##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364444_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1683382 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.900679703121455 31.0 31.0 34.0 30.0 34.0 2 31.815985914070602 33.0 31.0 34.0 30.0 34.0 3 32.03791593351954 33.0 31.0 34.0 30.0 34.0 4 35.742922283831 37.0 35.0 37.0 33.0 37.0 5 35.6905776585469 37.0 35.0 37.0 33.0 37.0 6 35.75704742001518 37.0 35.0 37.0 33.0 37.0 7 35.659335195457714 37.0 35.0 37.0 33.0 37.0 8 35.66991092930779 37.0 35.0 37.0 33.0 37.0 9 37.2962916319647 39.0 37.0 39.0 33.0 39.0 10-11 37.245215286845166 39.0 37.0 39.0 33.0 39.0 12-13 37.22262326673328 39.0 37.0 39.0 33.0 39.0 14-15 38.48736204854276 40.0 38.0 41.0 33.0 41.0 16-17 38.510211882983185 40.0 38.0 41.0 33.5 41.0 18-19 38.58792626985438 40.0 38.0 41.0 34.0 41.0 20-21 38.596150784551575 40.0 38.0 41.0 34.0 41.0 22-23 38.568041300192114 40.0 38.0 41.0 34.0 41.0 24-25 38.51650397830082 40.0 38.0 41.0 34.0 41.0 26-27 38.39904638400553 40.0 38.0 41.0 34.0 41.0 28-29 38.31286006384766 40.0 38.0 41.0 33.5 41.0 30-31 38.183993294451284 40.0 37.5 41.0 33.0 41.0 32-33 38.03900837718355 40.0 37.0 41.0 33.0 41.0 34-35 37.93716161869379 40.0 37.0 41.0 33.0 41.0 36-37 37.8199160974752 40.0 37.0 41.0 33.0 41.0 38-39 37.711663187559324 40.0 37.0 41.0 33.0 41.0 40-41 37.58975027652666 40.0 37.0 41.0 32.0 41.0 42-43 37.441157146743876 40.0 37.0 41.0 32.0 41.0 44-45 37.25845945839981 40.0 36.0 41.0 31.0 41.0 46-47 37.019797051411985 40.0 36.0 41.0 31.0 41.0 48-49 36.77361377275034 40.0 36.0 41.0 30.0 41.0 50-51 36.56373479103377 39.5 35.5 41.0 30.0 41.0 52-53 36.43205641975499 39.0 35.0 41.0 30.0 41.0 54-55 36.27764702248212 39.0 35.0 41.0 30.0 41.0 56-57 36.084217664202185 39.0 35.0 41.0 29.0 41.0 58-59 35.87251289368664 38.5 35.0 40.5 29.0 41.0 60-61 35.64623715829205 38.0 35.0 40.0 28.0 41.0 62-63 35.6957315689487 38.0 35.0 40.0 29.0 41.0 64-65 35.49034384352453 38.0 35.0 40.0 29.0 41.0 66-67 35.22330166296182 37.0 34.0 40.0 29.0 41.0 68-69 34.90609261593625 37.0 34.0 39.5 28.0 41.0 70-71 34.56518692726903 36.0 34.0 39.0 28.0 41.0 72-73 34.21888436492727 36.0 34.0 39.0 28.0 41.0 74-75 33.88170480615808 35.0 34.0 38.5 28.0 40.0 76-77 33.55030765447177 35.0 33.5 37.0 27.5 39.0 78-79 33.212223666404896 35.0 33.0 37.0 27.0 39.0 80-81 32.91498126984844 35.0 33.0 36.0 26.0 39.0 82-83 32.63322258405994 35.0 33.0 36.0 26.0 37.0 84-85 32.394022271831346 35.0 33.0 35.5 26.0 37.0 86-87 32.19439586499084 35.0 33.0 35.0 26.0 36.5 88-89 32.00518331549226 35.0 33.0 35.0 26.0 36.0 90-91 31.873507617403536 35.0 33.0 35.0 25.5 36.0 92-93 31.744784606227228 35.0 33.0 35.0 25.0 36.0 94-95 31.62731988342515 35.0 32.0 35.0 25.0 35.5 96-97 31.56685054253877 35.0 32.0 35.0 25.0 35.0 98-99 31.482850594814487 35.0 32.0 35.0 25.0 35.0 100-101 31.466556313421435 35.0 32.5 35.0 25.0 35.0 102-103 30.33502110632049 33.5 30.0 34.5 22.0 35.0 104-105 31.30436941823068 34.5 32.0 35.0 24.5 35.0 106-107 31.487559864605895 35.0 33.0 35.0 25.0 35.0 108-109 31.488823095411497 35.0 33.0 35.0 25.0 35.0 110-111 31.424795738578645 35.0 33.0 35.0 25.0 35.0 112-113 31.357609265157876 35.0 33.0 35.0 24.5 35.0 114-115 31.234125409443607 35.0 33.0 35.0 24.0 35.0 116-117 31.1388205410299 35.0 33.0 35.0 24.0 35.0 118-119 31.025497480666896 35.0 33.0 35.0 23.0 35.0 120-121 30.88623378413218 35.0 33.0 35.0 21.5 35.0 122-123 30.686023433777954 35.0 32.5 35.0 18.5 35.0 