##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364449_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2184254 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.691654908266162 31.0 31.0 34.0 27.0 34.0 2 30.74634497636264 31.0 31.0 34.0 27.0 34.0 3 30.677825930500756 31.0 31.0 34.0 27.0 34.0 4 34.24853794476284 37.0 35.0 37.0 30.0 37.0 5 34.42072671035511 37.0 35.0 37.0 31.0 37.0 6 34.55767781585841 37.0 35.0 37.0 31.0 37.0 7 34.45027638726998 37.0 35.0 37.0 30.0 37.0 8 34.315191822929016 37.0 35.0 37.0 30.0 37.0 9 35.43921357131543 39.0 35.0 39.0 29.0 39.0 10-11 35.66294144362331 39.0 35.0 39.0 29.5 39.0 12-13 35.71644895694365 39.0 35.0 39.0 30.0 39.0 14-15 36.86720202870179 40.0 37.0 41.0 30.0 41.0 16-17 37.00834014725393 40.0 37.0 41.0 30.5 41.0 18-19 36.996125908433726 40.0 37.0 41.0 30.0 41.0 20-21 36.94449500836441 40.0 37.0 41.0 30.0 41.0 22-23 36.85898160195655 40.0 37.0 41.0 30.0 41.0 24-25 36.91904947867785 40.0 37.0 41.0 30.0 41.0 26-27 36.928520446797855 40.0 37.0 41.0 30.0 41.0 28-29 36.8734854096639 40.0 36.0 41.0 30.0 41.0 30-31 36.65059832785015 40.0 36.0 41.0 30.0 41.0 32-33 36.724157080632565 40.0 36.0 41.0 30.0 41.0 34-35 36.67688304565311 40.0 36.0 41.0 30.0 41.0 36-37 36.567700230833964 40.0 36.0 41.0 30.0 41.0 38-39 36.43454401365409 40.0 36.0 41.0 29.5 41.0 40-41 36.41276861573792 40.0 36.0 41.0 29.0 41.0 42-43 36.292697186316246 40.0 35.0 41.0 29.0 41.0 44-45 36.27931046480858 40.0 35.0 41.0 29.0 41.0 46-47 36.15107011364063 39.5 35.0 41.0 29.0 41.0 48-49 35.990043053600914 39.0 35.0 41.0 28.0 41.0 50-51 35.87226966277731 39.0 35.0 41.0 28.0 41.0 52-53 35.756117649321006 39.0 35.0 41.0 28.0 41.0 54-55 35.645330854378656 39.0 35.0 40.5 28.0 41.0 56-57 35.61369602619476 39.0 34.5 40.5 28.0 41.0 58-59 35.598579881277544 39.0 35.0 41.0 28.0 41.0 60-61 35.422353352677845 38.5 34.0 41.0 28.0 41.0 62-63 35.17516094739898 38.0 34.0 40.0 28.0 41.0 64-65 34.8862176743181 37.5 34.0 40.0 28.0 41.0 66-67 34.58330601660796 37.0 34.0 40.0 27.0 41.0 68-69 34.28539858459685 36.5 34.0 39.5 27.0 41.0 70-71 33.93280566271139 36.0 33.5 39.0 26.0 41.0 72-73 33.596804217824484 35.5 33.0 39.0 26.0 40.5 74-75 33.23241916919919 35.0 33.0 37.5 26.0 40.0 76-77 32.87048369832446 35.0 33.0 37.0 26.0 39.0 78-79 32.537499072910016 35.0 33.0 37.0 26.0 39.0 80-81 32.200254869625965 35.0 32.0 36.0 25.0 38.5 82-83 31.907074223052813 35.0 32.0 36.0 25.0 37.0 84-85 31.549532929778316 35.0 32.0 35.5 24.0 37.0 86-87 31.34418364347736 35.0 32.0 35.0 23.5 36.5 88-89 31.199566076106535 35.0 32.0 35.0 23.0 36.0 90-91 31.065363277347778 35.0 32.0 35.0 23.0 36.0 92-93 30.83598496328724 35.0 32.0 35.0 20.5 36.0 94-95 30.754354347067697 35.0 31.5 35.0 20.5 35.0 96-97 30.67437372210375 35.0 31.5 35.0 20.0 35.0 98-99 30.516435588535032 34.5 31.0 35.0 19.5 35.0 100-101 30.38409727073866 34.0 31.0 35.0 18.5 35.0 102-103 29.348819322294936 33.5 29.5 34.5 17.5 35.0 104-105 30.288592352354627 34.0 31.0 35.0 19.0 35.0 106-107 30.528348809250204 35.0 32.0 35.0 19.0 35.0 108-109 30.517247994051974 35.0 32.0 35.0 18.5 35.0 110-111 30.39967810520205 35.0 32.0 35.0 17.5 35.0 112-113 30.279302224008745 35.0 32.0 35.0 13.0 35.0 114-115 30.17251221698575 35.0 32.0 35.0 7.5 35.0 116-117 30.047865999100836 35.0 31.5 35.0 2.0 35.0 118-119 29.78754165037583 35.0 31.0 35.0 2.0 35.0 120-121 29.64893093935046 35.0 31.0 35.0 2.0 35.0 122-123 29.412622341540867 35.0 31.0 35.0 2.0 35.0 124-125 28.244968304968197 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6097925751039242 1101 2 0.6209839659462872 1101 3 