 37.0 5 34.68970798286614 37.0 35.0 37.0 32.0 37.0 6 34.79756453457719 37.0 35.0 37.0 32.0 37.0 7 34.66550746701636 37.0 35.0 37.0 32.0 37.0 8 34.54830420117983 37.0 35.0 37.0 31.0 37.0 9 35.67221604351287 39.0 35.0 39.0 30.0 39.0 10-11 35.90594476154793 39.0 36.0 39.0 30.5 39.0 12-13 35.977092270395076 39.0 36.0 39.0 30.5 39.0 14-15 37.14941245611996 40.0 37.0 41.0 31.0 41.0 16-17 37.29581249501027 40.0 37.0 41.0 31.0 41.0 18-19 37.26522669803185 40.0 37.0 41.0 31.0 41.0 20-21 37.2020892469115 40.0 37.0 41.0 31.0 41.0 22-23 37.09675305529932 40.0 37.0 41.0 30.0 41.0 24-25 37.143925525078316 40.0 37.0 41.0 30.5 41.0 26-27 37.149731403995105 40.0 37.0 41.0 30.0 41.0 28-29 37.09369459416946 40.0 37.0 41.0 30.0 41.0 30-31 36.867470439025624 40.0 36.0 41.0 30.0 41.0 32-33 36.947351665524465 40.0 36.0 41.0 30.0 41.0 34-35 36.902998940002234 40.0 36.0 41.0 30.0 41.0 36-37 36.7939486063124 40.0 36.0 41.0 30.0 41.0 38-39 36.65592855725687 40.0 36.0 41.0 30.0 41.0 40-41 36.63042241029739 40.0 36.0 41.0 30.0 41.0 42-43 36.504854173237305 40.0 36.0 41.0 30.0 41.0 44-45 36.49324992470933 40.0 35.5 41.0 30.0 41.0 46-47 36.34757591710359 40.0 35.0 41.0 29.0 41.0 48-49 36.18110310908966 39.0 35.0 41.0 29.0 41.0 50-51 36.04853896578682 39.0 35.0 41.0 28.5 41.0 52-53 35.92883668208841 39.0 35.0 41.0 28.0 41.0 54-55 35.80879509634439 39.0 35.0 40.5 28.0 41.0 56-57 35.76419614464345 39.0 35.0 40.5 28.5 41.0 58-59 35.74066632835269 39.0 35.0 41.0 28.5 41.0 60-61 35.551690305170276 38.0 35.0 40.5 28.0 41.0 62-63 35.299780767798836 38.0 34.0 40.0 28.0 41.0 64-65 34.99389137749912 37.0 34.0 40.0 28.0 41.0 66-67 34.68391672733395 37.0 34.0 40.0 28.0 41.0 68-69 34.38637373260707 36.0 34.0 39.0 28.0 41.0 70-71 34.03935627070493 36.0 34.0 39.0 27.0 41.0 72-73 33.70421517085251 35.0 33.0 38.5 26.5 40.0 74-75 33.34248579357946 35.0 33.0 37.5 26.0 39.5 76-77 32.98987962978333 35.0 33.0 37.0 26.0 39.0 78-79 32.67079856327222 35.0 33.0 36.5 26.0 39.0 80-81 32.34941213993868 35.0 33.0 36.0 26.0 38.0 82-83 32.06346074648821 35.0 32.0 36.0 25.5 37.0 84-85 31.724191899909652 35.0 32.0 35.0 24.5 37.0 86-87 31.532688204936065 35.0 32.0 35.0 24.5 36.0 88-89 31.40108663604451 35.0 32.0 35.0 24.0 36.0 90-91 31.27990837856702 35.0 32.0 35.0 24.0 36.0 92-93 31.05979936716315 35.0 32.0 35.0 23.0 35.5 94-95 30.98424073410972 35.0 32.0 35.0 23.0 35.0 96-97 30.912322730984265 35.0 32.0 35.0 23.0 35.0 98-99 30.761258622856587 35.0 31.5 35.0 21.5 35.0 100-101 30.628680646622357 34.0 31.0 35.0 20.0 35.0 102-103 29.579290165101966 33.5 29.5 34.5 19.0 35.0 104-105 30.55086586245956 34.0 31.5 35.0 20.0 35.0 106-107 30.803423176246564 35.0 32.0 35.0 23.0 35.0 108-109 30.78882607420617 35.0 32.0 35.0 20.0 35.0 110-111 30.681736064507305 35.0 32.0 35.0 19.5 35.0 112-113 30.561218625923544 35.0 32.0 35.0 18.5 35.0 114-115 30.45996552043045 35.0 32.0 35.0 18.0 35.0 116-117 30.337347650022092 35.0 32.0 35.0 13.0 35.0 118-119 30.065742587327293 35.0 32.0 35.0 2.0 35.0 120-121 29.937517834600825 35.0 31.5 35.0 2.0 35.0 122-123 29.701709829364866 35.0 31.0 35.0 2.0 35.0 124-125 28.509325964728397 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6542082595835303 1101 2 0.6270027669072498 1101 3 0.8289815152988034 1101 4 0.7909750961375082 1101 5 0.6659421354197761 1101 6 0.6301358355262607 1101 7 0.744306859742224 1101 8 0.7663615273232551 1101 9 1.3181474818947834 1101 10-11 1.0068933191752265 1101 12-13 0.9079099764472716 1101 14-15 1.150713637776441 1101 16-17 0.9559947463757155 1101 18-19 0.9667580739314943 1101 20-21 