##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364440_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1153245 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.59883285858599 31.0 31.0 34.0 26.0 34.0 2 30.637932095955325 31.0 31.0 34.0 26.0 34.0 3 30.553129647212863 31.0 31.0 34.0 26.0 34.0 4 34.083500036852534 37.0 35.0 37.0 30.0 37.0 5 34.33332639638585 37.0 35.0 37.0 30.0 37.0 6 34.36513490195058 37.0 35.0 37.0 30.0 37.0 7 34.28122818655186 37.0 35.0 37.0 30.0 37.0 8 34.11663003091277 37.0 35.0 37.0 30.0 37.0 9 35.13617488044605 39.0 35.0 39.0 27.0 39.0 10-11 35.42107184509796 39.0 35.0 39.0 29.0 39.0 12-13 35.49254971840328 39.0 35.0 39.0 30.0 39.0 14-15 36.622219476347176 40.0 36.0 41.0 29.5 41.0 16-17 36.78320651726216 40.0 36.5 41.0 30.0 41.0 18-19 36.79514587099879 40.0 36.5 41.0 30.0 41.0 20-21 36.758754210943906 40.0 37.0 41.0 29.5 41.0 22-23 36.67431811974039 40.0 36.0 41.0 29.0 41.0 24-25 36.74377040438068 40.0 36.0 41.0 29.5 41.0 26-27 36.75270996189015 40.0 36.0 41.0 30.0 41.0 28-29 36.71155955586194 40.0 36.0 41.0 30.0 41.0 30-31 36.48502789953566 40.0 36.0 41.0 29.5 41.0 32-33 36.554856080017686 40.0 36.0 41.0 30.0 41.0 34-35 36.50380621637206 40.0 36.0 41.0 30.0 41.0 36-37 36.37999037498537 40.0 36.0 41.0 29.0 41.0 38-39 36.235547953817274 40.0 35.5 41.0 28.5 41.0 40-41 36.21584442161033 40.0 35.0 41.0 29.0 41.0 42-43 36.087405538285445 40.0 35.0 41.0 28.0 41.0 44-45 36.06806402802527 40.0 35.0 41.0 28.0 41.0 46-47 35.93669038235588 39.0 35.0 41.0 28.0 41.0 48-49 35.78254187098145 39.0 35.0 41.0 27.5 41.0 50-51 35.66966256086087 39.0 35.0 41.0 27.5 41.0 52-53 35.55001625847066 39.0 35.0 41.0 28.0 41.0 54-55 35.4365733213671 39.0 34.0 40.5 28.0 41.0 56-57 35.40071754050527 39.0 34.5 40.5 28.0 41.0 58-59 35.38553733161644 39.0 34.5 41.0 28.0 41.0 60-61 35.20475787885488 38.0 34.0 40.5 28.0 41.0 62-63 34.95281618389848 38.0 34.0 40.0 27.5 41.0 64-65 34.65594127873956 37.5 34.0 40.0 26.5 41.0 66-67 34.355428811744254 37.0 34.0 40.0 26.0 41.0 68-69 34.04770061868901 36.5 33.5 39.5 26.0 41.0 70-71 33.69227570897771 36.0 33.0 39.0 26.0 41.0 72-73 33.36126321813665 35.5 33.0 39.0 26.0 40.5 74-75 32.99350918495203 35.0 33.0 37.5 26.0 40.0 76-77 32.63892971571522 35.0 32.5 37.0 25.0 39.0 78-79 32.30610798225875 35.0 32.0 37.0 25.0 39.0 80-81 31.965605313701772 35.0 32.0 36.0 24.5 38.5 82-83 31.651203343608685 35.0 32.0 36.0 24.0 37.0 84-85 31.27486180299936 35.0 32.0 35.5 21.5 37.0 86-87 31.071671674275628 35.0 32.0 35.0 21.0 36.5 88-89 30.897019713937627 35.0 31.5 35.0 20.0 36.0 90-91 30.74216710239368 35.0 31.0 35.0 20.0 36.0 92-93 30.498665938287182 35.0 31.0 35.0 18.5 36.0 94-95 30.37582690581793 34.0 31.0 35.0 18.5 35.0 96-97 30.2728353472159 34.0 31.0 35.0 18.0 35.0 98-99 30.10335488122645 34.0 31.0 35.0 14.5 35.0 100-101 29.95387190059354 34.0 31.0 35.0 10.0 35.0 102-103 28.88995269868935 33.5 29.0 34.5 7.5 35.0 104-105 29.82118673829065 34.0 30.5 35.0 9.0 35.0 106-107 30.04439039406197 35.0 31.0 35.0 7.0 35.0 108-109 30.00976808917446 35.0 31.0 35.0 3.5 35.0 110-111 29.86387064327181 35.0 31.0 35.0 2.0 35.0 112-113 29.70755000021678 35.0 31.0 35.0 2.0 35.0 114-115 29.569284063663837 35.0 31.0 35.0 2.0 35.0 116-117 29.407702179502188 35.0 31.0 35.0 2.0 35.0 118-119 29.120220768353647 34.0 30.0 35.0 2.0 35.0 120-121 28.946677418935266 34.0 30.0 35.0 2.0 35.0 122-123 