124-125 29.64751910142796 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29902686035799775 1101 2 0.5026580768400883 1101 3 0.40209363913538 1101 4 0.29276305014234083 1101 5 0.27048866477461786 1101 6 0.2322889257445908 1101 7 0.33174514004059574 1101 8 0.30917659042979295 1101 9 0.31058314826847067 1101 10-11 0.354691038476183 1101 12-13 0.38329363837131325 1101 14-15 0.408849713007271 1101 16-17 0.3719562459319974 1101 18-19 0.20850569370616512 1101 20-21 0.17847658251646692 1101 22-23 0.2817414004634884 1101 24-25 0.33181090959224946 1101 26-27 0.3163926470903604 1101 28-29 0.3486922160067749 1101 30-31 0.25903995228526355 1101 32-33 0.2739304976964618 1101 34-35 0.3479461725981352 1101 36-37 0.26432599542490465 1101 38-39 0.31946539516961536 1101 40-41 0.22017366177401243 1101 42-43 0.18954526854822262 1101 44-45 0.18885597694348633 1101 46-47 0.1637611331388058 1101 48-49 0.06574511821273887 1101 50-51 0.13861724840272416 1101 52-53 0.16602469484406157 1101 54-55 0.0396512280356589 1101 56-57 -0.01259980233976421 1101 58-59 -0.012680524034877294 1101 60-61 -0.07835688543907793 1101 62-63 0.13425652929502263 1101 64-65 0.20586643331964893 1101 66-67 0.06153915448750524 1101 68-69 0.22402673055790245 1101 70-71 0.2406189937918981 1101 72-73 0.26812035438419457 1101 74-75 0.25085661535216985 1101 76-77 0.31158382816921204 1101 78-79 0.2915585637449638 1101 80-81 0.12788633444241526 1101 82-83 0.1403828261438278 1101 84-85 0.0664623118602492 1101 86-87 0.12820856804626857 1101 88-89 0.17104920240427646 1101 90-91 0.11644419229445901 1101 92-93 0.10258386331475577 1101 94-95 0.18313476089943848 1101 96-97 0.16418347108292508 1101 98-99 0.13989756548098597 1101 100-101 0.16761530719000106 1101 102-103 0.3015140544441195 1101 104-105 0.257204308197732 1101 106-107 0.24712184652589642 1101 108-109 0.24191099927805126 1101 110-111 0.33228746565534806 1101 112-113 0.3405585247054539 1101 114-115 0.23047956953254456 1101 116-117 0.3035767265438558 1101 118-119 0.25499835880401633 1101 120-121 0.3076400793669265 1101 122-123 0.22961835522662 1101 124-125 0.16007952517901103 1102 1 0.17765363112782495 1102 2 0.35566180026260596 1102 3 0.20993529929228316 1102 4 0.16006290641914944 1102 5 0.143244638467948 1102 6 0.1955943440928536 1102 7 0.2800898181193574 1102 8 0.28206841813113215 1102 9 0.2298875769264015 1102 10-11 0.24190156031679066 1102 12-13 0.22414654041421755 1102 14-15 0.3080432096984538 1102 16-17 0.28386024891698014 1102 18-19 0.23405100703761406 1102 20-21 0.2828879547598362 1102 22-23 0.2516055066674525 1102 24-25 0.28041384143933357 1102 26-27 0.2737392926133779 1102 28-29 0.32131201149336164 1102 30-31 0.28813045251065716 1102 32-33 0.2603600542471085 1102 34-35 0.22002685505416508 1102 36-37 0.14338387683624632 1102 38-39 0.1603682532909545 1102 40-41 0.11910278912974093 1102 42-43 0.18133434602744103 1102 44-45 0.2696469658690006 1102 46-47 0.21314485344016987 1102 48-49 0.28333876486057363 1102 50-51 0.3023368748491464 1102 52-53 0.1620086092815356 1102 54-55 0.035965014690198416 1102 56-57 0.08815092404961433 1102 58-59 -0.04518807897072463 1102 60-61 0.015909129352344564 1102 62-63 0.05451439697212379 1102 64-65 0.13934254672495427 1102 66-67 0.044665862701720016 1102 68-69 0.02396980420657968 1102 70-71 0.06815771675243099 1102 72-73 0.029115959870281927 1102 74-75 0.1305458738079679 1102 76-77 0.16844949539011367 1102 78-79 