1.105381858316747 1101 22-23 1.0238735604806593 1101 24-25 1.084483158762005 1101 26-27 1.0274686342476542 1101 28-29 1.0168620030854356 1101 30-31 1.175440948641409 1101 32-33 1.0548696913285482 1101 34-35 1.020081846311065 1101 36-37 0.9691328748380599 1101 38-39 1.1039121195543586 1101 40-41 0.9718544199823995 1101 42-43 0.9603653842903199 1101 44-45 0.89742865153449 1101 46-47 0.8977742239769242 1101 48-49 0.9057717111006767 1101 50-51 0.9277604850738541 1101 52-53 0.7979542445071814 1101 54-55 0.7910187431284683 1101 56-57 0.8378475805927721 1101 58-59 0.9731015178692246 1101 60-61 0.9048589109420817 1101 62-63 0.9215414421400681 1101 64-65 0.8010302921191084 1101 66-67 0.7900150409407587 1101 68-69 0.7521256167748831 1101 70-71 0.6811992610073645 1101 72-73 0.7319404255505759 1101 74-75 0.7358685768946387 1101 76-77 0.7922319459358675 1101 78-79 0.6704277619777628 1101 80-81 0.6781534693076878 1101 82-83 0.6866014694218734 1101 84-85 0.7790032317917834 1101 86-87 0.8865202216840871 1101 88-89 0.7792901656740057 1101 90-91 0.7369141327540341 1101 92-93 0.859376294759997 1101 94-95 0.8201563070983617 1101 96-97 0.7622967372045153 1101 98-99 0.7754366260621275 1101 100-101 0.8691851406025961 1101 102-103 0.8408823429933037 1101 104-105 0.7939393196807387 1101 106-107 0.6698508697821524 1101 108-109 0.6970527647523994 1101 110-111 0.7271891807025241 1101 112-113 0.7004137882541528 1101 114-115 0.7252026417626141 1101 116-117 0.680017070967498 1101 118-119 0.7388262518834949 1101 120-121 0.6283448281824029 1101 122-123 0.613604152568854 1101 124-125 0.2714341334810477 1102 1 0.289441075274091 1102 2 0.170577174129388 1102 3 0.3217448178456941 1102 4 0.22765164882392241 1102 5 0.2829657542389157 1102 6 0.22206912030034687 1102 7 0.2709727642281834 1102 8 0.35376631241880574 1102 9 0.6033944321671072 1102 10-11 0.4147397909707351 1102 12-13 0.38390414963527064 1102 14-15 0.5290391907631076 1102 16-17 0.36542780450524504 1102 18-19 0.32819827244383504 1102 20-21 0.5484756153272841 1102 22-23 0.5670956452026985 1102 24-25 0.5000471196424812 1102 26-27 0.4279631883027122 1102 28-29 0.38000899514371866 1102 30-31 0.4876995014775787 1102 32-33 0.37857974885384493 1102 34-35 0.24952209603243602 1102 36-37 0.18861431060192757 1102 38-39 0.336686761027984 1102 40-41 0.2678251188976475 1102 42-43 0.34987850712184354 1102 44-45 0.3305657903229786 1102 46-47 0.33717348862140284 1102 48-49 0.3000747749927086 1102 50-51 0.22323427789200423 1102 52-53 0.28043804407330697 1102 54-55 0.0782518663708558 1102 56-57 0.14916163224272339 1102 58-59 0.1865332930937953 1102 60-61 0.20203019334239514 1102 62-63 0.2513056261072677 1102 64-65 0.22900495913768992 1102 66-67 0.13344570564421332 1102 68-69 0.19725627196596918 1102 70-71 0.2984991331187956 1102 72-73 0.2735967648174551 1102 74-75 0.17982015936490114 1102 76-77 0.2704686349037644 1102 78-79 0.12553539246361822 1102 80-81 0.22290866117998576 1102 82-83 0.11153024199682449 1102 84-85 0.22489937990611608 1102 86-87 0.1625058203390779 1102 88-89 0.18190360443836795 1102 90-91 0.11781048257830662 1102 92-93 0.18124870779286795 1102 94-95 0.22156972061548785 1102 96-97 0.21075839750503889 1102 98-99 0.22881528235241788 1102 100-101 0.07804829745685282 1102 102-103 -0.035666264560592964 1102 104-105 0.11107359612338641 1102 106-107 0.039917070833812573 1102 108-109 0.06173461310612538 1102 110-111 0.06401079334888848 1102 112-113 0.042806242248286 1102 114-115 -0.12482315626812834 1102 116-117 -0.1889916787777608 1102 118-119 -0.11518372672929544 