##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364444_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1683382 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.679660944455865 31.0 31.0 34.0 27.0 34.0 2 30.673715175759273 31.0 31.0 34.0 27.0 34.0 3 30.618146683284007 31.0 31.0 34.0 26.0 34.0 4 34.16078228233402 37.0 35.0 37.0 30.0 37.0 5 34.363843738379046 37.0 35.0 37.0 30.0 37.0 6 34.49051908598286 37.0 35.0 37.0 31.0 37.0 7 34.34839329397605 37.0 35.0 37.0 30.0 37.0 8 34.20398875596864 37.0 35.0 37.0 30.0 37.0 9 35.29159988641913 39.0 35.0 39.0 28.0 39.0 10-11 35.514955607224024 39.0 35.0 39.0 29.5 39.0 12-13 35.57338946240366 39.0 35.0 39.0 30.0 39.0 14-15 36.718483683442024 40.0 36.0 41.0 30.0 41.0 16-17 36.87234121548169 40.0 37.0 41.0 30.0 41.0 18-19 36.876790888817865 40.0 36.5 41.0 30.0 41.0 20-21 36.85443351538747 40.0 37.0 41.0 30.0 41.0 22-23 36.78095583771241 40.0 36.5 41.0 30.0 41.0 24-25 36.84898585110213 40.0 36.5 41.0 30.0 41.0 26-27 36.867891542145514 40.0 36.0 41.0 30.0 41.0 28-29 36.82319105229829 40.0 36.0 41.0 30.0 41.0 30-31 36.599532963997476 40.0 36.0 41.0 30.0 41.0 32-33 36.67552848967139 40.0 36.0 41.0 30.0 41.0 34-35 36.628203818265845 40.0 36.0 41.0 30.0 41.0 36-37 36.50516638528866 40.0 36.0 41.0 30.0 41.0 38-39 36.36008968849613 40.0 35.5 41.0 29.0 41.0 40-41 36.3276769622106 40.0 35.0 41.0 29.0 41.0 42-43 36.190608548742944 40.0 35.0 41.0 29.0 41.0 44-45 36.17552759860804 40.0 35.0 41.0 29.0 41.0 46-47 36.035695106636524 39.0 35.0 41.0 28.0 41.0 48-49 35.877364733613646 39.0 35.0 41.0 28.0 41.0 50-51 35.7514470868763 39.0 35.0 41.0 27.5 41.0 52-53 35.62348831103101 39.0 35.0 41.0 28.0 41.0 54-55 35.49682484427183 39.0 34.0 40.0 28.0 41.0 56-57 35.46894317510821 39.0 34.5 40.5 28.0 41.0 58-59 35.449070086290575 39.0 34.0 41.0 28.0 41.0 60-61 35.27272627365625 38.0 34.0 40.5 28.0 41.0 62-63 35.024909675878675 38.0 34.0 40.0 28.0 41.0 64-65 34.72862962773749 37.5 34.0 40.0 27.0 41.0 66-67 34.430827940419945 37.0 34.0 40.0 26.0 41.0 68-69 34.137880172177205 36.5 33.5 39.5 26.0 41.0 70-71 33.782725192499385 36.0 33.0 39.0 26.0 41.0 72-73 33.45285769955957 35.5 33.0 39.0 26.0 40.5 74-75 33.09422697878438 35.0 33.0 37.5 26.0 40.0 76-77 32.73333147200101 35.0 33.0 37.0 26.0 39.0 78-79 32.39100810154795 35.0 32.0 37.0 25.0 39.0 80-81 32.051620784824834 35.0 32.0 36.0 25.0 38.5 82-83 31.73960723115728 35.0 32.0 36.0 24.0 37.0 84-85 31.37330475198143 35.0 32.0 35.5 22.5 37.0 86-87 31.161003563065307 35.0 32.0 35.0 22.5 36.5 88-89 30.994964898044532 35.0 32.0 35.0 21.5 36.0 90-91 30.845640502274588 35.0 32.0 35.0 20.0 36.0 92-93 30.6024895121844 35.0 31.0 35.0 20.0 36.0 94-95 30.50421116538017 34.0 31.0 35.0 19.5 35.0 96-97 30.40920331808229 34.0 31.0 35.0 19.0 35.0 98-99 30.236792956084834 34.0 31.0 35.0 17.5 35.0 100-101 30.085893754358786 34.0 31.0 35.0 15.0 35.0 102-103 29.024707107477685 33.5 29.0 34.5 10.0 35.0 104-105 29.971084697353305 34.0 30.5 35.0 15.0 35.0 106-107 30.19816981528851 35.0 31.0 35.0 15.0 35.0 108-109 30.17167434367244 35.0 31.0 35.0 9.0 35.0 110-111 30.0428155938462 35.0 31.0 35.0 6.0 35.0 112-113 29.908470269968433 35.0 31.0 35.0 2.0 35.0 114-115 29.777568014865313 35.0 31.0 35.0 2.0 35.0 116-117 29.633730787189123 35.0 31.0 35.0 2.0 35.0 118-119 29.348169340054724 34.0 31.0 35.0 2.0 35.0 120-121 29.19542207294601 34.0 31.0 35.0 2.0 35.0 122-123 28.934191407535543 34.0 30.0 35.0 2.0 35.0 124-125 27.73202487611249 33.5 27.