FastQCFastQC Report
Thu 26 May 2016
SRR1364439_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364439_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019355
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30300.054.032911
ATCAACG34550.045.4503063
CAACGCA35150.045.016275
TCAACGC35250.044.7164884
AACGCAG37650.042.339996
TATCAAC39550.040.606752
GTACATG91450.038.865021
GGTATCA19000.038.2273751
TACATGG96350.035.8061182
ACATGGG95700.034.619773
ACGCAGA47050.033.6281477
CGCAGAG47700.033.0452048
CATGGGG49200.029.6197874
CATGGGA54150.028.9992264
GCAGAGT60600.024.832989
ATGGGGA27800.022.681635
GAGTACA71600.020.8703141
ATGGGAG26450.020.6907085
AGAGTAC56900.020.64599210-11
TCGCGCA1450.003875398320.5108179
Produced by FastQC (version 0.11.5)