FastQCFastQC Report
Thu 26 May 2016
SRR1364446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2198488
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27000.049.1684151
TCAACGC27050.047.0539784
ATCAACG27400.046.2348063
CAACGCA28550.044.3754845
GGTATCA15700.044.3640671
AACGCAG29500.042.7448166
TATCAAC31500.040.216942
GTACATG74650.035.806481
TACATGG75300.034.91142
ACGCAGA37250.034.170957
ACATGGG76300.033.5963943
CGCAGAG38550.032.7100378
CATGGGG50550.028.238394
CATGGGA36650.024.1803234
GAGTACA54500.023.0478861
TAAGGTG10300.022.5214825
GCAGAGT56150.021.1870069
GTATATA12450.020.5611
GTGTAGC13650.020.4979461
ATGGGGA30350.020.381885
Produced by FastQC (version 0.11.5)