##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364446_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2198488 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.649857538453702 31.0 31.0 34.0 27.0 34.0 2 30.71983335819891 31.0 31.0 34.0 27.0 34.0 3 30.647166143276653 31.0 31.0 34.0 26.0 34.0 4 34.25917721634141 37.0 35.0 37.0 30.0 37.0 5 34.39595121738213 37.0 35.0 37.0 30.0 37.0 6 34.51254771461113 37.0 35.0 37.0 31.0 37.0 7 34.39310016702388 37.0 35.0 37.0 30.0 37.0 8 34.240447525754064 37.0 35.0 37.0 30.0 37.0 9 35.353232767247306 39.0 35.0 39.0 28.0 39.0 10-11 35.563646469755575 39.0 35.0 39.0 29.5 39.0 12-13 35.60553321191655 39.0 35.0 39.0 30.0 39.0 14-15 36.746812809530915 40.0 36.0 41.0 30.0 41.0 16-17 36.88169346387153 40.0 36.0 41.0 30.0 41.0 18-19 36.85395462699819 40.0 36.5 41.0 30.0 41.0 20-21 36.79806462441459 40.0 36.5 41.0 30.0 41.0 22-23 36.70291059127909 40.0 36.0 41.0 29.5 41.0 24-25 36.7426260684616 40.0 36.0 41.0 30.0 41.0 26-27 36.74052030304463 40.0 36.0 41.0 30.0 41.0 28-29 36.68729417672509 40.0 36.0 41.0 30.0 41.0 30-31 36.45882488328342 40.0 36.0 41.0 29.0 41.0 32-33 36.52604926658685 40.0 36.0 41.0 30.0 41.0 34-35 36.47291161016116 40.0 36.0 41.0 30.0 41.0 36-37 36.35639516795179 40.0 36.0 41.0 29.0 41.0 38-39 36.209303393968945 40.0 35.0 41.0 28.5 41.0 40-41 36.17404666297928 40.0 35.0 41.0 29.0 41.0 42-43 36.036222622093 39.5 35.0 41.0 28.0 41.0 44-45 36.014062846829276 39.0 35.0 41.0 28.0 41.0 46-47 35.86440476363755 39.0 35.0 41.0 28.0 41.0 48-49 35.69118594233855 39.0 35.0 41.0 27.5 41.0 50-51 35.55538988613993 39.0 34.5 41.0 27.5 41.0 52-53 35.421245647008305 39.0 34.0 40.5 27.0 41.0 54-55 35.293450771621224 38.5 34.0 40.0 27.0 41.0 56-57 35.24411208976351 38.5 34.0 40.0 27.5 41.0 58-59 35.21402891441754 38.0 34.0 40.0 28.0 41.0 60-61 35.01792027065874 38.0 34.0 40.0 27.0 41.0 62-63 34.757382346412626 37.5 34.0 40.0 27.0 41.0 64-65 34.44999904479806 37.0 34.0 40.0 26.0 41.0 66-67 34.135175402367445 36.5 33.5 39.5 26.0 41.0 68-69 33.829324290148506 36.0 33.0 39.0 26.0 41.0 70-71 33.475443350157015 35.5 33.0 39.0 26.0 41.0 72-73 33.14771970554308 35.0 33.0 38.5 26.0 40.0 74-75 32.79444418163756 35.0 33.0 37.0 25.5 39.5 76-77 32.45083302706223 35.0 32.0 37.0 25.0 39.0 78-79 32.139473583662955 35.0 32.0 36.0 25.0 39.0 80-81 31.83283602184774 35.0 32.0 36.0 24.0 37.5 82-83 31.55252837404616 35.0 32.0 35.5 24.0 37.0 84-85 31.22287021807715 35.0 31.5 35.0 21.5 37.0 86-87 31.04078666792814 35.0 31.0 35.0 21.5 36.0 88-89 30.905145036042953 35.0 31.0 35.0 20.5 36.0 90-91 30.781602401286705 35.0 31.0 35.0 20.0 36.0 92-93 30.56284819384959 34.0 31.0 35.0 19.5 35.5 94-95 30.485844362125242 34.0 31.0 35.0 19.5 35.0 96-97 30.41363518927554 34.0 31.0 35.0 19.0 35.0 98-99 30.26037667706169 34.0 31.0 35.0 18.0 35.0 100-101 30.13184015559785 34.0 31.0 35.0 17.0 35.0 102-103 29.087005250881514 33.5 29.0 34.5 14.0 35.0 104-105 30.050637074207366 34.0 30.5 35.0 17.0 35.0 106-107 30.300995274934408 35.0 31.0 35.0 17.5 35.0 108-109 30.294519915505568 35.0 32.0 35.0 16.0 35.0 110-111 30.179687357856857 35.0 31.0 35.0 10.5 35.0 112-113 30.064415862174368 35.0 31.0 35.0 7.0 35.0 114-115 29.962201294708 35.0 31.0 35.0 2.0 35.0 116-117 29.834559479060154 35.0 31.0 35.0 2.0 35.0 118-119 29.570951262867936 34.5 31.0 35.0 2.0 35.0 120-121 29.43142878196288 34.0 31.0 35.0 2.0 35.0 122-123 29.204208756199716 34.0 31.0 35.0 2.0 35.0 124-125 28.026562573914436 33.5 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7410190920731736 1101 2 0.699263346732149 1101 3 0.9359338669047581 1101 4 0.785689786139578 1101 5 0.6887362574878679 1101 6 0.7665397876069875 1101 7 0.86869611038437 1101 8 0.9636744665619688 1101 9 1.3600177036877525 1101 10-11 1.1271464010648273 1101 12-13 1.0216226159223538 1101 14-15 1.2051328329544475 1101 16-17 0.9694315478414381 1101 18-19 1.0860306740779038 1101 20-21 1.1439770500010553 1101 22-23 1.128128942435218 1101 24-25 1.1013980559664631 1101 26-27 1.0578275513965636 1101 28-29 1.0074037453440141 1101 30-31 1.0839872546221656 1101 32-33 0.9793549091969425 1101 34-35 0.9998097249237787 1101 36-37 0.9660995976425255 1101 38-39 1.0483919247324422 1101 40-41 1.0677562409321766 1101 42-43 1.055026186674752 1101 44-45 0.9305409024941085 1101 46-47 0.9963346369534989 1101 48-49 0.9512877428432702 1101 50-51 0.8489342836674538 1101 52-53 0.7606589654960487 1101 54-55 0.7235253595417959 1101 56-57 0.7452526342146157 1101 58-59 0.8464394157002388 1101 60-61 0.8197133728837827 1101 62-63 0.896224870349208 1101 64-65 