FastQCFastQC Report
Thu 26 May 2016
SRR1364439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364439_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019355
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.11434344134637049No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21450.1062220362442463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30650.062.980281
GTATCAA50100.053.2758451
ATCAACG64800.040.7082023
TCAACGC66100.039.907594
CAACGCA65950.039.8794065
TATCAAC66350.039.7621422
AACGCAG68550.038.2743456
GTACATG84850.034.195361
ACGCAGA80950.032.484947
TACATGG88500.032.3674772
ACATGGG88300.032.032153
CGCAGAG82350.032.0049258
GTGGTAT15800.029.4122051
CATGGGA54150.027.1613734
GCAGAGT96850.026.7218369
CATGGGG44600.024.4325854
ATGGGAT25400.024.3635645
GAGTACT48400.024.27719112-13
GTAAGGT8400.024.1019864
TAAGGTG10400.024.0301865
Produced by FastQC (version 0.11.5)