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.725650477966326 1101 2 0.7452624773743572 1101 3 0.8510629222712645 1101 4 0.6935745733343808 1101 5 0.7251815796792727 1101 6 0.617019617597613 1101 7 0.6834644303340909 1101 8 0.8072849870121175 1101 9 1.279356552824673 1101 10-11 0.9248025358565783 1101 12-13 0.8075019585634777 1101 14-15 1.00661736993316 1101 16-17 0.6970099764062425 1101 18-19 0.8540178758984283 1101 20-21 0.9949247455870989 1101 22-23 1.0390061017466792 1101 24-25 0.8989840659316073 1101 26-27 0.9086224257229603 1101 28-29 0.9783943864966247 1101 30-31 1.0530209206353263 1101 32-33 0.9209565804835478 1101 34-35 0.9826918334448465 1101 36-37 0.9288698669783386 1101 38-39 1.10590015990973 1101 40-41 0.9493859023435007 1101 42-43 0.9628877817986847 1101 44-45 0.814293486469353 1101 46-47 0.6924387104623051 1101 48-49 0.739112999479147 1101 50-51 0.7798665680691954 1101 52-53 0.6356144955040435 1101 54-55 0.5731059022785701 1101 56-57 0.6280992442971254 1101 58-59 0.7495441555886089 1101 60-61 0.8050508539250032 1101 62-63 0.8074778599705823 1101 64-65 0.6753866279007426 1101 66-67 0.8021964223692493 1101 68-69 0.7799401102233077 1101 70-71 0.7045064004998949 1101 72-73 0.7114773074592122 1101 74-75 0.7709071590484129 1101 76-77 0.8498930493440895 1101 78-79 0.7381216296256099 1101 80-81 0.7918165101752166 1101 82-83 0.8171630859586472 1101 84-85 0.9109590669172505 1101 86-87 0.8727613255820152 1101 88-89 0.7334308814804231 1101 90-91 0.649554435222246 1101 92-93 0.7437427510593686 1101 94-95 0.7276457178576905 1101 96-97 0.5632540408842992 1101 98-99 0.6898660336345976 1101 100-101 0.7295551837795777 1101 102-103 0.7319442038889967 1101 104-105 0.6833982881802179 1101 106-107 0.5789726227626417 1101 108-109 0.6212491299355847 1101 110-111 0.7272024012816942 1101 112-113 0.6402939672982697 1101 114-115 0.7738417663291521 1101 116-117 0.7648044694253073 1101 118-119 0.6249971195400548 1101 120-121 0.5209851799080916 1101 122-123 0.6135116448443085 1101 124-125 0.3362353330418131 1102 1 0.26582245950291394 1102 2 0.20501755112146824 1102 3 0.3429751215898271 1102 4 0.24982754914723415 1102 5 0.21802216629626514 1102 6 0.13339673794946805 1102 7 0.17466265561124317 1102 8 0.3258696060513344 1102 9 0.5333239717355696 1102 10-11 0.37343368773027663 1102 12-13 0.2564203947289698 1102 14-15 0.33479505913590657 1102 16-17 0.12805128359912743 1102 18-19 0.15324498610434745 1102 20-21 0.2771973669593706 1102 22-23 0.25748657930910923 1102 24-25 0.19772590957025926 1102 26-27 0.21025965882818554 1102 28-29 0.11721356037634223 1102 30-31 0.14185439610033512 1102 32-33 0.04616603865756019 1102 34-35 -0.1357615440502542 1102 36-37 -0.02476381880323686 1102 38-39 -0.04310600148730259 1102 40-41 -0.07825410325040849 1102 42-43 -0.14512462793960168 1102 44-45 -0.1229744259162402 1102 46-47 -0.05707443881750862 1102 48-49 -0.16530473884855468 1102 50-51 -0.07118658416671764 1102 52-53 -0.1976510508201983 1102 54-55 -0.2639071604254468 1102 56-57 -0.18053102929271603 1102 58-59 -0.11122206455324601 1102 60-61 -0.19336300374379078 1102 62-63 -0.1976043973545032 1102 64-65 -0.23174914844693006 1102 66-67 -0.17506442543108136 1102 68-69 -0.1814623352077831 1102 70-71 -0.04569692368660583 1102 72-73 -0.03323040600981386 1102 74-75 0.011249168087374528 1102 76-77 -0.007383171370996422 1102 78-79 -0.140331326411129 1102 80-81 0.04902451652834117 1102 82-83 -0.09338215590172183 1102 84-85 0.0016686215403893812 1102 86-87 -0.03767545706380204 1102 