0.7784465409620864 1101 66-67 0.8109645172500919 1101 68-69 0.7449969568498744 1101 70-71 0.6909703850392575 1101 72-73 0.7182839289197247 1101 74-75 0.7682985642251339 1101 76-77 0.8427667721638556 1101 78-79 0.6425714138179615 1101 80-81 0.7145656580397883 1101 82-83 0.7672318396524176 1101 84-85 0.8972145042279713 1101 86-87 0.8169330122670253 1101 88-89 0.7286640499089501 1101 90-91 0.6562338795003768 1101 92-93 0.8290732361746151 1101 94-95 0.716703084699823 1101 96-97 0.7329662007699582 1101 98-99 0.7873965840697643 1101 100-101 0.7409584850345325 1101 102-103 0.7441741666476602 1101 104-105 0.6997753506043658 1101 106-107 0.6022609257093059 1101 108-109 0.6402580353303442 1101 110-111 0.7367877489846784 1101 112-113 0.5755195655277063 1101 114-115 0.617499311874365 1101 116-117 0.5757949219521201 1101 118-119 0.5749900316508452 1101 120-121 0.4250522062582647 1101 122-123 0.4045507921298892 1101 124-125 0.14216156014461134 1102 1 0.24467568594617006 1102 2 0.2963342254728758 1102 3 0.3517054237445336 1102 4 0.2876008146926239 1102 5 0.2560941511979067 1102 6 0.14714768812611823 1102 7 0.23334400958432866 1102 8 0.34457178633605423 1102 9 0.7623695527469962 1102 10-11 0.43378971289127577 1102 12-13 0.3410267846908468 1102 14-15 0.5439575471311713 1102 16-17 0.3461755359743748 1102 18-19 0.41266706107443696 1102 20-21 0.5467772987658677 1102 22-23 0.5476290128756531 1102 24-25 0.4657008448942932 1102 26-27 0.3511409136990835 1102 28-29 0.4355604623028597 1102 30-31 0.519294654679193 1102 32-33 0.40356835407010294 1102 34-35 0.2652843659755959 1102 36-37 0.3604902417604805 1102 38-39 0.43630887967989196 1102 40-41 0.37617792356839175 1102 42-43 0.470224430597753 1102 44-45 0.27822483926427566 1102 46-47 0.38931007660579553 1102 48-49 0.46523629774403474 1102 50-51 0.31951676558809794 1102 52-53 0.2532207775216335 1102 54-55 0.18360660403414641 1102 56-57 0.19864025352718073 1102 58-59 0.27166495779355415 1102 60-61 0.23952892423486105 1102 62-63 0.28775630283789866 1102 64-65 0.3159540059513688 1102 66-67 0.16659534462978343 1102 68-69 0.2101305513334637 1102 70-71 0.3182348883941586 1102 72-73 0.29764419402288667 1102 74-75 0.4060130083604747 1102 76-77 0.40950127455025864 1102 78-79 0.37644144612483643 1102 80-81 0.4365661942398589 1102 82-83 0.2942261893636342 1102 84-85 0.48900113210119756 1102 86-87 0.38068530419167956 1102 88-89 0.4388044008441554 1102 90-91 0.3782822316597354 1102 92-93 0.39048324223188047 1102 94-95 0.3628011153349284 1102 96-97 0.2470315327723931 1102 98-99 0.2458231527225614 1102 100-101 0.19005237043059964 1102 102-103 0.15867514163203822 1102 104-105 0.18910342891011211 1102 106-107 0.21597750141832606 1102 108-109 0.3072116992728162 1102 110-111 0.294910592752494 1102 112-113 0.20113339583491552 1102 114-115 0.10434222204675692 1102 116-117 -0.03049912888107542 1102 118-119 0.13851903740346572 1102 120-121 0.01457760479928183 1102 122-123 0.034567998043684156 1102 124-125 -0.4140990282188035 1103 1 0.47325661630853944 1103 2 0.5200181724770481 1103 3 0.669418568359788 1103 4 0.5620976578398498 1103 5 0.5188223668594318 1103 6 0.4036016519183008 1103 7 0.48480652468137464 1103 8 0.55976949695701 1103 9 0.9715223413611653 1103 10-11 0.696889944911554 1103 12-13 0.5596021683305636 1103 14-15 0.7313213341751919 1103 16-17 0.5705622569501543 1103 18-19 0.6969859354808321 1103 20-21 0.6932917899978221 1103 22-23 0.612067223047184 1103 24-25 0.5051933296567057 1103 26-27 0.6153164517840466 1103 28-29 0.5381757338666375 1103 30-31 0.8280535340125752 1103 32-33 0.7082841771515689 1103 34-35 0.6317951748644859 1103 36-37 0.6855021874567484 1103 38-39 0.6873775026872906 1103 40-41 0.6279364701671781 1103 42-43 0.7309812947852237 1103 44-45 0.5967594627012787 1103 46-47 0.5672124596164139 1103 48-49 0.5429420742349222 1103 50-51 0.5313713144121053 1103 52-53 0.4625655052599029 1103 54-55 0.44900512011177796 1103 56-57 0.4905347064349215 1103 58-59 0.5805354936079894 1103 60-61 0.49081965682429995 1103 62-63 0.515275937918048 1103 64-65 0.5125581189154005 1103 66-67 0.43124260770938605 1103 68-69 0.48703179087512183 1103 70-71 0.5448224196401341 1103 72-73 0.40111008339770393 1103 74-75 0.5925776220922216 1103 76-77 0.527676508523875 1103 78-79 0.4501867698113031 1103 80-81 0.47984868161275074 1103 82-83 0.37768012573594234 1103 84-85 0.5814991574834103 1103 86-87 0.5706150843848512 1103 88-89 0.5204722203202934 1103 90-91 0.46071651829645077 1103 92-93 0.3722941951739642 1103 94-95 0.4261538766667492 1103 96-97 0.5322146919494095 1103 