1102 120-121 -0.21870448297444867 1102 122-123 -0.14970624686594292 1102 124-125 -0.3507403829770688 1103 1 0.543872851975177 1103 2 0.6009029764218035 1103 3 0.7664143139667559 1103 4 0.6584460966141208 1103 5 0.589161386065129 1103 6 0.4945518908771902 1103 7 0.550210687851937 1103 8 0.7354906799815666 1103 9 1.2317138783468735 1103 10-11 0.8173642778132901 1103 12-13 0.675468047906918 1103 14-15 0.9242089789229553 1103 16-17 0.7547119268908915 1103 18-19 0.8207584531115728 1103 20-21 0.7513924507827099 1103 22-23 0.7797364424073123 1103 24-25 0.7568007595351034 1103 26-27 0.7995217890932693 1103 28-29 0.7307195723947828 1103 30-31 0.9647897331732054 1103 32-33 0.910742491623239 1103 34-35 0.8275289461539188 1103 36-37 0.8446004498204829 1103 38-39 0.9084934254494144 1103 40-41 0.9641127533864449 1103 42-43 0.89523952195551 1103 44-45 0.7865454662110949 1103 46-47 0.7290755840806895 1103 48-49 0.6190226946721751 1103 50-51 0.6385455546238532 1103 52-53 0.487404728883412 1103 54-55 0.40240647788146333 1103 56-57 0.3880166024573626 1103 58-59 0.5300439219938653 1103 60-61 0.5099652320438395 1103 62-63 0.492339718626134 1103 64-65 0.5289864266004827 1103 66-67 0.4161325762350856 1103 68-69 0.4164857752464215 1103 70-71 0.4865362938822173 1103 72-73 0.3910696689078321 1103 74-75 0.46243533526476455 1103 76-77 0.40723688610236763 1103 78-79 0.34449519400695294 1103 80-81 0.35361532440530397 1103 82-83 0.3810300090431227 1103 84-85 0.448234142809973 1103 86-87 0.469099339686629 1103 88-89 0.400786955662948 1103 90-91 0.46916061970611267 1103 92-93 0.4616718995771052 1103 94-95 0.4010400863535253 1103 96-97 0.40287087905356245 1103 98-99 0.35617002062211967 1103 100-101 0.3003129000081479 1103 102-103 0.26971885698244336 1103 104-105 0.35684137565017693 1103 106-107 0.31728682535839425 1103 108-109 0.3084510322855998 1103 110-111 0.29706854525989357 1103 112-113 0.25914735016832324 1103 114-115 0.25149838584648165 1103 116-117 0.23518462848306143 1103 118-119 0.17609674333424152 1103 120-121 -0.012268519015186996 1103 122-123 0.026124658575316317 1103 124-125 -0.15230605091867844 1104 1 0.32842645168622653 1104 2 0.45335682138524547 1104 3 0.590711895383901 1104 4 0.42917924360557436 1104 5 0.46003383943393317 1104 6 0.3634357878090526 1104 7 0.4076375207920009 1104 8 0.5764989294857301 1104 9 0.9796793751941877 1104 10-11 0.7428748525261355 1104 12-13 0.6450111096334652 1104 14-15 0.7177218978619138 1104 16-17 0.5985529765142132 1104 18-19 0.6182204668639386 1104 20-21 0.6587775179404005 1104 22-23 0.7392920817751047 1104 24-25 0.8041229733277149 1104 26-27 0.7714414501357112 1104 28-29 0.6779980025239851 1104 30-31 0.7478050978130142 1104 32-33 0.7275246275401486 1104 34-35 0.6093271946331811 1104 36-37 0.569615235517162 1104 38-39 0.7633939874655695 1104 40-41 0.6226804585214651 1104 42-43 0.6488478271473852 1104 44-45 0.5169922715279682 1104 46-47 0.5267859859235529 1104 48-49 0.6147213417047155 1104 50-51 0.6520731968358291 1104 52-53 0.546051029759056 1104 54-55 0.48156853173014724 1104 56-57 0.5073169958425439 1104 58-59 0.5716369505197321 1104 60-61 0.6449655383552724 1104 62-63 0.6316561853774019 1104 64-65 0.5870606073915852 1104 66-67 0.5759229140399782 1104 68-69 0.5923070891221514 1104 70-71 0.6560282843462062 1104 72-73 0.5531174929787994 1104 74-75 0.5765538391758227 1104 76-77 0.5263865190948138 1104 78-79 0.42504239979336234 1104 80-81 0.5457493918408574 1104 82-83 0.5564836040215866 1104 84-85 0.5619618455874189 1104 86-87 0.5521305715492844 1104 