FastQCFastQC Report
Thu 26 May 2016
SRR1364450_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364450_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810685
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.13851111595887744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22860.12625056263237394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28150.067.95331
GTATCAA48400.052.8196831
ATCAACG60750.041.8602033
TCAACGC61500.041.2528724
CAACGCA61850.041.0805135
AACGCAG63300.040.1372766
TATCAAC63950.039.3064352
GTACATG93800.037.609951
TACATGG97700.035.3000832
ACATGGG98450.034.6019063
ACGCAGA78200.032.4135487
CGCAGAG78500.032.0587438
CATGGGA68650.030.7969364
GTGGTAT15300.028.4325181
GCAGAGT89400.027.6841749
CATGGGG38450.027.105754
ATGGGAG30550.026.48965
GAGTACA71950.026.006551
TGGTATC14450.025.969552
AGTACAT72950.024.7405362
Produced by FastQC (version 0.11.5)