0.08289644499715365 1102 80-81 0.10248219492554966 1102 82-83 0.14954387201485986 1102 84-85 -0.05778493919233085 1102 86-87 -0.005485097449302145 1102 88-89 0.005681762184686079 1102 90-91 0.0067718113302923655 1102 92-93 -0.07857625534787616 1102 94-95 -0.062044588053495886 1102 96-97 0.015422669186182958 1102 98-99 -0.06780750385259893 1102 100-101 -0.12930881306356312 1102 102-103 -0.0510578215215709 1102 104-105 -0.16882584434808834 1102 106-107 -0.2830838973621077 1102 108-109 -0.25712458315456743 1102 110-111 -0.2403471250827991 1102 112-113 -0.17377960662185288 1102 114-115 -0.16994956644391834 1102 116-117 -0.11499928649247337 1102 118-119 -0.3069830613662461 1102 120-121 -0.24804908536597026 1102 122-123 -0.3371748373661454 1102 124-125 -0.49617505689123576 1103 1 0.24810290683775094 1103 2 0.3706635025845024 1103 3 0.2100012199542789 1103 4 0.11294260603814621 1103 5 0.1266504317326067 1103 6 0.08430352249663997 1103 7 0.16970634842679289 1103 8 0.2076299618572719 1103 9 0.25144468933591924 1103 10-11 0.2882526857423855 1103 12-13 0.14243539087704704 1103 14-15 0.15324377067272366 1103 16-17 0.3261267822941818 1103 18-19 0.14674489788175293 1103 20-21 0.198588551862251 1103 22-23 0.11975641399672554 1103 24-25 0.13522124524985912 1103 26-27 0.18826870431596632 1103 28-29 0.10215763933185684 1103 30-31 0.20531486790073217 1103 32-33 0.19296809436549012 1103 34-35 0.20065583471244253 1103 36-37 0.14359544143223246 1103 38-39 0.059592757796117724 1103 40-41 0.04796956769560268 1103 42-43 0.0719681211498866 1103 44-45 0.08113989387926779 1103 46-47 0.08792403053610087 1103 48-49 0.09643264478329883 1103 50-51 0.0944715291903151 1103 52-53 -0.05131759194905072 1103 54-55 0.008608769627649338 1103 56-57 -0.12251552177528424 1103 58-59 -0.3951072469644714 1103 60-61 -0.33222527820117165 1103 62-63 -0.1433850871421285 1103 64-65 -0.04463576070094888 1103 66-67 -0.06293377894330376 1103 68-69 0.05509996945625062 1103 70-71 0.061580735372679385 1103 72-73 0.16073734718796118 1103 74-75 0.16015692229193945 1103 76-77 0.11885128710465409 1103 78-79 0.13739141202249527 1103 80-81 0.20092685950779554 1103 82-83 0.07578219617587223 1103 84-85 0.09946103131292716 1103 86-87 0.033620789084793046 1103 88-89 0.008709511766419098 1103 90-91 -0.07915011477189182 1103 92-93 -0.05133110998225732 1103 94-95 0.061860084333634546 1103 96-97 0.0526028276401469 1103 98-99 -0.019789053307952997 1103 100-101 -0.09456040296584334 1103 102-103 -0.018740707235505027 1103 104-105 -0.05751269721875474 1103 106-107 0.007755876057867539 1103 108-109 -0.03640003211274134 1103 110-111 -0.07366318629760116 1103 112-113 -0.025229506692465264 1103 114-115 -0.05890553249764707 1103 116-117 -0.012486849435767056 1103 118-119 -0.24491672164796086 1103 120-121 -0.3569995726131907 1103 122-123 -0.31906520964592033 1103 124-125 -0.34978465059519337 1104 1 0.3467487891718619 1104 2 0.40665264280632485 1104 3 0.3685664849796737 1104 4 0.20090970649109607 1104 5 0.22237124912837913 1104 6 0.267458057811119 1104 7 0.3004033671721871 1104 8 0.21821923672082022 1104 9 0.2913928759039166 1104 10-11 0.3025785355063064 1104 12-13 0.23586173323452897 1104 14-15 0.21979963364921673 1104 16-17 0.3499624858353485 1104 18-19 0.3741004991988959 1104 20-21 0.3653256398650342 1104 22-23 0.3557486801024652 1104 24-25 0.37063118205362855 1104 26-27 0.3485357462920149 1104 28-29 0.38057165732017495 1104 