##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364442_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1698585 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.578607487997363 31.0 31.0 34.0 27.0 34.0 2 30.593531674894102 31.0 31.0 34.0 26.0 34.0 3 30.55927551461952 31.0 31.0 34.0 26.0 34.0 4 34.13318556327767 37.0 35.0 37.0 30.0 37.0 5 34.28307090902133 37.0 35.0 37.0 30.0 37.0 6 34.378623383581036 37.0 35.0 37.0 30.0 37.0 7 34.266950432271564 37.0 35.0 37.0 30.0 37.0 8 34.09157210266192 37.0 35.0 37.0 30.0 37.0 9 35.19250081685638 39.0 35.0 39.0 28.0 39.0 10-11 35.409968591504104 39.0 35.0 39.0 29.0 39.0 12-13 35.449313987819274 39.0 35.0 39.0 29.5 39.0 14-15 36.589201011430106 40.0 36.0 41.0 29.5 41.0 16-17 36.72887992063983 40.0 36.0 41.0 30.0 41.0 18-19 36.71135209600933 40.0 36.5 41.0 30.0 41.0 20-21 36.676924911028884 40.0 36.0 41.0 29.5 41.0 22-23 36.593166959557514 40.0 36.0 41.0 29.0 41.0 24-25 36.6415157322124 40.0 36.0 41.0 29.5 41.0 26-27 36.64333430473012 40.0 36.0 41.0 30.0 41.0 28-29 36.590938339853466 40.0 36.0 41.0 29.5 41.0 30-31 36.36321055466756 40.0 36.0 41.0 29.0 41.0 32-33 36.43655572137986 40.0 36.0 41.0 30.0 41.0 34-35 36.38703626842342 40.0 36.0 41.0 29.0 41.0 36-37 36.260898630330544 40.0 35.5 41.0 29.0 41.0 38-39 36.11901435606696 40.0 35.0 41.0 28.5 41.0 40-41 36.0827903814057 40.0 35.0 41.0 28.0 41.0 42-43 35.94003508802915 39.5 35.0 41.0 28.0 41.0 44-45 35.91582552536376 39.0 35.0 41.0 28.0 41.0 46-47 35.77176090687249 39.0 35.0 41.0 28.0 41.0 48-49 35.605879894147186 39.0 35.0 41.0 27.0 41.0 50-51 35.47144505573757 39.0 34.5 41.0 27.5 41.0 52-53 35.336693188742395 39.0 34.0 40.0 27.0 41.0 54-55 35.20682038284808 38.5 34.0 40.0 27.0 41.0 56-57 35.169008027269754 38.5 34.0 40.0 27.5 41.0 58-59 35.15284074685694 38.0 34.0 40.0 27.0 41.0 60-61 34.96391025471201 38.0 34.0 40.0 27.0 41.0 62-63 34.708690468831406 37.5 34.0 40.0 26.0 41.0 64-65 34.41122081026266 37.0 34.0 40.0 26.0 41.0 66-67 34.105129269362436 37.0 33.0 40.0 26.0 41.0 68-69 33.81114869141079 36.0 33.0 39.0 26.0 41.0 70-71 33.46433590311936 36.0 33.0 39.0 26.0 41.0 72-73 33.13944989505971 35.0 33.0 38.5 25.5 40.0 74-75 32.78781161967167 35.0 32.5 37.5 25.0 40.0 76-77 32.440229661747864 35.0 32.0 37.0 24.5 39.0 78-79 32.11833761631004 35.0 32.0 36.5 24.0 39.0 80-81 31.798746603790804 35.0 32.0 36.0 23.5 38.5 82-83 31.501827403397535 35.0 32.0 36.0 23.0 37.0 84-85 31.161928016554956 35.0 31.5 35.0 21.0 37.0 86-87 30.968214425536548 35.0 31.0 35.0 20.0 36.5 88-89 30.82043465590477 35.0 31.0 35.0 20.0 36.0 90-91 30.684975141073302 35.0 31.0 35.0 20.0 36.0 92-93 30.459980807554523 34.0 31.0 35.0 18.5 36.0 94-95 30.37923712972857 34.0 31.0 35.0 18.5 35.0 96-97 30.302283371158936 34.0 31.0 35.0 18.0 35.0 98-99 30.14850920030496 34.0 31.0 35.0 17.0 35.0 100-101 30.01915594450675 34.0 31.0 35.0 13.5 35.0 102-103 28.968405172540674 33.5 29.0 34.5 10.0 35.0 104-105 29.92159532787585 34.0 30.5 35.0 15.0 35.0 106-107 30.166905100421822 34.5 31.0 35.0 14.5 35.0 108-109 30.159157769555247 35.0 31.0 35.0 10.0 35.0 110-111 30.04804557911438 35.0 31.0 35.0 7.0 35.0 112-113 29.931930400892508 35.0 31.0 35.0 2.0 35.0 114-115 29.827751628561423 35.0 31.0 35.0 2.0 35.0 116-117 29.707508308386096 35.0 31.0 35.0 2.0 35.0 118-119 29.45005548736154 34.0 31.0 35.0 2.0 35.0 120-121 29.32654886272986 34.0 31.0 35.0 2.0 35.0 122-123 29.095956928855486 34.0 31.0 35.0 2.0 35.0 124-125 27.934141064474254 33.5 27.