28.670142944474073 34.0 30.0 35.0 2.0 35.0 124-125 27.43567195175353 33.0 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7525688446917158 1101 2 0.7987327112248295 1101 3 0.9723271867919365 1101 4 0.9584892774341043 1101 5 0.7608603473878688 1101 6 0.8436098688051317 1101 7 0.7614648458981677 1101 8 0.9404616966307486 1101 9 1.2825872301204413 1101 10-11 1.0736767935390503 1101 12-13 0.9672554533006661 1101 14-15 1.1830896032937233 1101 16-17 1.0137841218527583 1101 18-19 1.0387266559277322 1101 20-21 1.0436230304240297 1101 22-23 1.0208183414003074 1101 24-25 0.9759335597666308 1101 26-27 1.0643070062673345 1101 28-29 0.9773055507774799 1101 30-31 1.0935025945697703 1101 32-33 1.0543055746961727 1101 34-35 0.9525210006877387 1101 36-37 0.977759984503578 1101 38-39 1.1303097319875022 1101 40-41 1.0765540078669105 1101 42-43 1.1483843171271246 1101 44-45 0.942931508372645 1101 46-47 0.8421028138703903 1101 48-49 0.8410039662367055 1101 50-51 0.842619224351786 1101 52-53 0.8347214987786913 1101 54-55 0.7633282291695735 1101 56-57 0.6594949355270145 1101 58-59 0.6783127516567831 1101 60-61 0.6726412114690845 1101 62-63 0.7016856464130967 1101 64-65 0.6329721431910045 1101 66-67 0.804360556245534 1101 68-69 0.6669921858636556 1101 70-71 0.5591786783693777 1101 72-73 0.5760588720122399 1101 74-75 0.663564723034888 1101 76-77 0.6894827865246285 1101 78-79 0.5237291662623349 1101 80-81 0.6263854737279857 1101 82-83 0.6867369189221613 1101 84-85 0.7419045456174622 1101 86-87 0.6881498784218607 1101 88-89 0.5546382691821812 1101 90-91 0.5653402388124 1101 92-93 0.7134092720274694 1101 94-95 0.5729748772023022 1101 96-97 0.5104373623889593 1101 98-99 0.5548606038814512 1101 100-101 0.5077985718636278 1101 102-103 0.4337981026466977 1101 104-105 0.42729181256935433 1101 106-107 0.4462426668713242 1101 108-109 0.4351196882036952 1101 110-111 0.42019354528978425 1101 112-113 0.4056965926004956 1101 114-115 0.43158820954909416 1101 116-117 0.33613794025127675 1101 118-119 0.2387145187119053 1101 120-121 0.14673679607883017 1101 122-123 0.0853642307724698 1101 124-125 -0.1449274255019013 1102 1 0.5790938502678529 1102 2 0.4584122057275728 1102 3 0.6144228080280456 1102 4 0.542527788143012 1102 5 0.4342530597842966 1102 6 0.49451442709344917 1102 7 0.6287949369116035 1102 8 0.7015942509368926 1102 9 1.1109428408026858 1102 10-11 0.6957598616658487 1102 12-13 0.6085367544719276 1102 14-15 0.8049441857906316 1102 16-17 0.6335680533288297 1102 18-19 0.534733597538775 1102 20-21 0.6978759912169821 1102 22-23 0.8402143913189732 1102 24-25 0.6762848564225834 1102 26-27 0.7202158078602068 1102 28-29 0.856625697225887 1102 30-31 0.8717470905141482 1102 32-33 0.6434588314324259 1102 34-35 0.36197778970301897 1102 36-37 0.36628517776433966 1102 38-39 0.5589480936367295 1102 40-41 0.5118745541651748 1102 42-43 0.5275320793279832 1102 44-45 0.42806158197765853 1102 46-47 0.556281911243353 1102 48-49 0.5591258007587143 1102 50-51 0.37437396678863877 1102 52-53 0.47564817121190117 1102 54-55 0.37310095396951226 1102 56-57 0.40423670596423733 1102 58-59 0.44513228609010724 1102 60-61 0.31511900212184685 1102 62-63 0.3782921179965655 1102 64-65 0.392949689037728 1102 66-67 0.25536058888633306 1102 68-69 0.3203635049329563 1102 70-71 0.3819027302900224 1102 72-73 0.40733005453417803 1102 74-75 0.3771335053696916 1102 76-77 