##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364440_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1153245 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.904660328030904 33.0 31.0 34.0 30.0 34.0 2 31.811020208195135 33.0 31.0 34.0 30.0 34.0 3 32.03139228871576 33.0 31.0 34.0 30.0 34.0 4 35.74707716053397 37.0 35.0 37.0 33.0 37.0 5 35.70083806996779 37.0 35.0 37.0 33.0 37.0 6 35.76418714150072 37.0 35.0 37.0 33.0 37.0 7 35.66967816899271 37.0 35.0 37.0 33.0 37.0 8 35.676648934094665 37.0 35.0 37.0 33.0 37.0 9 37.30107912889282 39.0 37.0 39.0 34.0 39.0 10-11 37.253236302780415 39.0 37.0 39.0 33.0 39.0 12-13 37.236846030115025 39.0 37.0 39.0 33.0 39.0 14-15 38.483490932108964 40.0 38.0 41.0 33.0 41.0 16-17 38.50159983351326 40.0 38.0 41.0 33.5 41.0 18-19 38.59999046169721 40.0 38.0 41.0 34.0 41.0 20-21 38.61561290098808 40.0 38.0 41.0 34.0 41.0 22-23 38.57591578545755 40.0 38.0 41.0 34.0 41.0 24-25 38.522418480028094 40.0 38.0 41.0 34.0 41.0 26-27 38.40000130067765 40.0 38.0 41.0 34.0 41.0 28-29 38.32043971575858 40.0 38.0 41.0 33.5 41.0 30-31 38.17348915451617 40.0 37.5 41.0 33.0 41.0 32-33 38.01532328343067 40.0 37.0 41.0 33.0 41.0 34-35 37.905212682474236 40.0 37.0 41.0 33.0 41.0 36-37 37.78200295687387 40.0 37.0 41.0 33.0 41.0 38-39 37.67561576247892 40.0 37.0 41.0 33.0 41.0 40-41 37.563186486826304 40.0 37.0 41.0 32.0 41.0 42-43 37.406662070938964 40.0 37.0 41.0 32.0 41.0 44-45 37.19891003212673 40.0 36.0 41.0 31.0 41.0 46-47 36.93694661585353 40.0 36.0 41.0 31.0 41.0 48-49 36.675856821403954 40.0 36.0 41.0 30.0 41.0 50-51 36.47461554136372 39.5 35.5 41.0 30.0 41.0 52-53 36.35922895828727 39.0 35.0 41.0 30.0 41.0 54-55 36.21104752242585 39.0 35.0 41.0 29.5 41.0 56-57 36.01355306114486 39.0 35.0 41.0 29.0 41.0 58-59 35.809563015664494 39.0 35.0 40.5 28.5 41.0 60-61 35.57690343335544 38.0 35.0 40.0 28.0 41.0 62-63 35.6218180005116 38.0 35.0 40.0 29.0 41.0 64-65 35.41240109430346 38.0 35.0 40.0 29.0 41.0 66-67 35.133410506874085 37.0 34.0 40.0 28.0 41.0 68-69 34.807821408287055 37.0 34.0 39.5 28.0 41.0 70-71 34.46547437881803 36.0 34.0 39.0 28.0 41.0 72-73 34.11501112079394 36.0 34.0 39.0 28.0 41.0 74-75 33.772855290939916 35.0 34.0 38.0 27.5 40.0 76-77 33.45442252080001 35.0 33.5 37.0 27.0 39.0 78-79 33.12303673547251 35.0 33.0 37.0 26.5 39.0 80-81 32.827806320426276 35.0 33.0 36.0 26.0 39.0 82-83 32.54189612788262 35.0 33.0 36.0 26.0 37.0 84-85 32.298076731310346 35.0 33.0 35.5 26.0 37.0 86-87 32.11042753274456 35.0 33.0 35.0 26.0 36.5 88-89 31.915047539768217 35.0 33.0 35.0 25.5 36.0 90-91 31.78017897324506 35.0 33.0 35.0 25.0 36.0 92-93 31.656119905137245 35.0 33.0 35.0 25.0 36.0 94-95 31.533349808583605 35.0 32.0 35.0 25.0 35.5 96-97 31.47443951632134 35.0 32.0 35.0 25.0 35.0 98-99 31.39448295895495 35.0 32.0 35.0 25.0 35.0 100-101 31.386197208745756 35.0 32.5 35.0 24.5 35.0 102-103 30.254705201409934 33.5 30.0 34.5 21.5 35.0 104-105 31.216353853691103 34.5 32.0 35.0 24.0 35.0 106-107 31.398616946095583 35.0 33.0 35.0 25.0 35.0 108-109 31.403096046373495 35.0 33.0 35.0 25.0 35.0 110-111 31.327083143651176 35.0 33.0 35.0 24.0 35.0 112-113 31.251361159163924 35.0 33.0 35.0 24.0 35.0 114-115 31.130195231715724 35.0 33.0 35.0 23.5 35.0 116-117 31.030892394937762 35.0 33.0 35.0 23.0 35.0 118-119 30.917492813755967 35.0 33.0 35.0 21.5 35.0 120-121 30.76341020338263 35.0 32.5 35.0 19.0 35.0 122-123 30.562499295466274 35.0 32.0 35.0 14.0 35.0 124-125 