##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364448_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1886684 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89924226844559 31.0 31.0 34.0 30.0 34.0 2 31.841601985282114 33.0 31.0 34.0 30.0 34.0 3 32.07133945059162 33.0 31.0 34.0 30.0 34.0 4 35.766534300391584 37.0 35.0 37.0 35.0 37.0 5 35.70188807452652 37.0 35.0 37.0 33.0 37.0 6 35.7670039073846 37.0 35.0 37.0 33.0 37.0 7 35.68012184340356 37.0 35.0 37.0 33.0 37.0 8 35.69387772409158 37.0 35.0 37.0 33.0 37.0 9 37.332190764325134 39.0 37.0 39.0 34.0 39.0 10-11 37.291056689938536 39.0 37.0 39.0 33.5 39.0 12-13 37.253788127741586 39.0 37.0 39.0 33.0 39.0 14-15 38.555984467987216 40.0 38.0 41.0 34.0 41.0 16-17 38.56917878139635 40.0 38.0 41.0 34.0 41.0 18-19 38.62426140254542 40.0 38.0 41.0 34.0 41.0 20-21 38.628682651678815 40.0 38.0 41.0 34.0 41.0 22-23 38.598408371513194 40.0 38.0 41.0 34.0 41.0 24-25 38.54547475888914 40.0 38.0 41.0 34.0 41.0 26-27 38.42888607737173 40.0 38.0 41.0 34.0 41.0 28-29 38.353011686111714 40.0 38.0 41.0 34.0 41.0 30-31 38.23928145889826 40.0 38.0 41.0 33.0 41.0 32-33 38.11181045686506 40.0 37.0 41.0 33.0 41.0 34-35 38.0142541093262 40.0 37.0 41.0 33.0 41.0 36-37 37.91642267597541 40.0 37.0 41.0 33.0 41.0 38-39 37.817087546192155 40.0 37.0 41.0 33.0 41.0 40-41 37.703070307481276 40.0 37.0 41.0 33.0 41.0 42-43 37.56743498116272 40.0 37.0 41.0 32.0 41.0 44-45 37.39664723928331 40.0 37.0 41.0 32.0 41.0 46-47 37.17987034394737 40.0 36.0 41.0 31.0 41.0 48-49 36.95857546891796 40.0 36.0 41.0 31.0 41.0 50-51 36.74351375217047 39.5 36.0 41.0 30.5 41.0 52-53 36.61434638763036 39.0 35.0 41.0 30.0 41.0 54-55 36.45165088589292 39.0 35.0 41.0 30.0 41.0 56-57 36.23420959736766 39.0 35.0 41.0 30.0 41.0 58-59 36.009030394067054 38.5 35.0 40.0 29.0 41.0 60-61 35.76805124758572 38.0 35.0 40.0 29.0 41.0 62-63 35.80718843219108 38.0 35.0 40.0 29.0 41.0 64-65 35.5883600539359 37.5 35.0 40.0 29.0 41.0 66-67 35.31125111571413 37.0 34.0 40.0 29.0 41.0 68-69 34.98956078495392 36.5 34.0 39.0 29.0 41.0 70-71 34.66161026435799 36.0 34.0 39.0 29.0 41.0 72-73 34.31716228048788 35.5 34.0 39.0 29.0 40.5 74-75 33.97915761197954 35.0 34.0 37.5 28.0 40.0 76-77 33.66542860383615 35.0 34.0 37.0 28.0 39.0 78-79 33.34810572411702 35.0 33.0 36.5 28.0 39.0 80-81 33.072118595376864 35.0 33.0 36.0 27.5 38.5 82-83 32.8128645284531 35.0 33.0 36.0 27.0 37.0 84-85 32.59437086443729 35.0 33.0 35.0 27.0 37.0 86-87 32.41399593148614 35.0 33.0 35.0 27.0 36.5 88-89 32.2421847007766 35.0 33.0 35.0 26.0 36.0 90-91 32.120908959847014 35.0 33.0 35.0 26.0 36.0 92-93 31.99706866650695 35.0 33.0 35.0 26.0 36.0 94-95 31.88982707226011 35.0 33.0 35.0 26.0 35.0 96-97 31.841634052125315 35.0 33.0 35.0 25.5 35.0 98-99 31.764723716319214 35.0 33.0 35.0 25.0 35.0 100-101 31.75947031935396 35.0 33.0 35.0 25.0 35.0 102-103 30.617974446171168 33.5 30.5 34.5 24.0 35.0 104-105 31.597512620025398 35.0 32.5 35.0 25.0 35.0 106-107 31.789186212423488 35.0 33.0 35.0 25.0 35.0 108-109 31.79589851824683 35.0 33.0 35.0 25.0 35.0 110-111 31.736282811536007 35.0 33.0 35.0 25.0 35.0 112-113 31.682176506505595 35.0 33.0 35.0 25.0 35.0 114-115 31.574571311358977 35.0 33.0 35.0 25.0 35.0 116-117 31.490598584606644 35.0 33.0 35.0 25.0 35.0 118-119 31.390839695465694 35.0 33.0 35.0 25.0 35.0 120-121 31.26991377464377 35.0 33.0 35.0 24.5 35.0 122-123 31.0919231837446 35.0 33.0 35.0 24.0 35.0 124-125 30.081971066696912 34.5 31.