88-89 0.4564777866728136 1104 90-91 0.43332689949127 1104 92-93 0.4653818246774257 1104 94-95 0.4737408560807843 1104 96-97 0.3924744365633188 1104 98-99 0.4475249391853744 1104 100-101 0.3298074977486465 1104 102-103 0.3573078143244395 1104 104-105 0.3959278765486438 1104 106-107 0.3240557274598075 1104 108-109 0.21283693301688444 1104 110-111 0.33724325020649104 1104 112-113 0.3538693718122019 1104 114-115 0.33657958160504364 1104 116-117 0.24946367733232933 1104 118-119 0.11652526577787015 1104 120-121 0.03392789136316665 1104 122-123 0.11875294717701834 1104 124-125 -0.17237300682463896 1105 1 0.28524822180939324 1105 2 0.33342170976350616 1105 3 0.4254904041796017 1105 4 0.22446833289212975 1105 5 0.28089350205772234 1105 6 0.36501644847101744 1105 7 0.3587255572682295 1105 8 0.5393543071642739 1105 9 0.9346115784003146 1105 10-11 0.562395018143242 1105 12-13 0.6355614685789206 1105 14-15 0.3167119008606534 1105 16-17 0.44524334405846133 1105 18-19 0.3474000046245749 1105 20-21 0.6926713987061959 1105 22-23 0.736527280468664 1105 24-25 0.712161942484066 1105 26-27 0.6349066918593707 1105 28-29 0.6418044863036982 1105 30-31 0.7672887727497013 1105 32-33 0.6367820602581986 1105 34-35 0.5983872163830739 1105 36-37 0.6557648545327766 1105 38-39 0.7576512214720026 1105 40-41 0.6798208898340832 1105 42-43 0.6948576976958023 1105 44-45 0.6236697047518263 1105 46-47 0.637910043398179 1105 48-49 0.5406299054532084 1105 50-51 0.6198421710864181 1105 52-53 0.43908817438137504 1105 54-55 0.5203687831906763 1105 56-57 0.49505197234037723 1105 58-59 0.6564624275179582 1105 60-61 0.6233701007742098 1105 62-63 0.5903503609590004 1105 64-65 0.5675055769164459 1105 66-67 0.47602606343861 1105 68-69 0.5582379808315423 1105 70-71 0.6234549326591434 1105 72-73 0.5889497153334986 1105 74-75 0.6288005450790095 1105 76-77 0.585062449336192 1105 78-79 0.504902795843698 1105 80-81 0.5327193285782954 1105 82-83 0.578626401809025 1105 84-85 0.6809205605373947 1105 86-87 0.5974467578757583 1105 88-89 0.5966625162262957 1105 90-91 0.5163531330388516 1105 92-93 0.4926224789083591 1105 94-95 0.472272196517153 1105 96-97 0.5016411845211053 1105 98-99 0.4696930275434035 1105 100-101 0.35441883587273537 1105 102-103 0.35587528414736624 1105 104-105 0.29055832711822305 1105 106-107 0.26981377618389857 1105 108-109 0.22757320655970048 1105 110-111 0.3056038431619825 1105 112-113 0.3284397030825481 1105 114-115 0.21594807605737287 1105 116-117 0.2651844766588809 1105 118-119 0.04082065241649957 1105 120-121 0.05314872501262968 1105 122-123 0.021781673652270683 1105 124-125 -0.13544245111854636 1106 1 0.32222963032241836 1106 2 0.37151717131589734 1106 3 0.461570549463584 1106 4 0.3694069308270329 1106 5 0.3343698735986038 1106 6 0.290976345536734 1106 7 0.28522949844384726 1106 8 0.5308323017768473 1106 9 0.8598507488730078 1106 10-11 0.40431994573587104 1106 12-13 0.5018028553923841 1106 14-15 0.709017368578337 1106 16-17 0.6218787659414744 1106 18-19 0.6015724970349297 1106 20-21 0.7650774760426842 1106 22-23 0.6368275460265522 1106 24-25 0.6121078626333158 1106 26-27 0.6192967302399666 1106 28-29 0.5363211221123478 1106 30-31 0.5632829149782168 1106 32-33 0.5920007590934802 1106 34-35 0.5644414604072097 1106 36-37 0.5644959443344106 1106 38-39 0.6403536615294385 1106 40-41 0.6108077855789773 1106 42-43 0.5542302064423623 1106 44-45 0.37775412638750083 1106 46-47 0.5364642424571997 1106 48-49 0.46473494072140653 1106 50-51 0.478574146667043 1106 52-53 0.380925186717775 1106 54-55 0.20894474266175678 