##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364447_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1994523 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.698360961493048 31.0 31.0 34.0 27.0 34.0 2 30.739935814227263 31.0 31.0 34.0 27.0 34.0 3 30.65095413790666 31.0 31.0 34.0 26.0 34.0 4 34.22983941523863 37.0 35.0 37.0 30.0 37.0 5 34.402646647845124 37.0 35.0 37.0 30.0 37.0 6 34.46578906334998 37.0 35.0 37.0 31.0 37.0 7 34.368273015653365 37.0 35.0 37.0 30.0 37.0 8 34.220083197837276 37.0 35.0 37.0 30.0 37.0 9 35.29850295032948 39.0 35.0 39.0 28.0 39.0 10-11 35.53835002153397 39.0 35.0 39.0 29.5 39.0 12-13 35.58789570238097 39.0 35.0 39.0 30.0 39.0 14-15 36.73783832023998 40.0 36.0 41.0 30.0 41.0 16-17 36.8812357641401 40.0 37.0 41.0 30.0 41.0 18-19 36.854096443109455 40.0 36.5 41.0 30.0 41.0 20-21 36.80400301224904 40.0 37.0 41.0 30.0 41.0 22-23 36.71598196661557 40.0 36.0 41.0 29.5 41.0 24-25 36.77324202328076 40.0 36.0 41.0 30.0 41.0 26-27 36.78283003003726 40.0 36.0 41.0 30.0 41.0 28-29 36.737022837039234 40.0 36.0 41.0 30.0 41.0 30-31 36.503219065410626 40.0 36.0 41.0 29.5 41.0 32-33 36.5814370152663 40.0 36.0 41.0 30.0 41.0 34-35 36.534272354843736 40.0 36.0 41.0 30.0 41.0 36-37 36.40972077032955 40.0 36.0 41.0 29.5 41.0 38-39 36.271999370275495 40.0 35.5 41.0 29.0 41.0 40-41 36.23353653981428 40.0 35.0 41.0 29.0 41.0 42-43 36.08944043262474 39.5 35.0 41.0 28.0 41.0 44-45 36.064989975046664 39.0 35.0 41.0 28.5 41.0 46-47 35.9182819651616 39.0 35.0 41.0 28.0 41.0 48-49 35.743506843490906 39.0 35.0 41.0 28.0 41.0 50-51 35.59930093561218 39.0 35.0 41.0 27.5 41.0 52-53 35.45900348103281 39.0 34.0 40.5 27.0 41.0 54-55 35.32527301013826 38.5 34.0 40.0 27.0 41.0 56-57 35.2699209284626 38.0 34.0 40.0 27.5 41.0 58-59 35.23205247570472 38.0 34.0 40.0 28.0 41.0 60-61 35.04099275866962 38.0 34.0 40.0 28.0 41.0 62-63 34.77456740283266 37.5 34.0 40.0 27.0 41.0 64-65 34.45410606947125 37.0 34.0 40.0 26.5 41.0 66-67 34.14116382714063 36.0 33.5 39.5 26.0 41.0 68-69 33.83225061831827 36.0 33.0 39.0 26.0 41.0 70-71 33.481632951838606 35.5 33.0 39.0 26.0 41.0 72-73 33.146342007587776 35.0 33.0 38.0 26.0 40.0 74-75 32.79733675670825 35.0 33.0 37.0 26.0 39.5 76-77 32.45868435711195 35.0 32.0 37.0 25.0 39.0 78-79 32.151274765946546 35.0 32.0 36.0 25.0 39.0 80-81 31.84306247659215 35.0 32.0 36.0 24.5 37.5 82-83 31.571984629909004 35.0 32.0 35.5 24.0 37.0 84-85 31.247791577234253 35.0 32.0 35.0 22.0 37.0 86-87 31.072950023639738 35.0 32.0 35.0 22.5 36.0 88-89 30.94226990613796 35.0 32.0 35.0 21.5 36.0 90-91 30.818547592582284 35.0 31.0 35.0 20.5 36.0 92-93 30.603672156199753 35.0 31.0 35.0 20.0 35.0 94-95 30.525510109434684 34.0 31.0 35.0 19.5 35.0 96-97 30.445714338716577 34.0 31.0 35.0 19.0 35.0 98-99 30.30204565201805 34.0 31.0 35.0 18.5 35.0 100-101 30.166437539201105 34.0 31.0 35.0 17.5 35.0 102-103 29.102244997926825 33.5 29.0 34.5 15.0 35.0 104-105 30.0811504805911 34.0 31.0 35.0 17.5 35.0 106-107 30.33522576575953 35.0 31.5 35.0 18.0 35.0 108-109 30.326963138554934 35.0 32.0 35.0 17.0 35.0 110-111 30.20865214389606 35.0 31.5 35.0 12.5 35.0 112-113 30.090181211247 35.0 31.0 35.0 7.5 35.0 114-115 29.983363190096078 35.0 31.0 35.0 2.0 35.0 116-117 29.855651200813426 35.0 31.0 35.0 2.0 35.0 118-119 29.592692839340536 35.0 31.0 35.0 2.0 35.0 120-121 29.45195166964733 34.5 31.0 35.0 2.0 35.0 122-123 29.214105578125697 34.0 31.0 35.0 2.0 35.0 124-125 28.015178566504375 33.5 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6940582892991216 1101 2 0.6995990065818631 1101 3 0.8050495101876614 1101 4 0.7292546152900243 1101 5 0.6203026944853534 1101 6 0.5741854044885457 1101 7 0.6621064259945371 1101 8 0.6777697733329191 1101 9 1.102056531686884 1101 10-11 0.9088339122315858 1101 12-13 