FastQCFastQC Report
Thu 26 May 2016
SRR1364444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683382
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.052.5634961
GTATCAA30200.050.2706381
TCAACGC33600.044.7840654
ATCAACG33500.044.738883
CAACGCA33600.044.436645
AACGCAG35100.042.3669136
GTACATG87300.039.8953931
TATCAAC37950.039.6495362
TACATGG93400.036.8689772
ACATGGG91800.035.503183
ACGCAGA43900.034.0097247
CGCAGAG44250.033.8751458
CATGGGA70850.030.8083554
CATGGGG27550.030.2237764
TAAGGTG9200.027.8027295
GCAGAGT52950.027.6352279
TCTACGG900.00955027926.4330252
GGTAAGG9050.025.629813
AGGTAAG8600.025.5877822
TAAGGTA5800.024.6107884
Produced by FastQC (version 0.11.5)