0.281877003118133 1102 78-79 0.07866771994829236 1102 80-81 0.24827711713808043 1102 82-83 0.2651754916485949 1102 84-85 0.3871455843566558 1102 86-87 0.37885309734917527 1102 88-89 0.2671469053949771 1102 90-91 0.18865991740927868 1102 92-93 0.16212682842806103 1102 94-95 0.11311218618559948 1102 96-97 0.2210147510158187 1102 98-99 0.027643273246425792 1102 100-101 0.08094151410572437 1102 102-103 0.0890561282044473 1102 104-105 0.07593803521228182 1102 106-107 0.048266260732678035 1102 108-109 0.11502084096809995 1102 110-111 -0.006243602121525527 1102 112-113 -0.022910828286352825 1102 114-115 -0.015590128925165203 1102 116-117 0.06511182216772582 1102 118-119 0.0979738308613598 1102 120-121 0.06442892801296551 1102 122-123 0.10419151467674936 1102 124-125 -0.24113415603573785 1103 1 0.5512296844153184 1103 2 0.5988308499311721 1103 3 0.735925774249278 1103 4 0.4968353231057705 1103 5 0.40402998890537845 1103 6 0.33385976446029275 1103 7 0.402059625109942 1103 8 0.5292090266305038 1103 9 0.9248303849627248 1103 10-11 0.6123213813837651 1103 12-13 0.5880487536801269 1103 14-15 0.8069593713890271 1103 16-17 0.6497568680724797 1103 18-19 0.6988168698823856 1103 20-21 0.6505400019412946 1103 22-23 0.6341039284457253 1103 24-25 0.6462417248524446 1103 26-27 0.812066661233743 1103 28-29 0.7820177090651939 1103 30-31 1.0547896369869676 1103 32-33 0.9495418236834183 1103 34-35 0.9079454659375017 1103 36-37 0.8933509786575442 1103 38-39 0.8084198901279009 1103 40-41 0.7791100130827928 1103 42-43 0.8304918032212925 1103 44-45 0.7260303045196608 1103 46-47 0.7008118909164409 1103 48-49 0.6945148972108868 1103 50-51 0.7549917258726282 1103 52-53 0.6075034548965661 1103 54-55 0.5371370953435672 1103 56-57 0.585125198803361 1103 58-59 0.6865669056724855 1103 60-61 0.7058825440276806 1103 62-63 0.722022318781228 1103 64-65 0.7634132490553185 1103 66-67 0.5571666139748217 1103 68-69 0.7048460035207142 1103 70-71 0.7355922099169803 1103 72-73 0.4617796220402042 1103 74-75 0.616632052052573 1103 76-77 0.5464906335291815 1103 78-79 0.517509395575452 1103 80-81 0.4885043422753128 1103 82-83 0.46649088334398314 1103 84-85 0.6109331906622408 1103 86-87 0.5974022647132671 1103 88-89 0.6513560443592965 1103 90-91 0.6186904582775519 1103 92-93 0.5201883787811035 1103 94-95 0.5125107950140375 1103 96-97 0.5265818274750416 1103 98-99 0.5578554030074123 1103 100-101 0.3497023331881124 1103 102-103 0.35101275020666733 1103 104-105 0.2560271531427176 1103 106-107 0.22538999905345847 1103 108-109 0.27888545927794084 1103 110-111 0.40235194211660996 1103 112-113 0.3310782696470902 1103 114-115 0.36097369347725916 1103 116-117 0.38857974583979527 1103 118-119 0.34052865259520004 1103 120-121 0.2581525304618886 1103 122-123 0.253340834341202 1103 124-125 -0.058835773907265576 1104 1 0.5730731780935336 1104 2 0.6148075774942967 1104 3 0.6540418670941719 1104 4 0.6008304938302658 1104 5 0.6591842453950321 1104 6 0.6275057073697496 1104 7 0.6554220521285217 1104 8 0.8414506938229849 1104 9 1.337030583922342 1104 10-11 1.0603955257051467 1104 12-13 1.0437048982143367 1104 14-15 0.9658283779187826 1104 16-17 0.8439820164053842 1104 18-19 0.9041648279093764 1104 20-21 1.0615339807287754 1104 22-23 0.8609613740967532 1104 24-25 1.017779041082406 1104 26-27 0.9347576952744205 1104 28-29 0.8439345522308273 1104 30-31 1.02225732174427 