FastQCFastQC Report
Thu 26 May 2016
SRR1364445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364445_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838985
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22740.1236551684760887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22640.12311139025060021No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24800.066.3141561
GTATCAA40800.052.1383061
TCAACGC49350.042.5955734
CAACGCA50150.041.881895
ATCAACG50200.041.8743363
TATCAAC51950.040.580582
AACGCAG52600.040.040976
GTACATG58700.032.8893851
ACGCAGA64750.032.6193777
CGCAGAG64750.032.6193778
TACATGG61800.031.0290152
ACATGGG64400.029.2198623
GCAGAGT75500.027.8172919
GAGTACT47400.025.79411312-13
GTACTTT48350.025.10271814-15
CAGAGTA73900.024.83692610-11
CATGGGG42050.023.933034
GAGTACA48750.022.979051
CATGGGA29200.022.6369084
AGAGTAC73450.022.51853210-11
Produced by FastQC (version 0.11.5)