98-99 0.5315417300831804 1103 100-101 0.4617951493119996 1103 102-103 0.3867935872397297 1103 104-105 0.40387243537458417 1103 106-107 0.3993534587609098 1103 108-109 0.42377491966233904 1103 110-111 0.42662380974246616 1103 112-113 0.47080296571334657 1103 114-115 0.5426072763101715 1103 116-117 0.4521321424805329 1103 118-119 0.39172082352895643 1103 120-121 0.2905069430081362 1103 122-123 0.32399496305806963 1103 124-125 0.1532300017700301 1104 1 0.37796023257633493 1104 2 0.46438605386281395 1104 3 0.6162036827217285 1104 4 0.4128653677821603 1104 5 0.4323513858461112 1104 6 0.3773370104631226 1104 7 0.4247125986475879 1104 8 0.46422758292426636 1104 9 0.8888874504788475 1104 10-11 0.6313489248828716 1104 12-13 0.5797642696593002 1104 14-15 0.6829924159671492 1104 16-17 0.6476897834205886 1104 18-19 0.637660425182041 1104 20-21 0.7640784322939709 1104 22-23 0.7293197903145767 1104 24-25 0.7706693739839139 1104 26-27 0.7753350504904333 1104 28-29 0.6965917126706174 1104 30-31 0.6892546562657103 1104 32-33 0.6228346706289756 1104 34-35 0.649150103457508 1104 36-37 0.49425431606329795 1104 38-39 0.58969100552779 1104 40-41 0.6159278997053335 1104 42-43 0.43372118195250664 1104 44-45 0.3802656867232983 1104 46-47 0.4048121314442028 1104 48-49 0.28766199645229307 1104 50-51 0.4087225985484153 1104 52-53 0.19709219855277382 1104 54-55 0.21238179481877495 1104 56-57 0.22198286040210036 1104 58-59 0.279576684951806 1104 60-61 0.3773561696181815 1104 62-63 0.3622298008924574 1104 64-65 0.2242682887890055 1104 66-67 0.2739724084576949 1104 68-69 0.3930371027135777 1104 70-71 0.4317945990038723 1104 72-73 0.3789205605263888 1104 74-75 0.345765730383917 1104 76-77 0.390015069143125 1104 78-79 0.2791958753923325 1104 80-81 0.4292776525627531 1104 82-83 0.3771298641007377 1104 84-85 0.3750814488457479 1104 86-87 0.42379489426568995 1104 88-89 0.4604673097142822 1104 90-91 0.46295242671792636 1104 92-93 0.40976712374731505 1104 94-95 0.4221253831748015 1104 96-97 0.36613209367419586 1104 98-99 0.3664585770121498 1104 100-101 0.3562607936771869 1104 102-103 0.32508985505083743 1104 104-105 0.35552093180092115 1104 106-107 0.23883195069156216 1104 108-109 0.1488779119238579 1104 110-111 0.31298780969626705 1104 112-113 0.351094539361533 1104 114-115 0.34553858300617435 1104 116-117 0.35920628108195984 1104 118-119 0.3250634961391121 1104 120-121 0.2528342109445738 1104 122-123 0.3104146136949737 1104 124-125 0.1476692965419879 1105 1 0.45608649453421535 1105 2 0.5334396232169354 1105 3 0.5570953095553861 1105 4 0.48877820082734047 1105 5 0.4123035479350108 1105 6 0.41185451861944244 1105 7 0.570296868391793 1105 8 0.632042481442106 1105 9 1.014704816210788 1105 10-11 0.6289737100441855 1105 12-13 0.6666936519323841 1105 14-15 0.4215925719658884 1105 16-17 0.5052977431860199 1105 18-19 0.5609425348160428 1105 20-21 0.7429536669197319 1105 22-23 0.8185567709517514 1105 24-25 0.7898518997422102 1105 26-27 0.8560420575401579 1105 28-29 0.8362302432527073 1105 30-31 0.8897288651976325 1105 32-33 0.8058102976345438 1105 34-35 0.7855347029011739 1105 36-37 0.881917947719046 1105 38-39 0.903172683761035 1105 40-41 0.8480429166477492 1105 42-43 0.8623954182033629 1105 44-45 0.8246014559234638 1105 46-47 0.8928064838302845 1105 48-49 0.7364926229280826 1105 50-51 0.7991471440520925 1105 52-53 0.6721272514608572 1105 54-55 0.6034225055534606 1105 56-57 0.6149460236786481 1105 58-59 0.7087594702824802 1105 60-61 0.6259910529957153 1105 62-63 0.7019551818361904 1105 64-65 0.7165718403773127 1105 66-67 0.6676192683341498 1105 68-69 0.6298675283399291 1105 70-71 0.6433014846928771 1105 72-73 0.6270924689237276 1105 74-75 0.659367535305492 1105 76-77 0.6331222525318552 1105 78-79 0.6511237193799744 1105 80-81 0.6319386097720354 1105 82-83 0.5509539055233788 1105 84-85 0.7010045158507374 1105 86-87 0.6893800235774137 1105 88-89 0.6498658209603612 1105 90-91 0.7028693380280018 1105 92-93 0.676788496766001 1105 94-95 0.7471604573593105 1105 96-97 0.7173689560144574 1105 98-99 0.7161297073306692 1105 100-101 0.5638381792217295 1105 102-103 0.5861020426118735 1105 104-105 0.4758335760816692 1105 106-107 0.44288898171982183 1105 108-109 0.5837296301440098 1105 110-111 0.6265617850556175 1105 112-113 0.554927146672366 1105 114-115 0.4227771925663575 1105 116-117 0.37590597585038665 1105 118-119 0.17568517196386324 1105 120-121 0.19558122703253034 1105 122-123 0.296033964006611 1105 124-125 0.08336709302788847 1106 1 0.35559726677145775 1106 2 0.40118510337952884 1106 3 0.5777208669149942 