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28816147014902427 1101 2 0.45427619700257793 1101 3 0.3246902798216311 1101 4 0.16340014669399494 1101 5 0.26645923741702404 1101 6 0.2126554861353469 1101 7 0.3158864238572363 1101 8 0.2779486238590252 1101 9 0.4259497539465471 1101 10-11 0.3762905016666309 1101 12-13 0.3742928328421442 1101 14-15 0.40352813866047654 1101 16-17 0.394251947770897 1101 18-19 0.31829935708412194 1101 20-21 0.3167801306978788 1101 22-23 0.3518839392017199 1101 24-25 0.46433730666415585 1101 26-27 0.3275982364058052 1101 28-29 0.3124956780349706 1101 30-31 0.32115630099990256 1101 32-33 0.361387103867699 1101 34-35 0.3649904326096589 1101 36-37 0.2555103940914236 1101 38-39 0.18033802012003264 1101 40-41 0.22325269626878708 1101 42-43 0.14322029581579443 1101 44-45 0.19383627400343784 1101 46-47 0.20735242509213947 1101 48-49 0.1197488024170994 1101 50-51 0.22627535775117025 1101 52-53 0.24667348736737438 1101 54-55 0.16472358876780646 1101 56-57 0.17578529131488096 1101 58-59 0.0016853493125026375 1101 60-61 -0.004587302696961615 1101 62-63 0.25315982610023724 1101 64-65 0.13939758562658255 1101 66-67 0.27266705581789097 1101 68-69 0.3494979819599138 1101 70-71 0.3169005770819737 1101 72-73 0.3129095482373714 1101 74-75 0.30729533401596854 1101 76-77 0.3207717734624538 1101 78-79 0.34225049890891057 1101 80-81 0.24057863868890195 1101 82-83 0.29031367116485285 1101 84-85 0.21272790539530462 1101 86-87 0.2925287224879014 1101 88-89 0.2638268830081003 1101 90-91 0.2152644661658769 1101 92-93 0.2931913885574744 1101 94-95 0.24809938731536718 1101 96-97 0.13727598979059152 1101 98-99 0.18316904978792792 1101 100-101 0.14916052496732135 1101 102-103 0.17785403707726033 1101 104-105 0.3026241172565598 1101 106-107 0.2516692053392511 1101 108-109 0.22439794425586612 1101 110-111 0.22155089136570538 1101 112-113 0.21827099733316047 1101 114-115 0.27056028659908193 1101 116-117 0.2677519599888889 1101 118-119 0.23067983404712322 1101 120-121 0.24598115774929497 1101 122-123 0.23197293048614043 1101 124-125 -0.0866474121797971 1102 1 0.3511542262770071 1102 2 0.5110049575790825 1102 3 0.30069645665025746 1102 4 0.29184875077079653 1102 5 0.24247142575769232 1102 6 0.27985116586201997 1102 7 0.29875336404770536 1102 8 0.32490028629258205 1102 9 0.3240392202745639 1102 10-11 0.3376195718360009 1102 12-13 0.40757776097953524 1102 14-15 0.3657868014998158 1102 16-17 0.4335137591396716 1102 18-19 0.3931684188373836 1102 20-21 0.4137214608451032 1102 22-23 0.3853028739617841 1102 24-25 0.3558581941637158 1102 26-27 0.3814973281665246 1102 28-29 0.3976170604823608 1102 30-31 0.4682804186852749 1102 32-33 0.43076089217230873 1102 34-35 0.34639194149918495 1102 36-37 0.3885640042548886 1102 38-39 0.3669717988062189 1102 40-41 0.29970435118795535 1102 42-43 0.5081577760905276 1102 44-45 0.5140487308583204 1102 46-47 0.3605243117550998 1102 48-49 0.3493649548490083 1102 50-51 0.35641044143643086 1102 52-53 0.3084223614612611 1102 54-55 0.21465019822491627 1102 56-57 0.2225851630692972 1102 58-59 0.17415531680024543 1102 60-61 0.14795206018295204 1102 62-63 0.2561685252591275 1102 64-65 0.31541490880046297 1102 66-67 0.2379465660800406 1102 68-69 0.14114661409044515 1102 70-71 0.11880178038629907 1102 72-73 0.15300357830495415 1102 74-75 0.1873104379399777 1102 76-77 0.2756256937197463 1102 78-79 0.2043186719603085 1102 80-81 0.16010089881704914 1102 82-83 0.17296776625695998 1102 84-85 -0.003159143302951861 1102 86-87 0.15195233119858642 1102 