0.742007287868713 1101 4 0.7024544663041468 1101 5 0.653315177149679 1101 6 0.5782582837323176 1101 7 0.6797629023352627 1101 8 0.8244352073246262 1101 9 1.305758331694463 1101 10-11 0.9811453739418639 1101 12-13 0.8294894359275631 1101 14-15 1.0226912597275657 1101 16-17 0.8035427303212614 1101 18-19 0.895043856075489 1101 20-21 0.9353607063881313 1101 22-23 0.891043293921804 1101 24-25 0.9715731525139191 1101 26-27 0.884883337497989 1101 28-29 0.8343618153674868 1101 30-31 1.0429618406528292 1101 32-33 0.8890828736127361 1101 34-35 0.7743196107117072 1101 36-37 0.8604355439811684 1101 38-39 0.9381218369832993 1101 40-41 0.8690570815930272 1101 42-43 0.8803380455216896 1101 44-45 0.8124899902956884 1101 46-47 0.804171664777094 1101 48-49 0.7745292496805405 1101 50-51 0.7645373888219353 1101 52-53 0.6172232570015126 1101 54-55 0.6571716249109016 1101 56-57 0.6530983729749238 1101 58-59 0.8382190359413428 1101 60-61 0.7736329048270392 1101 62-63 0.7523356488773842 1101 64-65 0.6475052635832057 1101 66-67 0.6548207016770178 1101 68-69 0.5632540024933803 1101 70-71 0.5512841071039247 1101 72-73 0.624888758649611 1101 74-75 0.6397767808615598 1101 76-77 0.6689050804437713 1101 78-79 0.5773996727469282 1101 80-81 0.606926980526616 1101 82-83 0.5789702861851218 1101 84-85 0.7522409201786857 1101 86-87 0.7314930140830711 1101 88-89 0.6253491155052195 1101 90-91 0.5909954878407611 1101 92-93 0.7258716093551953 1101 94-95 0.7056489697890065 1101 96-97 0.5894624866814766 1101 98-99 0.6247972901828653 1101 100-101 0.5759760503805467 1101 102-103 0.4869282908063397 1101 104-105 0.5308742261792752 1101 106-107 0.4857800519640172 1101 108-109 0.434552631776139 1101 110-111 0.4601819670241092 1101 112-113 0.46424289606856206 1101 114-115 0.39995203527138656 1101 116-117 0.41679723721121675 1101 118-119 0.37725231496681744 1101 120-121 0.15417757769159124 1101 122-123 0.29690328220447526 1101 124-125 -0.11021652153116435 1102 1 0.2609499846738501 1102 2 0.23844322588662692 1102 3 0.3262217347513854 1102 4 0.19333576341710312 1102 5 0.2458411223097272 1102 6 0.11611242834471369 1102 7 0.23611170401723314 1102 8 0.2938216639682949 1102 9 0.6779301531388526 1102 10-11 0.2618564436637527 1102 12-13 0.34296538180055336 1102 14-15 0.4847665086816235 1102 16-17 0.2889873812201387 1102 18-19 0.30579240719312395 1102 20-21 0.5465130810703158 1102 22-23 0.49786045325190287 1102 24-25 0.33773821521154446 1102 26-27 0.3144472739344977 1102 28-29 0.3293880936463154 1102 30-31 0.3836168382460059 1102 32-33 0.3200069672714818 1102 34-35 0.10542262972676752 1102 36-37 0.1462507311496708 1102 38-39 0.27072838951600886 1102 40-41 0.25748367425733676 1102 42-43 0.2786445236619741 1102 44-45 0.22386780404319495 1102 46-47 0.29260086338915414 1102 48-49 0.2487123711044319 1102 50-51 0.18254089849270372 1102 52-53 0.16689616138929608 1102 54-55 0.07641448399942874 1102 56-57 0.2164326880458063 1102 58-59 0.34510198316008456 1102 60-61 0.2181986924698336 1102 62-63 0.23505226582617667 1102 64-65 0.22990523329520585 1102 66-67 0.11438352198118906 1102 68-69 0.1305340174800449 1102 70-71 0.2619387457646809 1102 72-73 0.15539226217428137 1102 74-75 0.24864236263448447 1102 76-77 0.23514832157408705 1102 78-79 0.10854251851071695 1102 80-81 0.1802803288498822 1102 82-83 0.2048445447806877 1102 84-85 0.33351861144664596 1102 86-87 0.2695639440474835 1102 88-89 0.17538921268248586 1102 90-91 0.22228003815989084 1102 92-93 0.18426834179172147 1102 94-95 0.24916593721318847 1102 96-97 0.16909626743473183 1102 98-99 0.06853679571165472 1102 