FastQCFastQC Report
Thu 26 May 2016
SRR1364447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364447_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994523
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31550.15818318465116724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27620.13847922535864465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31400.068.503091
GTATCAA54250.052.6100121
ATCAACG67700.041.876043
TCAACGC68050.041.573144
CAACGCA69600.040.6940655
TATCAAC70000.040.3309562
AACGCAG72600.039.1744426
GTACATG97700.036.8362121
TACATGG100700.035.7836072
ACATGGG102850.034.2239763
ACGCAGA85550.033.3140227
CGCAGAG87650.032.2418988
CATGGGA64700.030.930174
ATGGGAT29350.026.5580735
GTGGTAT14550.026.2089481
GCAGAGT105950.026.1114449
CATGGGG45500.025.525284
GAGTACA75200.024.879661
GAGTACT54600.024.62604712-13
CAGAGTA98600.024.43751510-11
Produced by FastQC (version 0.11.5)