0.8141259668197236 1101 14-15 0.9730729436801155 1101 16-17 0.6903007594182142 1101 18-19 0.7750734094328706 1101 20-21 0.8515560597489653 1101 22-23 0.7705290297919731 1101 24-25 0.8482690661242955 1101 26-27 0.8123112638100238 1101 28-29 0.8025648891345654 1101 30-31 1.032899569812436 1101 32-33 0.9334925026568541 1101 34-35 0.8489148194981908 1101 36-37 0.8331062271342233 1101 38-39 0.9644213560930766 1101 40-41 0.8479197658329269 1101 42-43 0.863153202164689 1101 44-45 0.6796103485369756 1101 46-47 0.6974218132763994 1101 48-49 0.7721390882934926 1101 50-51 0.6588833487181205 1101 52-53 0.5932245791266908 1101 54-55 0.568515911297844 1101 56-57 0.5641973690787054 1101 58-59 0.7311985653953172 1101 60-61 0.629401503867534 1101 62-63 0.7232503310615286 1101 64-65 0.6767349500149606 1101 66-67 0.6680945593740617 1101 68-69 0.6558248882941768 1101 70-71 0.5832984484921084 1101 72-73 0.6365055827767492 1101 74-75 0.5822466995678397 1101 76-77 0.6798782312213518 1101 78-79 0.6706164862473116 1101 80-81 0.662147904373203 1101 82-83 0.7558645543764619 1101 84-85 0.8186244743385949 1101 86-87 0.6849010573840957 1101 88-89 0.6132129211191355 1101 90-91 0.569099571176892 1101 92-93 0.7256591371942669 1101 94-95 0.6259440828147156 1101 96-97 0.4900053920362559 1101 98-99 0.6009250847663061 1101 100-101 0.5171240080373742 1101 102-103 0.49375988277371974 1101 104-105 0.42792974362583536 1101 106-107 0.41611991793215 1101 108-109 0.35480290781764623 1101 110-111 0.44736128790878027 1101 112-113 0.3317054582333583 1101 114-115 0.3335883487893234 1101 116-117 0.28156862715471576 1101 118-119 0.2788689501172392 1101 120-121 0.10116201679023362 1101 122-123 0.13286590686821498 1101 124-125 -0.13426297344376792 1102 1 0.16181982676346962 1102 2 0.14573157939320325 1102 3 0.20056469902181462 1102 4 0.10814580879101499 1102 5 0.08680083639184488 1102 6 0.020969930458640818 1102 7 0.16185201842388608 1102 8 0.18027441856668958 1102 9 0.5777716816307077 1102 10-11 0.27607232075288834 1102 12-13 0.3212191307640708 1102 14-15 0.3755916326439035 1102 16-17 0.21594407028158003 1102 18-19 0.21150797645387343 1102 20-21 0.3904769071260361 1102 22-23 0.26430603696508115 1102 24-25 0.1950568329551743 1102 26-27 0.20708186488166547 1102 28-29 0.23244886631890438 1102 30-31 0.30010800037160124 1102 32-33 0.22219405178829987 1102 34-35 0.1548596384421046 1102 36-37 0.09358852123153127 1102 38-39 0.0890240805766993 1102 40-41 0.11024691992506064 1102 42-43 0.19451906293731014 1102 44-45 -0.023565424948451152 1102 46-47 0.002094594367058278 1102 48-49 -0.05854106035398132 1102 50-51 -0.17452749520202104 1102 52-53 -0.16700924897805436 1102 54-55 -0.2307967561466313 1102 56-57 -0.1725477804330069 1102 58-59 -0.07376697345180361 1102 60-61 -0.16567562861885676 1102 62-63 -0.06652826413336044 1102 64-65 0.07222988346929071 1102 66-67 -0.07067099675764865 1102 68-69 -0.06916862998428286 1102 70-71 0.045758747947637346 1102 72-73 -0.005264251291180244 1102 74-75 0.09158929096011548 1102 76-77 0.10300096261881464 1102 78-79 -0.09970722172952406 1102 80-81 0.06925403176063938 1102 82-83 0.01580427436609 1102 84-85 0.08125761970200429 1102 86-87 -0.03849255595961054 1102 88-89 0.033548782323869375 1102 90-91 -0.013290671671612131 1102 92-93 -0.1246001316675418 1102 94-95 -0.003884529664759384 1102 96-97 -0.02899307395631112 1102 98-99 0.047825741580755476 1102 100-101 -0.10522685835274004 1102 102-103 -0.11295761139272997 1102 104-105 -0.048183492049563625 1102 106-107 -0.11848464303924189 1102 108-109 -0.12513562385636945 1102 110-111 -0.15514794854442115 1102 112-113 -0.12957975912037156 1102 114-115 -0.2676955722200951 1102 116-117 -0.4070062583348708 1102 118-119 -0.2844952794221278 1102 120-121 -0.34082900602542665 1102 122-123 -0.28808436752466093 1102 124-125 -0.5675214969075988 1103 1 0.7135940428888894 1103 2 0.6599043117560264 1103 3 0.9016285288090486 1103 4 0.7687281156219257 1103 5 0.6286611387434675 1103 6 0.5451510614776822 1103 7 0.6014936757587179 1103 8 0.7921795137514636 1103 9 1.250357067968892 1103 10-11 0.8891514780877188 1103 12-13 0.7508838844034855 1103 14-15 1.0575345217816476 1103 16-17 0.8554842438538088 1103 18-19 0.8552369798133341 1103 20-21 0.9632792587497718 1103 22-23 0.9118546791823192 1103 24-25 0.7288999180615505 1103 26-27 0.8829427271436998 1103 28-29 0.815387346162133 1103 30-31 1.0348346941006028 1103 32-33 0.930211269033542 1103 34-35 0.8681417633021198 1103 36-37 0.9155895010747628 1103 38-39 1.026277370050387 1103 40-41 0.9922055565431975 1103 42-43 1.0155489481556756 1103 44-45 0.840999244704399 1103 46-47 0.7779467080288711 1103 48-49 0.7781960617064883 1103 50-51 0.7804224628046939 1103 52-53 0.6779585561731309 1103 54-55 0.6720605366865087 1103 56-57 0.6768240700709143 1103 58-59 0.7660374078471861 1103 60-61 0.7369961938895457 1103 62-63 0.7447066882742561 1103 64-65 0.7249244523382714 1103 66-67 0.5513159853252247 1103 68-69 0.607169905849986 1103 70-71 0.656040102930838 1103 72-73 0.5282096428857557 1103 74-75 0.6086998032781423 1103 76-77 0.6133453075236233 1103 78-79 0.5942194999950061 1103 80-81 0.5729721868558251 1103 82-83 0.4979536304691159 1103 84-85 0.6116747529606776 1103 86-87 0.5774333958880966 1103 88-89 0.6093484043843418 1103 90-91 0.5654589783843775 1103 92-93 0.5074634881532845 1103 94-95 0.5325516405480037 1103 96-97 0.5822961197726926 1103 98-99 0.5387166990275638 1103 100-101 0.6259321562049287 1103 102-103 0.6122051125714343 1103 104-105 0.5138195874577178 1103 106-107 0.49545362683758043 1103 108-109 0.5246176656173169 1103 110-111 0.49139460464706985 1103 112-113 0.5325790454708965 1103 114-115 0.5117189714529147 1103 116-117 0.40704049418472366 1103 118-119 0.31854923345581554 1103 120-121 0.23632489934971446 1103 122-123 0.318172631355516 1103 124-125 0.10261078192778683 1104 1 0.4462292817249853 1104 2 0.502312260344592 1104 3 0.58214407978598 1104 4 0.38264480331289263 1104 5 0.4369291215315698 1104 6 0.3915124552488791 1104 7 0.4280510510845943 1104 8 0.5368954053221131 1104 9 1.069748659648873 1104 10-11 0.7154309383938298 1104 12-13 0.7158762280527426 1104 14-15 0.7403925306398804 1104 16-17 0.5841809297226703 1104 18-19 0.7277644340504281 1104 20-21 0.7580036476433349 1104 22-23 0.7468622917324339 1104 24-25 0.8076968197799346 1104 26-27 0.7836830454516672 1104 28-29 0.806558671464181 1104 30-31 0.7870380849704475 1104 32-33 0.8670481274418549 1104 34-35 0.7747152309807603 1104 36-37 0.6270650918577019 1104 38-39 0.8280004431729182 1104 40-41 0.7488928182677483 1104 42-43 0.6361222371277293 1104 44-45 0.5559022350612466 1104 46-47 0.568063927284328 1104 48-49 0.6003400851976437 1104 50-51 0.6477093902428095 1104 52-53 0.48195991924884396 1104 54-55 0.42746224981689096 1104 56-57 0.48291625638829316 1104 58-59 0.5727099525588741 1104 60-61 0.5965417973571547 1104 62-63 0.5882475402491352 1104 64-65 0.4777719315242024 1104 66-67 0.4116633593896353 1104 68-69 0.4811000753759558 1104 70-71 0.6159673413184166 1104 72-73 0.5573670676187419 1104 74-75 0.6191118708782923 1104 76-77 0.49671867463600705 1104 78-79 0.4618182879438706 1104 80-81 0.6142838493877072 1104 82-83 0.6234831801285203 1104 84-85 0.5323031869303492 1104 86-87 0.5353589453232424 1104 88-89 0.4664994272167391 1104 90-91 0.39276906759751284 1104 92-93 0.3381694404841795 1104 94-95 0.5014308524169273 1104 96-97 0.4346827286926036 1104 98-99 0.5461623687217347 1104 100-101 0.5062202066912924 1104 102-103 0.4687407365026992 1104 104-105 0.4917335560053573 1104 106-107 0.436160709224815 1104 