##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364450_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1810685 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.945246688407977 33.0 31.0 34.0 30.0 34.0 2 31.87976981087268 33.0 31.0 34.0 30.0 34.0 3 32.11340790916145 33.0 31.0 34.0 30.0 34.0 4 35.79622960371351 37.0 35.0 37.0 35.0 37.0 5 35.73024076523526 37.0 35.0 37.0 33.0 37.0 6 35.79306836915311 37.0 35.0 37.0 33.0 37.0 7 35.70920728895418 37.0 35.0 37.0 33.0 37.0 8 35.71970110759188 37.0 35.0 37.0 33.0 37.0 9 37.36681090305603 39.0 37.0 39.0 34.0 39.0 10-11 37.321555654351805 39.0 37.0 39.0 34.0 39.0 12-13 37.28734125482897 39.0 37.0 39.0 33.0 39.0 14-15 38.59937344154284 40.0 38.0 41.0 34.0 41.0 16-17 38.61417585057589 40.0 38.0 41.0 34.0 41.0 18-19 38.66446593416304 40.0 38.0 41.0 34.0 41.0 20-21 38.665883353537474 40.0 38.0 41.0 34.0 41.0 22-23 38.63492518024947 40.0 38.0 41.0 34.0 41.0 24-25 38.583944474052636 40.0 38.0 41.0 34.0 41.0 26-27 38.48007853381455 40.0 38.0 41.0 34.0 41.0 28-29 38.40426634119132 40.0 38.0 41.0 34.0 41.0 30-31 38.28920215277644 40.0 38.0 41.0 33.5 41.0 32-33 38.16666482574274 40.0 37.5 41.0 33.0 41.0 34-35 38.06525430983302 40.0 37.0 41.0 33.0 41.0 36-37 37.974317454444034 40.0 37.0 41.0 33.0 41.0 38-39 37.881977815025806 40.0 37.0 41.0 33.0 41.0 40-41 37.766937374529526 40.0 37.0 41.0 33.0 41.0 42-43 37.62354329991136 40.0 37.0 41.0 32.5 41.0 44-45 37.45249201269132 40.0 37.0 41.0 32.0 41.0 46-47 37.24193716742559 40.0 36.5 41.0 31.0 41.0 48-49 37.02411186926495 40.0 36.0 41.0 31.0 41.0 50-51 36.80564151136172 39.5 36.0 41.0 30.5 41.0 52-53 36.67652573473575 39.0 35.0 41.0 30.0 41.0 54-55 36.521346065163186 39.0 35.0 41.0 30.0 41.0 56-57 36.31004454115431 39.0 35.0 41.0 30.0 41.0 58-59 36.09014516605594 38.5 35.0 40.5 29.5 41.0 60-61 35.85121128191817 38.0 35.0 40.0 29.0 41.0 62-63 35.887935781209876 38.0 35.0 40.0 30.0 41.0 64-65 35.67377208073188 37.5 35.0 40.0 29.0 41.0 66-67 35.39597804145944 37.0 34.5 40.0 29.0 41.0 68-69 35.07589089212094 36.5 34.0 39.0 29.0 41.0 70-71 34.73506683934533 36.0 34.0 39.0 29.0 41.0 72-73 34.3888544942936 35.5 34.0 39.0 29.0 40.5 74-75 34.04637526681891 35.0 34.0 37.5 29.0 39.5 76-77 33.72467132604511 35.0 34.0 37.0 28.5 39.0 78-79 33.40077871081938 35.0 33.5 36.5 28.0 39.0 80-81 33.11474248695936 35.0 33.0 36.0 28.0 38.0 82-83 32.85775328121677 35.0 33.0 36.0 27.0 37.0 84-85 32.6417123906146 35.0 33.0 35.0 27.0 37.0 86-87 32.4545293631968 35.0 33.0 35.0 27.0 36.0 88-89 32.28139433418844 35.0 33.0 35.0 26.5 36.0 90-91 32.16202956339728 35.0 33.0 35.0 26.0 36.0 92-93 32.04861088483088 35.0 33.0 35.0 26.0 36.0 94-95 31.937972093434254 35.0 33.0 35.0 26.0 35.0 96-97 31.88970279203727 35.0 33.0 35.0 26.0 35.0 98-99 31.81056036803751 35.0 33.0 35.0 25.0 35.0 100-101 31.803500885024178 35.0 33.0 35.0 25.5 35.0 102-103 30.671953708127035 33.5 30.5 34.5 24.0 35.0 104-105 31.651101931037147 35.0 32.5 35.0 25.0 35.0 106-107 31.843154662462 35.0 33.0 35.0 25.5 35.0 108-109 31.84010388333697 35.0 33.0 35.0 25.0 35.0 110-111 31.78042646843598 35.0 33.0 35.0 25.0 35.0 112-113 31.723068341539253 35.0 33.0 35.0 25.0 35.0 114-115 31.61590585883243 35.0 33.0 35.0 25.0 35.0 116-117 31.52914090523752 35.0 33.0 35.0 25.0 35.0 118-119 31.417840209644417 35.0 33.0 35.0 25.0 35.0 120-121 31.28660451707503 35.0 33.0 35.0 24.5 35.0 122-123 31.0923045698175 35.0 33.0 35.0 24.0 35.0 124-125 30.058554083123237 34.5 31.