1104 32-33 0.9985529742746309 1104 34-35 0.8988447427076593 1104 36-37 0.7919977204818451 1104 38-39 0.931166330248054 1104 40-41 0.860169735185984 1104 42-43 0.8382602692200152 1104 44-45 0.6840830366672108 1104 46-47 0.8029580995553331 1104 48-49 0.7865078240676482 1104 50-51 0.7695230296796964 1104 52-53 0.5755543945868169 1104 54-55 0.5853348909663936 1104 56-57 0.5281008851088913 1104 58-59 0.6057349292387499 1104 60-61 0.7345671974226278 1104 62-63 0.740263004673956 1104 64-65 0.6718828766354292 1104 66-67 0.5218628960542375 1104 68-69 0.5546371398664363 1104 70-71 0.6560843289122857 1104 72-73 0.64493532630798 1104 74-75 0.6483787938454881 1104 76-77 0.6811198726828565 1104 78-79 0.5658837686470335 1104 80-81 0.6160370514819249 1104 82-83 0.6114236128295296 1104 84-85 0.6408129202303492 1104 86-87 0.7105235858202228 1104 88-89 0.6798437158003026 1104 90-91 0.573012148172392 1104 92-93 0.6264173719506161 1104 94-95 0.6559943337579561 1104 96-97 0.5972851644906036 1104 98-99 0.6295466319895944 1104 100-101 0.5612579695245827 1104 102-103 0.554381000357111 1104 104-105 0.5247039460944372 1104 106-107 0.5159870380144476 1104 108-109 0.24696292482493476 1104 110-111 0.4490112380481719 1104 112-113 0.48741821623751136 1104 114-115 0.5075549500243604 1104 116-117 0.41994303989288895 1104 118-119 0.4505285552814051 1104 120-121 0.37104265282927784 1104 122-123 0.6051912561337147 1104 124-125 0.344825924419343 1105 1 0.49645907372707043 1105 2 0.5169226056558713 1105 3 0.5682399562262219 1105 4 0.34335491694335474 1105 5 0.4060948297887137 1105 6 0.42941836619833396 1105 7 0.5119732369442573 1105 8 0.5498481257024181 1105 9 0.9890821851174536 1105 10-11 0.668047205698933 1105 12-13 0.7262524833631758 1105 14-15 0.4762344298960599 1105 16-17 0.6961846484006244 1105 18-19 0.6373817541042683 1105 20-21 0.8097080302943382 1105 22-23 0.8472478669884609 1105 24-25 0.7618821240806923 1105 26-27 0.7286327396732943 1105 28-29 0.5880764376085992 1105 30-31 0.8508230365587224 1105 32-33 0.7225658268166342 1105 34-35 0.5166992099062213 1105 36-37 0.5306232506566673 1105 38-39 0.6937944154103235 1105 40-41 0.6272196499699945 1105 42-43 0.5836017450834419 1105 44-45 0.5655698244569933 1105 46-47 0.6853060016897601 1105 48-49 0.4744971444588941 1105 50-51 0.5486142182562759 1105 52-53 0.2899545545720059 1105 54-55 0.2958389935718202 1105 56-57 0.38689260885982435 1105 58-59 0.46515943025507767 1105 60-61 0.33032997758327554 1105 62-63 0.469671925042519 1105 64-65 0.5280111155148148 1105 66-67 0.3342239909976925 1105 68-69 0.5198852810832264 1105 70-71 0.5422600857825444 1105 72-73 0.49921433332523435 1105 74-75 0.46383318463320933 1105 76-77 0.4084952498867551 1105 78-79 0.3995287149355846 1105 80-81 0.4203535177193771 1105 82-83 0.4315336921953197 1105 84-85 0.5290647536427784 1105 86-87 0.5050584199102026 1105 88-89 0.46573143962287133 1105 90-91 0.3663466455771136 1105 92-93 0.5358494842029167 1105 94-95 0.41138174566909314 1105 96-97 0.4282524481151029 1105 98-99 0.5200646736036525 1105 100-101 0.30882670197023643 1105 102-103 0.3741393212289097 1105 104-105 0.37462275957737035 1105 106-107 0.29249652519623126 1105 108-109 0.37690761651018434 1105 110-111 0.37809824790695856 1105 112-113 0.3044836519733103 1105 114-115 0.28495435485555376 1105 116-117 0.22579503567835246 1105 118-119 0.0920959027805921 1105 