1106 56-57 0.28816290392159516 1106 58-59 0.33315295702583114 1106 60-61 0.26988778572385286 1106 62-63 0.38098912391925666 1106 64-65 0.43379737199289536 1106 66-67 0.37306081342782704 1106 68-69 0.4084178615853986 1106 70-71 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2216 88-89 -1.9557974823843693 2216 90-91 -1.7008091417724316 2216 92-93 -1.580399681050892 2216 94-95 -1.6026666725607903 2216 96-97 -1.5271321447985855 2216 98-99 -1.4961110524825294 2216 100-101 -1.6363759818922823 2216 102-103 -1.9401450696709972 2216 104-105 -1.7338673548157395 2216 106-107 -1.469840394149081 2216 108-109 -1.5188323994237187 2216 110-111 -1.4987023556061345 2216 112-113 -1.7527847099216665 2216 114-115 -1.5835750332836156 2216 116-117 -1.4754249801095156 2216 118-119 -1.2712210393354546 2216 120-121 -1.2169389191313051 2216 122-123 -1.1667195767529535 2216 124-125 -1.1604383988793927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31172.0 3 6471.0 4 4282.0 5 3742.0 6 4027.0 7 4671.0 8 5583.0 9 6178.0 10 6603.0 11 7124.0 12 7878.0 13 8343.0 14 8407.0 15 8197.0 16 7798.0 17 7422.0 18 7206.0 19 7024.0 20 7302.0 21 7917.0 22 9355.0 23 12322.0 24 16861.0 25 23374.0 26 28614.0 27 33541.0 28 40320.0 29 48731.0 30 59333.0 31 72156.0 32 91782.0 33 119666.0 34 163992.0 35 240580.0 36 431810.0 37 716955.0 38 263076.0 39 379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.772804230460075 18.012090419438344 12.23092843535004 24.984176914751547 2 15.945336868248525 19.592812866079516 39.36296433308513 25.09888593258683 3 18.969548555998912 24.016754459740604 29.11802740661578 27.8956695776447 4 12.512840173191655 15.701115943947878 37.233755377656216 34.552288505204245 5 13.674965051769227 38.05932484405204 34.69054067887111 13.575169425307617 6 33.469647314744996 36.02127588938055 17.275238045552346 13.23383875032211 7 29.565914708516424 31.71598699546359 20.88796353165014 17.830134764369845 8 26.62105168718379 35.27614624038056 19.897888001682105 18.204914070753546 9 26.779517662774982 15.61485744097258 19.485530673707007 38.12009422254543 10-11 25.138499884978692 26.067287070261024 28.107452827429302 20.686760217330985 12-13 25.589635528577727 23.291129534747732 27.915867222550816 23.203367714123722 14-15 22.80034900116696 25.104962199774512 24.893822422435676 27.20086637662285 16-17 23.2426019974108 28.33752713057119 25.646889847954185 22.772981024063828 18-19 23.273037026642697 27.311608216635854 26.738238267419145 22.677116489302303 20-21 23.668096171170546 26.796153501034283 26.779037394936555 22.756712932858612 22-23 23.690251335851045 26.513913464006972 26.499960572210856 23.29587462793112 24-25 23.441792019959294 26.747213728969687 26.498019031605175 23.31297521946584 26-27 23.352933178178876 27.06065174359039 26.344472148088183 23.24194293014255 28-29 23.28796496907996 26.986788701235792 26.517597772484397 23.20764855719985 30-31 23.433897226389323 26.672438022164375 26.517876378355837 23.37578837309046 32-33 23.197962170114923 26.770646317351694 26.47311366849891 23.558277844034475 34-35 23.718486967344205 26.761669749800753 26.22018300398373 23.299660278871308 36-37 23.776436454294984 26.529531007983252 26.201055185598126 23.49297735212363 38-39 23.32121631302028 26.705095966208038 26.48918311389966 23.48450460687202 40-41 23.574023136559212 26.412726668739005 26.575602291009055 23.437647903692724 42-43 23.320437424077532 26.385536240126743 26.5651910049699 23.72883533082582 44-45 23.360359881454443 26.64864150244658 26.509638792545864 23.481359823553113 46-47 