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3838556834723512 1101 2 0.5234830020079251 1101 3 0.3398369155393013 1101 4 0.2386339313203365 1101 5 0.287708709802061 1101 6 0.35179673991010674 1101 7 0.3623621136990067 1101 8 0.3242839454692472 1101 9 0.4116957834396615 1101 10-11 0.47775011451054894 1101 12-13 0.43456085495328267 1101 14-15 0.4987229695040085 1101 16-17 0.5308923812371731 1101 18-19 0.41688664278537857 1101 20-21 0.4290775314974198 1101 22-23 0.4559119333476147 1101 24-25 0.5570368769657961 1101 26-27 0.5227289490512348 1101 28-29 0.44484047961682194 1101 30-31 0.458065725730215 1101 32-33 0.3061805187033926 1101 34-35 0.36471327974336987 1101 36-37 0.4442019096302161 1101 38-39 0.4359380654233078 1101 40-41 0.36860353088933806 1101 42-43 0.22748589852817247 1101 44-45 0.3910306074497143 1101 46-47 0.48121752833398546 1101 48-49 0.2691364619720886 1101 50-51 0.3564796911082482 1101 52-53 0.47786817972901474 1101 54-55 0.3956670707996537 1101 56-57 0.3898707329347104 1101 58-59 0.27343734621912574 1101 60-61 0.1534579084255867 1101 62-63 0.3686105109601314 1101 64-65 0.3979380520766469 1101 66-67 0.3727436888964988 1101 68-69 0.45056845338107365 1101 70-71 0.48070347375730194 1101 72-73 0.47153257929028314 1101 74-75 0.43186754596796817 1101 76-77 0.4444037714383313 1101 78-79 0.4461948468808714 1101 80-81 0.30111054480048693 1101 82-83 0.39540725441597147 1101 84-85 0.3658087581809397 1101 86-87 0.46279069954600516 1101 88-89 0.4824257797381364 1101 90-91 0.4790216608745155 1101 92-93 0.5535014246644963 1101 94-95 0.550580185851306 1101 96-97 0.3435261987694247 1101 98-99 0.5452926443932604 1101 100-101 0.5429090004745625 1101 102-103 0.6090123766641113 1101 104-105 0.5833810239698458 1101 106-107 0.5361126921844104 1101 108-109 0.539180024186205 1101 110-111 0.6136681587906132 1101 112-113 0.6592021254414355 1101 114-115 0.6941241815719046 1101 116-117 0.6397314784399804 1101 118-119 0.6383543084641659 1101 120-121 0.6254473230886148 1101 122-123 0.6768853873191354 1101 124-125 0.6018534937837217 1102 1 0.3096611755036811 1102 2 0.4114876509487537 1102 3 0.2443987163735457 1102 4 0.20545272436505968 1102 5 0.15579446086476878 1102 6 0.2172649736222283 1102 7 0.2534769305944309 1102 8 0.2094382982667966 1102 9 0.33501590570105577 1102 10-11 0.28195799205453653 1102 12-13 0.2655338703067329 1102 14-15 0.27598048145811305 1102 16-17 0.34184337936610376 1102 18-19 0.252937940375098 1102 20-21 0.32019179547410204 1102 22-23 0.2908474409049049 1102 24-25 0.24594239642190274 1102 26-27 0.2654342920156765 1102 28-29 0.22899427064892564 1102 30-31 0.25438511150383647 1102 32-33 0.22113276864035925 1102 34-35 0.2352293851606504 1102 36-37 0.14841277293542277 1102 38-39 0.11228707658342074 1102 40-41 0.014094699671097999 1102 42-43 0.1710837629347992 1102 44-45 0.05245938266834571 1102 46-47 -0.08886962271899534 1102 48-49 -0.1513126296929883 1102 50-51 -0.13406291017540894 1102 52-53 -0.10563250367854948 1102 54-55 -0.1680833549477896 1102 56-57 -0.1435266652508247 1102 58-59 -0.2280356293241752 1102 60-61 -0.1589815469715603 1102 62-63 -0.1328831990362005 1102 64-65 -0.02116689411329986 1102 66-67 -0.041526259306074564 1102 68-69 -0.11545059798728374 1102 70-71 -0.10614817371939722 1102 72-73 -0.14867693268151783 1102 74-75 -0.1431900681655165 1102 76-77 -0.007590164148844281 1102 78-79 -0.11442799397141812 1102 80-81 -0.19043422171299085 1102 82-83 -0.10213253361709462 1102 84-85 -0.23616134660822752 1102 86-87 -0.14637564274165982 1102 88-89 -0.10709950634202414 1102 90-91 -0.1067100496791582 1102 92-93 -0.0615490789339006 1102 94-95 -0.0663239778480289 1102 96-97 -0.009311988453287512 1102 98-99 -0.03965419799835601 