120-121 0.09028982697137167 1105 122-123 0.18854264749749916 1105 124-125 -0.11613783714301817 1106 1 0.41633711662315775 1106 2 0.4240222944300278 1106 3 0.4493245421028398 1106 4 0.3294812408594936 1106 5 0.2936934449543429 1106 6 0.11532092114537562 1106 7 0.05045607127078 1106 8 0.412461819639546 1106 9 0.7599331103508788 1106 10-11 0.042850276943113386 1106 12-13 0.3431485484069654 1106 14-15 0.6508369534792564 1106 16-17 0.5388343504440982 1106 18-19 0.3622385057749966 1106 20-21 0.2891767750167702 1106 22-23 0.36558636731415817 1106 24-25 0.4507551970136916 1106 26-27 0.5040057884802636 1106 28-29 0.36415155401670773 1106 30-31 0.4463956076702118 1106 32-33 0.40825185060489844 1106 34-35 0.2960717520664673 1106 36-37 0.3187954622902396 1106 38-39 0.5141727719448568 1106 40-41 0.4270278479964844 1106 42-43 0.3870047222360853 1106 44-45 0.2356770646799191 1106 46-47 0.36386640121688885 1106 48-49 0.36316605082576103 1106 50-51 0.33943124641135825 1106 52-53 0.19755516013547236 1106 54-55 0.23374973930754095 1106 56-57 0.25202445763783743 1106 58-59 0.3304541297653145 1106 60-61 0.24023582573400404 1106 62-63 0.3951233834612964 1106 64-65 0.434960016573946 1106 66-67 0.36057173159456823 1106 68-69 0.340821552163348 1106 70-71 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18575.0 3 3780.0 4 2685.0 5 2645.0 6 3152.0 7 3260.0 8 3239.0 9 3237.0 10 3734.0 11 4024.0 12 4374.0 13 4625.0 14 4581.0 15 4446.0 16 4232.0 17 4082.0 18 3885.0 19 3816.0 20 3825.0 21 4184.0 22 4783.0 23 6472.0 24 9407.0 25 12767.0 26 15312.0 27 15823.0 28 18599.0 29 22336.0 30 26755.0 31 32877.0 32 41075.0 33 54550.0 34 73779.0 35 105646.0 36 184789.0 37 311817.0 38 125896.0 39 181.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.77895982010739 20.069204152249135 12.820472764859495 23.33136326278398 2 15.000633000300025 21.76922716733233 39.78441570603813 23.445724126329516 3 17.470355388490738 28.70526210822505 29.468066195821358 24.356316307462855 4 11.65261622613434 17.553714536439518 38.652007498963776 32.14166173846237 5 11.992286034131872 40.97133883612418 34.78733265523966 12.249042474504284 6 29.546617275472105 38.9435174924257 18.565834404227388 12.944030827874808 7 27.610202177509702 33.9842218992685 22.335169313692504 16.070406609529293 8 26.093878442920616 35.81149044948302 20.967520958416145 17.12711014918022 9 27.31281200167528 16.65916605317718 20.32752967945066 35.70049226569687 10-11 23.46293598348856 28.605720033994135 28.515358065802936 19.415985916714362 12-13 24.190821805852732 26.36707140209283 28.03639367077906 21.405713121275383 14-15 22.125920103156478 27.258900129595965 25.500401274887825 25.114778492359736 16-17 21.47913506117032 30.441951605707352 26.542850441093137 21.536062892029193 18-19 21.67595832261942 29.486196069623027 27.821676522022678 21.016169085734877 20-21 22.728573621507426 28.569285193308545 27.438157656239564 21.263983528944465 22-23 22.56574890927964 28.568157989900595 27.271217556977184 21.59487554384258 24-25 22.16367133570601 28.874288023532515 27.35398845574819 21.608052185013285 26-27 21.624345100814256 29.078542807848688 27.409398114965672 21.88771397637138 28-29 21.886765291871377 28.534275792424857 27.748560794378196 21.830398121325565 30-31 21.810495257654708 28.688023409909313 27.367313202939076 22.134168129496903 32-33 21.769369044387926 28.767866294259807 