100-101 0.10735938087028174 1102 102-103 0.08114967541030182 1102 104-105 0.09850909258019769 1102 106-107 0.051065828553657155 1102 108-109 0.06421898687634453 1102 110-111 0.07993357595100647 1102 112-113 0.026012560771590643 1102 114-115 0.027670518177107084 1102 116-117 0.010432086894741843 1102 118-119 0.060661622330488996 1102 120-121 -0.12989659053167557 1102 122-123 -0.24086712789794973 1102 124-125 -0.4842031151591826 1103 1 0.3836371665789535 1103 2 0.42400270832913733 1103 3 0.5841750738045306 1103 4 0.41955019795931037 1103 5 0.5282647663256839 1103 6 0.4020102289261658 1103 7 0.49219087758989133 1103 8 0.6650093165522577 1103 9 1.0647418075296358 1103 10-11 0.7217340854142691 1103 12-13 0.5842667091639129 1103 14-15 0.8277695329577739 1103 16-17 0.7185377707562424 1103 18-19 0.7665990140467329 1103 20-21 0.6669614762903038 1103 22-23 0.7108574531845591 1103 24-25 0.544816689130343 1103 26-27 0.6886253871900436 1103 28-29 0.6465981184382557 1103 30-31 0.8509850416822573 1103 32-33 0.7635950570358361 1103 34-35 0.6776270427817366 1103 36-37 0.5965658924077388 1103 38-39 0.6784005461070564 1103 40-41 0.6888623093856836 1103 42-43 0.7396156927928885 1103 44-45 0.6493861295850394 1103 46-47 0.6414170765296774 1103 48-49 0.5964916401577938 1103 50-51 0.5803577632537298 1103 52-53 0.642181943045216 1103 54-55 0.5383104393314895 1103 56-57 0.541238467572903 1103 58-59 0.5326269905260048 1103 60-61 0.6554184238423275 1103 62-63 0.7074154579423961 1103 64-65 0.6738477131593825 1103 66-67 0.5308965539590105 1103 68-69 0.5442084222242798 1103 70-71 0.6417823933485636 1103 72-73 0.5607939300653157 1103 74-75 0.5473453275318008 1103 76-77 0.5118445642993166 1103 78-79 0.33249885695874326 1103 80-81 0.4081724244135856 1103 82-83 0.4227361436794901 1103 84-85 0.5113731593360455 1103 86-87 0.58748841697507 1103 88-89 0.4978157559918053 1103 90-91 0.5445796773429272 1103 92-93 0.4319869255032458 1103 94-95 0.5046523311406936 1103 96-97 0.4916256981923901 1103 98-99 0.5094848281357081 1103 100-101 0.5002673807584621 1103 102-103 0.35717061718494136 1103 104-105 0.33846947274691885 1103 106-107 0.46096927862629755 1103 108-109 0.42384606742914954 1103 110-111 0.3976989143704941 1103 112-113 0.5039626085791866 1103 114-115 0.5039419923294481 1103 116-117 0.4098745445194787 1103 118-119 0.3494293678935776 1103 120-121 0.21462227038995252 1103 122-123 0.31441931277561963 1103 124-125 0.03592732806476562 1104 1 0.4250305877384477 1104 2 0.4607730737558775 1104 3 0.5605742156439497 1104 4 0.4161973828556711 1104 5 0.447892841455932 1104 6 0.47481776809094356 1104 7 0.48273122652387457 1104 8 0.5915642233163965 1104 9 0.9769127625203708 1104 10-11 0.8057768362405966 1104 12-13 0.6466436185473157 1104 14-15 0.7565589437011511 1104 16-17 0.590138905824233 1104 18-19 0.6618510344928126 1104 20-21 0.7576019157149574 1104 22-23 0.7475879320459882 1104 24-25 0.7636110403994749 1104 26-27 0.6966210435656137 1104 28-29 0.7043397582366993 1104 30-31 0.7525509026042485 1104 32-33 0.7032214898311224 1104 34-35 0.6742494394171601 1104 36-37 0.5155526276234141 1104 38-39 0.7423035109723486 1104 40-41 0.7135305500618543 1104 42-43 0.5980686384991216 1104 44-45 0.5262057794828578 1104 46-47 0.4948555373283057 1104 48-49 0.5710217921927665 1104 50-51 0.6221655990663919 1104 52-53 0.5410303795955187 1104 54-55 0.4592494979779005 1104 56-57 0.4961777809524932 1104 58-59 0.37253755087500195 1104 60-61 0.5556012350131923 1104 62-63 0.5440894375967389 1104 64-65 0.4501177487219792 1104 66-67 0.4669560912153585 1104 68-69 