1102 100-101 -0.11236357740957104 1102 102-103 -0.05316062718862824 1102 104-105 -0.01603210807157396 1102 106-107 -0.011891989931541502 1102 108-109 -0.11602896804196661 1102 110-111 -0.06291860743091604 1102 112-113 -0.045472636692917234 1102 114-115 -0.10556966731761364 1102 116-117 -0.07253181704422573 1102 118-119 -0.1031155375590167 1102 120-121 -0.18175239779799668 1102 122-123 -0.16075499875085342 1102 124-125 -0.29329956518395406 1103 1 0.2492687009087895 1103 2 0.4124360167823191 1103 3 0.2724904494139153 1103 4 0.1482077367243022 1103 5 0.09412239275161483 1103 6 0.03807519059558473 1103 7 0.17784221485699447 1103 8 0.1373575512192815 1103 9 0.19746578210863674 1103 10-11 0.20935719557004262 1103 12-13 0.2026842410839933 1103 14-15 0.2521896272971631 1103 16-17 0.2564327778829849 1103 18-19 0.19267647374504548 1103 20-21 0.20985644804207482 1103 22-23 0.12762030550254622 1103 24-25 0.21681180043178472 1103 26-27 0.2040912543886506 1103 28-29 0.26683222725425537 1103 30-31 0.2684173828578622 1103 32-33 0.23031348822289033 1103 34-35 0.1300021124333739 1103 36-37 0.21901356941990713 1103 38-39 0.19088828504263233 1103 40-41 0.1385962102451188 1103 42-43 0.0635004067139846 1103 44-45 0.10131917393447765 1103 46-47 0.12174408230709588 1103 48-49 0.16644938623669958 1103 50-51 0.11043736447441432 1103 52-53 0.08264297009238675 1103 54-55 0.03157443137465776 1103 56-57 0.07973589997850183 1103 58-59 -0.05397300916487069 1103 60-61 -0.0786714673231117 1103 62-63 -0.040094267424002794 1103 64-65 0.10573986227230847 1103 66-67 0.002327077689258772 1103 68-69 0.05133352725302842 1103 70-71 0.002130733174311672 1103 72-73 0.03695970560467288 1103 74-75 0.030731381985539485 1103 76-77 0.00526454609559579 1103 78-79 0.06971660915958466 1103 80-81 0.06598928831173367 1103 82-83 -0.040308172177930146 1103 84-85 -0.0034366830542538196 1103 86-87 0.10944569754947508 1103 88-89 0.016456659546470576 1103 90-91 -0.0765743726673449 1103 92-93 -0.004032050644966034 1103 94-95 0.02414347614812229 1103 96-97 -0.041397267222855305 1103 98-99 -0.025971830516894556 1103 100-101 -0.062117353844616474 1103 102-103 -0.053888861190277026 1103 104-105 0.1124910998383406 1103 106-107 0.11609578815140509 1103 108-109 0.07260808221894877 1103 110-111 -0.0011550809272087292 1103 112-113 -0.013994334028812005 1103 114-115 0.013079192885623314 1103 116-117 0.02430861140949858 1103 118-119 -0.00466552382652452 1103 120-121 -0.012633337100389497 1103 122-123 0.01316370490199148 1103 124-125 -0.18663752178928306 1104 1 0.35109119963911084 1104 2 0.475957093470992 1104 3 0.2940430141675989 1104 4 0.2117735061504078 1104 5 0.25465794379275053 1104 6 0.24284029978807098 1104 7 0.28921804014902364 1104 8 0.28179711444934696 1104 9 0.3039309928755216 1104 10-11 0.25501133472698 1104 12-13 0.3051922400682514 1104 14-15 0.29291905848050703 1104 16-17 0.3794368408591211 1104 18-19 0.4273364534301649 1104 20-21 0.33842628044430967 1104 22-23 0.3088398331815725 1104 24-25 0.3039971737177183 1104 26-27 0.32393000502348457 1104 28-29 0.3452130042984365 1104 30-31 0.26943388869839424 1104 32-33 0.29873692651644035 1104 34-35 0.28550871476959117 1104 36-37 0.31397674869872816 1104 38-39 0.20770113420464043 1104 40-41 0.09818419907194453 1104 42-43 0.2025647794920431 1104 44-45 0.18432184026256238 1104 46-47 0.35177810973218016 1104 48-49 0.3290098230066363 1104 50-51 0.40192187437792626 1104 52-53 0.33033266029045905 1104 54-55 0.3297214603233627 1104 56-57 0.35813686493533226 1104 58-59 0.1489812823536667 1104 60-61 0.2091111634539331 1104 