1106 4 0.31660294528738575 1106 5 0.3128640032642096 1106 6 0.25995232463002793 1106 7 0.18930647743621876 1106 8 0.45372400004781355 1106 9 0.8110672502338048 1106 10-11 0.3704168200821911 1106 12-13 0.5020384370582178 1106 14-15 0.6616402668600898 1106 16-17 0.5173448619468459 1106 18-19 0.4736370838867572 1106 20-21 0.6189344984317628 1106 22-23 0.6144715829904115 1106 24-25 0.45959896643945086 1106 26-27 0.4978895362022442 1106 28-29 0.3964707364450675 1106 30-31 0.5426914949186923 1106 32-33 0.5562782542479923 1106 34-35 0.5613708680520091 1106 36-37 0.5600313485257686 1106 38-39 0.641780088583694 1106 40-41 0.6275527558333422 1106 42-43 0.6485734115041168 1106 44-45 0.28389621109800345 1106 46-47 0.42452568696950976 1106 48-49 0.4141904428824148 1106 50-51 0.4409316677431079 1106 52-53 0.40492502240396533 1106 54-55 0.29868363514253815 1106 56-57 0.21313674463620202 1106 58-59 0.30960323165296444 1106 60-61 0.31064041114984775 1106 62-63 0.3722145164281656 1106 64-65 0.48027594958527686 1106 66-67 0.31471169386258424 1106 68-69 0.41167084487023686 1106 70-71 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1.0751807591072478 2203 54-55 1.0343020760672914 2203 56-57 1.0488644048759213 2203 58-59 0.9133228557032993 2203 60-61 0.7986289757513134 2203 62-63 0.8368161276819848 2203 64-65 0.9072764989089563 2203 66-67 0.926024498554348 2203 68-69 0.8760505035437376 2203 70-71 0.8908511566304256 2203 72-73 0.9083552305995681 2203 74-75 0.8607196386367946 2203 76-77 0.9475617899339497 2203 78-79 0.9084775514814538 2203 80-81 0.9473764273791829 2203 82-83 0.9796223727621864 2203 84-85 1.0491205516094873 2203 86-87 1.0344605280690047 2203 88-89 1.0402191849683184 2203 90-91 0.9948888871462387 2203 92-93 1.0973505490178042 2203 94-95 1.0600255297463796 2203 96-97 1.0760668050970992 2203 98-99 1.1715572620908823 2203 100-101 1.2420497288425771 2203 102-103 1.2081857353864933 2203 104-105 1.1370553761221984 2203 106-107 1.157214498995959 2203 108-109 1.2181910050064886 2203 110-111 1.1998531573946707 2203 112-113 1.2627996788684683 2203 114-115 1.198330965155062 2203 116-117 1.3876787782358662 2203 118-119 1.415440491263361 2203 120-121 1.4186282224485964 2203 122-123 1.494161840184134 2203 124-125 1.554075864637948 2204 1 0.6784126534575634 2204 2 0.7044670123842209 2204 3 0.7838247601897379 2204 4 0.7739294922379898 2204 5 0.5649842940247183 2204 6 0.6213583257861615 2204 7 0.718336041303246 2204 8 0.7528306809573806 2204 9 1.2180609615225961 2204 10-11 0.9920353638055772 2204 12-13 0.8755672069589195 2204 14-15 0.9605597374350907 2204 16-17 0.9027764162924967 2204 18-19 0.9831816016290489 2204 20-21 0.9952635459289212 2204 22-23 1.0783083814816834 2204 24-25 1.0590281742859062 2204 26-27 1.064549155331811 2204 28-29 0.9888976942664343 2204 30-31 1.1997029060936555 2204 32-33 1.1089641049978738 2204 34-35 1.0728997180884576 2204 36-37 1.0829099101902244 2204 38-39 1.1379236529439822 2204 40-41 0.9846376507115835 2204 42-43 1.1054633936797487 2204 44-45 1.103684528239505 2204 46-47 1.1078892138449419 2204 48-49 1.0400777712421458 2204 50-51 0.9339212485397752 2204 52-53 0.963840552322992 2204 54-55 0.9042767797237659 2204 56-57 0.8913815033303436 2204 58-59 0.8697070852488409 2204 60-61 0.8323050718294098 2204 62-63 0.6925806084255584 2204 64-65 0.7856253414483092 2204 66-67 0.7602091734765892 2204 68-69 0.762432309533871 2204 70-71 0.7519411680977939 2204 72-73 0.9146454409068809 2204 74-75 0.8239706407730836 2204 76-77 0.7995614360793653 2204 78-79 0.8452941843499744 2204 80-81 0.7508518290660788 2204 82-83 0.784065648015364 2204 84-85 0.9816472784701524 2204 86-87 0.9148088038216002 2204 88-89 0.8749808093234286 2204 90-91 0.7963819869820092 2204 92-93 0.9432750463623449 2204 94-95 1.0110831639072053 2204 96-97 0.8890439183716055 2204 98-99 1.0127083037938398 2204 100-101 1.0780275831210808 2204 102-103 1.1049842806338077 2204 104-105 1.0410595464958163 2204 106-107 0.9822278943661154 2204 108-109 1.0515137885179477 2204 110-111 1.0899012684539073 2204 112-113 1.133699863229026 2204 114-115 1.0949457112121372 2204 116-117 1.1583555580198563 2204 118-119 1.1593935075832604 2204 120-121 1.1798464578060255 2204 122-123 1.3172704603758376 2204 124-125 1.2947653080796755 2205 1 0.3958650109974151 2205 2 0.5067427965679485 2205 3 0.5761238027363653 2205 4 0.5204849787255768 2205 5 0.49975468023190217 2205 6 0.46250820429639816 2205 7 0.5049567687933774 2205 8 0.5255651125817451 2205 9 1.0338397175866163 2205 10-11 0.7120700479098119 2205 12-13 0.7322537283414476 