108-109 0.2201772011904417 1104 110-111 0.29833937029615853 1104 112-113 0.40695160139554076 1104 114-115 0.37043761895943206 1104 116-117 0.3925100720166981 1104 118-119 0.2076801938460271 1104 120-121 0.09510968581286505 1104 122-123 0.2592691013748585 1104 124-125 0.18240677609704647 1105 1 0.1764651587930608 1105 2 0.2962289860763079 1105 3 0.37834135491842247 1105 4 0.264216721128399 1105 5 0.1464598789902638 1105 6 0.18541332387756881 1105 7 0.251681220872662 1105 8 0.34848343565707296 1105 9 0.8317041703646595 1105 10-11 0.4587377509884121 1105 12-13 0.5698183145069464 1105 14-15 0.18403638649137122 1105 16-17 0.3001370598232853 1105 18-19 0.4656676461248139 1105 20-21 0.7077131646721568 1105 22-23 0.7655787816296495 1105 24-25 0.720046736024635 1105 26-27 0.6242575408445958 1105 28-29 0.4862570033820717 1105 30-31 0.6877828366950425 1105 32-33 0.4666016808164457 1105 34-35 0.3901999581428015 1105 36-37 0.3925046954514073 1105 38-39 0.5476449992911014 1105 40-41 0.4596336642495231 1105 42-43 0.5280894969990371 1105 44-45 0.5447294160514602 1105 46-47 0.5930429618528166 1105 48-49 0.4434400637000948 1105 50-51 0.46912233261873837 1105 52-53 0.3113532945981774 1105 54-55 0.18858217634210206 1105 56-57 0.2816618424732127 1105 58-59 0.4326371375931757 1105 60-61 0.31335782942042556 1105 62-63 0.3685715891954402 1105 64-65 0.3731659752131833 1105 66-67 0.2315753045772695 1105 68-69 0.28774829884829956 1105 70-71 0.2861116622406428 1105 72-73 0.21301703856512688 1105 74-75 0.30340509106214597 1105 76-77 0.346782581953903 1105 78-79 0.20206744666602106 1105 80-81 0.12352875329792212 1105 82-83 0.12996229664310732 1105 84-85 0.25140391891125446 1105 86-87 0.29125512706054124 1105 88-89 0.362902685053232 1105 90-91 0.2736757852623235 1105 92-93 0.31657408821171984 1105 94-95 0.27708780474558026 1105 96-97 0.28356059920073307 1105 98-99 0.2924339806760763 1105 100-101 0.06541684753961263 1105 102-103 0.0687879353294889 1105 104-105 0.10041516027591868 1105 106-107 0.12580607416167666 1105 108-109 0.20007960018920912 1105 110-111 0.20916445048627352 1105 112-113 0.1651693055850103 1105 114-115 0.03537548891415909 1105 116-117 0.0543235605580783 1105 118-119 0.02031404801601866 1105 120-121 0.031219236045824772 1105 122-123 0.02766020138954417 1105 124-125 -0.2570225491140441 1106 1 0.5025844945805531 1106 2 0.4480439176158093 1106 3 0.566896666234932 1106 4 0.3306220814398486 1106 5 0.3077930710769152 1106 6 0.18853787696424718 1106 7 0.1246550814092231 1106 8 0.40379793021466526 1106 9 0.7626552019068029 1106 10-11 0.24804546570543806 1106 12-13 0.4013183542325791 1106 14-15 0.6185952995981765 1106 16-17 0.4839218528527667 1106 18-19 0.4497419065660324 1106 20-21 0.5750579036747823 1106 22-23 0.6573873570686217 1106 24-25 0.5496674886928758 1106 26-27 0.5983431892992712 1106 28-29 0.5157297118754158 1106 30-31 0.5541082160747877 1106 32-33 0.6422948930307442 1106 34-35 0.5903665409442596 1106 36-37 0.5287238749847631 1106 38-39 0.6023583126733385 1106 40-41 0.5964527007706124 1106 42-43 0.5659885409355851 1106 44-45 0.3576937424372204 1106 46-47 0.523747959336859 1106 48-49 0.4810793020273749 1106 50-51 0.4471026816745933 1106 52-53 0.37965436189339385 1106 54-55 0.3756996847506926 1106 56-57 0.3541007312149702 1106 58-59 0.4902461844429382 1106 60-61 0.3807242850998662 1106 62-63 0.44470178332134225 1106 64-65 0.44007770486704345 1106 66-67 0.42919203171517495 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70-71 -1.4297144584622359 2216 72-73 -1.482612212675086 2216 74-75 -1.3179123057924755 2216 76-77 -1.2929882684896867 2216 78-79 -1.3103716709111346 2216 80-81 -1.2126606011904535 2216 82-83 -1.5687080422728066 2216 84-85 -1.5663316310085733 2216 86-87 -1.5835573244917391 2216 88-89 -1.8963662345012438 2216 90-91 -1.7701281214412887 2216 92-93 -1.5569598951358046 2216 94-95 -1.4681167611979689 2216 96-97 -1.3831979238370664 2216 98-99 -1.5071013223546856 2216 100-101 -1.6698062100140163 2216 102-103 -1.8487261966127626 2216 104-105 -1.7421813766586602 2216 106-107 -1.5538042181642027 2216 108-109 -1.456784607815333 2216 110-111 -1.3882582061483895 2216 112-113 -1.547447257241842 2216 114-115 -1.422938515472083 2216 116-117 -1.4327160169026136 2216 118-119 -1.252569620302257 2216 120-121 -1.2311763099229722 2216 122-123 -1.164966269089561 2216 124-125 -1.1951105025922004 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31986.0 3 6503.0 4 4145.0 5 3681.0 6 3997.0 7 4561.0 8 5146.0 9 5656.0 10 6124.0 11 6688.0 12 7265.0 13 7877.0 14 7876.0 15 7594.0 16 7289.0 17 6488.0 18 6237.0 19 6180.0 20 6211.0 21 6773.0 22 7968.0 23 10456.0 24 14996.0 25 20885.0 26 24966.0 27 27634.0 28 33503.0 29 39372.0 30 47639.0 31 58175.0 32 73511.0 33 95904.0 34 131633.0 35 192236.0 36 343739.0 37 534819.0 38 188402.0 39 408.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.282270873050244 17.885493857982116 12.192905378449568 24.639329890518074 2 15.924749739034693 19.9287246342224 38.953621877566405 25.192903749176494 3 18.9915679044904 24.574359169765987 28.80905800988909 27.625014915854525 4 12.660988642112478 15.865945540501174 36.399189159241566 35.07387665814478 5 13.611071279231727 38.70721095175017 33.922037368876516 13.759680400141589 6 33.195806509836956 35.97732787838678 17.137356356416582 13.689509255359686 7 29.695872145871473 32.23768289460688 20.363916585569584 17.702528373952067 8 27.1128552469224 34.99154923080703 19.730277318596755 18.165318203673817 9 27.12288876822439 15.829443908807722 19.03489125906049 38.012776063907395 10-11 25.301489660525476 26.517902852412345 27.401299773810678 20.779307713251505 12-13 25.767179531918327 23.86450409906136 27.323549334388243 23.044767034632066 14-15 22.76055624775939 25.29919501011565 24.496336055073243 27.443912687051718 16-17 23.30662438962267 28.64249675225863 25.209316994098163 22.84156186402054 18-19 23.50687957983655 27.48068655646937 26.4037519465128 22.608681917181286 20-21 23.91416302986813 26.67153578955761 26.495249471090897 22.919051709483355 22-23 23.91803970403115 26.637472255740835 26.26627017624703 23.178217863980986 24-25 23.545828949014542 26.759700720570155 26.37187497414537 23.322595356269936 26-27 23.20801614179427 27.160930559998715 26.08384988211556 23.54720341609145 28-29 23.401498620114243 27.22822468070085 26.126873275142803 23.243403424042103 30-31 23.39782847603428 26.7875782390261 26.16422353713738 23.650369747802237 32-33 23.101276850063815 26.923729686732646 26.17448621281202 23.80050725039152 34-35 23.80231471140562 26.86006818238223 25.962125411161225 23.375491695050922 36-37 23.808142639331002 26.50982971783087 25.95578469531159 23.726242947526536 38-39 23.35486989196123 26.74747325854324 26.168109498271637 23.729547351223893 40-41 23.57358038661907 26.514800359288852 26.37376218697911 23.53785706711296 42-43 23.455505703664166 26.480905767390578 26.153649841172825 23.909938687772424 44-45 23.37183423004975 26.70257041994005 26.29028643283351 23.635308917176694 46-47 23.736378062507363 26.922112725841508 25.783422788338843 23.558086423312282 48-49 23.432925401467834 26.809030826291426 26.054155474739126 23.703888297501617 50-51 23.561159060010482 26.738964810184783 26.13595938481563 23.5639167449891 52-53 23.79001536571993 26.595205664733477 26.052303616998714 23.562475352547878 54-55 23.298310463463658 26.69174902168035 26.209138905721048 23.800801609134943 56-57 23.318819727172283 26.973144689045547 26.204943952509495 23.50309163127267 58-59 23.46774818293884 26.410621349550397 26.14923430342709 