23.57948908877195 26.891531160652388 26.127978385437046 23.40100136513861 48-49 23.51047987770594 26.563345181311615 26.39909405950867 23.527080881473776 50-51 23.412666887213316 26.739146674192128 26.434531439161734 23.413654999432822 52-53 23.521960052007607 26.55974896586561 26.338426123644748 23.579864858482036 54-55 23.362335338276026 26.52799064030696 26.3801446444117 23.729529377005314 56-57 23.28237764993566 26.900541454779525 26.4311040938266 23.385976801458213 58-59 23.459458861227926 26.449226169619163 26.350272798653112 23.741042170499803 60-61 23.281949039170552 26.50831141732151 26.744247405109284 23.465492138398652 62-63 23.192807695097436 26.555509461847947 26.74289059459763 23.508792248456984 64-65 23.272037076965614 26.426725423377768 26.49291170426017 23.808325795396446 66-67 23.10455621964864 26.725107560746203 26.360896171886907 23.80944004771825 68-69 23.04471686277176 26.374596764789704 26.873222968467314 23.70746340397122 70-71 23.062870639868084 26.20770693779337 26.832923333770736 23.89649908856781 72-73 23.14178966720385 26.38970744311855 26.447674917298023 24.02082797237958 74-75 23.123131076164142 26.606973099355102 26.39332533554162 23.876570488939134 76-77 23.27314609321796 26.395595500324305 26.552489694863617 23.77876871159412 78-79 23.341261567497863 26.30748175633279 26.698353252733465 23.652903423435887 80-81 23.26369742670877 26.588627176146783 26.56514773666504 23.5825276604794 82-83 23.301719053635043 26.520497564199662 26.5713125809364 23.60647080122889 84-85 23.36162538477882 26.26103197308154 26.630380109590934 23.746962532548704 86-87 23.20931850478594 26.247576394228012 26.826929348309452 23.71617575267659 88-89 23.357331704234817 26.24634633746333 26.72015390790942 23.67616805039243 90-91 23.33245187974918 26.448093846742495 26.724901893820814 23.494552379687512 92-93 23.309006818499377 26.055675682833158 26.771617129234095 23.863700369433374 94-95 23.23631910899851 26.12686447112597 26.80654638504953 23.83027003482599 96-97 23.31383642677751 26.036730021731447 26.81450481309294 23.834928738398105 98-99 23.271768321037392 26.092776168555737 26.582611192609747 24.052844317797124 100-101 23.097054452314698 25.949753351884645 26.926147862382997 24.027044333417656 102-103 23.113885664119923 25.69310133969881 27.00746314968554 24.18554984649573 104-105 22.93323035347218 25.919619975853227 26.959385867950857 24.187763802723737 106-107 23.07380214397908 25.90412804113101 26.795568646143845 24.22650116874606 108-109 23.083771374841337 25.95321580336638 26.675379079892487 24.287633741899796 110-111 23.04794048140874 26.03545092099589 26.84951144906015 24.067097148535215 112-113 23.059235067713455 26.007855847665727 27.074551500269273 23.85835758435155 114-115 22.923277267859074 26.20048188256351 26.73671507561951 24.139525773957907 116-117 22.821425543365155 26.05923422831406 27.037768802036283 24.081571426284498 118-119 22.845950313708094 26.18448570885354 26.739871823309464 24.229692154128905 120-121 22.86219120385872 26.34630156222018 26.850622306576565 23.940884927344527 122-123 22.873122453860645 26.41129996659505 26.837677624413686 23.877899955130623 124-125 22.823474695638343 26.467435882656233 26.77087152524197 23.938217896463456 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 283.0 1 240.0 2 173.5 3 140.5 4 129.0 5 107.0 6 86.0 7 97.5 8 91.5 9 88.5 10 104.5 11 97.0 12 104.0 13 112.0 14 108.5 15 129.5 16 166.0 17 210.0 18 259.5 19 332.0 20 435.0 21 633.0 22 882.5 23 1292.0 24 1939.0 25 2895.0 26 4502.5 27 6949.0 28 9980.0 