88-89 -0.008696210025114226 1102 90-91 -0.03951797471350105 1102 92-93 -0.013249294724566596 1102 94-95 -0.049816457359646904 1102 96-97 -0.12770277422265508 1102 98-99 -0.12560520175859224 1102 100-101 -0.17840840457585116 1102 102-103 -0.2502961154998111 1102 104-105 -0.18553626655713273 1102 106-107 -0.256829631332252 1102 108-109 -0.16338071099630724 1102 110-111 -0.23830631590663387 1102 112-113 -0.17564337746395253 1102 114-115 -0.2972716691886781 1102 116-117 -0.33395611879499043 1102 118-119 -0.2506935158762147 1102 120-121 -0.4868328726860831 1102 122-123 -0.44246148851145506 1102 124-125 -0.717363412957468 1103 1 0.38055382677135796 1103 2 0.5040228128574178 1103 3 0.5458304969371 1103 4 0.4888706526618307 1103 5 0.47864169078955854 1103 6 0.38225402514678564 1103 7 0.42050478905125743 1103 8 0.5032916737245543 1103 9 0.9321696209945074 1103 10-11 0.5600412038020224 1103 12-13 0.5039585898695123 1103 14-15 0.5720564191742028 1103 16-17 0.5336118649137731 1103 18-19 0.4681579444320718 1103 20-21 0.5163402565561412 1103 22-23 0.49019428483109806 1103 24-25 0.4220736834506269 1103 26-27 0.479654022928365 1103 28-29 0.3922180443389891 1103 30-31 0.6891730618601031 1103 32-33 0.533876888610223 1103 34-35 0.5399228278201207 1103 36-37 0.4961414785479832 1103 38-39 0.6220901969571173 1103 40-41 0.6690695857543147 1103 42-43 0.5889763115278868 1103 44-45 0.5121369852822184 1103 46-47 0.47329877018070476 1103 48-49 0.4023129653595561 1103 50-51 0.43029308579552605 1103 52-53 0.5044894549225134 1103 54-55 0.41689058819538616 1103 56-57 0.39945947028784445 1103 58-59 0.4789225551214926 1103 60-61 0.4367350430586683 1103 62-63 0.4081084350451576 1103 64-65 0.42757294579065075 1103 66-67 0.29034570592601483 1103 68-69 0.31419994946961793 1103 70-71 0.4243250141666408 1103 72-73 0.38218274985363365 1103 74-75 0.41559527056971035 1103 76-77 0.4401513732431894 1103 78-79 0.37553428353375296 1103 80-81 0.36505578796292326 1103 82-83 0.3281328095216125 1103 84-85 0.43452420354969234 1103 86-87 0.533686601084856 1103 88-89 0.4266878140345689 1103 90-91 0.3419759081658462 1103 92-93 0.3811888719207559 1103 94-95 0.43129470855964414 1103 96-97 0.45584887416347186 1103 98-99 0.3964481257942367 1103 100-101 0.36447170384176175 1103 102-103 0.260660139880752 1103 104-105 0.3749522764469013 1103 106-107 0.4369514707375224 1103 108-109 0.38978151221045465 1103 110-111 0.3840425836029766 1103 112-113 0.3791256807349406 1103 114-115 0.27603836714914465 1103 116-117 0.22424684775547732 1103 118-119 0.2183351620253262 1103 120-121 0.09956505490105272 1103 122-123 0.2758457956010183 1103 124-125 0.08966486097260074 1104 1 0.45211003664472926 1104 2 0.44274603413416713 1104 3 0.5333550179716298 1104 4 0.4444965194275099 1104 5 0.4547388951057201 1104 6 0.3544586653308599 1104 7 0.3776130872056882 1104 8 0.45252823375791706 1104 9 0.9637036904607967 1104 10-11 0.6357967718780557 1104 12-13 0.5405704671610323 1104 14-15 0.6294814989795441 1104 16-17 0.49116541092411836 1104 18-19 0.6061318684608992 1104 20-21 0.5966911789587428 1104 22-23 0.7416493837958242 1104 24-25 0.7674233974322462 1104 26-27 0.713744836717936 1104 28-29 0.673587471211988 1104 30-31 0.8268250198451739 1104 32-33 0.8283050386434994 1104 34-35 0.8069235574471776 1104 36-37 0.6380124649320891 1104 38-39 0.7811476743382997 1104 40-41 0.7086383205108646 1104 42-43 0.7306546367250206 1104 44-45 0.5950229995438718 1104 46-47 0.5048597527451335 1104 48-49 0.6169883041939315 1104 