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5416201684497075 1101 2 0.528107567209581 1101 3 0.7247589293094912 1101 4 0.6175508150766262 1101 5 0.5212377809889119 1101 6 0.5164517540833913 1101 7 0.6202895425301023 1101 8 0.6901113993484245 1101 9 1.0837627309659368 1101 10-11 0.9179999172071049 1101 12-13 0.776971918825879 1101 14-15 0.9323523434955376 1101 16-17 0.7181286190630871 1101 18-19 0.7539518646960204 1101 20-21 0.7945434318329134 1101 22-23 0.88529775715417 1101 24-25 0.8783286674004813 1101 26-27 0.8394496311473034 1101 28-29 0.8456808922721635 1101 30-31 0.9284853800451955 1101 32-33 0.8417607759080354 1101 34-35 0.8097309246172273 1101 36-37 0.754432249210673 1101 38-39 0.8956316496956873 1101 40-41 0.7819421674437805 1101 42-43 0.7196867360041637 1101 44-45 0.5658812345434754 1101 46-47 0.6316763262776703 1101 48-49 0.536555518864148 1101 50-51 0.5884913134204481 1101 52-53 0.5092256710166296 1101 54-55 0.5092197691198805 1101 56-57 0.5349462079134852 1101 58-59 0.6366832922612673 1101 60-61 0.5905385492330524 1101 62-63 0.584323604330784 1101 64-65 0.5884244598280333 1101 66-67 0.6333118510232723 1101 68-69 0.5696749674079058 1101 70-71 0.4216508913415993 1101 72-73 0.3976124322455945 1101 74-75 0.4050237510706509 1101 76-77 0.5820305210509034 1101 78-79 0.39819132022925885 1101 80-81 0.41787640164194784 1101 82-83 0.4812173027179121 1101 84-85 0.5564802663650958 1101 86-87 0.6417269870519036 1101 88-89 0.5878480942933209 1101 90-91 0.5733598711195143 1101 92-93 0.557742655494252 1101 94-95 0.4988085590097988 1101 96-97 0.4701677998049476 1101 98-99 0.5015858450986208 1101 100-101 0.49983075226356277 1101 102-103 0.4996830337586715 1101 104-105 0.5474494143067865 1101 106-107 0.524888795244653 1101 108-109 0.5521072301992653 1101 110-111 0.5057605503724893 1101 112-113 0.5060216286329187 1101 114-115 0.5596403580973472 1101 116-117 0.5867220780002924 1101 118-119 0.4537323713576029 1101 120-121 0.4539582322908764 1101 122-123 0.42863903094946565 1101 124-125 0.08621640632262739 1102 1 0.25147056151867986 1102 2 0.22420645063288447 1102 3 0.4238101391017217 1102 4 0.3832331235769857 1102 5 0.3420524300198551 1102 6 0.23439148619711148 1102 7 0.43071750663218467 1102 8 0.3851184530408318 1102 9 0.8028626513561079 1102 10-11 0.49530316378327655 1102 12-13 0.528831571984 1102 14-15 0.6433100423180562 1102 16-17 0.6071636296403256 1102 18-19 0.4388861546773768 1102 20-21 0.5239837557761362 1102 22-23 0.5743817482429137 1102 24-25 0.5926674737693318 1102 26-27 0.541616729064458 1102 28-29 0.4666786717929696 1102 30-31 0.5170889836554053 1102 32-33 0.3967227334972776 1102 34-35 0.24689467868665815 1102 36-37 0.34354186741938975 1102 38-39 0.4161952497676822 1102 40-41 0.3192481102218139 1102 42-43 0.37510421675118266 1102 44-45 0.21266470826365236 1102 46-47 0.3001783074286095 1102 48-49 0.3108124974135009 1102 50-51 0.13429124556065375 1102 52-53 0.203326387211753 1102 54-55 0.0593218155194819 1102 56-57 -0.015779060118759958 1102 58-59 0.11559482764847928 1102 60-61 0.04881101143305244 1102 62-63 0.08945959016148919 1102 64-65 0.1380917840178526 1102 66-67 0.07640812934054253 1102 68-69 0.08725704937155854 1102 70-71 0.22388507158255067 1102 72-73 0.20034243198008284 1102 74-75 0.190155532704118 1102 76-77 0.2844060685243761 1102 78-79 0.17289548982181913 1102 80-81 0.33278042756420945 1102 82-83 0.32030466039168104 1102 84-85 0.3600122321265573 1102 86-87 0.3617444668894336 1102 88-89 0.29724264148176616 1102 90-91 0.2923146396194838 1102 92-93 0.3770003873078345 1102 94-95 0.29831683222746364 1102 96-97 0.24876151196295737 1102 98-99 0.3661281795018816 1102 100-101 0.23330166328200974 1102 102-103 0.08337267011907912 1102 104-105 0.1669942813501457 1102 106-107 0.19173843592954753 1102 108-109 0.1638161689005777 1102 110-111 0.25150607439135086 1102 