23.972396164083676 60-61 23.155457299171804 26.71472460160786 26.38210646287548 23.747711636344857 62-63 23.338375264601105 26.700297927743684 26.397556405822563 23.563770401832645 64-65 23.082359548026567 26.69418586868502 26.264826147671062 23.958628435617353 66-67 23.02098444205874 26.88359298335696 26.275054693732645 23.820367880851652 68-69 23.232241939402325 26.434443029567745 26.600467485957996 23.73284754507193 70-71 23.244403095924472 26.426868649339895 26.308087755464786 24.020640499270844 72-73 23.201617822523403 26.4959806406856 26.23831943821484 24.064082098576158 74-75 23.091678747055955 26.741418828559095 26.306775531407588 23.860126892977362 76-77 23.363754967624576 26.52538540142618 26.3046689403504 23.806190690598843 78-79 23.319885497848194 26.580910391386166 26.46751635727118 23.631687753494457 80-81 23.389241022840697 26.71308968020621 26.24093684429311 23.656732452659984 82-83 23.283414739695623 26.67615578773286 26.29725954618199 23.743169926389527 84-85 23.247420741873615 26.532148274435574 26.4244148876158 23.796016096075014 86-87 23.368515588312206 26.578669659962255 26.34589863930245 23.706916112423087 88-89 23.24353787615909 26.69320481628052 26.187472295154635 23.875785012405753 90-91 23.179746713576947 26.707344193097992 26.379610726251045 23.733298367074017 92-93 23.35803389016346 26.34130491845858 26.415614676327603 23.88504651505036 94-95 23.132319166096103 26.225738995882676 26.75517711407338 23.886764723947838 96-97 23.35401836281067 25.993315138663743 26.703794756906614 23.948871741618976 98-99 23.23078330870177 26.269377767916797 26.36030771274962 24.13953121063181 100-101 22.943394958674226 26.201646512511807 26.833410510212293 24.021548018601678 102-103 22.99353384895065 25.900456223297446 26.809944399274233 24.296065528477666 104-105 22.763342201015334 26.181172985986954 26.742993415551812 24.312491397445896 106-107 23.027419366565024 26.162780348990168 26.43461492274789 24.37518536169692 108-109 23.141075779910352 26.13364018330776 26.3631258586284 24.362158178153486 110-111 23.01813331862545 26.31549918647008 26.56529925115135 24.10106824375312 112-113 23.14484159814838 26.392327756601375 26.709321997354536 23.753508647895714 114-115 23.011198533404805 26.564412501961858 26.536783469113402 23.887605495519935 116-117 22.887065815178367 26.399555335373805 26.704572805057587 24.008806044390248 118-119 22.830790504539305 26.64719431285336 26.41585752387231 24.106157658735025 120-121 22.818864309965246 26.832971419687766 26.49255527750335 23.85560899284364 122-123 22.807907046021125 26.741420217159416 26.559250438436145 23.891422298383315 124-125 22.871908317882202 26.815345985213646 26.478138958944918 23.834606737959234 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 937.0 1 768.5 2 524.5 3 393.0 4 308.0 5 251.0 6 223.5 7 222.5 8 226.0 9 226.5 10 203.5 11 202.0 12 206.5 13 188.0 14 217.5 15 264.5 16 277.0 17 317.5 18 406.0 19 502.0 20 658.5 21 916.0 22 1341.0 23 2015.0 24 2772.5 25 3890.5 26 5575.0 27 7968.5 28 10690.5 29 13833.5 30 17770.0 31 22163.5 32 27952.5 33 31582.0 34 32923.5 35 35318.0 36 39384.5 37 44283.5 38 48143.0 39 51375.5 40 53911.5 41 57812.0 42 60930.5 43 63658.0 44 67832.5 45 72408.0 46 74319.0 47 75349.0 48 76969.5 49 76555.5 50 76869.5 51 76956.5 52 77120.5 53 78041.0 54 78917.5 55 75020.5 56 68366.5 57 63832.0 58 59682.0 59 53112.5 60 43466.0 61 35676.5 62 30224.0 63 23463.0 64 17088.0 65 13113.0 66 9793.0 67 7448.5 68 5666.5 69 4051.0 70 2850.0 71 1932.0 72 1462.0 73 1074.5 74 793.5 75 619.0 76 435.5 77 270.0 78 170.0 79 100.5 80 57.0 81 33.5 82 22.0 83 17.5 84 13.5 85 13.0 86 9.0 87 5.0 88 6.0 89 6.0 90 5.0 91 3.5 92 2.5 93 3.0 94 2.5 95 2.5 96 2.5 97 1.0 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0889435719718449 2 