FastQCFastQC Report
Thu 26 May 2016
SRR1364449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364449_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184254
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28190.1290600818402988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26500.12132288644086264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29850.071.2725451
GTATCAA54300.050.374561
ATCAACG65250.041.1675953
CAACGCA67450.039.874775
TCAACGC67500.039.7944344
AACGCAG70150.038.4213336
TATCAAC70450.038.0452962
GTACATG103550.037.695511
TACATGG105750.036.5539052
ACATGGG105300.036.143653
CATGGGA69950.031.588994
ACGCAGA86300.031.3691357
CGCAGAG88050.030.5415538
CATGGGG44350.027.664524
CGTCTAG900.00945818226.4859711
ATGGGAT32850.026.2648145
GCAGAGT105600.025.1277149
GAGTACT51000.024.78967312-13
CGTATAC1451.3313742E-424.645793
CAGAGTA100550.023.4608210-11
Produced by FastQC (version 0.11.5)