FastQCFastQC Report
Thu 26 May 2016
SRR1364441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364441_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095315
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21670.10342120397171786No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.063.5379641
GTATCAA42850.054.214861
ATCAACG53800.042.937443
TCAACGC54850.042.224034
CAACGCA55750.041.5146265
TATCAAC58050.039.6951032
AACGCAG58750.039.3947336
GTACATG71000.033.05541
TACATGG69750.032.9512022
ACGCAGA70250.032.9457747
CGCAGAG70800.032.5202148
TAAGGTG13700.031.7028795
ACATGGG69400.031.3984583
GTAAGGT14700.027.9459234
GCAGAGT86400.025.8910589
AGGTAAG14500.025.0489792
GGTAAGG15550.024.5039623
CAGAGTA79000.024.24959410-11
AGAGTAC77750.024.218610-11
CATGGGG47250.024.19284
Produced by FastQC (version 0.11.5)