29 13985.5 30 19083.5 31 24963.5 32 31227.0 33 36450.5 34 41314.0 35 47252.0 36 55763.0 37 63836.5 38 68897.5 39 73493.5 40 77152.5 41 81429.0 42 85646.5 43 89571.5 44 94748.0 45 99358.0 46 100539.0 47 99759.0 48 98175.5 49 96451.5 50 96681.0 51 95451.5 52 95270.0 53 97135.0 54 99467.0 55 94764.5 56 83874.5 57 76389.5 58 70770.5 59 61784.5 60 50127.5 61 40735.5 62 33110.0 63 25805.0 64 19922.0 65 15418.0 66 10966.0 67 8524.0 68 6547.5 69 4323.0 70 3107.5 71 2204.5 72 1626.0 73 1268.0 74 1042.5 75 836.5 76 595.5 77 359.5 78 184.0 79 111.5 80 65.5 81 35.5 82 24.0 83 22.0 84 18.5 85 20.0 86 17.5 87 8.5 88 8.0 89 7.5 90 7.0 91 6.0 92 3.0 93 3.0 94 5.5 95 5.5 96 3.5 97 2.0 98 1.0 99 2.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08860980620458353 2 2.3713596664919764E-4 3 7.904532221639922E-5 4 0.0019366103943017807 5 9.09021205488591E-4 6 2.3713596664919764E-4 7 0.0 8 0.001225202494354188 9 5.533172555147944E-4 10-11 0.00901116673266951 12-13 0.0019958943859640804 14-15 0.004604390019105255 16-17 0.002707302285911673 18-19 0.012904148851827172 20-21 0.01632285903768644 22-23 0.009979471929820401 24-25 0.009663290640954805 26-27 0.009564483988184306 28-29 0.007924293552194022 30-31 0.01814090144866362 32-33 0.00203541704707228 34-35 0.001205441163800088 36-37 9.09021205488591E-4 38-39 2.5689729720329747E-4 40-41 0.0016797130970984833 42-43 0.002509688980370675 44-45 0.0010868731804754893 46-47 0.0018970877331935811 48-49 0.008951882741007212 50-51 0.004624151349659354 52-53 0.0070943176689218295 54-55 0.007766202907761223 56-57 0.009900426607604002 58-59 0.008477610807708817 60-61 0.010690879829767994 62-63 0.012785580868502573 64-65 0.008438088146600615 66-67 0.013615556751774765 68-69 0.013556272760112465 70-71 0.004347492721901957 72-73 0.012923910182381272 74-75 0.013002955504597673 76-77 0.005730785860688943 78-79 0.00816142951884322 80-81 0.012686774215732076 82-83 0.016994744276525833 84-85 0.01310176215736817 86-87 0.015374315171089647 88-89 0.014603623279479755 90-91 0.01114539043251229 92-93 0.009426154674305605 94-95 0.007390737627233327 96-97 0.008694985443803914 98-99 0.006264341785649638 100-101 0.010829209143646693 102-103 0.011738230349135284 104-105 0.009860903946495803 106-107 0.005216991266282348 108-109 0.003458232846967466 110-111 0.006363148438420137 112-113 0.00914949604654821 114-115 0.012825103529610775 116-117 0.011402287729715587 118-119 0.21950885979494061 120-121 0.025808297703654344 122-123 0.024859753837057554 124-125 0.2345867550077188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2530194.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.45993223797704 #Duplication Level Percentage of deduplicated Percentage of total 1 84.84765935712224 58.9351266949667 2 9.078207081018382 12.611432973797204 3 2.785208056881196 5.803810886989067 4 1.1827616057947221 3.2861816396872925 5 0.5972410793693619 2.0742162451366073 6 0.34978868541304126 1.4577779031840548 7 0.23582195247212698 1.1466123787258968 8 0.15709320108298977 0.8729346481817097 9 0.11364940952462868 0.710467225602013 >10 0.5755328407488826 7.371661745122404 >50 0.049933821408126335 2.397730782870046 >100 0.02669838866384908 3.1650512945549236 >500 4.0452050041566443E-4 0.1669955811820955 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.952266110819961E-5 0.0 0.0 0.0 0.0 6 7.904532221639922E-5 0.0 0.0 0.0 0.0 7 1.1856798332459882E-4 0.0 0.0 0.0 0.0 8 1.1856798332459882E-4 0.0 0.0 0.0 0.0 9 1.1856798332459882E-4 0.0 0.0 0.0 0.0 10-11 1.1856798332459882E-4 0.0 0.0 0.0 0.0 