0.38291580545737247 1104 70-71 0.417821672736693 1104 72-73 0.4206621953314382 1104 74-75 0.42781430520352615 1104 76-77 0.503499528653343 1104 78-79 0.39332537329496375 1104 80-81 0.4490954995893546 1104 82-83 0.42948323788687404 1104 84-85 0.4171047551114384 1104 86-87 0.4777834162093342 1104 88-89 0.3739903252011274 1104 90-91 0.4150064347472515 1104 92-93 0.35632945744472266 1104 94-95 0.3016345812135022 1104 96-97 0.23789175230957937 1104 98-99 0.31253263044905566 1104 100-101 0.3740192354837468 1104 102-103 0.31589099667789355 1104 104-105 0.30696819020230137 1104 106-107 0.24134316852910231 1104 108-109 0.09669916291055713 1104 110-111 0.2261792921183492 1104 112-113 0.1650342492673076 1104 114-115 0.09947126271357476 1104 116-117 0.138541203794734 1104 118-119 0.04371006709560277 1104 120-121 0.03821192425659348 1104 122-123 0.10583339833081595 1104 124-125 -0.0693506695773749 1105 1 0.24281935802768118 1105 2 0.2828453894406273 1105 3 0.2642877758789979 1105 4 0.20721198642736738 1105 5 0.19458616359446523 1105 6 0.2547754556082822 1105 7 0.32831708109645064 1105 8 0.4131318627810572 1105 9 0.8408610672544938 1105 10-11 0.47883459583159294 1105 12-13 0.4736996007498746 1105 14-15 0.25068176377654083 1105 16-17 0.28469497994836246 1105 18-19 0.2177028065801352 1105 20-21 0.5416554233530206 1105 22-23 0.6495852986267323 1105 24-25 0.6166102361303132 1105 26-27 0.5945330316026016 1105 28-29 0.4955483788026456 1105 30-31 0.6960639792068761 1105 32-33 0.4985148196089568 1105 34-35 0.5447948574211594 1105 36-37 0.5199332261381713 1105 38-39 0.676211006096942 1105 40-41 0.5194078460039862 1105 42-43 0.5689116503710991 1105 44-45 0.5836936217390019 1105 46-47 0.6297596408145054 1105 48-49 0.46957812369730334 1105 50-51 0.5157042173726651 1105 52-53 0.44777045322559417 1105 54-55 0.4200117919977089 1105 56-57 0.3144477562246948 1105 58-59 0.41826616947043505 1105 60-61 0.3828553840675113 1105 62-63 0.4035218941588923 1105 64-65 0.4135668300595441 1105 66-67 0.3545505517892167 1105 68-69 0.3929573897069787 1105 70-71 0.4505987199015635 1105 72-73 0.35478958828206686 1105 74-75 0.4056504963161558 1105 76-77 0.3373942529260958 1105 78-79 0.30446608077338766 1105 80-81 0.30632000884416044 1105 82-83 0.22942449732311232 1105 84-85 0.3930793112194806 1105 86-87 0.35021813493467135 1105 88-89 0.27311956015303096 1105 90-91 0.3656814514626987 1105 92-93 0.34506843979613677 1105 94-95 0.3353859970734909 1105 96-97 0.2640285812329992 1105 98-99 0.32021905900493053 1105 100-101 0.2451973505809839 1105 102-103 0.18370277413415437 1105 104-105 0.18638502188158057 1105 106-107 0.19389133275707948 1105 108-109 0.23940035781963687 1105 110-111 0.20115725771362136 1105 112-113 0.33846393426010124 1105 114-115 0.29152082290007897 1105 116-117 0.02893503330466629 1105 118-119 -0.11392476936074303 1105 120-121 -0.10275106044230142 1105 122-123 -0.11680708193174638 1105 124-125 -0.19730612670247538 1106 1 0.5594939820096343 1106 2 0.5102759304543056 1106 3 0.6557321961257578 1106 4 0.5638277960772129 1106 5 0.5311809491797206 1106 6 0.48635322370698475 1106 7 0.45538877511115317 1106 8 0.6580067235280396 1106 9 1.1681377668393154 1106 10-11 0.5987293498667796 1106 12-13 0.7961424058012341 1106 14-15 0.9577162747925314 1106 16-17 0.7781712029318797 1106 18-19 0.7621487750133298 1106 20-21 0.9319001387184613 1106 22-23 0.889634989966666 1106 24-25 0.8699481204895818 1106 26-27 0.9101566268572441 1106 28-29 0.7763656997839945 1106 30-31 0.8608296153314114 1106 32-33 0.863223358506275 1106 34-35 0.8675538610536009 