FastQCFastQC Report
Thu 26 May 2016
SRR1364442_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364442_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698585
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18250.043.705381
ATCAACG18050.041.5147673
GGTATCA12250.038.8728941
CAACGCA19450.038.5310945
TCAACGC19700.038.039884
AACGCAG21650.034.6156966
ACCGTAC700.002783823834.013781
TATCAAC22400.033.983742
GTACATG50750.033.6619151
TATACCG1251.282102E-633.307995
ACATGGG50700.031.671243
TACATGG53700.031.230872
ACGCAGA24800.029.9791077
CGCAGAG25900.028.476228
CATGGGG35300.026.620564
GAGTACA38650.023.7172411
CGTACAC2607.734943E-720.5863063
ATGGGGA21800.020.1899685
GTATTAG6850.019.98621
TAAGGTG10450.019.9210455
Produced by FastQC (version 0.11.5)