29.508256701741608 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3843382527902328 1101 2 0.4905899142651009 1101 3 0.3582120619451814 1101 4 0.27316221275000885 1101 5 0.3128688030784801 1101 6 0.3497146538803335 1101 7 0.35967535335484513 1101 8 0.3436159741802598 1101 9 0.3899334844201974 1101 10-11 0.4610293823780651 1101 12-13 0.44015654652437775 1101 14-15 0.4881703418618528 1101 16-17 0.49908338765089155 1101 18-19 0.35509861147739485 1101 20-21 0.30435318441339376 1101 22-23 0.410889610785631 1101 24-25 0.46182942540762184 1101 26-27 0.4379173323630354 1101 28-29 0.4404033128699112 1101 30-31 0.4650777079065165 1101 32-33 0.38217241522217194 1101 34-35 0.4319711443626062 1101 36-37 0.3778571031977265 1101 38-39 0.4572037764882282 1101 40-41 0.3847571309737674 1101 42-43 0.24761419649745164 1101 44-45 0.3540926321418567 1101 46-47 0.5386478557968033 1101 48-49 0.46804229890484095 1101 50-51 0.42458701289898926 1101 52-53 0.448825640719825 1101 54-55 0.37769280467061606 1101 56-57 0.4058370319062874 1101 58-59 0.44277110408154385 1101 60-61 0.4172426717432671 1101 62-63 0.5770456495667773 1101 64-65 0.4664978089154346 1101 66-67 0.5947150409426598 1101 68-69 0.6106835767448544 1101 70-71 0.5419933294155541 1101 72-73 0.5493547279564908 1101 74-75 0.4626525429763291 1101 76-77 0.4236815999717862 1101 78-79 0.4844180432006695 1101 80-81 0.34123849836318954 1101 82-83 0.3627958237980451 1101 84-85 0.32543606697455374 1101 86-87 0.40891719009758987 1101 88-89 0.47418993524602726 1101 90-91 0.43797702824011253 1101 92-93 0.49323654021960195 1101 94-95 0.4746009325122884 1101 96-97 0.2892551916715185 1101 98-99 0.4030300496543333 1101 100-101 0.3794903021483478 1101 102-103 0.5063495675914425 1101 104-105 0.42931683583561053 1101 106-107 0.4989250161704355 1101 108-109 0.5256513793122046 1101 110-111 0.5195252644079851 1101 112-113 0.3770553152338856 1101 114-115 0.415362993912165 1101 116-117 0.3576077119852279 1101 118-119 0.47801338749417255 1101 120-121 0.5390654797839716 1101 122-123 0.5625977396982762 1101 124-125 0.38806592648309746 1102 1 0.32717604431341485 1102 2 0.5132467125286055 1102 3 0.33973260661139904 1102 4 0.264624602383293 1102 5 0.2361819535145031 1102 6 0.28622556950929834 1102 7 0.3453065993087421 1102 8 0.26270894063095085 1102 9 0.2952098704303481 1102 10-11 0.3899458708803465 1102 12-13 0.3560150132769948 1102 14-15 0.4440352143723132 1102 16-17 0.5052695000767002 1102 18-19 0.4599486949834457 1102 20-21 0.44669837179881 1102 22-23 0.36176044024286824 1102 24-25 0.39508000070179605 1102 26-27 0.4140426240902215 1102 28-29 0.44294487549401396 1102 30-31 0.5132599551170927 1102 32-33 0.5925083979007866 1102 34-35 0.5553639247607194 1102 36-37 0.6104408540613449 1102 38-39 0.4966580086405088 1102 40-41 0.3901904648511163 1102 42-43 0.48155866088905697 1102 44-45 0.3988571151387177 1102 46-47 0.4704853562728175 1102 48-49 0.44105515394159056 1102 50-51 0.5030646865888642 1102 52-53 0.4599901558134789 1102 54-55 0.3343417365002921 1102 56-57 0.3771529051294067 1102 58-59 0.4464377544278051 1102 60-61 0.369980487203037 1102 62-63 0.41016919773100113 1102 64-65 0.5533298284563983 1102 66-67 0.4227435635654544 1102 68-69 0.31589087305263774 1102 70-71 0.4079725671439718 1102 72-73 0.39750290632760965 1102 74-75 0.4843732981757398 1102 76-77 0.4103957726090002 1102 78-79 0.33956223395353646 1102 80-81 0.3242707202771413 1102 82-83 0.3126972893701492 1102 84-85 