88-89 0.18408129520745575 1102 90-91 0.16575653347477726 1102 92-93 0.20087385047204975 1102 94-95 0.26583998556100497 1102 96-97 0.2903495631836428 1102 98-99 0.1730222436541844 1102 100-101 0.12152886032954058 1102 102-103 0.18783527615008566 1102 104-105 0.09341867633719758 1102 106-107 0.007803452422415091 1102 108-109 0.08398261139344143 1102 110-111 0.07971984741654126 1102 112-113 0.16091672640433075 1102 114-115 0.23031646968970065 1102 116-117 0.20241064386851804 1102 118-119 0.11411886980059194 1102 120-121 0.08018691176824078 1102 122-123 0.06030744107391328 1102 124-125 -0.022877167160586964 1103 1 0.3691389417943789 1103 2 0.5271604706348967 1103 3 0.3361711855858971 1103 4 0.2922288063974747 1103 5 0.2751668774288092 1103 6 0.2758092350714776 1103 7 0.32627442969437936 1103 8 0.24869868329840727 1103 9 0.3472138736360719 1103 10-11 0.32099133487253795 1103 12-13 0.2650718744229863 1103 14-15 0.43342918419282483 1103 16-17 0.47250139994147133 1103 18-19 0.2782589150046988 1103 20-21 0.3656050946755869 1103 22-23 0.3337777031488187 1103 24-25 0.44813742258781986 1103 26-27 0.37992402382898405 1103 28-29 0.4396222684140483 1103 30-31 0.5244935634431513 1103 32-33 0.4505962099692482 1103 34-35 0.384627439748634 1103 36-37 0.3111568781797587 1103 38-39 0.30069367057696894 1103 40-41 0.2754761584379324 1103 42-43 0.37475825112792904 1103 44-45 0.4266421060401768 1103 46-47 0.35177474232200723 1103 48-49 0.3613739800455207 1103 50-51 0.3122328627904878 1103 52-53 0.2875270136403216 1103 54-55 0.2569789097116697 1103 56-57 0.315797629202315 1103 58-59 0.14263749775658852 1103 60-61 0.12679464492109105 1103 62-63 0.3243856826435447 1103 64-65 0.4465301156525072 1103 66-67 0.42918202320072396 1103 68-69 0.46423849578351195 1103 70-71 0.5211384734267952 1103 72-73 0.5058825928594004 1103 74-75 0.37406375780363277 1103 76-77 0.4650688357528452 1103 78-79 0.6147163486983871 1103 80-81 0.5558686188945785 1103 82-83 0.47860838555354945 1103 84-85 0.5149846891795278 1103 86-87 0.5816481453170681 1103 88-89 0.5505527286996497 1103 90-91 0.4295301760965273 1103 92-93 0.4412070449957497 1103 94-95 0.46890060938176603 1103 96-97 0.42977091981642346 1103 98-99 0.41033491491699436 1103 100-101 0.37662882896884753 1103 102-103 0.3820259645944404 1103 104-105 0.4588408761099636 1103 106-107 0.48767477075402255 1103 108-109 0.425249236417212 1103 110-111 0.42367937926820787 1103 112-113 0.4441161984136315 1103 114-115 0.3489758257158364 1103 116-117 0.3527130046604512 1103 118-119 0.3703699301519343 1103 120-121 0.28857127049345266 1103 122-123 0.3123140113964169 1103 124-125 0.22167776803587813 1104 1 0.23426358948592352 1104 2 0.3862284439667292 1104 3 0.2302735739661017 1104 4 0.15876647682099332 1104 5 0.20164162370278405 1104 6 0.23292966666502224 1104 7 0.18738071258972155 1104 8 0.19497729718633394 1104 9 0.1766151185737499 1104 10-11 0.2588478762418731 1104 12-13 0.24005560583547947 1104 14-15 0.26723390004666925 1104 16-17 0.23195322432029997 1104 18-19 0.2515731033442563 1104 20-21 0.24080418950747884 1104 22-23 0.174522558178424 1104 24-25 0.22236039032864596 1104 26-27 0.1838202321351048 1104 28-29 0.1733683036858551 1104 30-31 0.12444177392220723 1104 32-33 0.14576858358694267 1104 34-35 0.10061206107891252 1104 36-37 0.07636682347278168 1104 38-39 -0.02483466778691934 1104 40-41 0.028109858047344005 1104 42-43 0.08986236771994527 1104 44-45 0.06491752209687718 1104 46-47 0.17438941691946752 1104 48-49 0.10200639948049428 