62-63 0.3783126650290214 1104 64-65 0.3922361802001859 1104 66-67 0.3447286774861098 1104 68-69 0.43989421795897243 1104 70-71 0.4409396463281965 1104 72-73 0.38381623176007906 1104 74-75 0.4083878065665445 1104 76-77 0.39256215909712466 1104 78-79 0.3752063247512609 1104 80-81 0.42973763772767626 1104 82-83 0.4142290294472559 1104 84-85 0.3409776135429965 1104 86-87 0.3159828991746565 1104 88-89 0.43597189570695605 1104 90-91 0.39952212301574264 1104 92-93 0.3833692952158785 1104 94-95 0.4282081028621967 1104 96-97 0.42285933511335827 1104 98-99 0.4136002873094107 1104 100-101 0.3335753835855506 1104 102-103 0.3881063140255705 1104 104-105 0.4414070470196485 1104 106-107 0.334236214763898 1104 108-109 0.2089190268609009 1104 110-111 0.3169612217645117 1104 112-113 0.13651988638763868 1104 114-115 0.18117937064083023 1104 116-117 0.265943832333825 1104 118-119 0.161375740774794 1104 120-121 0.2594813150072781 1104 122-123 0.2284261477663918 1104 124-125 0.07407108664138917 1105 1 0.1848140897209909 1105 2 0.3586462317300594 1105 3 0.23481372555932012 1105 4 0.11561534863517409 1105 5 0.07307223827847054 1105 6 0.07516452208838587 1105 7 0.0150372454229597 1105 8 0.044465156556825036 1105 9 0.1833173564200834 1105 10-11 0.1595863416950678 1105 12-13 0.20720760925595272 1105 14-15 0.1287971340348193 1105 16-17 0.19440622164818677 1105 18-19 0.18967022908586983 1105 20-21 0.17419339670440337 1105 22-23 0.196185348557826 1105 24-25 0.2088998829927533 1105 26-27 0.06322817675767567 1105 28-29 0.03275781721021076 1105 30-31 0.14495573821183427 1105 32-33 0.08137600054787697 1105 34-35 -0.007856298136530881 1105 36-37 -0.08894081170149803 1105 38-39 0.0074783067346047005 1105 40-41 -0.0054963421771461185 1105 42-43 0.056226890642200544 1105 44-45 0.009171142645449493 1105 46-47 -0.06945265732348105 1105 48-49 -0.13403782864531166 1105 50-51 -0.017846603184821674 1105 52-53 -0.015595247221753539 1105 54-55 -0.0633524075131433 1105 56-57 -0.1946598142133027 1105 58-59 -0.12569232816836262 1105 60-61 -0.03785519176931729 1105 62-63 0.08342338195481602 1105 64-65 0.06174196573645219 1105 66-67 0.08542640063674156 1105 68-69 0.14789580951162407 1105 70-71 0.18272903725634393 1105 72-73 0.20999237503213664 1105 74-75 0.2166503986160464 1105 76-77 0.0639201695755105 1105 78-79 -0.0069608377608147975 1105 80-81 0.08208512739672358 1105 82-83 0.11597461228272721 1105 84-85 0.06924234146424624 1105 86-87 0.054424071308758926 1105 88-89 0.15983574322490313 1105 90-91 0.025132588484304108 1105 92-93 0.0839830680035547 1105 94-95 -0.07844352181414393 1105 96-97 0.04921142271338397 1105 98-99 0.09186554541158642 1105 100-101 0.011134996119228191 1105 102-103 0.055198892704421354 1105 104-105 0.1597004602537737 1105 106-107 0.10866871919721888 1105 108-109 0.06869563891388353 1105 110-111 0.07562740773380483 1105 112-113 0.07812096209949715 1105 114-115 0.04007689222171962 1105 116-117 -0.02058904191331834 1105 118-119 0.00839619969940486 1105 120-121 -0.034742717235801024 1105 122-123 -0.07217015429737828 1105 124-125 -0.18640692953266225 1106 1 0.1481720343511661 1106 2 0.3431059111197179 1106 3 0.14789075691498255 1106 4 0.10126266164824926 1106 5 0.053830986424948435 1106 6 0.01853317090243678 1106 7 0.06414938879137821 1106 8 0.09444034751618346 1106 9 0.12565708702538103 1106 10-11 0.1634027318976976 1106 12-13 0.19513515540896975 1106 14-15 0.20360413218774198 1106 16-17 0.2186516924696562 1106 18-19 0.2284662607842094 1106 20-21 0.21304608460198438 1106 22-23 0.22158118089918588 1106 24-25 0.2550079468546329 1106 26-27 