0.14172852042060669 1102 86-87 0.36448081518530984 1102 88-89 0.30699251550160866 1102 90-91 0.27397500723031243 1102 92-93 0.3293079610194063 1102 94-95 0.4755464958109741 1102 96-97 0.35642316669042273 1102 98-99 0.40244081513559493 1102 100-101 0.3460470781073113 1102 102-103 0.3338901781920889 1102 104-105 0.3261701943023638 1102 106-107 0.27223701057797456 1102 108-109 0.31175992902686644 1102 110-111 0.34595506763833583 1102 112-113 0.24612488910818442 1102 114-115 0.25425716426452283 1102 116-117 0.28848706885258224 1102 118-119 0.14617813107345512 1102 120-121 0.06374056777814019 1102 122-123 -0.0951352068415261 1102 124-125 -0.2008000231739011 1103 1 0.3348624321487783 1103 2 0.4241590805703055 1103 3 0.35596114602938655 1103 4 0.15080970724633147 1103 5 0.17848220068277243 1103 6 0.1562252261265229 1103 7 0.25472558891501507 1103 8 0.23176308266888412 1103 9 0.3574106308548721 1103 10-11 0.45317896786308864 1103 12-13 0.39206397696797524 1103 14-15 0.456647630150961 1103 16-17 0.4719192893675981 1103 18-19 0.3593776022923194 1103 20-21 0.29181270816446414 1103 22-23 0.2669445439094531 1103 24-25 0.3864223782437577 1103 26-27 0.38448561480609555 1103 28-29 0.4216793820293887 1103 30-31 0.49699000777584956 1103 32-33 0.5260284820901617 1103 34-35 0.3972817714409871 1103 36-37 0.42484279908913436 1103 38-39 0.30547291387696873 1103 40-41 0.25993342600913394 1103 42-43 0.3322346199874531 1103 44-45 0.292592433000074 1103 46-47 0.33608264368352536 1103 48-49 0.41798269276908684 1103 50-51 0.4650416085729745 1103 52-53 0.3119361262165796 1103 54-55 0.30982774095888743 1103 56-57 0.20546067440041327 1103 58-59 0.19014543334202472 1103 60-61 0.06486384815288204 1103 62-63 0.10674590708710241 1103 64-65 0.21283516873189967 1103 66-67 0.19029934475117471 1103 68-69 0.26287290874693525 1103 70-71 0.2874125569636874 1103 72-73 0.40928985683941477 1103 74-75 0.3135275733249898 1103 76-77 0.2977153872392577 1103 78-79 0.31484540691813123 1103 80-81 0.3172337298723349 1103 82-83 0.27254725802805524 1103 84-85 0.3722812724315787 1103 86-87 0.3664774850456638 1103 88-89 0.314388374574623 1103 90-91 0.2683571639838789 1103 92-93 0.03759520138668293 1103 94-95 0.1624078290922384 1103 96-97 0.270842078867247 1103 98-99 0.30516703873972517 1103 100-101 0.20760843959982367 1103 102-103 0.3385611939250772 1103 104-105 0.433541949250948 1103 106-107 0.30505043320878755 1103 108-109 0.2990520730348756 1103 110-111 0.28579760306904234 1103 112-113 0.3458562089167252 1103 114-115 0.280143849294312 1103 116-117 0.127399642923951 1103 118-119 0.04365413299370502 1103 120-121 0.04590654554713325 1103 122-123 0.20901895048030283 1103 124-125 -0.13306356204988035 1104 1 0.35002486299712743 1104 2 0.4046542571022371 1104 3 0.19211893375410227 1104 4 0.10572987697414504 1104 5 0.18568593276094703 1104 6 0.18210065003011522 1104 7 0.2525178214535515 1104 8 0.2046028027692941 1104 9 0.2907864683420911 1104 10-11 0.2931852022242154 1104 12-13 0.19204598961185582 1104 14-15 0.16680712745928616 1104 16-17 0.22212137244783747 1104 18-19 0.2576641261211705 1104 20-21 0.304859191824697 1104 22-23 0.24342113162324353 1104 24-25 0.3024242504355854 1104 26-27 0.2372140581526736 1104 28-29 0.1544239983007003 1104 30-31 0.34065837994523207 1104 32-33 0.24474955281814204 1104 34-35 0.1463875047616341 1104 36-37 0.1684399438396369 1104 38-39 0.0413574695814205 1104 40-41 0.1253655573287631 1104 42-43 0.14615447925287128 