50-51 0.5433970961521126 1104 52-53 0.4643832206138825 1104 54-55 0.41733659846201476 1104 56-57 0.5209766828532949 1104 58-59 0.5204867474350223 1104 60-61 0.6549605473742943 1104 62-63 0.6701316138556237 1104 64-65 0.576260646352317 1104 66-67 0.48950278464263874 1104 68-69 0.4956010163871696 1104 70-71 0.6071508742231089 1104 72-73 0.49012950918877607 1104 74-75 0.4382629608998343 1104 76-77 0.49024007972954564 1104 78-79 0.4357708290813136 1104 80-81 0.5742387962678634 1104 82-83 0.46747876786806586 1104 84-85 0.5159327420922217 1104 86-87 0.4628400431023785 1104 88-89 0.5613839056885368 1104 90-91 0.41455817037566334 1104 92-93 0.42600200124314824 1104 94-95 0.4977159298043716 1104 96-97 0.3669687868826941 1104 98-99 0.43594717621169465 1104 100-101 0.46698521947766025 1104 102-103 0.45092697600590625 1104 104-105 0.39457604200865504 1104 106-107 0.3170145830962774 1104 108-109 0.2257185035395537 1104 110-111 0.25148894229129226 1104 112-113 0.3135176467558196 1104 114-115 0.38433095598333367 1104 116-117 0.3730911474774281 1104 118-119 0.22935291170911398 1104 120-121 0.2109547847713138 1104 122-123 0.23326729207601105 1104 124-125 -0.06268530816821638 1105 1 0.13008563785705363 1105 2 0.2825562657855798 1105 3 0.440115705767667 1105 4 0.2101623078190471 1105 5 0.3130957083377197 1105 6 0.2820116283671581 1105 7 0.3440068793743194 1105 8 0.43042692243730585 1105 9 0.8598397875685819 1105 10-11 0.390647211269453 1105 12-13 0.4398995766509728 1105 14-15 0.037864584314377225 1105 16-17 0.17781156758200467 1105 18-19 0.24728350037731417 1105 20-21 0.4724108061233281 1105 22-23 0.4971581487785386 1105 24-25 0.47668069650777056 1105 26-27 0.5056290414728082 1105 28-29 0.5750389097048085 1105 30-31 0.6795847329081184 1105 32-33 0.5594329114408296 1105 34-35 0.49486123545018756 1105 36-37 0.5109110774604488 1105 38-39 0.569559773212788 1105 40-41 0.4576715832775804 1105 42-43 0.43944969909271236 1105 44-45 0.42591061586456647 1105 46-47 0.4961355108285659 1105 48-49 0.5059285104109037 1105 50-51 0.4121797459030958 1105 52-53 0.2884172448908231 1105 54-55 0.20262269785432352 1105 56-57 0.31241768865600505 1105 58-59 0.3888498050508815 1105 60-61 0.33431410134252104 1105 62-63 0.3926391908823845 1105 64-65 0.370901137374112 1105 66-67 0.23950468841820793 1105 68-69 0.23610993367192634 1105 70-71 0.23195554684986064 1105 72-73 0.18967641060366702 1105 74-75 0.14868080156142582 1105 76-77 0.11240726168480109 1105 78-79 0.0986571051439995 1105 80-81 0.047246685513819386 1105 82-83 0.1095602037717498 1105 84-85 0.21467777323799098 1105 86-87 0.1663357557998033 1105 88-89 0.12205418699041104 1105 90-91 0.10808381042501125 1105 92-93 0.11703636058459566 1105 94-95 0.05874792846411481 1105 96-97 0.08258964617083464 1105 98-99 0.0409758089962331 1105 100-101 -0.0911755048708649 1105 102-103 -0.08377047598087373 1105 104-105 -0.12250767842570909 1105 106-107 -0.14411488887252943 1105 108-109 -0.08470428693852483 1105 110-111 -0.05839469894601024 1105 112-113 -0.0064697121744110575 1105 114-115 -0.07855227403844722 1105 116-117 -0.2586699210737251 1105 118-119 -0.4689561548316483 1105 120-121 -0.3927099831965215 1105 122-123 -0.2519609325390455 1105 124-125 -0.32764136902811103 1106 1 0.34587479782295816 1106 2 0.47587139927428623 1106 3 0.5287809379050934 1106 4 0.3328631369451571 1106 5 0.3090588596130033 1106 6 0.24200077998815317 1106 7 0.2786939751678901 1106 8 0.47010606076605654 1106 9 0.8901097453075906 1106 10-11 