112-113 0.2935874732416224 1102 114-115 0.12627710445355333 1102 116-117 0.012188966132999468 1102 118-119 -0.00944246941173077 1102 120-121 -0.019018431773961453 1102 122-123 0.04565837245717219 1102 124-125 -0.20115631806026357 1103 1 0.502908176009619 1103 2 0.5177768806367418 1103 3 0.6670999156636164 1103 4 0.5802632889259982 1103 5 0.5729163243989532 1103 6 0.39751320211496477 1103 7 0.46449896151069936 1103 8 0.6689696570034229 1103 9 1.1600778400853784 1103 10-11 0.8046629485403116 1103 12-13 0.6660070052418092 1103 14-15 0.8038141338625948 1103 16-17 0.8135113988747946 1103 18-19 0.775299394650375 1103 20-21 0.7636707586444302 1103 22-23 0.7289103920676396 1103 24-25 0.6261786697778149 1103 26-27 0.7708800853361595 1103 28-29 0.7805014187318449 1103 30-31 0.9673763408430247 1103 32-33 0.7562586987161453 1103 34-35 0.5978171592834514 1103 36-37 0.5507346098991519 1103 38-39 0.7341209903432713 1103 40-41 0.6847868654040354 1103 42-43 0.8106842338645635 1103 44-45 0.681588252420994 1103 46-47 0.5762332811559503 1103 48-49 0.5175259597399489 1103 50-51 0.5345024512104786 1103 52-53 0.5055138420179617 1103 54-55 0.31805882983687894 1103 56-57 0.3379669025692422 1103 58-59 0.5285552017325443 1103 60-61 0.6041749871609738 1103 62-63 0.5668205138020639 1103 64-65 0.5571780258227008 1103 66-67 0.46032418639318706 1103 68-69 0.45989559847009076 1103 70-71 0.5531757700977806 1103 72-73 0.4676649860921174 1103 74-75 0.5103451809156496 1103 76-77 0.48805185164238196 1103 78-79 0.36481182707596815 1103 80-81 0.32451576368550406 1103 82-83 0.3300561999934928 1103 84-85 0.46366916304634387 1103 86-87 0.40800701569872544 1103 88-89 0.4302986606921415 1103 90-91 0.4208938372187916 1103 92-93 0.30270397931034054 1103 94-95 0.3357673450263441 1103 96-97 0.331090664368471 1103 98-99 0.4379445782195823 1103 100-101 0.3129971475184128 1103 102-103 0.33272880924364756 1103 104-105 0.3283262478223783 1103 106-107 0.312461898135485 1103 108-109 0.34942849957450406 1103 110-111 0.4220534314584512 1103 112-113 0.46616614056711825 1103 114-115 0.40080731499636 1103 116-117 0.3416348299972505 1103 118-119 0.23059486065961465 1103 120-121 0.11501437590537122 1103 122-123 0.27347680790838425 1103 124-125 0.16285631812369417 1104 1 0.42091879512033614 1104 2 0.47249444051110245 1104 3 0.5626996715038217 1104 4 0.3605596547485348 1104 5 0.4136224553863883 1104 6 0.2829597752892852 1104 7 0.4392492805134509 1104 8 0.5300818416814792 1104 9 0.9040233826354367 1104 10-11 0.7300738179454953 1104 12-13 0.6976728590649373 1104 14-15 0.7448015393887104 1104 16-17 0.654545734612924 1104 18-19 0.5830845953767252 1104 20-21 0.6228052029661839 1104 22-23 0.7545139667388199 1104 24-25 0.6848767091378463 1104 26-27 0.645048607739966 1104 28-29 0.6794098312023991 1104 30-31 0.7536220312385851 1104 32-33 0.7316676510375046 1104 34-35 0.6973370448105456 1104 36-37 0.6927586209906238 1104 38-39 0.8059221442743691 1104 40-41 0.7234115801419634 1104 42-43 0.763385174922675 1104 44-45 0.6219273768811391 1104 46-47 0.6095468301842217 1104 48-49 0.5501026584295872 1104 50-51 0.6229573123215957 1104 52-53 0.45614425586268226 1104 54-55 0.47002330397312164 1104 56-57 0.533490128423189 1104 58-59 0.5861450132755408 1104 60-61 0.742183627841186 1104 62-63 0.7200829303705731 1104 64-65 0.5351435244485359 1104 66-67 0.6250209344055548 1104 68-69 0.653198151105542 1104 70-71 0.712906887588943 1104 72-73 0.6435364923985532 1104 74-75 0.6164863672377905 1104 76-77 0.6021991634307611 1104 78-79 0.41677798824596834 1104 80-81 0.5702166648682621 1104 82-83 0.5118174479077062 1104 84-85 0.498174064646264 1104 86-87 0.5220266497044186 1104 88-89 