1104 44-45 0.20060641228354115 1104 46-47 0.2841950856915645 1104 48-49 0.0926989367328872 1104 50-51 0.3198896091355081 1104 52-53 0.2946874131915038 1104 54-55 0.2423453335662984 1104 56-57 0.21132806834643247 1104 58-59 0.01341620646244479 1104 60-61 0.04428810207784295 1104 62-63 0.36602391407549106 1104 64-65 0.3684474592757283 1104 66-67 0.2970885200669926 1104 68-69 0.4280453378028355 1104 70-71 0.35345848719094874 1104 72-73 0.2504831625293562 1104 74-75 0.170022845287221 1104 76-77 0.28158625371814594 1104 78-79 0.3322372653949941 1104 80-81 0.3533179985952728 1104 82-83 0.31753526802689436 1104 84-85 0.2648878098466909 1104 86-87 0.31050635773441826 1104 88-89 0.344428044071865 1104 90-91 0.23491920262624433 1104 92-93 0.21825527771208542 1104 94-95 0.15857790346822753 1104 96-97 0.26113753924153826 1104 98-99 0.2275106311443551 1104 100-101 0.1917101909028105 1104 102-103 0.1639979837501997 1104 104-105 0.20695368611068332 1104 106-107 0.21913192754685085 1104 108-109 0.11146576590186541 1104 110-111 0.21887506390730493 1104 112-113 0.10677316854500063 1104 114-115 0.16874953411502602 1104 116-117 0.1967996029311223 1104 118-119 0.14947095515839592 1104 120-121 0.26055785923626473 1104 122-123 0.15757542591280682 1104 124-125 0.05292161925670413 1105 1 0.2505821248624507 1105 2 0.4247234447296506 1105 3 0.26878646601230827 1105 4 0.14965625990587483 1105 5 0.15168549698037737 1105 6 0.13455370661294097 1105 7 0.05809700666327444 1105 8 0.057919660015343766 1105 9 0.15918879529094454 1105 10-11 0.15771057450319148 1105 12-13 0.24326557824694106 1105 14-15 0.2804660127564702 1105 16-17 0.24573799299936638 1105 18-19 0.225413826895128 1105 20-21 0.3102774963994861 1105 22-23 0.34656322892170266 1105 24-25 0.27834856788680185 1105 26-27 0.22623925283615165 1105 28-29 0.29595314038008524 1105 30-31 0.26558577472362543 1105 32-33 0.2660556409114818 1105 34-35 0.1523239703424295 1105 36-37 0.182794137929136 1105 38-39 0.23682176989990467 1105 40-41 0.23681793039274623 1105 42-43 0.27834105303261225 1105 44-45 0.18924910504927794 1105 46-47 0.20897085271013793 1105 48-49 0.03204380332732626 1105 50-51 0.16077388551590133 1105 52-53 0.10747996074680799 1105 54-55 0.023163974229397866 1105 56-57 -0.153176858063361 1105 58-59 0.04429887105169428 1105 60-61 0.021790631694507567 1105 62-63 0.1747089312828578 1105 64-65 0.21181270010418984 1105 66-67 0.32369008828008816 1105 68-69 0.3994808169077473 1105 70-71 0.3688164689896496 1105 72-73 0.36281023077079766 1105 74-75 0.3770408525761013 1105 76-77 0.46183524560247236 1105 78-79 0.3017902321353816 1105 80-81 0.42623707175566494 1105 82-83 0.3737745414075633 1105 84-85 0.41226685835265187 1105 86-87 0.3034701431751685 1105 88-89 0.33675218721085187 1105 90-91 0.31481897309524953 1105 92-93 0.17827629069297402 1105 94-95 0.16561038702945297 1105 96-97 0.22503580537213708 1105 98-99 0.24705807600766505 1105 100-101 0.1993888833223174 1105 102-103 0.2319276678689839 1105 104-105 0.16881214099992903 1105 106-107 0.1960889852519614 1105 108-109 0.21950561893039122 1105 110-111 0.29745823979251895 1105 112-113 0.3920852739919596 1105 114-115 0.26781708308810437 1105 116-117 0.2508011729350912 1105 118-119 0.26339130879731343 1105 120-121 0.21408947272875167 1105 122-123 0.19815301425537868 1105 124-125 0.06303933970645303 1106 1 0.10062615487523274 1106 2 0.3196768814832538 1106 3 0.05430602016319597 1106 4 0.02846696703993956 