FastQCFastQC Report
Thu 26 May 2016
SRR1364450_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364450_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810685
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15350.055.06551
GTATCAA29600.052.08441
TCAACGC32950.045.1269764
ATCAACG33500.044.563633
CAACGCA34400.042.8825765
GTACATG98900.041.4087141
AACGCAG35900.040.5926366
TATCAAC38000.039.7559132
TACATGG102800.039.0537152
ACATGGG100500.038.172033
CATGGGA70200.031.772094
CATGGGG36550.031.2439174
ACGCAGA47300.030.8092127
CGCAGAG47950.030.143478
ATGGGAG31700.026.645065
ATGGGAT29400.025.2900855
GCAGAGT56750.024.8403749
GAGTACA69250.024.325751
GGACCGA2752.1263986E-923.7922576
CGGTTAC3050.023.40997928-29
Produced by FastQC (version 0.11.5)