FastQCFastQC Report
Thu 26 May 2016
SRR1364448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364448_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886684
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29830.15810808805290127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28150.14920357622156122No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29400.067.883681
GTATCAA51700.051.970141
ATCAACG62900.042.4164163
TCAACGC64900.041.109284
CAACGCA66250.039.9669235
TATCAAC67600.039.4673422
AACGCAG67400.039.459466
GTACATG85650.033.5264471
ACGCAGA80450.033.058647
CGCAGAG81500.032.5580028
TACATGG91300.030.9834232
ACATGGG89750.030.788613
GTGGTAT15500.030.3642981
TGGTATC15600.028.6314432
CATGGGA53800.028.2269844
GCAGAGT95050.027.41599
GAGTACT51350.025.77923812-13
CAGAGTA90200.025.06441310-11
GTACTTT54900.024.11227214-15
AGAGTAC88850.024.03906210-11
Produced by FastQC (version 0.11.5)