30-31 0.33921012334262457 1104 32-33 0.20438298378925168 1104 34-35 0.1584161465079248 1104 36-37 0.24136325534913539 1104 38-39 0.09549658425923724 1104 40-41 0.06788106706012798 1104 42-43 0.05118602499312175 1104 44-45 0.011117044430221767 1104 46-47 0.13231829141322748 1104 48-49 -0.08567815026621872 1104 50-51 0.018462688275690198 1104 52-53 -0.10762307162357132 1104 54-55 0.02218471221507201 1104 56-57 0.030119255284382973 1104 58-59 4.5552269065751716E-4 1104 60-61 -0.03333283224274908 1104 62-63 0.07856006655605796 1104 64-65 0.13352414579700422 1104 66-67 0.022832165542517657 1104 68-69 0.21616853381400603 1104 70-71 0.2626135731644652 1104 72-73 0.21222629718830177 1104 74-75 0.1440012868187992 1104 76-77 0.18798905395793497 1104 78-79 0.21348335570473864 1104 80-81 0.11073382473957594 1104 82-83 0.1252654723156965 1104 84-85 0.042572002479317916 1104 86-87 0.05103493592051933 1104 88-89 0.2762054271510266 1104 90-91 0.12265519755347043 1104 92-93 0.1418115393492272 1104 94-95 0.04246366964624926 1104 96-97 0.13624690687291618 1104 98-99 0.11636758854733031 1104 100-101 0.05456774226425054 1104 102-103 0.07876849696781818 1104 104-105 0.11655153893338976 1104 106-107 0.18249736164563402 1104 108-109 0.03629590553426354 1104 110-111 0.06738249049057998 1104 112-113 -0.035612015770869476 1104 114-115 -0.014253119353380583 1104 116-117 -0.09451637150336367 1104 118-119 -0.09883410224872335 1104 120-121 0.1323061685681992 1104 122-123 0.007915438082974902 1104 124-125 -0.164641976866573 1105 1 0.2502928577686987 1105 2 0.40115149292588015 1105 3 0.18135681075374777 1105 4 0.1343598047259178 1105 5 0.14733050833122974 1105 6 0.0750381306115031 1105 7 0.147458607124058 1105 8 0.12193540662898528 1105 9 0.24488135425418278 1105 10-11 0.16359796924730574 1105 12-13 0.2427800536475715 1105 14-15 0.19933252327592754 1105 16-17 0.2643062924726749 1105 18-19 0.22402625195174863 1105 20-21 0.2283379458703081 1105 22-23 0.3077126149781151 1105 24-25 0.3881748546651522 1105 26-27 0.29603913501252777 1105 28-29 0.2987176845213213 1105 30-31 0.2371460498721234 1105 32-33 0.24368186132317504 1105 34-35 0.23689624824569222 1105 36-37 0.1867359384542695 1105 38-39 0.26555514439570516 1105 40-41 0.29582811163894007 1105 42-43 0.2071619765001529 1105 44-45 0.25926467080284255 1105 46-47 0.22322814383254297 1105 48-49 0.06410596158440285 1105 50-51 0.152358269993357 1105 52-53 0.17796268204310906 1105 54-55 0.2507862748340628 1105 56-57 -0.018540105960838105 1105 58-59 0.1003782294026081 1105 60-61 0.10125496227133368 1105 62-63 0.33627111911550145 1105 64-65 0.28159562114691283 1105 66-67 0.19199252436613534 1105 68-69 0.31143270171178017 1105 70-71 0.3003890494891124 1105 72-73 0.19826438792986067 1105 74-75 0.2366637550257522 1105 76-77 0.2784777912216754 1105 78-79 0.20035149259381768 1105 80-81 0.3787283114055313 1105 82-83 0.4176477047590694 1105 84-85 0.3651953290605974 1105 86-87 0.3303854438478453 1105 88-89 0.4968610113055263 1105 90-91 0.3445006414377829 1105 92-93 0.3883778897353096 1105 94-95 0.20552052399980525 1105 96-97 0.28986217667599234 1105 98-99 0.2963764220659648 1105 100-101 0.3230945245789343 1105 102-103 0.25824160804169694 1105 104-105 0.2839109260699608 1105 106-107 0.2463313371859286 1105 108-109 0.29394984482666686 1105 110-111 0.35602684998432466 1105 112-113 0.41234827908588656 1105 114-115 0.27432346573015565 1105 116-117 0.3131394715646074 