1106 5 0.03824910527008285 1106 6 0.02819529024584 1106 7 0.03508076984212494 1106 8 -0.056905788125746426 1106 9 0.16605747728568332 1106 10-11 0.12222087631988643 1106 12-13 0.10140057547244652 1106 14-15 0.12211026231616984 1106 16-17 0.04254521392146415 1106 18-19 0.06429172503501235 1106 20-21 0.06958833622675087 1106 22-23 0.10454261322530556 1106 24-25 0.06864718787763735 1106 26-27 -0.03879940913397917 1106 28-29 -0.08747227398455237 1106 30-31 -0.22592548007142454 1106 32-33 0.03166397886896988 1106 34-35 0.13929349916098488 1106 36-37 -0.02313875550617439 1106 38-39 0.09023138171200173 1106 40-41 0.0777745879719447 1106 42-43 0.06770358288858347 1106 44-45 0.15616507407145264 1106 46-47 0.19401693343585436 1106 48-49 0.12784738121822414 1106 50-51 0.22941818332767383 1106 52-53 -0.10142857745676537 1106 54-55 -0.10485147849563248 1106 56-57 -0.08314983690483047 1106 58-59 -0.037526987231984776 1106 60-61 -0.2027588146130057 1106 62-63 7.584137794154344E-4 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-0.4088102185213387 2216 58-59 -0.47831380329553497 2216 60-61 -0.3928516798962818 2216 62-63 -0.4716892013994922 2216 64-65 -0.6515090695647459 2216 66-67 -0.5461837107405714 2216 68-69 -0.5846798180944646 2216 70-71 -0.5154269675600958 2216 72-73 -0.5293329016973942 2216 74-75 -0.4513550815823919 2216 76-77 -0.6044987872611713 2216 78-79 -0.5996759263010958 2216 80-81 -0.5070855544810016 2216 82-83 -0.48084021725447457 2216 84-85 -0.47912820496198094 2216 86-87 -0.5134706251660859 2216 88-89 -0.5598508079209843 2216 90-91 -0.4380997814552714 2216 92-93 -0.4254340752042083 2216 94-95 -0.4444500009957295 2216 96-97 -0.5930263528930269 2216 98-99 -0.5061062279596698 2216 100-101 -0.5361900850846091 2216 102-103 -0.6022972097132104 2216 104-105 -0.6027640863902484 2216 106-107 -0.5146495188952542 2216 108-109 -0.4054105088124089 2216 110-111 -0.5961660410474892 2216 112-113 -0.47467704273823585 2216 114-115 -0.4797124328083662 2216 116-117 -0.5616033554529913 2216 118-119 -0.581857595509792 2216 120-121 -0.6352566805683217 2216 122-123 -0.615487113087692 2216 124-125 -0.6149147482630539 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56.0 3 9.0 4 25.0 5 112.0 6 310.0 7 620.0 8 1118.0 9 1716.0 10 2792.0 11 3593.0 12 4613.0 13 6520.0 14 9254.0 15 6929.0 16 3523.0 17 3263.0 18 3137.0 19 3399.0 20 3341.0 21 3411.0 22 3759.0 23 4160.0 24 5383.0 25 7320.0 26 9612.0 27 12800.0 28 16662.0 29 21199.0 30 26443.0 31 33005.0 32 41462.0 33 52834.0 34 69847.0 35 100974.0 36 181061.0 37 350557.0 38 158084.0 39 342.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.834873940832345 20.335411765522316 13.312722503278994 23.516991790366344 2 15.30514380060944 21.93845961737574 39.230866619146475 23.525529962868347 3 17.759311704730905 28.246241413218225 30.375312384562957 23.619134497487916 4 12.006202728923043 17.59889829537763 38.445291375534104 31.94960760016522 5 11.756814646134421 40.19873774907256 35.13629292268843 12.908154682104584 6 29.550095599807502 38.857744885085125 18.19205806225043 13.400101452856939 7 26.98839395347869 33.842239627052564 22.66422243650667 16.50514398296207 8 26.56226560704794 34.69228134524754 20.914983373004002 17.83046967470052 9 27.65414114086773 14.711878221886938 20.676959362494525 36.957021274750815 10-11 23.839427008137907 27.458389154082614 28.71874579989508 19.983438037884405 12-13 24.962258670100457 25.267657783038295 28.78443002137447 