1104 50-51 0.16009990177968803 1104 52-53 0.09726884276000192 1104 54-55 0.0710582592144462 1104 56-57 0.179215259236166 1104 58-59 -0.12888037329863522 1104 60-61 -0.01879393931396578 1104 62-63 0.26074685191689895 1104 64-65 0.20291660187915284 1104 66-67 0.2509208699828349 1104 68-69 0.2887380887136146 1104 70-71 0.25573387341855636 1104 72-73 0.13066171185500508 1104 74-75 0.08561832671922076 1104 76-77 0.1608596288216262 1104 78-79 0.20336546865542005 1104 80-81 0.1489783392690498 1104 82-83 0.26222131204747967 1104 84-85 0.20231143582670086 1104 86-87 0.18007315896895193 1104 88-89 0.266635328604643 1104 90-91 0.15718229923007954 1104 92-93 0.17400372411999854 1104 94-95 0.25706548429361575 1104 96-97 0.26952687205924164 1104 98-99 0.24609890625746544 1104 100-101 0.15073688649002293 1104 102-103 0.11830730649005616 1104 104-105 0.13030142821893165 1104 106-107 0.18087721260507195 1104 108-109 0.1507791613827969 1104 110-111 0.3469053217688156 1104 112-113 0.09795185536855655 1104 114-115 0.08412251059448295 1104 116-117 -0.04832052425903299 1104 118-119 -0.13939015672753285 1104 120-121 -0.14019291834340564 1104 122-123 -0.24922991307944997 1104 124-125 -0.2574895005132696 1105 1 0.1772608356983021 1105 2 0.39931450586474426 1105 3 0.22620138499276266 1105 4 0.1883167719757708 1105 5 0.1156270767221983 1105 6 0.10957743624560834 1105 7 0.12563449927981196 1105 8 0.07156332473164184 1105 9 0.18355575894542397 1105 10-11 0.1448170683589467 1105 12-13 0.1775236903097266 1105 14-15 0.2099078129690426 1105 16-17 0.24446596731930725 1105 18-19 0.16843675650443402 1105 20-21 0.2241558907094543 1105 22-23 0.26463482655294257 1105 24-25 0.3058103217160735 1105 26-27 0.25692767627481317 1105 28-29 0.22160972339985818 1105 30-31 0.09426709502721309 1105 32-33 0.08215246863720438 1105 34-35 0.18045797524170126 1105 36-37 0.1007263775188818 1105 38-39 0.08939575720254567 1105 40-41 0.0077751419400016175 1105 42-43 0.03268761826262789 1105 44-45 0.16965081507350988 1105 46-47 0.14136903369175968 1105 48-49 0.08538897170010529 1105 50-51 0.114621322087892 1105 52-53 0.1596171569703202 1105 54-55 0.10129630493512565 1105 56-57 -0.12200625818808675 1105 58-59 -0.043275041386444 1105 60-61 0.06137902194546996 1105 62-63 0.19226611450344677 1105 64-65 0.20102697064157837 1105 66-67 0.25334436092393275 1105 68-69 0.23184071418921093 1105 70-71 0.1992761207543623 1105 72-73 0.20549143382086044 1105 74-75 0.3535905975819915 1105 76-77 0.21058517851142966 1105 78-79 0.20603174970516136 1105 80-81 0.19654291772414467 1105 82-83 0.2625590363381889 1105 84-85 0.14477528656139071 1105 86-87 0.21393077347953238 1105 88-89 0.16809643297932553 1105 90-91 0.07370033626044403 1105 92-93 0.17510486778675727 1105 94-95 0.0449242612256775 1105 96-97 0.23708491421466604 1105 98-99 0.1630607431805018 1105 100-101 0.13979345890646755 1105 102-103 0.15496907712164543 1105 104-105 0.1372826883506626 1105 106-107 0.01931803588172798 1105 108-109 0.07314806865090162 1105 110-111 0.1640038267759607 1105 112-113 0.21372583123995526 1105 114-115 0.17593941621365872 1105 116-117 0.16304613801295886 1105 118-119 0.09455969945530285 1105 120-121 0.1754818060457204 1105 122-123 0.1672630557561412 1105 124-125 0.035156837394854534 1106 1 0.15486947930623174 1106 2 0.2499898708601478 1106 3 0.20314359373745106 1106 4 0.056760249939245 1106 5 0.07268644120079415 1106 6 0.01010478905043044 1106 7 0.07249118775879992 1106 8 -0.017078212163106343 1106 9 