FastQCFastQC Report
Thu 26 May 2016
SRR1364441_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364441_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095315
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23300.046.756771
ATCAACG24650.041.0143173
TCAACGC25150.039.963414
CAACGCA26400.038.3037875
GGTATCA15200.037.20741
GTACATG67900.036.4731181
AACGCAG28050.036.0497636
TACATGG68950.035.104572
TATCAAC29600.034.959162
ACATGGG67400.034.8530353
CGCAGAG33500.029.6522778
ACGCAGA34150.029.262067
CATGGGG44350.026.417184
CGCGGAA3100.025.90844782-83
CATGGGA34550.025.6479154
TAAGGTG13300.025.492945
GTGTAAG10400.025.1865481
TAAGGTA9850.022.3398024
GTAAGGT14500.022.1482564
GGTAAGG14400.021.8885423
Produced by FastQC (version 0.11.5)