0.4093500848977669 1106 12-13 0.5244558362252647 1106 14-15 0.7004992204466376 1106 16-17 0.5354489456122522 1106 18-19 0.5604221092813333 1106 20-21 0.6097058343469968 1106 22-23 0.5918549604280301 1106 24-25 0.5225897549042386 1106 26-27 0.6307437839430179 1106 28-29 0.5914137033173787 1106 30-31 0.6148345138543121 1106 32-33 0.5408528271572663 1106 34-35 0.5327405561747653 1106 36-37 0.6244599279495162 1106 38-39 0.6542799506016621 1106 40-41 0.6513623865940872 1106 42-43 0.6696289059689207 1106 44-45 0.48979624295451174 1106 46-47 0.5661020417911544 1106 48-49 0.515833765914067 1106 50-51 0.5866988706415768 1106 52-53 0.5902882922362522 1106 54-55 0.43263038709000057 1106 56-57 0.44165690137364066 1106 58-59 0.5587986030862595 1106 60-61 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-0.7302117879910703 2216 124-125 -0.5691830010267012 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25390.0 3 5023.0 4 3233.0 5 2808.0 6 3317.0 7 3841.0 8 4350.0 9 4799.0 10 5331.0 11 5970.0 12 6343.0 13 6829.0 14 6699.0 15 6684.0 16 6360.0 17 6143.0 18 5792.0 19 5520.0 20 5714.0 21 5967.0 22 7163.0 23 9071.0 24 13108.0 25 18366.0 26 21553.0 27 23368.0 28 27356.0 29 32829.0 30 39683.0 31 47992.0 32 60697.0 33 79480.0 34 108211.0 35 157536.0 36 272224.0 37 453387.0 38 184898.0 39 347.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.749773319547195 19.36121597043314 12.649215195111669 24.239795514907993 2 15.083201851517428 21.20684861849046 39.743230270599085 23.96671925939303 3 17.876058881535958 26.474652187860137 30.12047190770949 25.528817022894412 4 11.856459523424801 16.97757558763984 38.511736163255996 32.65422872567936 5 12.484955701744486 40.09713893981212 34.66622152573243 12.751683832710967 6 30.623443233452758 38.289462194149024 18.03315597159757 13.053938600800652 7 27.531956501851628 33.88375306377281 21.859447231822603 16.724843202552954 8 26.174186461932912 35.6665282527711 20.748480565916797 17.410804719379193 9 26.843949933763817 16.15693519547099 20.210767686248417 36.78834718451677 10-11 23.879406144295682 27.666856681322255 28.533121515015864 19.920615659366202 12-13 24.708235524053286 25.32035354805979 28.193696384351192 21.777714543535737 14-15 21.957539863000964 26.670375658714907 25.518558376376355 25.853526101907775 16-17 21.969662315936553 29.91753197106431 26.42663095943193 21.686174753567208 18-19 22.171790852305996 28.60444922913676 27.77706977798414 21.44669014057311 20-21 22.844467893650325 28.02384095021569 27.452741878608172 21.67894927752581 22-23 22.54937024714829 28.14995247148289 27.29571649239544 22.004960788973385 24-25 22.231156979056728 28.27349505210198 27.407312129739402 22.088035839101895 26-27 22.036458002242163 28.432708708487482 27.18778609990393 22.343047189366423 28-29 22.236091006819304 28.31877754552019 27.33968161930899 22.10544982835151 30-31 22.228427863235726 27.98759281105536 27.32439463559304 22.459584690115868 32-33 21.84368576758112 28.40890729145627 27.144397447930903 22.603009493031713 34-35 22.55989943939602 28.32630276416911 26.888199396089853 22.225598400345024 36-37 22.80624841092759 27.81779666051235 26.84771993356192 22.528234994998133 38-39 22.229377156380988 28.015428519831577 27.10758024351066 22.647614080276774 40-41 22.519478945630038 27.767566694151448 27.36895820016681 22.343996160051706 42-43 22.425734780865252 27.8301575750375 27.126163990910847 22.6179436531864 44-45 22.10463002566296 27.998467351012263 27.35350727117194 22.543395352152835 46-47 