1105 118-119 0.32798248039797784 1105 120-121 0.27781478124886405 1105 122-123 0.167518082046481 1105 124-125 -0.02552053121555531 1106 1 0.21467619895022239 1106 2 0.38670526691003815 1106 3 0.17612991869512484 1106 4 0.06865379579686959 1106 5 0.03674082144406299 1106 6 0.10410165323514065 1106 7 0.12775926219925537 1106 8 0.06711294452379946 1106 9 0.15815154445845536 1106 10-11 0.1836711958055588 1106 12-13 0.24676564080117913 1106 14-15 0.2581983908184142 1106 16-17 0.13258342683163704 1106 18-19 0.19928554013119282 1106 20-21 0.21513704819674473 1106 22-23 0.1577224500469896 1106 24-25 0.19467464306215732 1106 26-27 0.13519954796620226 1106 28-29 0.10090405013554005 1106 30-31 0.167196804788162 1106 32-33 0.2026024178092527 1106 34-35 0.16328746344296263 1106 36-37 0.09597628369498779 1106 38-39 0.11828169371707986 1106 40-41 0.11482216291243219 1106 42-43 0.13753728868908155 1106 44-45 0.13045581685659613 1106 46-47 0.25258552639287757 1106 48-49 0.19433493986976202 1106 50-51 0.24289365537904217 1106 52-53 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-0.6992699541297256 2216 74-75 -0.6265726087108021 2216 76-77 -0.6011307671556949 2216 78-79 -0.7333956076855515 2216 80-81 -0.667730034555241 2216 82-83 -0.5817727643757777 2216 84-85 -0.6296266377351927 2216 86-87 -0.5536087870167563 2216 88-89 -0.533867624434464 2216 90-91 -0.5036418843084292 2216 92-93 -0.433552263502186 2216 94-95 -0.5880579378946997 2216 96-97 -0.5223286345845679 2216 98-99 -0.5126326862251744 2216 100-101 -0.5215176429519062 2216 102-103 -0.5025567862982676 2216 104-105 -0.5497757002256165 2216 106-107 -0.5737560814703642 2216 108-109 -0.40099430972224326 2216 110-111 -0.4642656843026316 2216 112-113 -0.3875840837249527 2216 114-115 -0.4214047842987725 2216 116-117 -0.4065660931439332 2216 118-119 -0.5085468363975316 2216 120-121 -0.6193001000347813 2216 122-123 -0.5296645741802735 2216 124-125 -0.4199377769079895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70.0 3 13.0 4 36.0 5 107.0 6 367.0 7 822.0 8 1429.0 9 2383.0 10 3629.0 11 4711.0 12 6126.0 13 8479.0 14 12051.0 15 9299.0 16 4977.0 17 4646.0 18 4875.0 19 4807.0 20 4797.0 21 4929.0 22 5258.0 23 6215.0 24 7856.0 25 10820.0 26 14217.0 27 19173.0 28 24509.0 29 31197.0 30 39534.0 31 48977.0 32 61276.0 33 77672.0 34 102857.0 35 147688.0 36 267427.0 37 509104.0 38 230453.0 39 596.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.508878735725084 19.83755598238991 13.110754799509353 24.54281048237565 2 15.261829356896698 21.580641730990067 38.93943471449678 24.218094197616455 3 18.103269037704127 26.44504773163357 30.688381045165354 24.76330218549695 4 12.383941974703111 17.387434788424667 37.986415184078716 32.24220805279351 5 12.133464425427508 39.67557677056012 34.989707686412665 13.201251117599707 6 30.59786786362216 38.54217284015155 17.452307319431952 13.407651976794336 7 26.96256479900575 33.95931281864833 22.07633658531369 17.001785797032227 8 26.39008852417336 35.10450984981424 20.516020724945378 17.98938090106702 9 27.108820220247097 14.971111726274843 20.163575468907236 37.75649258457082 10-11 24.171845724856272 27.064088840203826 28.505175889964367 20.25888954497553 12-13 25.249052205619403 24.732027549302536 28.58130240195036 21.437617843127704 14-15 22.09531169989937 25.91732001411444 26.45059172546695 25.536776560519243 16-17 21.381302639567252 29.494315609885334 27.243341083604317 