0.27815811951344926 1106 28-29 0.13140705796509877 1106 30-31 0.07633272192685325 1106 32-33 0.2021700095353367 1106 34-35 0.2249963876795391 1106 36-37 0.22711556817682066 1106 38-39 0.1301189435528869 1106 40-41 0.1292799094174839 1106 42-43 0.14458987643821786 1106 44-45 0.11549778625415286 1106 46-47 0.14473672190931097 1106 48-49 0.2261093031459822 1106 50-51 0.2572939184997338 1106 52-53 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-0.5549713016258337 2216 52-53 -0.5243292352723472 2216 54-55 -0.5336508386823908 2216 56-57 -0.7056805340547498 2216 58-59 -0.6722850615984228 2216 60-61 -0.6967484184094133 2216 62-63 -0.718981632395483 2216 64-65 -0.8793183972252052 2216 66-67 -0.8972729951351184 2216 68-69 -0.8103267470521018 2216 70-71 -0.5517622149990089 2216 72-73 -0.6734738421559783 2216 74-75 -0.6625890452507761 2216 76-77 -0.720298750420973 2216 78-79 -0.7577845438929671 2216 80-81 -0.6715943990289688 2216 82-83 -0.6989481770328894 2216 84-85 -0.6033944449185569 2216 86-87 -0.5974279521653045 2216 88-89 -0.6088919264469759 2216 90-91 -0.5876917412800395 2216 92-93 -0.5342879069382072 2216 94-95 -0.5588815268862213 2216 96-97 -0.5091372842152069 2216 98-99 -0.5481098327016269 2216 100-101 -0.5445619466350031 2216 102-103 -0.5947092277396138 2216 104-105 -0.4560805714809959 2216 106-107 -0.5125683701202348 2216 108-109 -0.5026207076827376 2216 110-111 -0.623827614001609 2216 112-113 -0.5689099791313765 2216 114-115 -0.5567596225045897 2216 116-117 -0.4916288810046332 2216 118-119 -0.6347208703491631 2216 120-121 -0.7065798111870478 2216 122-123 -0.78081638976969 2216 124-125 -0.7396477172013469 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82.0 3 9.0 4 36.0 5 104.0 6 308.0 7 637.0 8 1106.0 9 1963.0 10 2991.0 11 4170.0 12 5283.0 13 7398.0 14 10495.0 15 8220.0 16 4516.0 17 4312.0 18 4512.0 19 4477.0 20 4501.0 21 4803.0 22 5251.0 23 6193.0 24 7991.0 25 11059.0 26 14929.0 27 19587.0 28 25798.0 29 32726.0 30 40996.0 31 51074.0 32 64481.0 33 82758.0 34 110676.0 35 161998.0 36 301026.0 37 567173.0 38 236400.0 39 646.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58018511397239 18.782681146928688 12.62956431030901 25.007569428789907 2 15.750364659534197 20.47097276969479 38.50523005446282 25.27343251630819 3 18.964316504010394 24.68711840761619 29.64473352114605 26.70383156722736 4 12.90958553403918 16.359599572865932 36.1950799992041 34.53573489389079 5 13.262408723042196 38.596987342597124 34.12870375535538 14.011900179005291 6 32.90003506960073 36.381535164868545 16.8630104076634 13.855419357867326 7 29.08042123541696 32.761287963684545 20.35100373968769 17.807287061210804 8 27.17595826993652 35.06203453389187 19.3731101765354 18.388897019636214 9 27.25697733178328 15.05352946536808 19.07913303528775 38.6103601675609 10-11 25.401132720489763 26.2826775502089 27.342193700174246 20.973996029127097 12-13 25.971276064031017 23.551004177976843 27.83634370417825 22.64137605381389 14-15 22.794135920936 24.91325106244322 25.459812170532146 26.832800846088634 16-17 22.70389383023552 28.62507835432447 26.08545937034879 22.585568445091223 18-19 22.65089398561372 26.879352465519236 28.14961863848652 22.320134910380528 20-21 23.32965148548754 26.140024355423503 28.268472981219816 22.26185117786915 22-23 23.081817102367335 26.179871153734634 28.01867801412173 22.7196337297763 24-25 22.817939067259076 26.43946904072216 28.10566166947868 22.636930222540087 26-27 22.594686452306284 26.791080777401337 27.938084917021204 22.67614785327117 28-29 22.551188938677104 26.71172182444118 28.12448655041685 