FastQCFastQC Report
Thu 26 May 2016
SRR1364449_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364449_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184254
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.050.310751
GTATCAA32950.048.2376061
ATCAACG35100.043.886873
CAACGCA36350.042.7098245
TCAACGC37450.041.4505844
AACGCAG37950.040.5938076
GTACATG105050.039.3272251
TACATGG108200.037.433622
TATCAAC42850.036.7821432
ACATGGG105900.036.1124533
ACGCAGA49350.031.3370427
CGCAGAG50400.030.566178
CATGGGA71700.029.8624444
CATGGGG44500.029.1365724
ATGGGAT32600.026.4570885
GAGTACA75500.023.811671
AGTACAT77650.022.2125552
GCAGAGT67000.022.1052999
TAAGGTG9900.021.6301255
GTACTAG4151.8189894E-1221.5165731
Produced by FastQC (version 0.11.5)