0.5311444013919839 1104 90-91 0.49531027690273177 1104 92-93 0.5005783934495263 1104 94-95 0.4067530481820185 1104 96-97 0.4093257049799739 1104 98-99 0.5019695033766283 1104 100-101 0.44094868977400736 1104 102-103 0.39200289395114396 1104 104-105 0.40656849666906325 1104 106-107 0.3373214142328571 1104 108-109 0.26788641268306534 1104 110-111 0.3179675890458107 1104 112-113 0.27842244087139534 1104 114-115 0.31211281840723615 1104 116-117 0.2512110471154294 1104 118-119 0.1987263327346085 1104 120-121 0.032851307221623216 1104 122-123 0.022718716280980544 1104 124-125 -0.2168298015759511 1105 1 0.254959515295333 1105 2 0.2551756901605522 1105 3 0.2878759573302858 1105 4 0.254139777021237 1105 5 0.1963858184465721 1105 6 0.1498323985435377 1105 7 0.2235674138916579 1105 8 0.3534406391462781 1105 9 0.6967839412758536 1105 10-11 0.3486220259212658 1105 12-13 0.41466809155133433 1105 14-15 0.026680949338789617 1105 16-17 0.21691244054146352 1105 18-19 0.2335300494122805 1105 20-21 0.4516413687634824 1105 22-23 0.5789821926628775 1105 24-25 0.5469331340635293 1105 26-27 0.41448536807425285 1105 28-29 0.38770193956518284 1105 30-31 0.5961996295334941 1105 32-33 0.46070326716852605 1105 34-35 0.4203368765453561 1105 36-37 0.4815247884705798 1105 38-39 0.5593066302242278 1105 40-41 0.4834970588564147 1105 42-43 0.45919985886456516 1105 44-45 0.5051239667067122 1105 46-47 0.5719483109178611 1105 48-49 0.30767290914472056 1105 50-51 0.3428938202581051 1105 52-53 0.25950305332748513 1105 54-55 0.29929022864640586 1105 56-57 0.3484475573311485 1105 58-59 0.4853528207801645 1105 60-61 0.3946359246609745 1105 62-63 0.5387074185639662 1105 64-65 0.42445778772746934 1105 66-67 0.32226205841699596 1105 68-69 0.3487687383510405 1105 70-71 0.3782516629549164 1105 72-73 0.31386550692545256 1105 74-75 0.45102784460612355 1105 76-77 0.3161790837852365 1105 78-79 0.38336837469501006 1105 80-81 0.35784872499502995 1105 82-83 0.38977829820777643 1105 84-85 0.4383663356653891 1105 86-87 0.40151557224634615 1105 88-89 0.3221956100969052 1105 90-91 0.2743439860283168 1105 92-93 0.2933970596674875 1105 94-95 0.32243289562158495 1105 96-97 0.29649133401132843 1105 98-99 0.2536179413148183 1105 100-101 0.07091604632793747 1105 102-103 0.07030767829126461 1105 104-105 0.05731099484619051 1105 106-107 0.12500058861167673 1105 108-109 0.12407172874117478 1105 110-111 0.10092322312511826 1105 112-113 0.0505150989004477 1105 114-115 -0.13080243202745123 1105 116-117 -0.15168064619322763 1105 118-119 -0.3098448714832429 1105 120-121 -0.33818688897558147 1105 122-123 -0.27798822185351924 1105 124-125 -0.35569502116202045 1106 1 0.3806371877373067 1106 2 0.4438090125970753 1106 3 0.5081566590846691 1106 4 0.3205754313745146 1106 5 0.31365246331962027 1106 6 0.20117423138650992 1106 7 0.09866777348629086 1106 8 0.43025964317809695 1106 9 0.8595316801883541 1106 10-11 0.34321742746861617 1106 12-13 0.420246820983607 1106 14-15 0.6900552546673282 1106 16-17 0.6348575206329983 1106 18-19 0.4756575420569362 1106 20-21 0.6176912761406186 1106 22-23 0.7168625457194295 1106 24-25 0.6506977630272459 1106 26-27 0.6142190501054188 1106 28-29 0.5068781409381771 1106 30-31 0.6076186117717057 1106 32-33 0.6213725490689299 1106 34-35 0.5404597925309744 1106 36-37 0.5847931768327825 1106 38-39 0.7483230844031539 1106 40-41 0.7854767907949523 1106 42-43 0.7686048931895044 1106 44-45 0.5000998847627187 1106 46-47 0.5933465892223069 1106 48-49 0.5696201685976945 1106 50-51 0.5846913575796648 1106 52-53 0.5784807148726543 1106 54-55 0.3595768146767 1106 56-57 0.38664217430837766 1106 58-59 0.43862861897129335 1106 60-61 0.3965825303055155 