22.612602686464868 30-31 22.62985001099043 26.337408445290293 28.24676113650999 22.78598040720928 32-33 22.617931887655775 26.431764774104828 28.038035329171002 22.91226800906839 34-35 23.091509566821397 26.460124207137074 27.766701552175004 22.68166467386652 36-37 23.201882160618773 26.136821147797658 27.726192021251627 22.935104670331945 38-39 22.793556029900287 26.26539127457288 27.880663947622033 23.0603887479048 40-41 22.946288675792985 26.076333449497174 28.030730213993678 22.946647660716167 42-43 23.09892727465022 25.95930729451581 27.787002424795045 23.15476300603893 44-45 22.84632611415017 26.324512546356765 27.71652717065641 23.112634168836657 46-47 23.11268939655434 26.558926594079036 27.22292944382927 23.10545456553735 48-49 23.171727826761696 26.50883505413697 27.08044193219693 23.238995186904404 50-51 23.192202504688836 26.529007822458254 27.029785429152742 23.24900424370017 52-53 23.386674103999315 26.45139822774254 27.002791761129075 23.15913590712907 54-55 22.87819250725554 26.36830812648252 27.124071829169623 23.629427537092315 56-57 22.96266698183441 26.66945936213878 27.107279270283584 23.260594385743225 58-59 23.105157294670835 26.248178858968853 27.09736352902062 23.549300317339693 60-61 22.863354424891906 26.408685811991866 27.52986866238429 23.198091100731936 62-63 22.80924361951006 26.40718162719338 27.39076455423278 23.392810199063774 64-65 22.598326047876906 26.2718805314011 27.364698995131675 23.765094425590316 66-67 22.658122565837 26.544526819699076 27.10351447298369 23.693836141480237 68-69 22.67315960534273 26.283505965974204 27.409184921728517 23.63414950695455 70-71 22.641845489414226 26.307806161756464 27.278433300104656 23.771915048724654 72-73 22.718473947704872 26.37783490778352 27.006906226096756 23.896784918414856 74-75 22.569138199079354 26.597585996459905 26.952341241022044 23.8809345634387 76-77 22.816806899046494 26.45101163371873 26.945410162452333 23.786771304782445 78-79 22.906496712570103 26.358836572899207 27.016598690550815 23.718068023979875 80-81 22.80018335602272 26.76741122834728 26.768129188677214 23.664276226952783 82-83 22.80548521692067 26.634533339592476 26.793423483377836 23.76655796010902 84-85 22.83171838282197 26.445074654067387 26.695311442906966 24.02789552020368 86-87 22.750340340810247 26.319652507200313 26.85251714130288 24.077490010686564 88-89 22.773204615932645 26.377917749360048 26.74142658717557 24.107451047531736 90-91 22.732540720255265 26.556799871871164 26.7869347247094 23.923724683164174 92-93 22.686618682785863 26.05447408497648 26.93643677573726 24.322470456500398 94-95 22.613634951558186 26.098325074454177 26.905453897781356 24.38258607620628 96-97 22.717155678287885 26.01038315498792 26.893144632378323 24.379316534345875 98-99 22.719771069903548 26.01903663555387 26.598404095201957 24.66278819934063 100-101 22.433723140081582 26.021416401270148 26.86374366383359 24.68111679481468 102-103 22.44857887159762 25.61773152452989 27.04111066710006 24.892578936772427 104-105 22.41409163172672 25.83498954632053 26.8580445281733 24.89287429377946 106-107 22.603918172292868 25.892674181035268 26.582240027130823 24.92116761954104 108-109 22.600852276650215 25.878250927258218 26.45310959460243 25.06778720148914 110-111 22.40914885249958 26.113063256126335 26.662925617935823 24.81486227343826 112-113 22.294773131205794 26.0784234670602 26.707770768460946 24.91903263327306 114-115 22.109603317520232 26.217622103251937 26.453838292151982 25.21893628707585 116-117 22.10286278555507 26.262754848444015 26.580314400524884 25.05406796547603 