22.51294297960311 28.141230704341652 26.818150765291726 22.527675550763508 48-49 22.156392824955457 28.14579529866215 26.915922607996684 22.78188926838571 50-51 22.352608843009012 28.006168891830228 27.071003148903234 22.57021911625753 52-53 22.68638698556384 27.80330891908663 27.029592063156826 22.480712032192702 54-55 22.051135659834657 27.902569377835512 27.19823838534103 22.848056576988803 56-57 22.099720114948333 28.117129903855236 27.14760238142908 22.635547599767346 58-59 22.147299330090327 27.55811507997804 27.335742615903403 22.958842974028226 60-61 21.787608763523124 27.881960442174535 27.530007904673383 22.800422889628955 62-63 21.879108781064836 27.83042100653959 27.607800453083627 22.68266975931195 64-65 21.782309683904597 27.76014629051595 27.398133108685347 23.05941091689411 66-67 21.769898570287843 27.943731225521883 27.20721914331343 23.07915106087684 68-69 21.991515240454497 27.491280410876012 27.66604352412506 22.85116082454443 70-71 21.9909914352098 27.322227285067846 27.431299594186846 23.255481685535504 72-73 21.869284311509162 27.523770283128307 27.31508313472159 23.29186227064094 74-75 21.867616085459574 27.82566460800827 27.26067724811141 23.046042058420745 76-77 22.014181886014992 27.487147071718564 27.467690940393542 23.030980101872895 78-79 22.07873837987325 27.436762301108136 27.46839785947574 23.016101459542874 80-81 22.105069909659733 27.67802849026208 27.230530619471445 22.986370980606743 82-83 22.061001803257746 27.438779845104023 27.441928861927494 23.058289489710738 84-85 22.051911069178615 27.515338252545778 27.40420591772999 23.028544760545618 86-87 22.105452971511056 27.553879275131504 27.232654909135956 23.10801284422148 88-89 21.9925096258764 27.456139543049744 27.350619558568788 23.200731272505063 90-91 22.01016662765261 27.620883175276887 27.331842481436635 23.037107715633866 92-93 22.134324536435507 27.027602665625793 27.505173160520364 23.332899637418333 94-95 21.820690515584573 26.965901968530837 27.66532896476131 23.548078551123282 96-97 22.049705400520132 26.780318051588697 27.684063408323645 23.485913139567526 98-99 21.967774284539686 26.79640362821491 27.39022439896106 23.845597688284347 100-101 21.640492648035174 26.87593999368461 27.753084248683823 23.730483109596396 102-103 21.772925533289587 26.597625955679238 27.824870043664852 23.804578467366323 104-105 21.447863097557963 26.955876481715606 27.59124967917946 24.005010741546972 106-107 21.544673789093814 26.893029033588995 27.476831615996083 24.085465561321104 108-109 21.685796122702186 26.743802320604352 27.352127042439854 24.2182745142536 110-111 21.630565869597504 27.032021387197386 27.450586662706073 23.886826080499034 112-113 21.47386750680693 27.08573078479169 27.69634941786859 23.744052290532796 114-115 21.42920375276345 27.156410099498967 27.4833301943459 23.93105595339168 116-117 21.293445910559864 27.21093911757603 27.633059158296795 23.86255581356731 118-119 21.174898928592476 27.35321800288495 27.271420262383817 24.20046280613876 120-121 21.230910559125054 27.521000538940054 27.276784043096193 23.971304858838707 122-123 21.303368276126804 27.44826098621214 27.319851196763196 23.928519540897863 124-125 21.235998510199504 27.46862829686091 27.289429472505233 24.005943720434352 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 832.0 1 700.0 2 482.5 3 372.0 4 321.0 5 264.5 6 221.5 7 219.0 8 230.5 9 214.5 10 191.0 11 175.0 12 174.5 13 185.5 14 214.5 15 232.5 16 259.0 17 331.5 18 425.0 19 497.5 20 