FastQCFastQC Report
Thu 26 May 2016
SRR1364440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364440_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153245
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35230.30548582478137776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29530.25606007396520253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20810.18044734640080815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.084.0118261
GTATCAA42950.060.1961821
ATCAACG54600.046.790343
TCAACGC55100.046.3657464
CAACGCA56300.045.4457865
AACGCAG59150.043.354816
TATCAAC58950.043.2403532
GTACATG78550.039.1332471
TACATGG83350.036.727112
ACGCAGA70000.036.6348157
CGCAGAG71150.035.955948
CATGGGA62850.035.721334
ACATGGG84600.035.618183
CCGTTGC902.3942874E-433.0525259
GCAGAGT81050.031.0502039
ATGGGAT26800.030.8612565
CATATAG4300.030.468133
TGGTATC8850.028.2641052
ATGGGAG23400.028.225445
GGAACGC2353.4197E-1027.8485078
Produced by FastQC (version 0.11.5)