1106 62-63 0.4364214863421054 1106 64-65 0.4687269388661903 1106 66-67 0.32486029313422193 1106 68-69 0.3770117371524009 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-1.5569639495070007 2216 86-87 -1.566793782220902 2216 88-89 -1.9676451404280577 2216 90-91 -1.6604811850073808 2216 92-93 -1.5703676223077245 2216 94-95 -1.5003949171535957 2216 96-97 -1.4321408545682281 2216 98-99 -1.456354963921246 2216 100-101 -1.6344240606963787 2216 102-103 -1.8281809080394282 2216 104-105 -1.7158202115481735 2216 106-107 -1.4234756018645136 2216 108-109 -1.4432649237007418 2216 110-111 -1.398513002574262 2216 112-113 -1.7971923453859446 2216 114-115 -1.6479116239261131 2216 116-117 -1.5102099424067355 2216 118-119 -1.3766892120644236 2216 120-121 -1.2644616976188097 2216 122-123 -1.2245378391399804 2216 124-125 -1.1631433040313581 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29455.0 3 5820.0 4 3596.0 5 3073.0 6 3356.0 7 4033.0 8 4519.0 9 5237.0 10 5828.0 11 6363.0 12 6913.0 13 6845.0 14 7221.0 15 6829.0 16 6486.0 17 6098.0 18 5729.0 19 5758.0 20 5631.0 21 6005.0 22 7007.0 23 9018.0 24 12887.0 25 17958.0 26 21586.0 27 24180.0 28 28675.0 29 34091.0 30 40810.0 31 49131.0 32 62219.0 33 81214.0 34 109574.0 35 158398.0 36 277616.0 37 451213.0 38 177801.0 39 412.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.07217892000311 18.489130972779705 12.532790922953998 24.905899184263188 2 15.572586765070373 19.910442893214984 39.504680671690075 25.01228967002457 3 18.538724879826443 24.200319677849507 29.820585958312364 27.44036948401169 4 12.346692947875603 16.241834221148366 37.663963187467914 33.74750964350812 5 13.176569910654152 38.46440060097682 35.02779988790557 13.331229600463448 6 32.532016467882855 36.98503278328532 17.30988078858858 13.17306996024324 7 28.442497726048444 32.5081170503684 21.372671959307304 17.676713264275854 8 26.097999078046374 35.629563037548714 20.150927222770722 18.12151066163419 9 26.11557889531256 15.871845894806249 19.933473842856976 38.07910136702422 10-11 24.544618469852114 26.55755880054945 28.431074692756937 20.466748036841494 12-13 25.221755385992576 23.949552250317108 28.052052648450925 22.776639715239387 14-15 22.138008160655584 25.712099771830164 25.13394785034343 27.01594421717082 16-17 22.699177734753885 28.80212676832067 26.10330816563289 22.39538733129256 18-19 22.798019596188162 27.757831836430917 27.223045084304353 22.22110348307657 20-21 22.988750415860707 27.15777976016982 27.32627326979865 22.527196554170825 22-23 22.948621855656505 27.019171494229592 27.113612932589188 22.918593717524715 24-25 22.82393914236424 27.228905021814775 27.070137012111473 22.877018823709513 26-27 22.603465426837115 27.547985448704683 26.826220240101673 23.022328884356533 28-29 22.678849992772786 27.670043389778215 26.764330711419852 22.886775906029143 30-31 22.78645998176379 27.24293759029412 26.834757380969275 23.13584504697282 32-33 22.39099065259896 27.441483809397088 26.861988511357062 23.305537026646896 34-35 23.18335464055362 27.41922807124962 26.58110809516394 22.81630919303282 36-37 23.204018188171094 26.96187071939356 26.683843795083707 23.150267297351633 38-39 22.74334629021552 27.23694610064569 26.735086461799767 23.28462114733903 40-41 23.125491223473933 27.063992252283548 26.805126680462628 23.0053898437799 42-43 22.8238663793611 27.00981431111425 26.793540334640337 23.372778974884312 44-45 22.732282291740873 27.239576372817353 26.968936793265392 23.059204542176385 46-47 23.12408525349149 27.493232494857402 26.401777272250932 22.980904979400172 48-49 22.81070772716327 27.400663080232 26.50896088580265 23.27966830680208 50-51 22.95201166208222 