644.5 21 923.0 22 1317.0 23 1942.5 24 2688.0 25 3915.5 26 5721.5 27 7973.5 28 10743.5 29 14458.0 30 19416.0 31 24535.5 32 31142.5 33 35577.0 34 38901.0 35 42678.0 36 46928.0 37 52698.0 38 56154.5 39 58207.5 40 59087.5 41 60603.0 42 61962.5 43 63949.0 44 66855.0 45 68761.5 46 67013.0 47 64016.5 48 62467.5 49 59667.0 50 57527.0 51 55201.5 52 52880.0 53 52145.0 54 51669.0 55 49080.5 56 44401.5 57 40090.5 58 37783.5 59 33338.0 60 26164.0 61 21050.5 62 17635.0 63 13474.5 64 9539.5 65 7136.5 66 5139.0 67 3849.5 68 2633.0 69 1461.5 70 1021.5 71 766.0 72 590.5 73 492.0 74 376.5 75 286.0 76 201.0 77 120.0 78 78.5 79 39.5 80 22.0 81 19.0 82 19.0 83 13.5 84 5.5 85 7.0 86 7.5 87 6.0 88 4.5 89 4.0 90 5.5 91 4.0 92 3.5 93 3.5 94 2.5 95 3.0 96 2.0 97 0.5 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08904693052438484 2 3.5642533899019947E-4 3 1.1880844633006648E-4 4 0.0022573604802712632 5 0.001188084463300665 6 2.9702111582516626E-4 7 0.0 8 0.0014851055791258313 9 7.128506779803989E-4 10-11 0.010603653834958435 12-13 0.0027622963771740464 14-15 0.005079061080610343 16-17 0.0037424660593970947 18-19 0.014049098778530365 20-21 0.018504415515907858 22-23 0.01081156861603605 24-25 0.0114353129592689 26-27 0.010989781285531151 28-29 0.008643314470512338 30-31 0.019336074640218325 32-33 0.0024949773729313965 34-35 0.0011286802401356316 36-37 5.940422316503325E-4 38-39 3.5642533899019947E-4 40-41 0.0015445098022908643 42-43 0.003386040720406895 44-45 0.0013068929096307315 46-47 0.0021979562571062304 48-49 0.009445271483240286 50-51 0.005791911758590742 52-53 0.00852450602418227 54-55 0.009118548255832604 56-57 0.01134620662452135 58-59 0.010098717938055652 60-61 0.011910546744589166 62-63 0.01479165156809328 64-65 0.009267058813745187 66-67 0.01461343889859818 68-69 0.014643141010180697 70-71 0.005643401200678158 72-73 0.014286715671190496 74-75 0.014672843121763212 76-77 0.006415656101823591 78-79 0.008880931363172471 80-81 0.014583736787015663 82-83 0.01883113874331554 84-85 0.015058970572335927 86-87 0.01725692682944216 88-89 0.01713811838311209 90-91 0.01354416288162758 92-93 0.010395739053880818 94-95 0.008732420805259888 96-97 0.00977199471064797 98-99 0.007722549011454322 100-101 0.0114353129592689 102-103 0.013098631207889832 104-105 0.011762036186676582 106-107 0.006385953990241075 108-109 0.004187997733134844 110-111 0.007841357457784389 112-113 0.010158122161220686 114-115 0.01479165156809328 116-117 0.012563993199404532 118-119 0.21557792586590566 120-121 0.026672496201099925 122-123 0.029018963016118744 124-125 0.23538923429144423 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1683382.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.789074597369854 #Duplication Level Percentage of deduplicated Percentage of total 1 83.77863158333196 53.44161379734729 2 9.014095903760659 11.500016720656694 3 2.9932370884821995 5.728074717744156 4 1.346598918928847 3.435931955691593 5 0.7183852054965053 2.2912563731531725 6 0.46767964233528175 1.7899710955557924 7 0.28273107623715393 1.2624607599160533 8 0.205952967907687 1.0510039386730532 9 0.1491008448276688 0.8559904421918727 >10 0.9173758179966944 10.882458933942814 >50 0.09261111709004632 4.074741323559575 >100 0.03338983464543511 3.612000186973373 >500 2.099989598550969E-4 0.07447975459455171 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.910633474754987E-5 0.0 16-17 0.0 0.0 0.0 1.188084463300665E-4 0.0 18-19 0.0 0.0 0.0 1.188084463300665E-4 0.0 20-21 0.0 0.0 0.0 