2205 14-15 0.8197316349783748 2205 16-17 0.6236041274433717 2205 18-19 0.621745921894771 2205 20-21 0.7555511907680525 2205 22-23 0.8872203039867657 2205 24-25 0.778061767600768 2205 26-27 0.7427946625663964 2205 28-29 0.6983072565743029 2205 30-31 0.9333696346035154 2205 32-33 0.8064397637301184 2205 34-35 0.8131551804552544 2205 36-37 0.7065691766338773 2205 38-39 0.9216345055682709 2205 40-41 0.7726265859060746 2205 42-43 0.6848051315877655 2205 44-45 0.606806360438604 2205 46-47 0.6891472309911393 2205 48-49 0.646207265832679 2205 50-51 0.6357399154639367 2205 52-53 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0.8615390007848518 2208 116-117 0.8566342047851307 2208 118-119 0.8832569487218649 2208 120-121 0.9365996770731293 2208 122-123 0.970314936420575 2208 124-125 1.000417138896239 2209 1 0.30550431292325086 2209 2 0.1701093397407405 2209 3 0.41561262083878603 2209 4 0.38221946967492215 2209 5 0.24392844292896854 2209 6 0.16637044743470142 2209 7 0.21552390885322836 2209 8 0.3850777915644201 2209 9 0.8761151443914628 2209 10-11 0.511081766616357 2209 12-13 0.4411345616191511 2209 14-15 0.6640530375239067 2209 16-17 0.5948526011536472 2209 18-19 0.4429430354340411 2209 20-21 0.65753170814898 2209 22-23 0.6668364525493402 2209 24-25 0.6524837592454631 2209 26-27 0.6241572307800709 2209 28-29 0.6095872330437047 2209 30-31 0.8144533246505787 2209 32-33 0.5457486952021497 2209 34-35 0.5946890564384759 2209 36-37 0.6891566097413602 2209 38-39 0.6883865784018397 2209 40-41 0.6072116332732165 2209 42-43 0.6959008494237295 2209 44-45 0.6085472520699469 2209 46-47 0.6226627542964422 2209 48-49 0.5644010479577801 2209 50-51 0.6256282894473415 2209 52-53 0.5537967238623196 2209 54-55 0.5245854542034962 2209 56-57 0.5671589090575253 2209 58-59 0.5901915086304186 2209 60-61 0.4753436188217961 2209 62-63 0.5221039861049164 2209 64-65 0.5746432103784898 2209 66-67 0.4764183904300978 2209 68-69 0.5061468297086122 2209 70-71 0.5419348259852725 2209 72-73 0.4307706651873815 2209 74-75 0.44839324974790884 2209 76-77 0.49424544435449747 2209 78-79 0.44136565632016556 2209 80-81 0.4593285256468107 2209 82-83 0.5090475893093753 2209 84-85 0.5912632487421092 2209 86-87 0.542179688761955 2209 88-89 0.4857790341320012 2209 90-91 0.4986084585321393 2209 92-93 0.4779415735100372 2209 94-95 0.5066587809411267 2209 96-97 0.4580625861604233 2209 98-99 0.5044659015771416 2209 100-101 0.6216093539919214 2209 102-103 0.6253237655537554 2209 104-105 0.5766275087386816 2209 106-107 0.5764013269529471 2209 108-109 0.4875262424616871 2209 110-111 0.5830716833803358 2209 112-113 0.6675702508517389 2209 114-115 0.7121591834943679 2209 116-117 0.7652969457985925 2209 118-119 0.8017952179658749 2209 120-121 0.7554151101427422 2209 122-123 0.7343192102558191 2209 124-125 0.7962446967765402 2210 1 0.07197369645493978 2210 2 0.10371053300597666 2210 3 0.3046668885060875 2210 4 0.1998622371964487 2210 5 0.27942858805230486 2210 6 0.1100677995520698 2210 7 0.17103644803002283 2210 8 0.26594503690225224 2210 9 0.4459517634993304 2210 10-11 0.21192099273803677 2210 12-13 0.13346861666450138 2210 14-15 0.3098094164428886 2210 16-17 0.3078193874331703 2210 18-19 0.3087301033829988 2210 20-21 0.3496703266399095 2210 22-23 0.3442897120975488 2210 24-25 0.2384294753087275 2210 26-27 0.304696557576996 2210 28-29 0.32814541643795536 2210 30-31 0.6215319431024611 2210 32-33 0.3220710909215896 2210 34-35 0.4370390717968178 2210 36-37 0.34402566033539017 2210 38-39 0.42329497173684416 2210 40-41 0.42265859687653773 2210 42-43 0.4244646929534426 2210 44-45 0.3339126472479492 2210 46-47 0.2979066249505493 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-1.3796454957725288 2216 84-85 -1.459801840885067 2216 86-87 -1.4392990765508458 2216 88-89 -1.937074345804394 2216 90-91 -1.7467153375340807 2216 92-93 -1.541013289518368 2216 94-95 -1.5049665009013218 2216 96-97 -1.4196982395292927 2216 98-99 -1.4709697878567702 2216 100-101 -1.5077413890240123 2216 102-103 -1.7176855991567592 2216 104-105 -1.6892882810848242 2216 106-107 -1.3494028507062161 2216 108-109 -1.4549449054359336 2216 110-111 -1.3647916984477924 2216 112-113 -1.6320096537180717 2216 114-115 -1.4954320765778242 2216 116-117 -1.4148928057454704 2216 118-119 -1.3807834838205366 2216 120-121 -1.3207925943176555 2216 122-123 -1.199654803359536 2216 124-125 -1.2081019719863733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34729.0 3 7094.0 4 4680.0 5 3994.0 6 4310.0 7 5098.0 8 5981.0 9 6383.0 10 7029.0 11 7603.0 12 8170.0 13 8694.0 14 8746.0 15 8351.0 16 8004.0 17 7320.0 18 7169.0 19 6966.0 20 7010.0 21 7774.0 22 8974.0 23 11739.0 24 16448.0 25 22597.0 26 27495.0 27 31393.0 28 37448.0 29 44938.0 30 53929.0 31 64793.0 32 81581.0 33 105699.0 34 143516.0 35 208655.0 36 371887.0 37 589045.0 38 212845.0 39 401.