27.130969919850433 22.33179474150183 34-35 22.542038375626067 28.521754512535207 27.21169721270308 21.724509899135647 36-37 22.47763361840037 28.0394107891913 27.235326634799854 22.247628957608473 38-39 21.882518204107114 28.58930363735294 27.20863131561662 22.319546842923323 40-41 22.055208366753927 28.125804804067876 27.72202620290297 22.096960626275223 42-43 21.818304005965917 28.091014921296285 27.372893664898424 22.71778740783937 44-45 21.720012209122853 28.42323687432581 27.47555574054227 22.381195176009076 46-47 22.271306751668796 28.45019102933857 26.974677990263835 22.3038242287288 48-49 21.58364685901252 28.394695045781184 27.333079824497887 22.688578270708412 50-51 22.066441783227393 28.198172027159448 27.2491089933142 22.48627719629896 52-53 22.20069192749807 28.08055808605171 27.23982151583199 22.47892847061823 54-55 21.676014230554934 28.48265949213135 27.35900065255896 22.482325624754747 56-57 21.742580405141144 28.548410455378363 27.227829670401142 22.48117946907935 58-59 21.834133464684378 27.646243660809056 27.532554477151162 22.987068397355404 60-61 21.601869023527996 28.58070158626781 27.263617564762594 22.553811825441596 62-63 21.526693128965984 28.113360050022244 27.861991332830915 22.497955488180857 64-65 21.591894535653704 27.987561006913246 27.440753485242137 22.97979097219091 66-67 21.400383230645616 28.22980656782224 27.158512572506172 23.21129762902597 68-69 21.7588017488805 27.793289367047663 27.64893691290417 22.798971971167667 70-71 21.836376328374644 27.64662524551355 27.384700766132354 23.132297659979447 72-73 21.55884217491564 27.818354634014774 27.103104433033586 23.519698758035997 74-75 21.309305194509548 28.26911110773854 27.245342416154816 23.176241281597097 76-77 21.753003214586435 27.698557294995325 27.609325856045146 22.939113634373097 78-79 21.65500728873719 27.603934964172016 27.555459008332832 23.185598738757957 80-81 21.702932894397936 28.080389005917127 27.16740974058467 23.049268359100264 82-83 21.867579385935244 27.642454108190712 27.432140137619054 23.05782636825499 84-85 21.60042945458314 27.63382405804228 27.41801272319909 23.34773376417549 86-87 21.720542850960552 27.878285658037893 27.326276461642735 23.074895029358824 88-89 21.844995728775046 27.613837901592714 27.292216830794448 23.248949538837792 90-91 21.613988927354846 27.63508661777867 27.43415210317818 23.3167723516883 92-93 21.779685232407438 27.498565867960988 27.355477864069 23.366271035562573 94-95 21.57664977964782 27.050376619254667 27.70627340320547 23.666700197892045 96-97 21.87463550657053 26.814488941730048 27.782149892272546 23.52872565942688 98-99 21.5811410858817 27.19334948297026 27.51676135464438 23.708748076503667 100-101 21.411103171244424 27.02105259506449 27.79942451383734 23.768419719853753 102-103 21.458242817015247 26.76564273740425 27.77647694446396 23.99963750111655 104-105 21.094750680977974 27.141644285378554 27.58418240583599 24.17942262780749 106-107 21.310869104299712 27.038198236791 27.492203092054464 24.158729566854827 108-109 21.36429105962763 27.13905656139791 27.138492915842516 24.358159463131944 110-111 21.24173909725601 27.28493469756923 27.406685727119868 24.066640478054897 112-113 21.158833361878518 27.234535194045833 27.77054873963972 23.83608270443592 114-115 21.22180276701677 27.689203639287026 27.214432959292612 23.874560634403593 116-117 