FastQCFastQC Report
Thu 26 May 2016
SRR1364443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364443_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041303
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24880.12188293457659152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24490.1199723901841128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27750.061.4158361
GTATCAA47500.054.1960261
ATCAACG59350.043.7552383
CAACGCA61300.042.4189075
TCAACGC62250.041.9082034
AACGCAG63400.041.0098466
TATCAAC62650.040.8800742
GTACATG71600.035.9540671
ACGCAGA75550.034.414627
CGCAGAG77400.033.360628
TACATGG76300.032.942172
ACATGGG75550.032.7173353
CATGGGG49800.029.1826534
GCAGAGT90350.028.1180389
GTGGTAT17400.027.7404231
TAAGGTG12650.026.8116055
TATACCG1807.2917464E-726.4457575
TGGTATC16300.025.9438842
GTAAGGT12050.024.714244
GAGTACT54850.024.29722612-13
Produced by FastQC (version 0.11.5)