27.457203313990732 26.678779343607523 22.91200568031953 52-53 23.234915917256128 27.189648655300196 26.55539239987612 23.020043027567553 54-55 22.615509345667835 27.339566918702758 26.835808750884638 23.209114984744772 56-57 22.605756059447664 27.61493675082129 26.754598367935966 23.02470882179508 58-59 22.809323077982878 27.14935936442498 26.66870972936485 23.372607828227295 60-61 22.446269955569896 27.292101144725795 27.08037220957632 23.18125669012799 62-63 22.566001851805513 27.323728870943288 27.171286121615633 22.93898315563557 64-65 22.43629203027378 27.274456138553553 26.875029254898703 23.41422257627396 66-67 22.351134925074337 27.445991697824358 26.805517119557216 23.397356257544086 68-69 22.671706845310382 26.966368878074487 27.197416319639178 23.164507956975953 70-71 22.57886370326759 26.911775095672652 26.88233735649102 23.627023844568736 72-73 22.53332626769354 27.15602110271085 26.70043453684731 23.610218092748298 74-75 22.526813674878507 27.399040416218067 26.80996537502344 23.264180533879987 76-77 22.71455553409856 27.05011751628507 26.9335432455219 23.301783704094465 78-79 22.7399099997115 27.083839925955537 26.92699001848168 23.249260055851284 80-81 22.838884649445106 27.268426271362085 26.68673925536525 23.205949823827556 82-83 22.687728742260926 27.310077864005468 26.83930785326225 23.162885540471354 84-85 22.630522035147777 27.25947371659502 26.90418634392866 23.20581790432854 86-87 22.72886523636689 27.24705174375196 26.758737602249788 23.265345417631362 88-89 22.622666445660634 27.31652254659012 26.691925340392963 23.368885667356277 90-91 22.59129297627217 27.37371747937501 26.83752516023504 23.19746438411778 92-93 22.73536314381972 26.857611023905775 27.0900920739182 23.316933758356306 94-95 22.385420760490863 26.847538259316995 27.23998604603924 23.527054934152908 96-97 22.620161049823704 26.56618495382616 27.25703352854435 23.556620467805786 98-99 22.556203722893155 26.72317458597886 26.956444175443224 23.764177515684757 100-101 22.284447215669513 26.711832532435555 27.316723057760317 23.68699719413462 102-103 22.176989509105013 26.42713413242933 27.444752418116703 23.95112394034895 104-105 22.06742511134087 26.757048448293325 27.111502200910508 24.064024239455296 106-107 22.232132974426726 26.722704610913684 26.994062968798204 24.051099445861386 108-109 22.295835176846243 26.583058756620087 26.881347240706415 24.239758825827256 110-111 22.26706667526267 26.76789933960901 27.066168922676287 23.898865062452035 112-113 22.284989247438446 26.7966492633649 27.255189711690043 23.663171777506612 114-115 22.16487545945532 27.01218982625707 26.97833328671926 23.84460142756835 116-117 22.04281353848473 26.913049314997515 27.189723431865982 23.854413714651777 118-119 21.810220287346198 27.14047135019555 26.934848979661673 24.11445938279658 120-121 21.944129228982654 27.239415287259593 26.974241711338276 23.842213772419473 122-123 21.86071861086664 27.258294228894425 26.985139773402818 23.895847386836117 124-125 21.783908840940494 27.27713974553524 26.975235405424304 23.963716008099958 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 431.0 1 376.0 2 283.0 3 216.0 4 177.5 5 155.0 6 136.0 7 136.0 8 125.5 9 117.5 10 109.0 11 101.5 12 113.5 13 127.0 14 144.0 15 161.5 16 208.5 17 247.0 18 299.0 19 388.5 20 520.0 21 746.5 22 1164.5 23 1773.0 24 2615.5 25 3876.0 26 5643.0 27 7944.5 28 10654.5 29 13845.5 30 17567.0 31 21871.0 32 27960.5 33 31584.5 34 32909.5 35 36084.5 36 40532.0 37 45238.5 38 48614.5 39 51176.5 40 53394.0 41 56370.5 42 58615.0 43 60083.0 44 61645.0 45 64439.5 46 65752.5 47 64902.5 48 64203.5 49 62895.0 50 62160.5 51 60808.0 52 59570.5 53 60090.0 54 61301.5 55 58037.0 56 51936.0 57 47544.0 58 44261.5 59 38514.0 60 30572.0 61 25315.5 62 21263.0 63 15919.0 64 11332.0 65 8537.5 66 6173.5 67 4830.5 68 3828.0 69 2624.5 70 1719.0 71 1108.5 72 795.0 73 567.5 74 427.5 75 322.0 76 206.0 77 115.0 78 71.5 79 52.0 80 32.5 81 17.5 82 13.5 83 13.0 84 10.5 85 6.5 86 5.0 87 3.0 88 2.0 89 2.5 90 2.5 91 1.5 92 1.5 93 2.0 94 1.5 95 2.5 96 1.5 97 1.