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.69983123772809 17.955631957118815 12.290282033760649 25.054254771392447 2 15.936297747805527 19.56371635285866 39.14512676497104 25.354859134364776 3 19.043778744199987 23.769028427277487 29.162555885024567 28.02463694349796 4 12.719336550148105 15.699666310051455 36.78672408899369 34.79427305080675 5 13.67683407764155 37.98523695201463 34.66978278451026 13.668146185833551 6 33.671460580527835 35.80666114164227 17.027840118772865 13.494038159057023 7 29.393610517774032 31.922894280068846 20.780099777665377 17.90339542449174 8 26.706261013381173 35.056007336037645 19.843599752918177 18.394131897663 9 26.392907453659966 15.818132279755485 19.57691620153406 38.212044065050485 10-11 25.139778910272653 26.11396660954659 27.868168619924642 20.878085860256117 12-13 25.694968805209502 23.46480055894078 27.588831009568587 23.251399626281135 14-15 22.709094209013013 25.212143018624005 24.628104361401185 27.450658410961797 16-17 23.171980380553222 28.29913697541565 25.597232753723 22.931649890308133 18-19 23.31150263772092 27.365174623198158 26.621886180289412 22.701436558791503 20-21 23.610523490421404 26.632182809933898 26.82873624398921 22.928557455655486 22-23 23.545583572536586 26.61124429665146 26.578241071387954 23.264931059424 24-25 23.377138244423627 26.82839842885506 26.530232075730474 23.264231250990836 26-27 23.07012928998208 27.029869550017732 26.388071012937186 23.511930147063005 28-29 23.285901212940978 27.143480666841192 26.34214674791302 23.228471372304806 30-31 23.237443848875795 26.790171085207763 26.39316660039689 23.579218465519546 32-33 22.973757312568317 26.9024731616632 26.374530860732676 23.74923866503581 34-35 23.726450582270697 26.82659513787366 26.21271143190103 23.234242847954615 36-37 23.695306133727666 26.539708993800478 26.184872198939672 23.580112673532184 38-39 23.203106325889028 26.79267485307473 26.40913739779025 23.595081423246 40-41 23.52678425222065 26.529596225714773 26.53403115641159 23.409588365652986 42-43 23.331115082634007 26.500628403282526 26.324639094553433 23.843617419530037 44-45 23.267958248188794 26.70878691634059 26.596799816235627 23.42645501923499 46-47 23.679042417336742 26.9465680973321 26.021258134983583 23.353131350347574 48-49 23.396292246050606 26.869434366004462 26.135484862931197 23.598788525013738 50-51 23.37344898139278 26.9279301162576 26.325763261726514 23.37285764062311 52-53 23.56038185419269 26.667791765188603 26.258070913959074 23.51375546665963 54-55 23.193729030866972 26.747191776631297 26.35298174219819 23.706097450303538 56-57 23.194395984862716 27.097673136418866 26.288176989202018 23.419753889516397 58-59 23.270780565381312 26.599073649504344 26.41989006070134 23.710255724413003 60-61 22.99360672681153 26.70911107390977 26.64808520239755 23.64919699688115 62-63 23.097539539060488 26.72886435575192 26.752224313826822 23.421371791360773 64-65 23.0887871235221 26.732165675068448 26.459819233290403 23.719227968119043 66-67 22.931146760913283 26.91248698653833 26.382508997684244 23.77385725486414 68-69 23.168543389428407 26.469149225332973 26.845160989490275 23.517146395748345 70-71 23.200051674191492 26.455184579438722 26.50108181466478 23.843681931705003 72-73 23.050928108892407 26.64206024320281 26.4255428232061 23.881468824698683 74-75 23.09545223157685 26.8737725747305 26.346545512940367 23.68422968075229 76-77 23.339860941476694 26.62527207844047 26.476729190690314 23.55813778939252 78-79 23.199278169166252 26.592856567737144 26.568883486732243 23.63898177636436 80-81 23.36898668185482 26.68906070592174 26.4482959725303 23.493656639693132 82-83 23.216627011387057 26.7462046940326 26.5018129211004 23.535355373479945 84-85 23.166667955601795 26.749172162458596 26.56520320972142 23.518956672218184 86-87 23.384236391573303 26.674552148244018 26.46555655766884 23.47565490251384 88-89 23.236166909218785 26.73885757724781 26.35728723159853 23.66768828193488 90-91 23.14557769777938 26.85504780304776 26.501992390725576 23.497382108447287 92-93 23.25795093028392 26.422446998915746 26.715084812879887 23.604517257920453 94-95 23.09987954372181 26.316330559684992 26.872963206247714 23.710826690345485 96-97 23.331054288130453 26.04491493156723 26.847197979883447 