1106 36-37 0.8331884730801917 1106 38-39 0.9212277373993416 1106 40-41 0.9197791175829693 1106 42-43 0.9051795927210549 1106 44-45 0.6135435007040542 1106 46-47 0.7749319585710452 1106 48-49 0.7464722788398319 1106 50-51 0.7325661872688869 1106 52-53 0.6853458732182816 1106 54-55 0.5732953362697017 1106 56-57 0.5662611864976768 1106 58-59 0.76716565182614 1106 60-61 0.7062741817590492 1106 62-63 0.7879247883880183 1106 64-65 0.7989640396943187 1106 66-67 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-0.9829222806196718 2216 78-79 -0.92035558560206 2216 80-81 -0.9585341751598122 2216 82-83 -1.0835105394838003 2216 84-85 -1.2162965062869304 2216 86-87 -1.0682215026132198 2216 88-89 -1.5560435650141748 2216 90-91 -1.3534673991171182 2216 92-93 -1.193112626335875 2216 94-95 -1.163499356074471 2216 96-97 -1.0552583261272979 2216 98-99 -1.162640083591878 2216 100-101 -1.2358933868307744 2216 102-103 -1.4356692032515852 2216 104-105 -1.288758587772623 2216 106-107 -1.0100473329145139 2216 108-109 -0.9561076881709916 2216 110-111 -1.1223534121949363 2216 112-113 -1.3210888841811652 2216 114-115 -1.1735440741521117 2216 116-117 -1.1437495911088256 2216 118-119 -0.92430659482973 2216 120-121 -0.8447351787298665 2216 122-123 -0.7837703954524287 2216 124-125 -0.7508874094212388 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31877.0 3 6477.0 4 4176.0 5 3483.0 6 4040.0 7 4718.0 8 5127.0 9 5746.0 10 6222.0 11 6960.0 12 7449.0 13 8030.0 14 8403.0 15 8104.0 16 7638.0 17 7129.0 18 6786.0 19 6641.0 20 6735.0 21 7161.0 22 8446.0 23 11220.0 24 15761.0 25 21959.0 26 26521.0 27 29365.0 28 34736.0 29 42477.0 30 50840.0 31 61458.0 32 77463.0 33 100987.0 34 137487.0 35 200416.0 36 354155.0 37 607817.0 38 249665.0 39 579.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.61646324558771 18.742447605802255 12.355293034040477 24.285796114569564 2 15.541015068815508 20.295058340498276 39.80288850248553 24.361038088200687 3 18.494689720151595 25.51557648515237 29.434168370528337 26.555565424167703 4 12.162189384546922 16.35518896517371 37.759933559562896 33.72268809071647 5 12.962050699788941 39.19184334983037 34.81020771622036 13.035898234160323 6 32.07085285762357 37.010637542949624 17.763208797547893 13.15530080187891 7 28.57647770334087 32.58164927016172 21.53832137423767 17.303551652259745 8 26.2834065705655 35.704959102217444 20.247126442044 17.764507885173053 9 26.698818215405918 15.87903155286578 19.83629080883548 37.585859422892824 10-11 24.475075095562122 26.9689505805841 28.379815393462742 20.176158930391033 12-13 24.983443674499323 24.462683427261243 28.15264371268246 22.40122918555698 14-15 22.404954394408474 25.868891593206 25.172652028558325 26.553501983827204 16-17 22.538407073892714 29.08515037882282 26.083869131977583 22.292573415306883 18-19 22.631425194863827 28.17092429764085 27.26545941067629 21.932191096819036 20-21 23.261801238752017 27.472701826075152 27.110115131635432 22.155381803537402 22-23 23.046161369282714 27.393735628657794 26.875817867304264 22.68428513475523 24-25 22.783032841566808 27.62325717904187 27.039657404130253 22.554052575261068 26-27 22.602187147441064 27.894028109478246 26.808032116745544 22.695752626335146 28-29 22.6977097055702 27.694742789432286 27.03682455727476 22.570722947722754 30-31 22.782187854381156 27.50784231974685 26.84959345405274 22.860376371819253 32-33 22.553187008444954 27.549928383504557 26.905666070034222 22.991218538016263 34-35 23.125206452370538 27.480925117221457 26.722874361814664 22.67099406859335 36-37 23.132566415519168 