2.5068650499392587E-4 3 5.013730099878517E-5 4 0.0025068650499392585 5 0.0014539817289647699 6 1.5041190299635554E-4 7 0.0 8 0.0015041190299635552 9 6.016476119854222E-4 10-11 0.00882416497578619 12-13 0.0021057666419489773 14-15 0.004412082487893095 16-17 0.0030082380599271105 18-19 0.012609531201194471 20-21 0.016244485523606397 22-23 0.009601293141267361 24-25 0.010002391549257642 26-27 0.00987704829676068 28-29 0.007771281654811702 30-31 0.019729027943021966 32-33 0.0022311098944459403 34-35 9.776773694763108E-4 36-37 5.013730099878517E-4 38-39 3.760297574908888E-4 40-41 0.0013787757774665922 42-43 0.0029831694094277178 44-45 0.0012032952239708443 46-47 0.0019052174379538367 48-49 0.007721144353812916 50-51 0.004838249546382769 52-53 0.007445389198319598 54-55 0.007996899509306236 56-57 0.00940074393727222 58-59 0.00814731141230259 60-61 0.009726636393764324 62-63 0.01240898199719933 64-65 0.007921693557808057 66-67 0.011832403035713302 68-69 0.011957746288210265 70-71 0.004737974944385199 72-73 0.01190760898721148 74-75 0.012233501443703582 76-77 0.005464965808867584 78-79 0.007520595149817776 80-81 0.012709805803192042 82-83 0.01551749465912401 84-85 0.012434050647698725 86-87 0.014464611338149523 88-89 0.014389405386651346 90-91 0.011005137569233344 92-93 0.008448135218295301 94-95 0.007244839994324457 96-97 0.008322791965798338 98-99 0.006542917780341465 100-101 0.009350606636273436 102-103 0.01075445106423942 104-105 0.010152803452253997 106-107 0.005590309061364547 108-109 0.0031586499629234657 110-111 0.006417574527844502 112-113 0.008272654664799555 114-115 0.012233501443703582 116-117 0.010578970510743671 118-119 0.21814739664571428 120-121 0.024717689392401092 122-123 0.025269199703387726 124-125 0.2330131063918541 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1994523.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.88818811526326 #Duplication Level Percentage of deduplicated Percentage of total 1 84.60385980940227 61.66622049065074 2 9.293056090227442 13.547080409403817 3 2.866177558856343 6.267314672450016 4 1.2224081807467413 3.5639646972762082 5 0.6567762270395657 2.393561459304636 6 0.35211873209737293 1.5399177830412785 7 0.23386505090032578 1.1932199876525995 8 0.1520847363445631 0.8868144697714186 9 0.10406525471469126 0.6826615075716483 >10 0.48275246012082096 6.0933007638830325 >50 0.02563580371511218 1.2463296689997339 >100 0.00706163260421928 0.7830648110579076 >500 0.0 0.0 >1k 1.3846323052923E-4 0.13654927893694718 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.013730099878517E-5 2 0.0 0.0 0.0 0.0 5.013730099878517E-5 3 0.0 0.0 0.0 0.0 5.013730099878517E-5 4 0.0 0.0 0.0 0.0 5.013730099878517E-5 5 0.0 0.0 0.0 0.0 5.013730099878517E-5 6 0.0 0.0 0.0 0.0 5.013730099878517E-5 7 0.0 0.0 0.0 0.0 5.013730099878517E-5 8 0.0 0.0 0.0 0.0 5.013730099878517E-5 9 0.0 0.0 0.0 0.0 5.013730099878517E-5 10-11 0.0 0.0 0.0 0.0 5.013730099878517E-5 12-13 0.0 0.0 0.0 5.013730099878517E-5 4.2616705848967396E-4 14-15 0.0 0.0 0.0 5.013730099878517E-5 5.013730099878517E-4 16-17 0.0 0.0 0.0 5.013730099878517E-5 6.01647611985422E-4 18-19 0.0 0.0 0.0 7.520595149817776E-5 6.267162624848146E-4 20-21 0.0 0.0 0.0 1.504119029963555E-4 8.523341169793479E-4 22-23 0.0 0.0 0.0 2.5068650499392587E-4 9.024714179781331E-4 24-25 2.5068650499392587E-5 0.0 0.0 4.512357089890666E-4 9.024714179781331E-4 26-27 5.013730099878517E-5 0.0 0.0 0.001027814670475096 0.0010027460199757035 28-29 5.013730099878517E-5 0.0 0.0 0.0033090618659198214 0.0010027460199757035 30-31 5.013730099878517E-5 0.0 0.0 0.0131861101626805 0.0010027460199757035 32-33 5.013730099878517E-5 0.0 0.0 0.03454460038816298 0.0010027460199757035 34-35 5.013730099878517E-5 0.0 0.0 0.06507821669642316 0.0010528833209744886 36-37 5.013730099878517E-5 