118-119 22.07197927827422 26.40294146885666 26.234273690256593 25.29080556261253 120-121 22.06539607201626 26.524397399974433 26.352472517837967 25.057734010171345 122-123 21.989052127898407 26.498981179081905 26.293063098414045 25.218903594605646 124-125 21.87345222629422 26.579568240712533 26.209801390506776 25.337178142486476 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 62.0 1 48.5 2 80.0 3 143.5 4 117.0 5 85.0 6 208.5 7 504.5 8 879.0 9 1032.0 10 810.0 11 544.5 12 486.5 13 555.5 14 643.0 15 734.0 16 832.5 17 948.5 18 1125.0 19 1251.5 20 1340.5 21 1583.5 22 1954.5 23 2416.0 24 3074.5 25 4135.5 26 5510.5 27 7322.5 28 9739.5 29 12561.0 30 16508.0 31 20737.0 32 25341.5 33 28750.5 34 31353.0 35 34549.5 36 38977.5 37 43992.5 38 47164.0 39 49683.0 40 51812.5 41 55912.0 42 59992.5 43 63087.5 44 66317.0 45 69576.0 46 70339.5 47 70221.5 48 71714.0 49 70435.0 50 68891.5 51 67791.0 52 67861.0 53 69190.0 54 68352.5 55 63586.0 56 56530.5 57 50804.5 58 47535.5 59 42640.5 60 35496.5 61 29088.0 62 23630.5 63 18262.5 64 13663.5 65 10456.5 66 7501.0 67 6273.5 68 4731.5 69 2790.0 70 2019.5 71 1657.5 72 1391.5 73 1054.0 74 867.5 75 751.5 76 432.5 77 145.5 78 62.5 79 30.5 80 16.0 81 5.5 82 4.5 83 2.0 84 1.5 85 1.5 86 0.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04269102577201446 2 0.005080950027199651 3 8.836434829912436E-4 4 0.07825767596241201 5 0.006848236993182138 6 0.0 7 0.0058541380748169895 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 8.28415765304291E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.351187533999564E-4 28-29 8.560296241477672E-4 30-31 0.0010493266360521018 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0013254652244868656 42-43 9.1125734183472E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 5.5227717686952726E-5 52-53 0.0 54-55 0.0 56-57 4.970494591825746E-4 58-59 3.8659402380866904E-4 60-61 4.418217414956218E-4 62-63 4.970494591825746E-4 64-65 0.0 66-67 5.5227717686952726E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 4.142078826521454E-4 92-93 4.142078826521454E-4 94-95 4.142078826521454E-4 96-97 0.0033136630612171634 98-99 0.00972007831290368 100-101 0.018970721025468262 102-103 0.009361098147938488 104-105 0.004335375838425789 106-107 0.010935088102016639 108-109 0.013254652244868654 110-111 0.007787108193860335 112-113 0.004362989697269265 114-115 0.0030651383316258763 116-117 5.5227717686952726E-5 118-119 0.0013806929421738181 120-121 5.798910357130036E-4 122-123 7.455741887738618E-4 124-125 0.03454493741318893 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1810685.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.74833144212799 #Duplication Level Percentage of deduplicated Percentage of total 1 79.77350390612486 50.8542776730754 2 11.590436900075881 14.777420261302158 3 3.8261886951333874 7.317394352924592 4 1.7418795424112847 4.441676576075873 5 0.9161518339949428 2.9201575382411518 6 0.5351107258955006 2.0467449547574446 7 0.3405232229669933 1.5195451097009078 8 0.2428073918633685 1.2382852874483734 9 0.16917682613650661 0.9706266346389559 >10 0.7957780741334199 8.862062327879293 >50 0.04698683309192852 2.051756616332096 >100 0.02053121853896841 2.1260837948296754 >500 3.699318532445592E-4 0.15198945993408636 >1k 5.548977798668388E-4 0.7219794128598997 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2508 0.13851111595887744 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2286 0.12625056263237394 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.7613858843476363E-5 0.0 12-13 2.7613858843476363E-5 0.0 0.0 5.5227717686952726E-5 