21.881040666943093 18-19 21.586593167735995 27.451310027649722 29.68817804412015 21.273918760494137 20-21 22.303167076753823 26.813403018447385 29.600470956681253 21.28295894811754 22-23 21.949979267926114 27.08039529946263 29.443673509637147 21.52595192297411 24-25 21.660086658880754 27.22035164923945 29.511483430380032 21.608078261499767 26-27 21.484613198073273 27.457912662927946 29.335896438968273 21.721577700030505 28-29 21.553532900552224 27.2593882768912 29.549458944406954 21.63761987814962 30-31 21.628361628706173 26.888039667976145 29.509710563256036 21.97388814006165 32-33 21.394995313006792 27.213104334013316 29.438772661226032 21.953127691753863 34-35 21.993195840278677 27.12358216970361 29.159513408127207 21.723708581890506 36-37 22.37644218602789 26.668011182250968 28.947737352543868 22.007809279177277 38-39 21.838477541045346 26.91049922121063 29.09903990894521 22.15198332879881 40-41 22.040120686408404 26.72655047520719 29.30004734547524 21.933281492909167 42-43 22.121293937441692 26.730253268214092 28.97705114165098 22.171401652693238 44-45 21.848724769541317 26.91433079360478 29.018844207672412 22.21810022918149 46-47 22.372491805187416 27.122928723248798 28.314933865278352 22.189645606285442 48-49 22.190596073856085 27.340348180032812 28.018625600131163 22.450430145979936 50-51 22.33507377858198 27.241002707942318 28.092413989342287 22.33150952413342 52-53 22.69348252505967 27.08182100081859 28.045684223782835 22.1790122503389 54-55 22.013007148704215 27.208500506718025 28.161047225169334 22.61744511940843 56-57 22.11782876661469 27.4002227667632 27.995752580381673 22.48619588624044 58-59 22.021900626508685 26.912933618830966 28.274839288774672 22.790326465885677 60-61 21.724261246447615 27.22430995808423 28.476585147940803 22.574843647527352 62-63 21.776348637810543 27.16151397089596 28.58793453269014 22.47420285860336 64-65 21.572198110708086 27.070712411086728 28.493681172781937 22.86340830542325 66-67 21.594831119733964 27.364050465075664 28.20304601094701 22.838072404243363 68-69 21.92131673024899 26.862708523674367 28.539897658404335 22.676077087672315 70-71 21.85641761644119 26.75560270930781 28.27952894827199 23.10845072597901 72-73 21.833725203192145 26.988199945110498 28.063149065393354 23.114925786304 74-75 21.87144688490194 27.25444967333618 27.889748137974625 22.984355303787257 76-77 21.979948698512874 26.99918972639603 28.097841131721736 22.92302044336936 78-79 22.032254117009686 26.90007378004517 28.013487134827393 23.054184968117752 80-81 22.027650289714394 27.279815276627644 27.658131071854157 23.034403361803797 82-83 22.132558147823843 26.98724947753986 27.779078070218166 23.10111430441813 84-85 21.99512647753154 27.0250602655844 27.781394834921603 23.198418421962455 86-87 22.087707959334246 27.055148504617488 27.624448877319587 23.23269465872868 88-89 21.945226930072913 27.04430723388987 27.590647874338682 23.419817961698534 90-91 21.9414380939312 27.2058731802248 27.548190219564077 23.304498506279923 92-93 22.070810533216072 26.581231386030304 27.7467736775932 23.601184403160428 94-95 21.767861476192074 26.43588474012232 27.9113996050205 23.88485417866511 96-97 21.985942840475328 26.31019908913313 27.89202309810576 23.811834972285777 98-99 21.91140300848364 26.309528053040566 27.588014781017563 24.191054157458236 100-101 21.65701767832507 26.261171496508183 27.85280896072553 24.229001864441223 102-103 