0.12352247045275533 1106 10-11 0.18563869751906026 1106 12-13 0.1346046676081869 1106 14-15 0.21013819137864687 1106 16-17 0.15248798104511252 1106 18-19 0.15746219408090667 1106 20-21 0.16012945561821113 1106 22-23 0.17921027476958074 1106 24-25 0.09935872873249707 1106 26-27 0.08827001764146303 1106 28-29 -0.013452968027969803 1106 30-31 -0.027884050309737063 1106 32-33 0.05407551559829926 1106 34-35 0.07317383743646388 1106 36-37 0.10016563171984671 1106 38-39 0.03744281329898058 1106 40-41 -0.09659079017578875 1106 42-43 -0.005491546789016866 1106 44-45 0.03674266053282338 1106 46-47 0.12083257975224626 1106 48-49 0.11345381205974547 1106 50-51 0.1046122114530661 1106 52-53 0.07279632202196495 1106 54-55 0.006496539398597179 1106 56-57 0.008258719733589714 1106 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2216 120-121 -0.7251283310570606 2216 122-123 -0.898682320586417 2216 124-125 -0.7257005719865752 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 77.0 3 14.0 4 38.0 5 94.0 6 322.0 7 731.0 8 1252.0 9 2168.0 10 3300.0 11 4401.0 12 5777.0 13 7672.0 14 11100.0 15 8774.0 16 5126.0 17 4737.0 18 4761.0 19 4764.0 20 4800.0 21 4970.0 22 5425.0 23 6532.0 24 8568.0 25 11845.0 26 15460.0 27 21257.0 28 27336.0 29 34624.0 30 43396.0 31 53972.0 32 68074.0 33 87420.0 34 115546.0 35 168808.0 36 316742.0 37 582584.0 38 243523.0 39 694.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.307139641419155 18.928711761390122 12.702835844085051 25.061312753105675 2 15.97130272078194 20.491256711845182 38.25796807997498 25.279472487397896 3 19.019650973273087 24.741251142883645 29.54388010653663 26.695217777306635 4 12.952806633286635 16.326580410458746 36.47734753408068 34.24326542217394 5 12.966900159814266 38.65856729028685 34.615557905505504 13.758974644393382 6 32.99805372812829 36.694115177740414 16.60770961114845 13.70012148298284 7 28.890427245592935 32.38522972606944 20.928477191182107 17.79586583715552 8 26.82166170911504 35.3209122460359 19.336677472221105 18.520748572627955 9 26.82176771520827 15.004738472367391 19.393920762565433 38.779573049858904 10-11 25.154742394592844 26.39776984381062 27.551699171668385 20.89578858992815 12-13 25.83718312128581 23.649614879863297 28.02692448761955 22.486277511231346 14-15 22.6445711099474 25.162825359201648 25.511744415068975 26.680859115781974 16-17 22.491259797613168 28.624692847344868 26.27480277566355 22.609244579378423 18-19 22.535732713762233 26.899797342582733 28.29160618790188 22.272863755753157 20-21 23.01596345757954 26.249626328521362 28.559393093915038 22.175017119984055 22-23 22.94409132636944 26.181649921237472 28.351674154230384 22.522584598162705 24-25 22.726778835247448 26.39496068234002 28.339615961125446 22.538644521287083 26-27 22.476911774994754 26.754169767358043 28.187007202084512 22.58191125556269 28-29 22.51267177725022 26.61189829928133 28.394586661281522 22.480843262186927 30-31 22.57937393430015 26.325363563652594 28.359502001143817 22.735760500903442 32-33 22.44591569123393 26.479315031027983 28.317487189163632 22.757282088574453 34-35 22.930231029679586 26.400207983954914 28.085413349559335 22.584147636806165 36-37 23.018560606863684 26.163549380818406 27.945087783645807 22.872802228672104 38-39 22.721782090579072 26.366432516750297 28.138245799638838 22.773539593031792 40-41 22.82544515127776 26.21672562440967 28.200361272978412 22.757467951334156 42-43 22.682041543014122 26.08099133341213 28.098168679117876 23.13879844445587 44-45 22.704544057192408 