20.921770086483697 27.580516127074016 27.501815745713216 23.995898040729074 118-119 20.847345544160405 27.52893249981317 27.33597675319913 24.287745202827296 120-121 20.797802837351245 27.739655481219128 27.38035711265489 24.08218456877474 122-123 20.768648641616053 27.842913613763166 27.29426917881142 24.09416856580936 124-125 20.84486746146044 27.806628187221822 27.257770272325654 24.09073407899208 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2028.0 1 1567.0 2 946.5 3 665.0 4 454.0 5 343.0 6 296.0 7 299.0 8 316.5 9 300.0 10 277.5 11 234.0 12 204.0 13 210.0 14 222.0 15 237.0 16 259.0 17 277.5 18 326.5 19 409.5 20 527.5 21 656.0 22 870.5 23 1196.0 24 1581.5 25 2219.5 26 3286.0 27 4718.0 28 6319.0 29 8416.0 30 11612.0 31 15290.0 32 20879.0 33 25841.0 34 29377.0 35 32748.5 36 37564.0 37 42386.5 38 44347.0 39 46033.5 40 45014.5 41 43415.0 42 42444.5 43 42624.0 44 43577.0 45 44359.5 46 43877.0 47 41782.0 48 39610.5 49 37697.0 50 37252.0 51 36294.5 52 35281.5 53 35127.5 54 34945.5 55 32494.0 56 28751.5 57 25842.0 58 24220.0 59 21447.0 60 16633.0 61 13451.5 62 11276.0 63 8358.5 64 6021.0 65 4616.0 66 3262.5 67 2339.5 68 1787.5 69 1275.5 70 890.5 71 632.5 72 507.5 73 399.5 74 307.5 75 234.0 76 150.0 77 113.5 78 89.0 79 40.5 80 21.5 81 14.5 82 8.0 83 6.0 84 4.5 85 3.0 86 3.5 87 3.0 88 3.0 89 1.5 90 0.5 91 0.5 92 1.0 93 1.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08688526722422382 2 6.069829047600467E-4 3 0.0 4 0.0016475250272058408 5 0.0010405421224457942 6 2.6013553061144854E-4 7 8.671184353714952E-5 8 7.804065918343456E-4 9 5.202710612228971E-4 10-11 0.0091047435714007 12-13 0.0019510164795858642 14-15 0.0039453888809403035 16-17 0.0026447112278830606 18-19 0.012963420608803853 20-21 0.016648673959132707 22-23 0.009364879102012148 24-25 0.010145285693846493 26-27 0.009494946867317873 28-29 0.007934133683649182 30-31 0.01985701217000724 32-33 0.0022545079319658875 34-35 0.0011272539659829437 36-37 6.503388265286215E-4 38-39 2.167796088428738E-4 40-41 0.0012139658095200933 42-43 0.002384575697271612 44-45 7.804065918343456E-4 46-47 0.0016475250272058408 48-49 0.008367692901334928 50-51 0.004769151394543224 52-53 0.007760709996574882 54-55 0.00802084552718633 56-57 0.010058573850309345 58-59 0.008757896197252101 60-61 0.011229183738060863 62-63 0.013700471278869624 64-65 0.008411048823103503 66-67 0.013310267982952451 68-69 0.014264098261861096 70-71 0.004335592176857476 72-73 0.013180200217646727 74-75 0.013223556139415303 76-77 0.005549557986377569 78-79 0.008324336979566353 80-81 0.013180200217646727 82-83 0.01794935161218995 84-85 0.0133969798264896 86-87 0.015998335132604086 88-89 0.015608131836686913 90-91 0.011532675190440886 92-93 0.009408235023780723 94-95 0.007890777761880607 96-97 0.008974675806094975 98-99 0.00619989681290619 100-101 0.011532675190440886 102-103 0.011966234408126634 104-105 0.01075226859860654 106-107 0.005506202064608995 108-109 0.0032950500544116816 110-111 0.006243252734674765 112-113 0.008887963962557827 114-115 0.014047318653018222 116-117 0.01192287848635806 118-119 0.21357127063199927 120-121 0.026317044513524877 122-123 0.027097451105359223 124-125 0.23186746961833782 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1153245.