21.696856349903474 26.02947864463229 27.941886332641904 24.331778672822335 104-105 21.561170781550747 26.267643704108544 27.632713922486513 24.538471591854194 106-107 21.65137342153808 26.240386643338155 27.530158239786594 24.57808169533717 108-109 21.7829182181809 25.98151996161998 27.409720066481796 24.82584175371732 110-111 21.66313636413593 26.253509881134107 27.537013224450902 24.546340530279064 112-113 21.522690474429496 26.295738204906797 27.711217706236468 24.470353614427236 114-115 21.470930082316027 26.410010636657887 27.394280204796505 24.72477907622958 116-117 21.3712039216292 26.405711834865762 27.51814501996576 24.70493922353928 118-119 21.193476142952193 26.558787187529703 27.172470535120237 25.07526613439787 120-121 21.231098240142572 26.676735724363258 27.270810128462163 24.821355907032004 122-123 21.224111910860056 26.661817283190825 27.22773380033154 24.88633700561758 124-125 21.102841619645055 26.69343909989479 27.15923308744727 25.044486193012883 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 86.0 1 77.5 2 142.5 3 207.5 4 138.0 5 114.5 6 277.5 7 666.0 8 1131.5 9 1285.5 10 996.0 11 691.0 12 618.5 13 642.0 14 731.0 15 860.0 16 948.0 17 1034.5 18 1215.0 19 1410.0 20 1607.0 21 1888.5 22 2267.0 23 2822.5 24 3567.0 25 4780.5 26 6524.0 27 8780.5 28 11661.5 29 15490.0 30 20466.5 31 25596.0 32 31975.5 33 35902.0 34 38745.5 35 42254.5 36 46531.0 37 52170.5 38 55467.5 39 57346.0 40 58172.5 41 59472.0 42 60729.0 43 62430.5 44 65004.0 45 67516.5 46 66138.0 47 63170.0 48 61751.5 49 59246.5 50 57231.5 51 54895.5 52 52653.5 53 51984.0 54 52012.0 55 49386.5 56 44000.5 57 39443.0 58 37068.0 59 32698.5 60 26002.5 61 21088.0 62 17519.5 63 13235.0 64 9275.0 65 6817.0 66 4694.5 67 3599.0 68 2508.0 69 1267.0 70 845.0 71 658.5 72 476.5 73 402.0 74 344.0 75 262.0 76 159.0 77 76.0 78 40.0 79 15.5 80 6.0 81 3.5 82 3.0 83 2.0 84 1.5 85 1.0 86 1.5 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042176998447173604 2 0.005405784308018025 3 7.128506779803989E-4 4 0.08061153083495011 5 0.005168167415357893 6 0.0 7 0.005940422316503325 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 8.910633474754987E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.4553167373774933E-4 28-29 3.5642533899019947E-4 30-31 5.346380084852992E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.534464548153657E-4 42-43 5.049358969027826E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 2.970211158251662E-5 52-53 0.0 54-55 0.0 56-57 4.1582956215523273E-4 58-59 3.2672322740768286E-4 60-61 3.5642533899019947E-4 62-63 4.4553167373774933E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 5.940422316503324E-5 92-93 1.4851055791258313E-4 94-95 2.0791478107761636E-4 96-97 0.0024058710381838467 98-99 0.00843539968943472 100-101 0.018058883842170106 102-103 0.009712590487482936 104-105 0.0036830618362320615 106-107 0.010514547500210886 108-109 0.012742205868899632 110-111 0.005851315981755775 112-113 0.0038909766173096778 114-115 0.003118721716164245 116-117 0.0 118-119 0.0013068929096307315 120-121 4.1582956215523273E-4 122-123 3.8612745057271607E-4 124-125 0.033058450191341 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1683382.