0.0 14-15 5.5227717686952726E-5 0.0 0.0 5.5227717686952726E-5 0.0 16-17 5.5227717686952726E-5 0.0 0.0 5.5227717686952726E-5 0.0 18-19 5.5227717686952726E-5 0.0 0.0 5.5227717686952726E-5 0.0 20-21 5.5227717686952726E-5 0.0 0.0 8.284157653042908E-5 0.0 22-23 5.5227717686952726E-5 0.0 0.0 1.3806929421738182E-4 0.0 24-25 1.1045543537390545E-4 0.0 0.0 3.865940238086691E-4 0.0 26-27 1.1045543537390545E-4 0.0 0.0 7.455741887738618E-4 0.0 28-29 2.7613858843476363E-4 0.0 0.0 0.0032032076258432583 0.0 30-31 2.7613858843476363E-4 0.0 0.0 0.013944998715955562 0.0 32-33 3.0375244727824E-4 0.0 0.0 0.036726432261823566 0.0 34-35 3.589801649651927E-4 0.0 0.0 0.06757111258998666 0.0 36-37 3.865940238086691E-4 0.0 0.0 0.1229092857123133 0.0 38-39 3.865940238086691E-4 0.0 0.0 0.21317899027163753 0.0 40-41 3.865940238086691E-4 0.0 0.0 0.3109872782952308 0.0 42-43 4.142078826521455E-4 0.0 0.0 0.40661407147018946 0.0 44-45 4.418217414956218E-4 0.0 0.0 0.517594170162121 0.0 46-47 4.418217414956218E-4 0.0 0.0 0.65770688993392 0.0 48-49 4.418217414956218E-4 0.0 0.0 0.8084509453604576 0.0 50-51 4.418217414956218E-4 0.0 0.0 0.9728638609145157 0.0 52-53 4.418217414956218E-4 0.0 0.0 1.1334660639481742 0.0 54-55 4.418217414956218E-4 0.0 0.0 1.305251879813441 0.0 56-57 4.418217414956218E-4 0.0 0.0 1.5051762178402095 0.0 58-59 4.418217414956218E-4 0.0 0.0 1.7270259597886988 0.0 60-61 4.418217414956218E-4 0.0 0.0 1.972181798601082 0.0 62-63 4.418217414956218E-4 0.0 0.0 2.214797162399865 0.0 64-65 4.418217414956218E-4 0.0 0.0 2.4551205759146404 0.0 66-67 4.418217414956218E-4 0.0 0.0 2.7376379657422465 0.0 68-69 4.970494591825745E-4 0.0 0.0 3.055970530489842 0.0 70-71 4.970494591825745E-4 0.0 0.0 3.4016408154924793 0.0 72-73 4.970494591825745E-4 0.0 0.0 3.753358535581838 0.0 74-75 4.970494591825745E-4 0.0 0.0 4.097896652371892 0.0 76-77 4.970494591825745E-4 0.0 0.0 4.479879161753701 0.0 78-79 4.970494591825745E-4 0.0 0.0 4.912505488254444 0.0 80-81 4.970494591825745E-4 0.0 0.0 5.3774676434609 0.0 82-83 4.970494591825745E-4 0.0 0.0 5.854358985687737 0.0 84-85 5.246633180260509E-4 0.0 0.0 6.32136456644861 0.0 86-87 5.522771768695273E-4 0.0 0.0 6.818414025631183 0.0 88-89 5.522771768695273E-4 0.0 0.0 7.355227441548364 0.0 90-91 6.627326122434328E-4 0.0 0.0 7.933820625895724 0.0 92-93 6.627326122434328E-4 0.0 0.0 8.528512689948831 0.0 94-95 6.627326122434328E-4 0.0 0.0 9.122072585789356 0.0 96-97 6.627326122434328E-4 0.0 0.0 9.719498421867968 0.0 98-99 7.731880476173382E-4 0.0 0.0 10.359449600565533 0.0 100-101 9.388712006781964E-4 0.0 0.0 11.060537862742553 0.0 102-103 0.0010493266360521018 0.0 0.0 11.794044795201817 0.0 104-105 0.00121500978911296 0.0 0.0 12.526861381189992 0.0 106-107 0.0012426236479564363 0.0 0.0 13.25230506686696 0.0 108-109 0.0012702375067999128 0.0 0.0 14.007461264659508 0.0 110-111 0.0012702375067999128 0.0 0.0 14.816160734749555 0.0 112-113 0.0012702375067999128 0.0 0.0 15.649685063939891 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2815 0.0 67.9533 1 GTATCAA 4840 0.0 52.819683 1 ATCAACG 6075 0.0 41.860203 3 TCAACGC 6150 0.0 41.252872 4 CAACGCA 6185 0.0 41.080513 5 AACGCAG 6330 0.0 40.137276 6 TATCAAC 6395 0.0 39.306435 2 GTACATG 9380 0.0 37.60995 1 TACATGG 9770 0.0 35.300083 2 ACATGGG 9845 0.0 34.601906 3 ACGCAGA 7820 0.0 32.413548 7 CGCAGAG 7850 0.0 32.058743 8 CATGGGA 6865 0.0 30.796936 4 GTGGTAT 1530 0.0 28.432518 1 GCAGAGT 8940 0.0 27.684174 9 CATGGGG 3845 0.0 27.10575 4 ATGGGAG 3055 0.0 26.4896 5 GAGTACA 7195 0.0 26.00655 1 TGGTATC 1445 0.0 25.96955 2 AGTACAT 7295 0.0 24.740536 2 >>END_MODULE