26.31643666877972 28.048549730638516 22.930469543389354 46-47 23.119955435038406 26.586169173004066 27.421523689181655 22.872351702775877 48-49 23.100874338256965 26.612140665845473 27.24314193579847 23.043843060099096 50-51 23.005362848256517 26.75705099529121 27.269298939303035 22.968287217149243 52-53 23.22047571294398 26.559005111613814 27.1591056053902 23.061413570052007 54-55 22.9285349321879 26.559879661882963 27.29611848089028 23.215466925038854 56-57 22.912902328169928 26.82271721414658 27.241072256747984 23.023308200935507 58-59 22.933412908763785 26.413945770863574 27.257385263368793 23.395256057003852 60-61 22.727323321358114 26.598924880504 27.593850341924465 23.07990145621342 62-63 22.702929757377397 26.54776259822174 27.730955251964435 23.018352392436427 64-65 22.601850654375614 26.368194143799386 27.521328425957925 23.50862677586708 66-67 22.554669373357733 26.704069179631446 27.40023824888395 23.34102319812687 68-69 22.527089857124988 26.404130209404435 27.746140848175955 23.32263908529462 70-71 22.653025095882512 26.349987597287093 27.438776180854873 23.55821112597552 72-73 22.661691094004084 26.57167283975483 27.097304053036968 23.66933201320412 74-75 22.6424774896061 26.708712216778217 27.05503412336141 23.593776170254266 76-77 22.706823188196857 26.632069811372755 27.195995725834322 23.465111274596065 78-79 22.718059834079263 26.613995772477 27.187223721619517 23.480720671824216 80-81 22.752352805239244 26.87779193548045 26.90874571470368 23.461109544576622 82-83 22.736928918674245 26.747563449947105 26.978418219479256 23.537089411899395 84-85 22.673351764259408 26.673438689255857 27.000149468591456 23.65306007789328 86-87 22.702715452084185 26.562609318783643 27.12658823629182 23.60808699284035 88-89 22.660127504128937 26.680779611212053 26.90829518880745 23.75079769585156 90-91 22.666315432120523 26.831227471983173 26.89300270289824 23.609454392998064 92-93 22.687767252219842 26.448929401023758 26.963776268069132 23.899527078687267 94-95 22.47240649692899 26.410284542761982 27.035961659553625 24.0813473007554 96-97 22.658720924993734 26.256646113252792 27.02237694851505 24.062256013238425 98-99 22.56981273896627 26.33064424220161 26.788579552411836 24.310963466420286 100-101 22.354950976624107 26.261288364535652 27.06310351499858 24.32065714384166 102-103 22.387121773525816 26.090934438447455 27.03943595077026 24.482507837256467 104-105 22.262944925495916 26.262609932747026 26.978338902411096 24.49610623934596 106-107 22.351437283061117 26.223153647329728 26.813884724746035 24.61152434486312 108-109 22.40939145130057 26.252240332181426 26.642419924524464 24.69594829199354 110-111 22.341048643239095 26.406931799388516 26.743418681276744 24.50860087609565 112-113 22.162243532796637 26.425106382076503 27.01357365328878 24.399076431838083 114-115 22.159149485389896 26.62079986854816 26.603149278671278 24.616901367390668 116-117 21.961638494649076 26.54905461065796 26.81653462717372 24.67277226751924 118-119 21.878480858343394 26.6684864634943 26.48660395434784 24.96642872381447 120-121 21.788958184868765 26.84531796307098 26.65267731804152 24.713046534018737 122-123 21.799707685641483 26.934502237410673 26.572223245484434 24.693566831463414 124-125 21.702993363662944 26.98873389462823 26.49073688446602 24.817535857242802 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 51.0 1 57.5 2 128.0 3 185.0 4 132.5 5 120.0 6 282.0 7 636.0 8 1047.0 9 1228.5 10 992.0 11 680.0 12 630.5 13 735.0 14 