27.272539980707254 26.695840797787422 22.899052805986152 38-39 22.663068173364053 27.409668547177002 26.938957238133497 22.988306041325444 40-41 22.88736223047282 27.151244255096273 27.104957677082215 22.85643583734869 42-43 22.740488541656887 27.01948017907746 27.058052751254525 23.181978528011125 44-45 22.632701464654335 27.366325654903058 27.00784782593269 22.993125054509918 46-47 22.9925099120052 27.551666040966573 26.626919449506005 22.82890459752223 48-49 22.759421948776648 27.268221490914296 26.863013306624694 23.10934325368436 50-51 22.828716578470488 27.33548947735179 26.94643581518818 22.889358128989546 52-53 22.9012883239518 27.252254727166335 26.816510216210226 23.029946732671636 54-55 22.57464125928573 27.41499960967252 26.883215620791244 23.1271435102505 56-57 22.642665769853384 27.594796682004525 26.87285130535096 22.889686242791136 58-59 22.740188593288206 27.040495589645914 26.947091776114725 23.272224040951162 60-61 22.556844717539217 27.187571542385143 27.20918306608914 23.046400673986504 62-63 22.46538630250893 27.228610204756308 27.329642182418922 22.976361310315838 64-65 22.48084535906882 27.187960437093018 27.032905599006625 23.298288604831537 66-67 22.47794932752489 27.41101317934913 26.829749073581 23.28128841954498 68-69 22.539349956191973 27.151269448325642 27.182497119338926 23.126883476143455 70-71 22.54926379019852 27.04021225551242 27.0587319610285 23.35179199326057 72-73 22.47716472374793 27.06651895310186 27.008344717488647 23.44797160566156 74-75 22.531601404145633 27.316392711135247 26.87540508383504 23.276600800884083 76-77 22.67945466640966 27.08894292512674 27.049087257128807 23.182515151334787 78-79 22.756967978057585 27.004247325132937 27.09609375661749 23.142690940191994 80-81 22.667753989661886 27.297103377035352 26.96696867200224 23.06817396130052 82-83 22.73871926753798 27.14960958025155 26.98535968871738 23.126311463493092 84-85 22.71308664677313 27.02223347599474 27.086795127564205 23.17788474966792 86-87 22.781261726417064 26.997860941263685 27.104024008375827 23.116853323943424 88-89 22.703259231344592 26.99355672374868 27.144501968836092 23.158682076070633 90-91 22.685438184600837 27.20227840653485 27.06452547124347 23.047757937620837 92-93 22.80047526602077 26.758209556601525 27.184460907309386 23.256854270068313 94-95 22.561334637312484 26.707718201314602 27.407645493488346 23.323301667884568 96-97 22.814843405886485 26.613375859754385 27.326201641534503 23.245579092824627 98-99 22.739658604313632 26.675770246403303 27.08705670126103 23.49751444802203 100-101 22.543725380935875 26.607682407147525 27.419713749222197 23.428878462694406 102-103 22.547944051384523 26.406888448922256 27.466272955544035 23.578894544149186 104-105 22.320338879447842 26.696852572193087 27.367335898479023 23.615472649880047 106-107 22.5217144863816 26.619320822236293 27.162120027965226 23.696844663416876 108-109 22.591379238116996 26.677442638096988 27.005986913627332 23.725191210158684 110-111 22.496833956469306 26.75160270938239 27.24097734825197 23.51058598589634 112-113 22.450637712586126 26.779990989242037 27.41610105235397 23.35327024581787 114-115 22.37894640445065 26.928455321779342 27.16296160626388 23.529636667506125 116-117 22.335528695539097 26.93287722790955 27.298491115834356 23.433102960716997 118-119 22.26372399187175 26.96056862879142 27.078895317814293 23.696812061522536 120-121 22.193410266978063 27.046091496084628 27.23883775243852 23.521660484498785 122-123 22.33810879854412 27.129433966670735 27.232745026609695 23.29971220817545 124-125 22.310835222267883 