20.98565352548678 14-15 22.514903598107946 25.938417248719915 26.83224293190085 24.71443622127128 16-17 21.01695650100369 29.395141535406616 27.815468525768594 21.772433437821103 18-19 21.097911154139474 27.71242351470135 30.31919944122864 20.87046588993054 20-21 22.1176332869425 26.685266357105387 30.349405373532946 20.847694982419174 22-23 21.935755195123328 26.84520635250966 30.07452882952018 21.144509622846837 24-25 21.617349305654912 27.138986078413517 30.20862869555038 21.035035920381183 26-27 21.116141378948548 27.41634229098688 30.212462095953924 21.25505423411065 28-29 21.237928585612202 26.80601886335987 30.596596895184963 21.359455655842968 30-31 21.100638201935418 26.91144081023898 30.393890257015016 21.594030730810587 32-33 21.24197373498259 27.07460253458719 29.94060238717705 21.74282134325317 34-35 21.890144765422786 26.83900645569675 30.09286838442829 21.177980394452174 36-37 22.017914666874773 26.40852550845657 29.833730040017514 21.73982978465114 38-39 21.50176241821989 26.932958738169255 29.716885830851208 21.848393012759647 40-41 21.6253900976122 26.456097055505502 30.212566887812304 21.705945959069993 42-43 21.519944469470566 26.474509414566533 29.722793920950508 22.2827521950124 44-45 21.343903507060514 26.767425828856833 29.801429878299928 22.08724078578273 46-47 22.072369704616104 26.89138040919319 28.904309145064577 22.131940741126126 48-49 21.701633217573022 27.060945419230087 28.861256714748386 22.37616464844851 50-51 22.09327683765238 26.990026832991614 28.653507560625695 22.26318876873031 52-53 22.202307402156524 26.884400105788448 28.7010565838135 22.212235908241524 54-55 21.637683232964374 27.2271720232908 28.819851809459397 22.31529293428543 56-57 21.707589793793915 27.304432427089083 28.64760528927426 22.340372489842743 58-59 21.662486424147566 26.521178329796207 29.046666247558516 22.769668998497714 60-61 21.516255911595312 27.49648382559775 28.66133040593388 22.32592985687306 62-63 21.422201180066082 27.114552428569755 29.232107266437485 22.231139124926674 64-65 21.35738719873054 26.90113549159112 28.93769320482638 22.803784104851964 66-67 21.26482230575463 27.208442265086774 28.558285533429583 22.96844989572901 68-69 21.605036223872638 26.832806558883842 28.896244943615624 22.665912273627896 70-71 21.72816704169539 26.723202788652888 28.588547966824045 22.96008220282767 72-73 21.4534639213697 26.909112981196536 28.225528833855773 23.411894263577988 74-75 21.198357677683408 27.367428430212144 28.236367814297914 23.197846077806535 76-77 21.517240482291275 26.844339234074287 28.649983307970118 22.98843697566432 78-79 21.476139068454664 26.8560453329518 28.443652476273474 23.224163122320064 80-81 21.54897701702587 27.2619434725492 27.9277603631492 23.26131914727573 82-83 21.714726705947133 26.83831276094845 28.19756426431504 23.249396268789372 84-85 21.32408985081227 26.837402286591317 28.19184128264159 23.64666657995482 86-87 21.4057290515025 27.084921243968108 28.117312453121407 23.39203725140798 88-89 21.47809008493425 26.920125385325754 28.014125359312203 23.587659170427795 90-91 21.259473823177846 26.920463891623932 28.220753032642282 23.599309252555944 92-93 21.32368402683388 26.785886935413032 28.14596397031503 23.744465067438057 94-95 21.22210361927819 26.34748504432291 28.37130313611812 24.059108200280775 96-97 21.47471875402407 26.162737005583946 28.399350859097144 23.96319338129484 98-99 21.13824592865725 26.513688791108542 