1.7821266949509973E-4 0.0 22-23 0.0 0.0 0.0 4.455316737377494E-4 0.0 24-25 0.0 0.0 0.0 7.128506779803989E-4 0.0 26-27 5.940422316503325E-5 0.0 0.0 0.0015445098022908645 0.0 28-29 5.940422316503325E-5 0.0 0.0 0.00493055052269776 0.0 30-31 5.940422316503325E-5 0.0 0.0 0.017464841610519775 0.0 32-33 5.940422316503325E-5 0.0 0.0 0.044077933588454674 0.0 34-35 5.940422316503325E-5 0.0 0.0 0.08295799764996893 0.0 36-37 5.940422316503325E-5 0.0 0.0 0.15005506771487398 0.0 38-39 5.940422316503325E-5 0.0 0.0 0.24771561059818864 0.0 40-41 5.940422316503325E-5 0.0 0.0 0.35595010520487924 0.0 42-43 5.940422316503325E-5 0.0 0.0 0.461956941442881 0.0 44-45 5.940422316503325E-5 0.0 0.0 0.5936561041997598 0.0 46-47 5.940422316503325E-5 0.0 0.0 0.7527109117241363 0.0 48-49 5.940422316503325E-5 0.0 0.0 0.9177358436765987 0.0 50-51 5.940422316503325E-5 0.0 0.0 1.0991563412226102 0.0 52-53 5.940422316503325E-5 0.0 0.0 1.2676267181186445 0.0 54-55 5.940422316503325E-5 0.0 0.0 1.4546312126421692 0.0 56-57 5.940422316503325E-5 0.0 0.0 1.6745159446875397 0.0 58-59 5.940422316503325E-5 0.0 0.0 1.9166772604197977 0.0 60-61 5.940422316503325E-5 0.0 0.0 2.180788436611536 0.0 62-63 5.940422316503325E-5 0.0 0.0 2.4373255743497317 0.0 64-65 5.940422316503325E-5 0.0 0.0 2.705892067278847 0.0 66-67 5.940422316503325E-5 0.0 0.0 3.0147643256254373 0.0 68-69 5.940422316503325E-5 0.0 0.0 3.3542000567904373 0.0 70-71 5.940422316503325E-5 0.0 0.0 3.7374761046512317 0.0 72-73 5.940422316503325E-5 0.0 0.0 4.128147978296073 0.0 74-75 5.940422316503325E-5 0.0 0.0 4.504057902484403 0.0 76-77 5.940422316503325E-5 0.0 0.0 4.914630190889531 0.0 78-79 5.940422316503325E-5 0.0 0.0 5.372636751491937 0.0 80-81 5.940422316503325E-5 0.0 0.0 5.863137422165616 0.0 82-83 5.940422316503325E-5 0.0 0.0 6.375825570191436 0.0 84-85 5.940422316503325E-5 0.0 0.0 6.887919675985605 0.0 86-87 5.940422316503325E-5 0.0 0.0 7.408419479357626 0.0 88-89 5.940422316503325E-5 0.0 0.0 7.976917895046995 0.0 90-91 5.940422316503325E-5 0.0 0.0 8.587949734522526 0.0 92-93 5.940422316503325E-5 0.0 0.0 9.220515604895382 0.0 94-95 5.940422316503325E-5 2.9702111582516625E-5 0.0 9.867219680381517 0.0 96-97 5.940422316503325E-5 5.940422316503325E-5 0.0 10.526636259624969 0.0 98-99 5.940422316503325E-5 5.940422316503325E-5 0.0 11.203874105817931 0.0 100-101 5.940422316503325E-5 5.940422316503325E-5 0.0 11.956466209095737 0.0 102-103 5.940422316503325E-5 5.940422316503325E-5 0.0 12.740394040093097 0.0 104-105 5.940422316503325E-5 5.940422316503325E-5 0.0 13.523965445751468 0.0 106-107 5.940422316503325E-5 5.940422316503325E-5 0.0 14.305190384594821 0.0 108-109 5.940422316503325E-5 5.940422316503325E-5 0.0 15.110830459159002 0.0 110-111 5.940422316503325E-5 5.940422316503325E-5 0.0 15.960399956753726 0.0 112-113 5.940422316503325E-5 5.940422316503325E-5 0.0 16.848760412075215 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1665 0.0 52.563496 1 GTATCAA 3020 0.0 50.270638 1 TCAACGC 3360 0.0 44.784065 4 ATCAACG 3350 0.0 44.73888 3 CAACGCA 3360 0.0 44.43664 5 AACGCAG 3510 0.0 42.366913 6 GTACATG 8730 0.0 39.895393 1 TATCAAC 3795 0.0 39.649536 2 TACATGG 9340 0.0 36.868977 2 ACATGGG 9180 0.0 35.50318 3 ACGCAGA 4390 0.0 34.009724 7 CGCAGAG 4425 0.0 33.875145 8 CATGGGA 7085 0.0 30.808355 4 CATGGGG 2755 0.0 30.223776 4 TAAGGTG 920 0.0 27.802729 5 GCAGAGT 5295 0.0 27.635227 9 TCTACGG 90 0.009550279 26.433025 2 GGTAAGG 905 0.0 25.62981 3 AGGTAAG 860 0.0 25.587782 2 TAAGGTA 580 0.0 24.610788 4 >>END_MODULE