12-13 1.1856798332459882E-4 0.0 0.0 0.0 0.0 14-15 1.1856798332459882E-4 0.0 0.0 0.0 0.0 16-17 1.1856798332459882E-4 0.0 0.0 0.0 0.0 18-19 1.5809064443279844E-4 0.0 0.0 0.0 0.0 20-21 1.9761330554099804E-4 0.0 0.0 0.0 0.0 22-23 1.9761330554099804E-4 0.0 0.0 1.9761330554099805E-5 0.0 24-25 2.7665862775739727E-4 0.0 0.0 1.7785197498689824E-4 0.0 26-27 2.7665862775739727E-4 0.0 0.0 6.916465693934932E-4 0.0 28-29 3.359426194196967E-4 0.0 0.0 0.003161812888655969 0.0 30-31 3.952266110819961E-4 0.0 0.0 0.011639423696364785 0.0 32-33 3.952266110819961E-4 0.0 0.0 0.03266547940592698 0.0 34-35 4.149879416360959E-4 0.0 0.0 0.061497260684358596 0.0 36-37 4.347492721901957E-4 0.0 0.0 0.111078439044595 0.0 38-39 4.742719332983953E-4 0.0 0.0 0.18514390596136104 0.0 40-41 4.742719332983953E-4 0.0 0.0 0.264129944186098 0.0 42-43 4.940332638524951E-4 0.0 0.0 0.34532925143289406 0.0 44-45 5.533172555147945E-4 0.0 0.0 0.4446892214589079 0.0 46-47 7.311692305016928E-4 0.0 0.0 0.5629805461557493 0.0 48-49 7.904532221639922E-4 0.0 0.0 0.692812487896185 0.0 50-51 7.904532221639922E-4 0.0 0.0 0.8278811822334572 0.0 52-53 7.904532221639922E-4 0.0 0.0 0.9603413809375883 0.0 54-55 7.904532221639922E-4 0.0 0.0 1.1063578524018316 0.0 56-57 7.904532221639922E-4 0.0 0.0 1.276166965853211 0.0 58-59 7.904532221639922E-4 0.0 0.0 1.46959086931674 0.0 60-61 7.904532221639922E-4 0.0 0.0 1.6768279428375847 0.0 62-63 7.904532221639922E-4 0.0 0.0 1.880033704925393 0.0 64-65 8.299758832721918E-4 0.0 0.0 2.0898595127488244 0.0 66-67 9.09021205488591E-4 0.0 0.0 2.332825071911482 0.0 68-69 9.485438665967906E-4 0.0 0.0 2.604108617758164 0.0 70-71 9.880665277049903E-4 0.0 0.0 2.8950349261756214 0.0 72-73 9.880665277049903E-4 0.0 0.0 3.1825622857377738 0.0 74-75 9.880665277049903E-4 0.0 0.0 3.4793577093297987 0.0 76-77 9.880665277049903E-4 0.0 0.0 3.806882001933449 0.0 78-79 9.880665277049903E-4 0.0 0.0 4.1710042787232915 0.0 80-81 0.001225202494354188 0.0 0.0 4.565163777955366 0.0 82-83 0.0012647251554623875 0.0 0.0 4.968947045167288 0.0 84-85 0.0012844864860164873 0.0 0.0 5.3690744662267 0.0 86-87 0.001304247816570587 0.0 0.0 5.790563885615095 0.0 88-89 0.001304247816570587 0.0 0.0 6.252168806028313 0.0 90-91 0.0014820997915574853 0.0 0.0 6.757821732246619 0.0 92-93 0.0015018611221115851 0.0 0.0 7.278947780288784 0.0 94-95 0.001521622452665685 0.0 0.0 7.807859792569266 0.0 96-97 0.0015413837832197847 0.0 0.0 8.33853056326906 0.0 98-99 0.0015413837832197847 0.0 0.0 8.90406032106629 0.0 100-101 0.0016797130970984833 0.0 0.0 9.52363731792898 0.0 102-103 0.0018378037415312817 0.0 0.0 10.165406289003926 0.0 104-105 0.00203541704707228 0.0 0.0 10.814565997706104 0.0 106-107 0.0020947010387345794 0.0 0.0 11.457421841961526 0.0 108-109 0.0020947010387345794 0.0 0.0 12.124465554815163 0.0 110-111 0.0020947010387345794 0.0 0.0 12.837059134596004 0.0 112-113 0.0020947010387345794 0.0 0.0 13.580045640769047 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2740 0.0 43.224903 1 ATCAACG 2795 0.0 40.21553 3 GGTATCA 2140 0.0 38.37928 1 CAACGCA 2890 0.0 38.28302 5 GTACATG 8535 0.0 37.794384 1 TCAACGC 3110 0.0 35.95387 4 TACATGG 9055 0.0 35.006813 2 AACGCAG 3215 0.0 34.412357 6 ACATGGG 9040 0.0 33.880722 3 TATCAAC 3440 0.0 33.53954 2 CATGGGG 5395 0.0 28.994287 4 ACGCAGA 3960 0.0 28.088524 7 CGCAGAG 4140 0.0 26.579931 8 AGTACAT 6375 0.0 25.468082 2 CATGGGA 4595 0.0 24.852144 4 GAGTACA 6885 0.0 24.376799 1 TATTCCG 485 0.0 23.302523 5 ATGGGGA 3435 0.0 22.511667 5 GTAAGGT 770 0.0 22.400383 4 ATTCCGT 450 0.0 21.149004 6 >>END_MODULE