28.177173597744964 24.17089168248925 100-101 20.999091537859453 26.23825124290196 28.385647599079828 24.377009620158756 102-103 21.0412872678079 25.89056836117037 28.42824808782975 24.63989628319198 104-105 20.72216441207076 26.246704821366635 28.195802983003816 24.835327783558792 106-107 20.952922342591727 26.09325514518921 28.0820403151114 24.871782197107663 108-109 20.992984560308976 26.13026889700325 27.762825324267332 25.113921218420437 110-111 20.88415143081245 26.181576319608453 28.000426648721984 24.93384560085711 112-113 20.675263366240472 26.213054259277165 28.37977461737807 24.73190775710429 114-115 20.65648984702686 26.64291555130076 27.764741690361415 24.93585291131096 116-117 20.32356524415887 26.556195778000337 28.067669922696393 25.052569055144396 118-119 20.20397518791809 26.55909041443218 27.903576784652824 25.333357612996902 120-121 20.07741660242742 26.769729830972018 27.911251854555985 25.241601712044577 122-123 20.019007334993006 27.038714438204046 27.74628676276123 25.19599146404171 124-125 19.98799514601424 26.871139515659653 27.747649989114098 25.393215349212007 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 220.0 1 164.0 2 261.5 3 416.0 4 271.0 5 189.5 6 420.5 7 993.0 8 1655.0 9 1789.5 10 1247.0 11 692.0 12 544.0 13 552.5 14 595.0 15 643.5 16 723.0 17 834.0 18 909.0 19 983.0 20 1141.0 21 1350.5 22 1588.0 23 1933.0 24 2322.0 25 2890.0 26 3948.5 27 5410.5 28 6945.5 29 9048.5 30 12317.0 31 15823.5 32 21240.0 33 26156.5 34 29296.0 35 32359.5 36 37077.0 37 41684.0 38 43439.5 39 45250.5 40 44488.5 41 42903.0 42 42131.0 43 42194.0 44 43007.5 45 43861.5 46 43255.5 47 41719.5 48 39986.5 49 37555.0 50 37115.5 51 36101.0 52 34626.5 53 34383.5 54 34599.5 55 32099.5 56 28103.5 57 25115.0 58 23299.5 59 20832.5 60 16285.5 61 13201.5 62 11128.0 63 8291.0 64 6002.0 65 4398.5 66 2966.0 67 2232.5 68 1692.0 69 1218.0 70 876.5 71 595.0 72 471.5 73 381.0 74 303.0 75 249.5 76 144.5 77 84.0 78 79.0 79 40.0 80 9.0 81 3.0 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03928046512232873 2 0.004422304020394625 3 0.0016475250272058408 4 0.07361835516303994 5 0.005809693516989018 6 0.0 7 0.0045957277074689246 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.335592176857476E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.4684737414859807E-4 28-29 3.4684737414859807E-4 30-31 4.335592176857476E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.936947482971961E-4 42-43 4.769151394543224E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.335592176857476E-5 52-53 0.0 54-55 0.0 56-57 4.769151394543224E-4 58-59 2.167796088428738E-4 60-61 2.6013553061144854E-4 62-63 3.902032959171728E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 1.3006776530572427E-4 92-93 1.3006776530572427E-4 94-95 3.4684737414859807E-4 96-97 0.0024712875408087615 98-99 0.00810755737072348 100-101 0.01712558909858703 102-103 0.0091047435714007 104-105 0.0039020329591717283 106-107 0.011185827816292288 108-109 0.012616573234655256 110-111 0.00619989681290619 112-113 0.004378948098626051 114-115 0.0031216263673373825 116-117 0.0 118-119 0.001430745418362967 120-121 3.4684737414859807E-4 122-123 5.202710612228971E-4 124-125 0.03277707685704252 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1153245.