FastQCFastQC Report
Thu 26 May 2016
SRR1364445_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364445_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838985
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTG856.219125E-848.98515
GTATCAA19700.046.2267761
CAACGCA20200.043.58085
TCAACGC20200.043.2816284
ATCAACG20700.041.9477163
AACGCAG21850.040.28986
GGTATCA13600.039.3887331
GTACATG56500.038.2407651
TATCAAC24050.036.846562
TACATGG60000.034.990432
ACATGGG57950.034.8940433
ACGCAGA27000.032.1642887
CGCAGAG28450.030.5249828
CATGGGG39550.029.4745524
CATGGGA27500.024.4389954
TAAGGTG8150.022.625025
AGGACCG4350.021.87845
GCAGAGT40250.021.13279
GAGTACA45000.020.8983971
ATGGGGA24750.020.1877965
Produced by FastQC (version 0.11.5)