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08648374970931687 2 2.3549012854817393E-4 3 0.0 4 0.001942793560522435 5 0.001000833046329739 6 3.5323519282226085E-4 7 0.0 8 0.0010597055784667827 9 2.9436266068521736E-4 10-11 0.010243820591845565 12-13 0.0020899748908650433 14-15 0.004091640983524522 16-17 0.0026787002122354783 18-19 0.014394334107507132 20-21 0.017661759641113044 22-23 0.010479310720393738 24-25 0.011480143766723478 26-27 0.010567619518599304 28-29 0.00844820836166574 30-31 0.01857428388923722 32-33 0.002237156221207652 34-35 0.001265759440946435 36-37 5.004165231648696E-4 38-39 1.4718133034260868E-4 40-41 0.0011774506427408694 42-43 0.0026492639461669566 44-45 9.419605141926957E-4 46-47 0.001589558367700174 48-49 0.008713134756282435 50-51 0.005239655360196869 52-53 0.008183281967049044 54-55 0.008889752352693566 56-57 0.010803109647147478 58-59 0.009095806215173217 60-61 0.011009163509627131 62-63 0.014276589043233043 64-65 0.008919188618762088 66-67 0.013835045052205218 68-69 0.01368786372186261 70-71 0.0050041652316486955 72-73 0.013246319730834785 74-75 0.014394334107507132 76-77 0.005710635617293218 78-79 0.008595389692008348 80-81 0.014659260502123826 82-83 0.017867813503592695 84-85 0.014217716511096 86-87 0.01724965191615374 88-89 0.01710247058581113 90-91 0.012981393336218087 92-93 0.00983171286688626 94-95 0.008389335829528695 96-97 0.00980227660081774 98-99 0.007123576388582261 100-101 0.010508746986462262 102-103 0.012216050418436522 104-105 0.011597888830997567 106-107 0.005475145488745044 108-109 0.003620660726428174 110-111 0.007153012654650783 112-113 0.00897806115089913 114-115 0.014276589043233043 116-117 0.011597888830997567 118-119 0.21962398113724071 120-121 0.02661038452594365 122-123 0.027169673581245564 124-125 0.2359905450713388 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1698585.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.85279739309074 #Duplication Level Percentage of deduplicated Percentage of total 1 87.27342719507499 67.07207018023753 2 8.244657684520165 12.672500132076339 3 2.2081423895914947 5.09105759047111 4 0.8564516643814638 2.632828249587359 5 0.41640213291346445 1.6000834377424662 6 0.2552206827784553 1.1768654054459344 7 0.1522403722795302 0.819006894009321 8 0.10714833503806943 0.6587719426950204 9 0.06990464308778911 0.48351306348559603 >10 0.3816486545224495 5.336929688966506 >50 0.025500190869370918 1.3588095187062947 >100 0.009113654166999772 1.0277418274590642 >500 1.4240077592505185E-4 0.06982206911742059 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.887253213704348E-5 0.0 12-13 5.887253213704348E-5 0.0 0.0 5.887253213704348E-5 0.0 14-15 1.1774506427408697E-4 0.0 0.0 5.887253213704348E-5 0.0 16-17 1.7661759641113045E-4 0.0 0.0 5.887253213704348E-5 0.0 18-19 2.060538624796522E-4 0.0 0.0 1.471813303426087E-4 0.0 20-21 2.3549012854817393E-4 0.0 0.0 1.7661759641113045E-4 0.0 22-23 2.943626606852174E-4 0.0 0.0 2.060538624796522E-4 0.0 24-25 5.298527892333913E-4 0.0 0.0 4.121077249593044E-4 0.0 26-27 5.298527892333913E-4 0.0 0.0 7.653429177815653E-4 0.0 28-29 6.475978535074783E-4 0.0 0.0 0.0023254650194132176 0.0 30-31 7.064703856445218E-4 0.0 0.0 0.00912524248124174 0.0 32-33 7.064703856445218E-4 0.0 0.0 