822.0 15 897.5 16 1040.0 17 1150.5 18 1329.0 19 1527.0 20 1682.0 21 2002.5 22 2468.0 23 3172.0 24 4037.0 25 5193.0 26 7091.0 27 9458.0 28 11709.5 29 14672.5 30 18675.0 31 22585.0 32 27486.0 33 31994.0 34 35439.5 35 39109.5 36 44265.0 37 49834.5 38 53460.0 39 55839.0 40 56908.5 41 58229.5 42 59749.5 43 62097.5 44 65677.0 45 68754.5 46 68672.0 47 68600.5 48 69447.5 49 68595.0 50 67725.0 51 66842.5 52 67516.5 53 68602.5 54 69687.5 55 65968.0 56 58927.5 57 54567.0 58 51615.0 59 46472.5 60 38257.0 61 31089.5 62 25249.5 63 19488.5 64 14500.5 65 10833.5 66 7937.0 67 6241.0 68 4744.5 69 3086.0 70 1959.0 71 1357.5 72 1016.5 73 759.0 74 605.5 75 478.0 76 273.0 77 122.5 78 89.5 79 57.0 80 30.0 81 17.5 82 8.5 83 3.0 84 2.5 85 1.0 86 1.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04563562313561784 2 0.004982286381821227 3 4.770274195360749E-4 4 0.08082964608805715 5 0.006307362547199213 6 0.0 7 0.005883338174278258 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.325076165377986E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.240243729209555E-4 28-29 5.830335127663137E-4 30-31 6.890396059965526E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.300304661511944E-5 40-41 0.0011660670255326275 42-43 6.890396059965526E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.770274195360749E-4 58-59 2.3851370976803746E-4 60-61 5.300304661511944E-4 62-63 4.770274195360749E-4 64-65 0.0 66-67 7.950456992267916E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 2.9151675638315686E-4 92-93 3.445198029982763E-4 94-95 4.240243729209555E-4 96-97 0.002915167563831569 98-99 0.009169527064415662 100-101 0.01796803280252549 102-103 0.009646554483951738 104-105 0.004028231542749078 106-107 0.011024633695944842 108-109 0.01298574642070426 110-111 0.006996402153195765 112-113 0.00466426810213051 114-115 0.003259687366829845 116-117 5.300304661511944E-5 118-119 0.001404580735300665 120-121 5.565319894587542E-4 122-123 8.745502691494706E-4 124-125 0.033312414797602566 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1886684.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.26547135590138 #Duplication Level Percentage of deduplicated Percentage of total 1 83.3113988131649 52.70734915234361 2 10.066066851628241 12.736689281365498 3 2.933153573610303 5.567020301811072 4 1.2513405241545956 3.1666659234952474 5 0.6264569224994896 1.9816546243048798 6 0.385082691590084 1.4617462796667522 7 0.24432691430743017 1.0820220179014748 8 0.15952564743476005 0.8073972222652348 9 0.11645212842864361 0.6630658915892492 >10 0.7660277757740512 9.388811444138009 >50 0.09111097301787074 3.99377947195109 >100 0.04735249459562228 5.090186116282755 >500 0.001136459862617258 0.45979198752305234 >1k 5.68229931308629E-4 0.8938202853621182 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2983 0.15810808805290127 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2815 0.14920357622156122 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.300304661511944E-5 0.0 0.0 0.0 0.0 3 5.300304661511944E-5 0.0 0.0 0.0 0.0 4 5.300304661511944E-5 0.0 0.0 0.0 0.0 5 5.300304661511944E-5 0.0 0.0 0.0 0.0 6 1.0600609323023888E-4 0.0 0.0 0.0 0.0 7 1.0600609323023888E-4 0.0 0.0 0.0 0.0 8 1.0600609323023888E-4 0.0 0.0 0.0 0.0 9 1.0600609323023888E-4 0.0 0.0 0.0 0.0 10-11 1.5900913984535832E-4 0.0 0.0 0.0 0.0 12-13 1.5900913984535832E-4 0.0 0.0 5.300304661511944E-5 0.0 14-15 1.5900913984535832E-4 0.0 0.0 5.300304661511944E-5 0.0 16-17 1.8551066315291805E-4 0.0 0.0 