23.77683280041887 98-99 23.142399069843396 26.298895143357065 26.664125354614242 23.894580432185293 100-101 22.988074631923958 26.138619041012074 27.044382931006638 23.828923396057327 102-103 22.877820536936657 25.988119074919158 27.063097824863146 24.07096256328104 104-105 22.731965480935294 26.273237944404755 26.847213721194024 24.14758285346593 106-107 22.900051697123544 26.25122163737277 26.63311622664219 24.2156104388615 108-109 22.956918350423738 26.11843484850621 26.667491506349826 24.257155294720224 110-111 22.94767310153962 26.311106501559816 26.85592817106045 23.885292225840114 112-113 22.94709136353515 26.394476250739494 26.958460202807682 23.699972182917673 114-115 22.84558280474049 26.645127934745105 26.685865616600413 23.823423643913994 116-117 22.8340270669851 26.41608097350165 26.936699647446833 23.813192312066416 118-119 22.759975776930787 26.546186450625214 26.68922745776518 24.00461031467882 120-121 22.662328592299115 26.680385804105093 26.827093825341702 23.83019177825409 122-123 22.760057945852292 26.7547547356266 26.761670383827575 23.72351693469353 124-125 22.761654877484446 26.764194319764567 26.72886096667495 23.745289836076036 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 453.0 1 391.0 2 289.5 3 223.0 4 177.5 5 152.0 6 150.5 7 146.5 8 136.5 9 130.5 10 119.5 11 121.5 12 124.5 13 131.0 14 143.5 15 160.0 16 201.0 17 247.0 18 324.5 19 382.5 20 500.5 21 769.5 22 1126.5 23 1609.5 24 2375.0 25 3726.5 26 5574.0 27 7990.5 28 10939.5 29 14339.0 30 18578.5 31 23784.0 32 31062.0 33 35426.0 34 37317.0 35 41748.5 36 47773.5 37 53923.5 38 58607.5 39 62241.5 40 64451.5 41 67326.0 42 70240.5 43 72283.5 44 74952.0 45 79592.0 46 82312.0 47 83044.5 48 84097.0 49 84385.0 50 84609.5 51 83878.5 52 84142.0 53 85580.5 54 87561.0 55 82691.5 56 73663.5 57 67792.0 58 63168.5 59 55969.0 60 45150.5 61 36941.5 62 30946.5 63 23633.5 64 17076.5 65 12732.5 66 9385.0 67 7423.0 68 5769.0 69 4248.5 70 3072.5 71 1976.5 72 1417.0 73 1060.5 74 785.0 75 587.5 76 400.0 77 254.0 78 165.0 79 103.0 80 56.5 81 42.0 82 28.0 83 19.5 84 16.5 85 11.0 86 9.5 87 6.5 88 4.5 89 6.0 90 6.0 91 6.0 92 6.5 93 3.5 94 2.0 95 1.5 96 1.5 97 0.5 98 0.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08674143320318327 2 4.548580660890576E-4 3 4.5485806608905755E-5 4 0.0018194322643562304 5 0.0011826309718315497 6 2.7291483965343453E-4 7 0.0 8 0.0011826309718315497 9 5.003438726979633E-4 10-11 0.008869732288736623 12-13 0.002001375490791853 14-15 0.004048236788192612 16-17 0.0025926909767076283 18-19 0.012895226173624782 20-21 0.01628391876598826 22-23 0.009915905840741455 24-25 0.009938648744045908 26-27 0.009665733904392473 28-29 0.006982071314467034 30-31 0.01828529425678011 32-33 0.0021378329106185707 34-35 0.0011826309718315497 36-37 5.68572582611322E-4 38-39 2.9565774295788744E-4 40-41 0.0014555458114849842 42-43 0.0026836625899254395 44-45 9.324590354825681E-4 46-47 0.0016147461346161544 48-49 0.008005501963167412 50-51 0.004275665821237141 52-53 0.007368700670642733 54-55 0.007618872606991714 56-57 0.009415561968043492 58-59 0.008369388416038659 60-61 0.010916593586137382 62-63 0.012826997463711423 64-65 0.008414874222647566 66-67 0.012895226173624782 68-69 0.012986197786842592 70-71 0.004480351950977218 72-73 0.012804254560406972 74-75 0.013145398109973764 76-77 0.006049612278984466 78-79 0.008596817449083189 80-81 0.012917969076929235 82-83 0.017193634898166377 84-85 0.013236369723191576 86-87 0.016170204249465997 88-89 0.015237745213983429 90-91 0.011348708748921986 92-93 0.009438304871347945 94-95 0.008187445189603036 96-97 0.009051675515172245 98-99 0.006754642281422504 100-101 0.010598192939875042 102-103 0.011758081008402138 104-105 0.010211563583699342 106-107 0.004844238403848463 108-109 0.0036843503353213665 110-111 0.0070048142177714864 112-113 0.009028932611867792 114-115 0.012622311333971348 116-117 0.01082562197291957 118-119 0.2143063778378595 120-121 0.02660919686620987 122-123 0.026108852993511903 124-125 0.23165921305915702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2198488.