FastQCFastQC Report
Thu 26 May 2016
SRR1364451_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364451_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530194
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28200.056.814991
GTATCAA44550.051.4722061
ATCAACG52350.042.42263
CAACGCA55050.040.3180055
TCAACGC55450.040.050914
AACGCAG56700.039.141646
TATCAAC58200.038.160742
GTACATG88050.034.4308851
ACGCAGA69900.031.7500827
TACATGG95550.031.4696252
ACATGGG92700.031.4075663
CGCAGAG71800.030.658878
CATGGGG54250.026.2303034
GCAGAGT86300.025.4386739
CATGGGA47200.023.8410264
GAGTACA73000.023.4163281
AGTACAT68600.023.0881142
ATGGGGA31600.022.9731375
GAGTACT42800.021.96302612-13
CGTATAC1653.2010756E-421.6520082
Produced by FastQC (version 0.11.5)