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.05160455217353 #Duplication Level Percentage of deduplicated Percentage of total 1 79.14978839856562 44.36472639704612 2 11.369885328677256 12.74600632493154 3 3.8582195861301147 6.487781955516474 4 1.7869319302206446 4.006416076575189 5 0.9934673225041322 2.784271874825413 6 0.6041464222060253 2.031802580946156 7 0.37769884852929875 1.4819438548302877 8 0.2706159925462372 1.21347684797565 9 0.2057729429942295 1.0380513265424547 >10 1.209707504731436 12.681769342473476 >50 0.11824745075409736 4.566685073402238 >100 0.05359556141764273 5.12801461320501 >500 0.0012016942020159351 0.4329700773755493 >1k 7.210165212095612E-4 1.036083654354496 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3190 0.18949947189645608 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2781 0.16520314462195748 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1720 0.1021752638438572 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.910633474754987E-5 0.0 16-17 0.0 0.0 0.0 1.188084463300665E-4 0.0 18-19 0.0 0.0 0.0 1.188084463300665E-4 0.0 20-21 0.0 0.0 0.0 1.4851055791258313E-4 0.0 22-23 0.0 0.0 0.0 3.8612745057271607E-4 0.0 24-25 0.0 0.0 0.0 6.534464548153657E-4 0.0 26-27 0.0 0.0 0.0 0.001514807690708348 0.0 28-29 0.0 0.0 0.0 0.005019656857445309 0.0 30-31 0.0 0.0 0.0 0.017880671172675007 0.0 32-33 0.0 0.0 0.0 0.044671975820105 0.0 34-35 0.0 0.0 0.0 0.08393816733219198 0.0 36-37 0.0 0.0 0.0 0.15213421552565015 0.0 38-39 0.0 0.0 0.0 0.2511907576533431 0.0 40-41 0.0 0.0 0.0 0.3611479747318196 0.0 42-43 0.0 0.0 0.0 0.46935276722692765 0.0 44-45 0.0 0.0 0.0 0.6029528651250875 0.0 46-47 0.0 0.0 0.0 0.7643541394644828 0.0 48-49 0.0 0.0 0.0 0.9303295389875856 0.0 50-51 0.0 0.0 0.0 1.1125816956579078 0.0 52-53 0.0 0.0 0.0 1.282774795025728 0.0 54-55 0.0 0.0 0.0 1.471175288793631 0.0 56-57 0.0 0.0 0.0 1.6915649567359043 0.0 58-59 0.0 0.0 0.0 1.9357757181673558 0.0 60-61 0.0 0.0 0.0 2.202025446393035 0.0 62-63 0.0 0.0 0.0 2.4609387530578326 0.0 64-65 0.0 0.0 0.0 2.7302180966649283 0.0 66-67 0.0 0.0 0.0 3.0407536732601397 0.0 68-69 0.0 0.0 0.0 3.382060637454838 0.0 70-71 0.0 0.0 0.0 3.7675346415727384 0.0 72-73 0.0 0.0 0.0 4.162780640401287 0.0 74-75 0.0 0.0 0.0 4.542106307421607 0.0 76-77 0.0 0.0 0.0 4.956242849216636 0.0 78-79 0.0 0.0 0.0 5.419061151895411 0.0 80-81 0.0 0.0 0.0 5.9146408836497 0.0 82-83 0.0 0.0 0.0 6.436239665150275 0.0 84-85 0.0 0.0 0.0 6.957095893861286 0.0 86-87 0.0 0.0 0.0 7.483773736442471 0.0 88-89 0.0 0.0 0.0 8.060648147598108 0.0 90-91 0.0 0.0 0.0 8.681065854333717 0.0 92-93 0.0 0.0 0.0 9.323136400412977 0.0 94-95 0.0 0.0 0.0 9.978632300927536 0.0 96-97 0.0 0.0 0.0 10.6443160257149 0.0 98-99 0.0 0.0 0.0 11.33156348351117 0.0 100-101 0.0 0.0 0.0 12.09398698572279 0.0 102-103 0.0 0.0 0.0 12.884924515053623 0.0 104-105 0.0 0.0 0.0 13.67669370350877 0.0 106-107 0.0 0.0 0.0 14.464898638574013 0.0 108-109 0.0 0.0 0.0 15.27909292127396 0.0 110-111 0.0 0.0 0.0 16.14268181553563 0.0 112-113 0.0 0.0 0.0 17.04446762529242 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2840 0.0 74.473656 1 GTATCAA 5570 0.0 55.62126 1 ATCAACG 6875 0.0 44.95394 3 TCAACGC 7015 0.0 43.9719 4 CAACGCA 7145 0.0 43.305065 5 TATCAAC 7340 0.0 42.270813 2 AACGCAG 7395 0.0 42.00199 6 GTACATG 9025 0.0 35.12021 1 ACGCAGA 8860 0.0 35.05697 7 CGCAGAG 8905 0.0 34.808857 8 TGGTATC 1280 0.0 32.567696 2 TACATGG 9610 0.0 32.347897 2 ACATGGG 9535 0.0 31.476248 3 GTGGTAT 1500 0.0 31.378235 1 GCAGAGT 10000 0.0 30.8188 9 TAAGGTG 1070 0.0 28.91726 5 CATGGGA 7235 0.0 28.47808 4 AGGTAAG 930 0.0 28.17531 2 GTAAGGT 1090 0.0 26.769651 4 GAGTACT 5585 0.0 26.738466 12-13 >>END_MODULE