27.155800331185926 27.108304497396563 23.425059949149627 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 869.0 1 682.0 2 405.5 3 296.0 4 253.0 5 217.5 6 192.5 7 185.0 8 200.0 9 196.0 10 171.5 11 161.5 12 165.0 13 172.5 14 187.0 15 225.5 16 257.5 17 296.5 18 403.5 19 523.5 20 693.5 21 973.0 22 1393.0 23 2094.0 24 3042.0 25 4456.0 26 6672.5 27 9704.0 28 13118.5 29 17357.5 30 22427.0 31 27293.5 32 33106.5 33 38049.5 34 42464.0 35 47779.0 36 56202.0 37 64548.5 38 68500.0 39 71807.5 40 72870.5 41 74522.0 42 76462.5 43 77879.0 44 80553.0 45 83192.0 46 83531.0 47 81700.5 48 81233.5 49 81862.5 50 81842.5 51 79971.5 52 78316.0 53 77461.5 54 77275.0 55 72571.5 56 63859.0 57 57696.0 58 52794.0 59 46175.5 60 37706.0 61 30669.5 62 25066.5 63 18793.5 64 13463.0 65 9992.5 66 7409.0 67 5770.0 68 4471.0 69 3228.5 70 2322.0 71 1675.5 72 1322.5 73 1049.0 74 758.5 75 531.0 76 352.5 77 213.0 78 131.5 79 84.5 80 41.5 81 20.0 82 18.5 83 16.5 84 12.0 85 9.0 86 5.5 87 6.5 88 5.0 89 3.5 90 5.0 91 6.0 92 5.5 93 3.5 94 1.5 95 1.0 96 2.0 97 1.5 98 0.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09151866037557903 2 3.662577703875099E-4 3 0.0 4 0.0019228532945344268 5 0.0010987733111625297 6 4.1203999168594864E-4 7 1.373466638953162E-4 8 0.0012819021963562847 9 6.867333194765811E-4 10-11 0.009316682034232282 12-13 0.001991526626482085 14-15 0.0042119643594563634 16-17 0.0024493488394664725 18-19 0.01341419084044255 20-21 0.016596055220684042 22-23 0.010072088685656523 24-25 0.010209435349551838 26-27 0.0101636531282534 28-29 0.007691413178137707 30-31 0.01966346404767944 32-33 0.002312002175571156 34-35 0.0015337044134976976 36-37 5.951688768797035E-4 38-39 2.9758443843985174E-4 40-41 0.0016252688560945752 42-43 0.0027240421672571047 44-45 8.469710940211166E-4 46-47 0.001556595524146917 48-49 0.008767295378651018 50-51 0.004944479900231383 52-53 0.008057670948525218 54-55 0.008721513157352579 56-57 0.010278108681499497 58-59 0.008652839825404922 60-61 0.010713039783834664 62-63 0.01336840861914411 64-65 0.008607057604106481 66-67 0.01405514193862069 68-69 0.013437081951091768 70-71 0.004303528802053241 72-73 0.013620210836285524 74-75 0.01339129972979333 76-77 0.005631213219707964 78-79 0.007943215395279122 80-81 0.013597319725636305 82-83 0.01769482853184657 84-85 0.013505755283039426 86-87 0.016000886343804338 88-89 0.016000886343804338 90-91 0.012429873082526115 92-93 0.00979739535786589 94-95 0.007782977620734585 96-97 0.009156444259687747 98-99 0.006020362100744693 100-101 0.010300999792148715 102-103 0.011445555324609683 104-105 0.010644366451887006 106-107 0.005173391006723577 108-109 0.0033649932654352473 110-111 0.006409510981781423 112-113 0.00906487981709087 114-115 0.013917795274725375 116-117 0.011720248652400317 118-119 0.21494752899616984 120-121 0.02536335059933506 122-123 0.025523588373879594 124-125 0.23419895305216337 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2184254.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.5881127192196 #Duplication Level Percentage of deduplicated Percentage of total 1 84.64891099724534 54.67313405049269 2 8.907892321616298 11.506879067184483 3 2.7770340605061143 5.38090166775243 4 1.2574585952438853 3.2486750995745437 5 0.6439528769532773 2.0795850501262008 6 0.38118803108891064 1.4772129311512765 7 0.23261924618084565 1.0517106665091847 8 0.17757159081236584 0.9175211138496179 9 0.12921191728482398 0.7510998490432821 >10 0.7057181143511279 8.750237955323483 >50 0.08646419483196381 3.8559819359066236 >100 0.050402961381759294 5.704918716219302 >500 0.0015750925032901595 0.6021418968668418 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 2.289111064921937E-5 0.0 0.0 4.578222129843874E-5 0.0 20-21 4.578222129843874E-5 0.0 0.0 1.1445555324609684E-4 0.0 22-23 4.578222129843874E-5 0.0 0.0 2.975844384398518E-4 0.0 24-25 4.578222129843874E-5 0.0 0.0 4.578222129843873E-4 0.0 26-27 4.578222129843874E-5 0.0 0.0 8.927533153195554E-4 0.0 28-29 4.578222129843874E-5 0.0 0.0 0.003571013261278221 0.0 30-31 4.578222129843874E-5 0.0 0.0 0.013643101946934744 0.0 32-33 4.578222129843874E-5 0.0 0.0 0.034931834850708755 0.0 34-35 6.86733319476581E-5 0.0 0.0 0.06530833868222286 0.0 36-37 9.156444259687748E-5 0.0 0.0 0.11452422657804451 0.0 38-39 9.156444259687748E-5 0.0 0.0 0.18837094953242617 0.0 40-41 9.156444259687748E-5 0.0 0.0 0.27025245232468387 0.0 42-43 9.156444259687748E-5 0.0 0.0 0.35101229069512974 0.0 44-45 9.156444259687748E-5 0.0 0.0 0.4505199486872864 0.0 46-47 9.156444259687748E-5 0.0 0.0 0.5710187551447772 0.0 48-49 9.156444259687748E-5 0.0 0.0 0.7001704014276728 0.0 50-51 9.156444259687748E-5 0.0 0.0 0.8410422963629688 0.0 52-53 9.156444259687748E-5 0.0 0.0 0.9741541047881793 0.0 54-55 9.156444259687748E-5 0.0 0.0 1.119787350738513 0.0 56-57 9.156444259687748E-5 0.0 0.0 1.2940573761110201 0.0 58-59 9.156444259687748E-5 0.0 0.0 1.487739063314065 0.0 60-61 9.156444259687748E-5 0.0 0.0 1.699664965704538 0.0 62-63 9.156444259687748E-5 0.0 0.0 1.9062572393137427 0.0 64-65 9.156444259687748E-5 0.0 0.0 2.1189156572449908 0.0 66-67 9.156444259687748E-5 0.0 0.0 2.359340992393742 0.0 68-69 9.156444259687748E-5 0.0 0.0 2.635865609036312 0.0 70-71 9.156444259687748E-5 0.0 0.0 2.9424462539613065 0.0 72-73 9.156444259687748E-5 0.0 0.0 3.2471269367024167 0.0 74-75 9.156444259687748E-5 0.0 0.0 3.5540280571765006 0.0 76-77 9.156444259687748E-5 0.0 0.0 3.8796998883829446 0.0 78-79 9.156444259687748E-5 0.0 0.0 4.246438372094088 0.0 80-81 9.156444259687748E-5 0.0 0.0 4.6501688906143706 0.0 82-83 9.156444259687748E-5 0.0 0.0 5.071594237666499 0.0 84-85 1.1445555324609684E-4 0.0 0.0 5.484023378233484 0.0 86-87 1.373466638953162E-4 0.0 0.0 5.9089739563255925 0.0 88-89 1.373466638953162E-4 0.0 0.0 6.3772116246553745 0.0 90-91 1.6023777454453558E-4 0.0 0.0 6.8926507631438465 0.0 92-93 1.8312888519375495E-4 0.0 0.0 7.424365481303914 0.0 94-95 1.8312888519375495E-4 0.0 0.0 7.967067932575608 0.0 96-97 1.8312888519375495E-4 0.0 0.0 8.509541472740807 0.0 98-99 2.2891110649219366E-4 0.0 0.0 9.073463983584325 0.0 100-101 2.2891110649219366E-4 0.0 0.0 9.705235746392132 0.0 102-103 2.2891110649219366E-4 0.0 0.0 10.368139419682876 0.0 104-105 3.662577703875099E-4 0.0 0.0 11.033881590694122 0.0 106-107 3.662577703875099E-4 0.0 0.0 11.684080697574549 0.0 108-109 3.891488810367292E-4 0.0 0.0 12.361520226127547 0.0 110-111 4.120399916859486E-4 0.0 0.0 13.088038295912472 0.0 112-113 4.120399916859486E-4 0.0 0.0 13.850380953863425 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1905 0.0 50.31075 1 GTATCAA 3295 0.0 48.237606 1 ATCAACG 3510 0.0 43.88687 3 CAACGCA 3635 0.0 42.709824 5 TCAACGC 3745 0.0 41.450584 4 AACGCAG 3795 0.0 40.593807 6 GTACATG 10505 0.0 39.327225 1 TACATGG 10820 0.0 37.43362 2 TATCAAC 4285 0.0 36.782143 2 ACATGGG 10590 0.0 36.112453 3 ACGCAGA 4935 0.0 31.337042 7 CGCAGAG 5040 0.0 30.56617 8 CATGGGA 7170 0.0 29.862444 4 CATGGGG 4450 0.0 29.136572 4 ATGGGAT 3260 0.0 26.457088 5 GAGTACA 7550 0.0 23.81167 1 AGTACAT 7765 0.0 22.212555 2 GCAGAGT 6700 0.0 22.105299 9 TAAGGTG 990 0.0 21.630125 5 GTACTAG 415 1.8189894E-12 21.516573 1 >>END_MODULE