5.300304661511944E-5 0.0 18-19 3.445198029982763E-4 0.0 0.0 5.300304661511944E-5 0.0 20-21 4.770274195360749E-4 0.0 0.0 5.300304661511944E-5 0.0 22-23 5.300304661511944E-4 0.0 0.0 1.325076165377986E-4 0.0 24-25 5.830335127663138E-4 0.0 0.0 5.300304661511944E-4 0.0 26-27 5.830335127663138E-4 0.0 0.0 0.001325076165377986 0.0 28-29 6.890396059965526E-4 0.0 0.0 0.0044522559156700324 0.0 30-31 7.420426526116721E-4 0.0 0.0 0.01415181344623689 0.0 32-33 7.685441759192318E-4 0.0 0.0 0.03333891632091013 0.0 34-35 8.215472225343514E-4 0.0 0.0 0.06463721534713815 0.0 36-37 0.0010070578856872694 0.0 0.0 0.11345302127966315 0.0 38-39 0.0010600609323023888 0.0 0.0 0.19120849066404338 0.0 40-41 0.0010600609323023888 0.0 0.0 0.27140210019271904 0.0 42-43 0.0012720731187628666 0.0 0.0 0.34889255434402366 0.0 44-45 0.0014840853052233442 0.0 0.0 0.4432909803655514 0.0 46-47 0.0016695959683762623 0.0 0.0 0.5595531631158159 0.0 48-49 0.001696097491683822 0.0 0.0 0.68005558959529 0.0 50-51 0.001696097491683822 0.0 0.0 0.8115296467240938 0.0 52-53 0.001696097491683822 0.0 0.0 0.9388429646936106 0.0 54-55 0.0017491005382989414 0.0 0.0 1.0776049407319932 0.0 56-57 0.001987614248066979 0.0 0.0 1.2429479446478582 0.0 58-59 0.002014115771374539 0.0 0.0 1.4226812757197282 0.0 60-61 0.0021201218646047775 0.0 0.0 1.622476259935421 0.0 62-63 0.0021201218646047775 0.0 0.0 1.8221122350112684 0.0 64-65 0.002146623387912337 0.0 0.0 2.0241068456614886 0.0 66-67 0.002279131004450136 0.0 0.0 2.2540075603545695 0.0 68-69 0.0024116386209879345 0.0 0.0 2.513430972012271 0.0 70-71 0.0025176447142181736 0.0 0.0 2.798587362801614 0.0 72-73 0.002544146237525733 0.0 0.0 3.087745483610398 0.0 74-75 0.0025706477608332928 0.0 0.0 3.3739089322854277 0.0 76-77 0.0025971492841408523 0.0 0.0 3.6854608402890996 0.0 78-79 0.002623650807448412 0.0 0.0 4.033399339794052 0.0 80-81 0.0029681706104466884 0.0 0.0 4.4279805203203075 0.0 82-83 0.0029946721337542484 0.0 0.0 4.838197599598025 0.0 84-85 0.003127179750292047 0.0 0.0 5.241709793478929 0.0 86-87 0.003233185843522286 0.0 0.0 5.649435729565735 0.0 88-89 0.003286188890137405 0.0 0.0 6.097761999359723 0.0 90-91 0.0037102132630583607 0.0 0.0 6.592624944081786 0.0 92-93 0.0037632163096734802 0.0 0.0 7.104713878953762 0.0 94-95 0.0037632163096734802 0.0 0.0 7.626025343936769 0.0 96-97 0.0037632163096734802 0.0 0.0 8.146727273883702 0.0 98-99 0.0041607391592868755 0.0 0.0 8.689425468175912 0.0 100-101 0.004399252869054913 0.0 0.0 9.297184902188178 0.0 102-103 0.004611265055515391 0.0 0.0 9.940641888095726 0.0 104-105 0.005512316847972421 0.0 0.0 10.568515978298432 0.0 106-107 0.005777332081048019 0.0 0.0 11.191381280596008 0.0 108-109 0.005830335127663138 0.0 0.0 11.845465377349889 0.0 110-111 0.006015845790816056 0.0 0.0 12.5431179784214 0.0 112-113 0.006042347314123616 0.0 0.0 13.28648570719845 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2940 0.0 67.88368 1 GTATCAA 5170 0.0 51.97014 1 ATCAACG 6290 0.0 42.416416 3 TCAACGC 6490 0.0 41.10928 4 CAACGCA 6625 0.0 39.966923 5 TATCAAC 6760 0.0 39.467342 2 AACGCAG 6740 0.0 39.45946 6 GTACATG 8565 0.0 33.526447 1 ACGCAGA 8045 0.0 33.05864 7 CGCAGAG 8150 0.0 32.558002 8 TACATGG 9130 0.0 30.983423 2 ACATGGG 8975 0.0 30.78861 3 GTGGTAT 1550 0.0 30.364298 1 TGGTATC 1560 0.0 28.631443 2 CATGGGA 5380 0.0 28.226984 4 GCAGAGT 9505 0.0 27.4159 9 GAGTACT 5135 0.0 25.779238 12-13 CAGAGTA 9020 0.0 25.064413 10-11 GTACTTT 5490 0.0 24.112272 14-15 AGAGTAC 8885 0.0 24.039062 10-11 >>END_MODULE