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.674655494086856 #Duplication Level Percentage of deduplicated Percentage of total 1 80.20154678386614 35.02774925878243 2 8.7448792497998 7.638591771447898 3 3.462459129232985 4.536651288948198 4 1.8241479313211382 3.1867612988280767 5 1.1282535428661158 2.463804239733028 6 0.7695866337350032 2.016685866073816 7 0.5231725201373211 1.5994565706679502 8 0.41641451129101137 1.454940825869877 9 0.315229603934315 1.2390789918031788 >10 2.163589989881261 18.679357861763698 >50 0.28264821401020296 8.582958708428102 >100 0.16368046287210186 12.379435017577373 >500 0.003992206411514679 1.0151136714413447 >1k 3.9922064115146795E-4 0.17941462863502983 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.671184353714952E-5 0.0 18-19 0.0 0.0 0.0 8.671184353714952E-5 0.0 20-21 0.0 0.0 0.0 8.671184353714952E-5 0.0 22-23 0.0 0.0 0.0 2.167796088428738E-4 0.0 24-25 0.0 0.0 0.0 0.0012139658095200933 0.0 26-27 0.0 0.0 0.0 0.0029915586020316585 0.0 28-29 0.0 0.0 0.0 0.007543930387732009 0.0 30-31 0.0 0.0 0.0 0.022805214850270324 0.0 32-33 0.0 0.0 0.0 0.058964053605261676 0.0 34-35 0.0 0.0 0.0 0.11224848145884006 0.0 36-37 0.0 0.0 0.0 0.191373038686489 0.0 38-39 0.0 0.0 0.0 0.31788561840719015 0.0 40-41 0.0 0.0 0.0 0.45740497465846375 0.0 42-43 0.0 0.0 0.0 0.5953635177260685 0.0 44-45 0.0 0.0 0.0 0.7618502573173958 0.0 46-47 0.0 0.0 0.0 0.9559980749970735 0.0 48-49 0.0 0.0 0.0 1.17026304037737 0.0 50-51 0.0 0.0 0.0 1.4052521363630452 0.0 52-53 0.0 0.0 0.0 1.633954623692277 0.0 54-55 0.0 0.0 0.0 1.8759240230826926 0.0 56-57 0.0 0.0 0.0 2.1538788375410256 0.0 58-59 0.0 0.0 0.0 2.462833136063889 0.0 60-61 0.0 0.0 0.0 2.80595190094039 0.0 62-63 0.0 0.0 0.0 3.1434777519087445 0.0 64-65 0.0 0.0 0.0 3.482911263434916 0.0 66-67 0.0 0.0 0.0 3.860497986117434 0.0 68-69 0.0 0.0 0.0 4.295791440673925 0.0 70-71 0.0 0.0 0.0 4.760350142424203 0.0 72-73 0.0 0.0 0.0 5.230848605456776 0.0 74-75 0.0 0.0 0.0 5.6850452419043656 0.0 76-77 0.0 0.0 0.0 6.159315670130805 0.0 78-79 0.0 0.0 0.0 6.710109300278779 0.0 80-81 0.0 0.0 0.0 7.315357968168082 0.0 82-83 0.0 0.0 0.0 7.949785171407637 0.0 84-85 0.0 0.0 0.0 8.563661667728887 0.0 86-87 0.0 0.0 0.0 9.205849580965015 0.0 88-89 0.0 0.0 0.0 9.876435622959562 0.0 90-91 0.0 0.0 0.0 10.620813443804222 0.0 92-93 0.0 0.0 0.0 11.383877666931138 0.0 94-95 0.0 0.0 0.0 12.157260599438974 0.0 96-97 0.0 0.0 0.0 12.942696478198474 0.0 98-99 0.0 0.0 0.0 13.747902657284445 0.0 100-101 0.0 0.0 0.0 14.629198479074265 0.0 102-103 0.0 0.0 0.0 15.573447099272054 0.0 104-105 0.0 4.335592176857476E-5 0.0 16.510888839752177 0.0 106-107 0.0 8.671184353714952E-5 0.0 17.41763458762015 0.0 108-109 0.0 8.671184353714952E-5 0.0 18.35308195569892 0.0 110-111 0.0 8.671184353714952E-5 0.0 19.344068259563233 0.0 112-113 0.0 8.671184353714952E-5 0.0 20.375028723298172 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1340 0.0 66.181465 1 GTATCAA 2480 0.0 56.158962 1 TCAACGC 2870 0.0 46.005066 4 CAACGCA 2905 0.0 45.458675 5 ATCAACG 2905 0.0 45.242134 3 CCGTTGC 100 2.2306267E-7 41.63793 9 AACGCAG 3160 0.0 41.41025 6 GTACATG 7970 0.0 41.07327 1 TATCAAC 3405 0.0 39.297264 2 TACATGG 8510 0.0 37.736458 2 ACATGGG 8240 0.0 36.66346 3 CATGGGA 6080 0.0 34.139442 4 ACGCAGA 3850 0.0 33.679688 7 CGCAGAG 3980 0.0 32.43015 8 CATGGGG 2305 0.0 31.479187 4 ATGGGAT 2605 0.0 30.370632 5 ATGGGGA 1320 0.0 28.841375 5 TCCGTTG 170 4.4375702E-7 27.990673 8 GAGTACA 5595 0.0 27.020197 1 GCAGAGT 4650 0.0 26.735262 9 >>END_MODULE