FastQCFastQC Report
Thu 26 May 2016
SRR1364442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364442_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698585
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20890.12298471963428383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20460.12045320075239095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.069.798081
GTATCAA42000.051.084611
ATCAACG51150.041.687463
TCAACGC52050.041.081074
CAACGCA52850.040.415155
AACGCAG55050.039.015026
TATCAAC55200.038.3074232
ACGCAGA62200.034.3388677
CGCAGAG63700.033.435888
GTACATG53300.032.4270671
TACATGG55900.030.0490632
ACATGGG54700.029.835363
GCAGAGT76300.027.5244949
TAAGGTG10750.026.5660825
TGGTATC10700.026.1642152
GTGGTAT11700.025.9789261
GTAAGGT9450.025.2113574
GAGTACT49650.025.10347712-13
CAGAGTA74750.024.99122810-11
AGAGTAC71200.024.81679210-11
Produced by FastQC (version 0.11.5)