0.0 0.0 0.11358605541274781 0.0010528833209744886 38-39 5.013730099878517E-5 0.0 0.0 0.18941872317341038 0.0010528833209744886 40-41 5.013730099878517E-5 0.0 0.0 0.270490738888446 0.0010528833209744886 42-43 5.013730099878517E-5 0.0 0.0 0.34973274311702596 0.0010528833209744886 44-45 5.013730099878517E-5 0.0 0.0 0.4480018530746449 0.0010528833209744886 46-47 5.013730099878517E-5 0.0 0.0 0.5668272564417658 0.0010528833209744886 48-49 5.013730099878517E-5 0.0 0.0 0.6913933807732475 0.0011030206219732738 50-51 5.013730099878517E-5 0.0 0.0 0.833181667997812 0.001153157922972059 52-53 5.013730099878517E-5 0.0 0.0 0.9675997719755551 0.0013035698259684144 54-55 5.013730099878517E-5 0.0 0.0 1.1188389404383905 0.0013035698259684144 56-57 5.013730099878517E-5 0.0 0.0 1.290709608262226 0.0013537071269671998 58-59 5.013730099878517E-5 0.0 0.0 1.4807801163486207 0.0014289130784653774 60-61 5.013730099878517E-5 0.0 0.0 1.6927104876704857 0.0015041190299635552 62-63 5.013730099878517E-5 0.0 0.0 1.8956161448125692 0.0015542563309623404 64-65 5.013730099878517E-5 0.0 0.0 2.100176332887613 0.0015542563309623404 66-67 5.013730099878517E-5 0.0 0.0 2.3462000688886517 0.0016043936319611255 68-69 5.013730099878517E-5 0.0 0.0 2.620225487497512 0.0017046682339586958 70-71 5.013730099878517E-5 0.0 0.0 2.9183920165372874 0.0017046682339586958 72-73 5.013730099878517E-5 0.0 0.0 3.2295691751862474 0.0017798741854568737 74-75 5.013730099878517E-5 0.0 0.0 3.530367912528459 0.0018550801369550513 76-77 5.013730099878517E-5 0.0 0.0 3.858240792410015 0.0019052174379538367 78-79 5.013730099878517E-5 0.0 0.0 4.226022963886603 0.0019553547389526216 80-81 5.013730099878517E-5 0.0 0.0 4.635644713046679 0.0019804233894520143 82-83 5.013730099878517E-5 0.0 0.0 5.052360890298081 0.002055629340950192 84-85 5.013730099878517E-5 0.0 0.0 5.463536895789119 0.002055629340950192 86-87 5.013730099878517E-5 0.0 0.0 5.890004778084785 0.0020806979914495846 88-89 5.013730099878517E-5 0.0 0.0 6.3627243205518305 0.0021057666419489773 90-91 5.013730099878517E-5 5.013730099878517E-5 0.0 6.881043738277272 0.0021057666419489773 92-93 5.013730099878517E-5 5.013730099878517E-5 0.0 7.417437653012776 0.0021057666419489773 94-95 5.013730099878517E-5 5.013730099878517E-5 0.0 7.957015286361701 0.00213083529244837 96-97 5.013730099878517E-5 5.013730099878517E-5 0.0 8.499225128013062 0.0024567277489404736 98-99 5.013730099878517E-5 5.013730099878517E-5 0.0 9.060737830548959 0.002657276952935614 100-101 5.013730099878517E-5 5.013730099878517E-5 0.0 9.676398818163541 0.002657276952935614 102-103 5.013730099878517E-5 5.013730099878517E-5 0.0 10.33555391439457 0.0027074142539343996 104-105 5.013730099878517E-5 5.013730099878517E-5 0.0 10.994884491179095 0.0027074142539343996 106-107 5.013730099878517E-5 5.013730099878517E-5 0.0 11.644538568870853 0.0027074142539343996 108-109 5.013730099878517E-5 5.013730099878517E-5 0.0 12.317606766129044 0.0027575515549331845 110-111 5.013730099878517E-5 5.013730099878517E-5 0.0 13.036149495393133 0.0027575515549331845 112-113 5.013730099878517E-5 5.013730099878517E-5 0.0 13.791869033347822 0.0027575515549331845 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2065 0.0 55.06448 1 GTATCAA 3245 0.0 46.965992 1 TCAACGC 3475 0.0 41.07925 4 ATCAACG 3495 0.0 40.84315 3 CAACGCA 3620 0.0 39.272457 5 GTACATG 9675 0.0 38.70418 1 TATCAAC 3890 0.0 37.76613 2 AACGCAG 3770 0.0 37.391514 6 TACATGG 9910 0.0 36.55096 2 ACATGGG 10140 0.0 34.372795 3 CATGGGA 6325 0.0 32.25519 4 ACGCAGA 4540 0.0 31.049784 7 CGCAGAG 4600 0.0 30.256876 8 CATGGGG 4635 0.0 26.050278 4 ATGGGAT 2755 0.0 25.90946 5 GAGTACA 7080 0.0 25.73039 1 TAAGGTG 1080 0.0 24.234142 5 ATGGGAG 3070 0.0 23.638514 5 AGTACAT 7480 0.0 23.059587 2 GTAAGGT 995 0.0 22.715723 4 >>END_MODULE