FastQCFastQC Report
Thu 26 May 2016
SRR1364448_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364448_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886684
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.054.997271
GTATCAA28250.045.9322971
ATCAACG29500.041.531723
TCAACGC31000.039.9068874
GTACATG80750.039.0673071
CAACGCA32300.038.118615
AACGCAG32650.037.5278136
TACATGG87750.035.042062
ACATGGG85950.033.975853
TATCAAC37450.033.6692
ACGCAGA39700.030.7137327
CGCAGAG40600.029.8863838
CATGGGA55250.028.419594
CATGGGG39150.026.7378884
GCAGAGT50850.023.277859
GAGTACA60900.023.0661331
GGACCGA4150.022.9319576
ATGGGAT26500.021.7718375
AGTACAT61800.021.6541062
ATGGGAG25000.021.6506335
Produced by FastQC (version 0.11.5)