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.61067886973947 #Duplication Level Percentage of deduplicated Percentage of total 1 85.6850936449202 62.21652818574855 2 8.748629738309479 12.704878889572848 3 2.562933012987985 5.582889179121732 4 1.1319603153095559 3.287696277929248 5 0.5553824802922941 2.016334946319159 6 0.3234842798093208 1.4093047896386128 7 0.1919387458521704 0.975576184641274 8 0.14724970328531345 0.8553520735131455 9 0.10158524886844042 0.6638556495140003 >10 0.5005660125687749 6.693636989954086 >50 0.03556274618869472 1.7994976587518907 >100 0.015614071607750803 1.794449175295465 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.5485806608905755E-5 0.0 7 0.0 0.0 0.0 4.5485806608905755E-5 0.0 8 0.0 0.0 0.0 4.5485806608905755E-5 0.0 9 0.0 0.0 0.0 4.5485806608905755E-5 0.0 10-11 0.0 0.0 0.0 4.5485806608905755E-5 0.0 12-13 0.0 0.0 0.0 4.5485806608905755E-5 4.5485806608905755E-5 14-15 0.0 0.0 0.0 4.5485806608905755E-5 4.5485806608905755E-5 16-17 0.0 0.0 0.0 4.5485806608905755E-5 4.5485806608905755E-5 18-19 0.0 0.0 0.0 4.5485806608905755E-5 4.5485806608905755E-5 20-21 0.0 0.0 0.0 4.5485806608905755E-5 4.5485806608905755E-5 22-23 0.0 0.0 0.0 1.5920032313117014E-4 4.5485806608905755E-5 24-25 0.0 0.0 0.0 1.8194322643562302E-4 4.5485806608905755E-5 26-27 0.0 0.0 0.0 4.5485806608905755E-4 4.5485806608905755E-5 28-29 0.0 0.0 0.0 0.002797377106447704 4.5485806608905755E-5 30-31 0.0 0.0 0.0 0.010643678746483948 4.5485806608905755E-5 32-33 0.0 0.0 0.0 0.02806474267769485 4.5485806608905755E-5 34-35 0.0 0.0 0.0 0.05476491115712253 4.5485806608905755E-5 36-37 0.0 0.0 0.0 0.09756705517610284 4.5485806608905755E-5 38-39 0.0 0.0 0.0 0.16190672862440003 4.5485806608905755E-5 40-41 0.0 0.0 0.0 0.23209132822194162 4.5485806608905755E-5 42-43 0.0 0.0 0.0 0.3022759278194832 4.5485806608905755E-5 44-45 0.0 0.0 0.0 0.38853976005327295 4.5485806608905755E-5 46-47 0.0 0.0 0.0 0.49236111363810037 4.5485806608905755E-5 48-49 0.0 0.0 0.0 0.6037331111200062 4.5485806608905755E-5 50-51 0.0 0.0 0.0 0.724907299926131 4.5485806608905755E-5 52-53 0.0 0.0 0.0 0.8413964506515388 4.5485806608905755E-5 54-55 0.0 0.0 0.0 0.9695299678688262 4.5485806608905755E-5 56-57 0.0 0.0 0.0 1.1237495951763212 9.097161321781151E-5 58-59 0.0 4.5485806608905755E-5 0.0 1.2915467357565746 1.3645741982671727E-4 60-61 0.0 4.5485806608905755E-5 0.0 1.4733989905789797 1.3645741982671727E-4 62-63 0.0 4.5485806608905755E-5 0.0 1.6524766111982419 1.3645741982671727E-4 64-65 0.0 4.5485806608905755E-5 0.0 1.8392413331344089 1.3645741982671727E-4 66-67 0.0 4.5485806608905755E-5 0.0 2.0586421213124657 1.3645741982671727E-4 68-69 0.0 4.5485806608905755E-5 0.0 2.301149699247847 1.3645741982671727E-4 70-71 0.0 4.5485806608905755E-5 0.0 2.5658543508083738 1.3645741982671727E-4 72-73 0.0 4.5485806608905755E-5 0.0 2.8380641604593704 1.3645741982671727E-4 74-75 0.0 4.5485806608905755E-5 0.0 3.1076357933270504 1.3645741982671727E-4 76-77 0.0 4.5485806608905755E-5 0.0 3.4040213091906804 1.3645741982671727E-4 78-79 0.0 4.5485806608905755E-5 0.0 3.739820276480927 1.3645741982671727E-4 80-81 0.0 4.5485806608905755E-5 0.0 4.0999086645003295 1.3645741982671727E-4 82-83 0.0 4.5485806608905755E-5 0.0 4.4796696638780835 1.3645741982671727E-4 84-85 0.0 4.5485806608905755E-5 0.0 4.857906888734439 1.3645741982671727E-4 86-87 0.0 4.5485806608905755E-5 0.0 5.257090327534196 1.3645741982671727E-4 88-89 0.0 4.5485806608905755E-5 0.0 5.68593051224296 1.3645741982671727E-4 90-91 0.0 4.5485806608905755E-5 0.0 6.159574216461495 1.3645741982671727E-4 92-93 0.0 4.5485806608905755E-5 0.0 6.639472219088756 1.3645741982671727E-4 94-95 0.0 4.5485806608905755E-5 0.0 7.125783720447871 1.5920032313117014E-4 96-97 0.0 4.5485806608905755E-5 0.0 7.625922907016095 2.2742903304452878E-4 98-99 0.0 4.5485806608905755E-5 0.0 8.148577567855726 2.2742903304452878E-4 100-101 0.0 4.5485806608905755E-5 0.0 8.73115977890259 2.2742903304452878E-4 102-103 0.0 4.5485806608905755E-5 0.0 9.34480879586334 2.2742903304452878E-4 104-105 0.0 4.5485806608905755E-5 0.0 9.954045689583022 2.2742903304452878E-4 106-107 0.0 4.5485806608905755E-5 0.0 10.561031035875565 2.2742903304452878E-4 108-109 0.0 4.5485806608905755E-5 0.0 11.197013583881287 2.7291483965343453E-4 110-111 0.0 4.5485806608905755E-5 0.0 11.870567408145963 2.7291483965343453E-4 112-113 0.0 4.5485806608905755E-5 0.0 12.580282448664718 2.7291483965343453E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2700 0.0 49.168415 1 TCAACGC 2705 0.0 47.053978 4 ATCAACG 2740 0.0 46.234806 3 CAACGCA 2855 0.0 44.375484 5 GGTATCA 1570 0.0 44.364067 1 AACGCAG 2950 0.0 42.744816 6 TATCAAC 3150 0.0 40.21694 2 GTACATG 7465 0.0 35.80648 1 TACATGG 7530 0.0 34.9114 2 ACGCAGA 3725 0.0 34.17095 7 ACATGGG 7630 0.0 33.596394 3 CGCAGAG 3855 0.0 32.710037 8 CATGGGG 5055 0.0 28.23839 4 CATGGGA 3665 0.0 24.180323 4 GAGTACA 5450 0.0 23.047886 1 TAAGGTG 1030 0.0 22.521482 5 GCAGAGT 5615 0.0 21.187006 9 GTATATA 1245 0.0 20.561 1 GTGTAGC 1365 0.0 20.497946 1 ATGGGGA 3035 0.0 20.38188 5 >>END_MODULE