FastQCFastQC Report
Thu 26 May 2016
SRR1364440_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364440_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153245
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.066.1814651
GTATCAA24800.056.1589621
TCAACGC28700.046.0050664
CAACGCA29050.045.4586755
ATCAACG29050.045.2421343
CCGTTGC1002.2306267E-741.637939
AACGCAG31600.041.410256
GTACATG79700.041.073271
TATCAAC34050.039.2972642
TACATGG85100.037.7364582
ACATGGG82400.036.663463
CATGGGA60800.034.1394424
ACGCAGA38500.033.6796887
CGCAGAG39800.032.430158
CATGGGG23050.031.4791874
ATGGGAT26050.030.3706325
ATGGGGA13200.028.8413755
TCCGTTG1704.4375702E-727.9906738
GAGTACA55950.027.0201971
GCAGAGT46500.026.7352629
Produced by FastQC (version 0.11.5)