FastQCFastQC Report
Thu 26 May 2016
SRR1364447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994523
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.055.064481
GTATCAA32450.046.9659921
TCAACGC34750.041.079254
ATCAACG34950.040.843153
CAACGCA36200.039.2724575
GTACATG96750.038.704181
TATCAAC38900.037.766132
AACGCAG37700.037.3915146
TACATGG99100.036.550962
ACATGGG101400.034.3727953
CATGGGA63250.032.255194
ACGCAGA45400.031.0497847
CGCAGAG46000.030.2568768
CATGGGG46350.026.0502784
ATGGGAT27550.025.909465
GAGTACA70800.025.730391
TAAGGTG10800.024.2341425
ATGGGAG30700.023.6385145
AGTACAT74800.023.0595872
GTAAGGT9950.022.7157234
Produced by FastQC (version 0.11.5)