FastQCFastQC Report
Thu 26 May 2016
SRR1364443_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364443_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041303
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24900.047.3191031
ATCAACG26100.042.8414843
CAACGCA26850.041.8714375
TCAACGC27750.040.7228134
AACGCAG28550.039.378226
TATCAAC29000.039.1726152
GTACATG69700.038.675511
TACATGG70300.037.0566332
GGTATCA16550.036.675231
ACATGGG68150.035.7820473
ACGCAGA35550.031.624427
CATGGGG47500.030.8026924
CGCAGAG36650.030.512958
AGGTAAG10550.023.1141832
GCAGAGT47400.023.0914129
TAAGGTG12400.023.0260735
GTAAGGT10850.023.0232544
CATGGGA31500.022.8466224
ATGGGGG27850.021.7858925
ATGGGGA30000.021.612535
Produced by FastQC (version 0.11.5)