FastQCFastQC Report
Thu 26 May 2016
SRR1364444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364444_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683382
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31900.18949947189645608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27810.16520314462195748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17200.1021752638438572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28400.074.4736561
GTATCAA55700.055.621261
ATCAACG68750.044.953943
TCAACGC70150.043.97194
CAACGCA71450.043.3050655
TATCAAC73400.042.2708132
AACGCAG73950.042.001996
GTACATG90250.035.120211
ACGCAGA88600.035.056977
CGCAGAG89050.034.8088578
TGGTATC12800.032.5676962
TACATGG96100.032.3478972
ACATGGG95350.031.4762483
GTGGTAT15000.031.3782351
GCAGAGT100000.030.81889
TAAGGTG10700.028.917265
CATGGGA72350.028.478084
AGGTAAG9300.028.175312
GTAAGGT10900.026.7696514
GAGTACT55850.026.73846612-13
Produced by FastQC (version 0.11.5)