FastQCFastQC Report
Thu 26 May 2016
SRR1364446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364446_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2198488
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.064.033741
GTATCAA44350.051.4405361
TCAACGC53200.042.4170154
CAACGCA53750.042.0640685
ATCAACG54550.041.4764253
AACGCAG56750.039.83776
TATCAAC56850.039.590742
GTACATG72350.034.084911
TACATGG74300.032.53642
ACGCAGA69800.032.30437
CGCAGAG71650.031.3871678
ACATGGG74700.031.2448163
TAAGGTG11550.030.3931855
TGGTATC14250.029.24932
GTAAGGT11950.026.905334
GTGGTAT17300.026.5121521
TACCGTA1358.254773E-526.4419847
CATGGGG48700.025.796814
AGGTAAG12550.025.6201862
GCAGAGT90850.024.6884029
Produced by FastQC (version 0.11.5)