0.02322521392806365 0.0 34-35 7.064703856445218E-4 0.0 0.0 0.046509300388264355 0.0 36-37 8.242154499186088E-4 0.0 0.0 0.08130296688125704 0.0 38-39 9.713967802612174E-4 0.0 0.0 0.13829157798991515 2.943626606852174E-5 40-41 0.001000833046329739 0.0 0.0 0.19901859488927548 5.887253213704348E-5 42-43 0.0010597055784667825 0.0 0.0 0.25756732809956523 5.887253213704348E-5 44-45 0.001118578110603826 0.0 0.0 0.331687846060103 5.887253213704348E-5 46-47 0.0012068869088093914 0.0 0.0 0.42096804104592944 5.887253213704348E-5 48-49 0.0012951957070149566 0.0 0.0 0.51793110147564 5.887253213704348E-5 50-51 0.0012951957070149566 0.0 0.0 0.6246081297079629 5.887253213704348E-5 52-53 0.0013246319730834783 0.0 0.0 0.7256628311211979 5.887253213704348E-5 54-55 0.0014423770373575653 0.0 0.0 0.8346358881068654 5.887253213704348E-5 56-57 0.001471813303426087 0.0 0.0 0.9703959472148878 5.887253213704348E-5 58-59 0.0015306858355631306 0.0 0.0 1.1136916904364516 5.887253213704348E-5 60-61 0.0015306858355631306 0.0 0.0 1.2722354194815098 5.887253213704348E-5 62-63 0.0015306858355631306 0.0 0.0 1.4340760103262422 5.887253213704348E-5 64-65 0.0015306858355631306 0.0 0.0 1.5952395670513986 5.887253213704348E-5 66-67 0.001589558367700174 0.0 0.0 1.7834550522935266 5.887253213704348E-5 68-69 0.001589558367700174 0.0 0.0 1.9996644265668189 5.887253213704348E-5 70-71 0.0016484308998372175 0.0 0.0 2.231386713058222 5.887253213704348E-5 72-73 0.0017367396980427826 0.0 0.0 2.4727052222879635 5.887253213704348E-5 74-75 0.0018250484962483479 0.0 0.0 2.709549419075289 5.887253213704348E-5 76-77 0.0018250484962483479 0.0 0.0 2.9658215514678394 5.887253213704348E-5 78-79 0.0018250484962483479 0.0 0.0 3.266542445623857 5.887253213704348E-5 80-81 0.0019427935605224348 0.0 0.0 3.5896643382580207 5.887253213704348E-5 82-83 0.0019427935605224348 0.0 0.0 3.9276515452567873 5.887253213704348E-5 84-85 0.0019722298265909565 0.0 0.0 4.258662357197315 5.887253213704348E-5 86-87 0.002001666092659478 0.0 0.0 4.605156645089884 5.887253213704348E-5 88-89 0.002001666092659478 0.0 0.0 4.9759947250211205 5.887253213704348E-5 90-91 0.0021194111569335654 0.0 0.0 5.401878622500493 5.887253213704348E-5 92-93 0.0021782836890706088 0.0 0.0 5.835680875552298 5.887253213704348E-5 94-95 0.0021782836890706088 0.0 0.0 6.268482295557773 5.887253213704348E-5 96-97 0.002237156221207652 0.0 0.0 6.7160901573957155 5.887253213704348E-5 98-99 0.0025315188818928695 0.0 0.0 7.178268970937575 5.887253213704348E-5 100-101 0.0027670090104410434 0.0 0.0 7.68195291963605 5.887253213704348E-5 102-103 0.0030319354050577394 0.0 0.0 8.22240276465411 5.887253213704348E-5 104-105 0.003620660726428174 0.0 0.0 8.770035058592889 5.887253213704348E-5 106-107 0.003944459653181913 0.0 0.0 9.312457133437537 5.887253213704348E-5 108-109 0.0040327684513874786 0.0 0.0 9.888995840655603 5.887253213704348E-5 110-111 0.004062204717456 0.0 0.0 10.504625909212667 5.887253213704348E-5 112-113 0.004121077249593044 0.0 0.0 11.155785550914437 5.887253213704348E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1825 0.0 43.70538 1 ATCAACG 1805 0.0 41.514767 3 GGTATCA 1225 0.0 38.872894 1 CAACGCA 1945 0.0 38.531094 5 TCAACGC 1970 0.0 38.03988 4 AACGCAG 2165 0.0 34.615696 6 ACCGTAC 70 0.0027838238 34.01378 1 TATCAAC 2240 0.0 33.98374 2 GTACATG 5075 0.0 33.661915 1 TATACCG 125 1.282102E-6 33.30799 5 ACATGGG 5070 0.0 31.67124 3 TACATGG 5370 0.0 31.23087 2 ACGCAGA 2480 0.0 29.979107 7 CGCAGAG 2590 0.0 28.47622 8 CATGGGG 3530 0.0 26.62056 4 GAGTACA 3865 0.0 23.717241 1 CGTACAC 260 7.734943E-7 20.586306 3 ATGGGGA 2180 0.0 20.189968 5 GTATTAG 685 0.0 19.9862 1 TAAGGTG 1045 0.0 19.921045 5 >>END_MODULE