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.546908079888574 #Duplication Level Percentage of deduplicated Percentage of total 1 73.24271123691295 26.035519238603666 2 11.29838953006048 8.032456281516707 3 4.678364285718198 4.989041556859751 4 2.5730070763376958 3.658497841259157 5 1.6427385807023658 2.919713866375681 6 1.092289965968865 2.329651861012792 7 0.7906127454415832 1.9672687012300214 8 0.5995767113418347 1.7050478595928065 9 0.42392962591685346 1.3562448700327143 >10 3.0729010577018947 21.164148636910504 >50 0.3591246332749734 8.96275066187082 >100 0.21759541322552178 13.74229440227255 >500 0.006915108471150057 1.5492630639416722 >1k 0.0018440289256400153 1.588101158521128 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3523 0.30548582478137776 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2953 0.25606007396520253 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2081 0.18044734640080815 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.671184353714952E-5 0.0 18-19 0.0 0.0 0.0 8.671184353714952E-5 0.0 20-21 0.0 0.0 0.0 8.671184353714952E-5 0.0 22-23 0.0 0.0 0.0 2.167796088428738E-4 0.0 24-25 0.0 0.0 0.0 0.0012573217312886681 0.0 26-27 0.0 0.0 0.0 0.0031216263673373825 0.0 28-29 0.0 0.0 0.0 0.007717354074806308 0.0 30-31 0.0 0.0 0.0 0.023542265520336093 0.0 32-33 0.0 0.0 0.0 0.06052486678893036 0.0 34-35 0.0 0.0 0.0 0.11550017559148316 0.0 36-37 0.0 0.0 0.0 0.19596876639395794 0.0 38-39 0.0 0.0 0.0 0.325039345499005 0.0 40-41 0.0 0.0 0.0 0.4672034129781616 0.0 42-43 0.0 0.0 0.0 0.6055088034199152 0.0 44-45 0.0 0.0 0.0 0.7729927292119194 0.0 46-47 0.0 0.0 0.0 0.970522308789546 0.0 48-49 0.0 0.0 0.0 1.1872585617106513 0.0 50-51 0.0 0.0 0.0 1.423028064288161 0.0 52-53 0.0 0.0 0.0 1.6526843818963015 0.0 54-55 0.0 0.0 0.0 1.8962145944703859 0.0 56-57 0.0 0.0 0.0 2.1756435102688503 0.0 58-59 0.0 0.0 0.0 2.4857684186794655 0.0 60-61 0.0 0.0 0.0 2.8319654540015344 0.0 62-63 0.0 0.0 0.0 3.173696829381441 0.0 64-65 0.0 0.0 0.0 3.5162519672749504 0.0 66-67 0.0 0.0 0.0 3.8966134689506564 0.0 68-69 0.0 0.0 0.0 4.3331208893166675 0.0 70-71 0.0 0.0 0.0 4.7992404042506145 0.0 72-73 0.0 0.0 0.0 5.27242693443284 0.0 74-75 0.0 0.0 0.0 5.730352180152526 0.0 76-77 0.0 0.0 0.0 6.210605725583029 0.0 78-79 0.0 0.0 0.0 6.7683363032139745 0.0 80-81 0.0 0.0 0.0 7.382906494283522 0.0 82-83 0.0 0.0 0.0 8.029429999696509 0.0 84-85 0.0 0.0 0.0 8.65362520539868 0.0 86-87 0.0 0.0 0.0 9.303703896396689 0.0 88-89 0.0 0.0 0.0 9.984218444476237 0.0 90-91 0.0 0.0 0.0 10.74108277079025 0.0 92-93 0.0 0.0 0.0 11.516720211230052 0.0 94-95 0.0 0.0 0.0 12.299207887309288 0.0 96-97 0.0 0.0 0.0 13.094355492544949 0.0 98-99 0.0 0.0 0.0 13.910964279056056 0.0 100-101 0.0 0.0 0.0 14.800411014138366 0.0 102-103 0.0 0.0 0.0 15.75155322589736 0.0 104-105 0.0 0.0 0.0 16.69705916782644 0.0 106-107 0.0 0.0 0.0 17.61412362507533 0.0 108-109 0.0 0.0 0.0 18.56071346504862 0.0 110-111 0.0 0.0 0.0 19.562712173042154 0.0 112-113 0.0 0.0 0.0 20.60776331135188 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1950 0.0 84.011826 1 GTATCAA 4295 0.0 60.196182 1 ATCAACG 5460 0.0 46.79034 3 TCAACGC 5510 0.0 46.365746 4 CAACGCA 5630 0.0 45.445786 5 AACGCAG 5915 0.0 43.35481 6 TATCAAC 5895 0.0 43.240353 2 GTACATG 7855 0.0 39.133247 1 TACATGG 8335 0.0 36.72711 2 ACGCAGA 7000 0.0 36.634815 7 CGCAGAG 7115 0.0 35.95594 8 CATGGGA 6285 0.0 35.72133 4 ACATGGG 8460 0.0 35.61818 3 CCGTTGC 90 2.3942874E-4 33.052525 9 GCAGAGT 8105 0.0 31.050203 9 ATGGGAT 2680 0.0 30.861256 5 CATATAG 430 0.0 30.46813 3 TGGTATC 885 0.0 28.264105 2 ATGGGAG 2340 0.0 28.22544 5 GGAACGC 235 3.4197E-10 27.848507 8 >>END_MODULE