FastQCFastQC Report
Thu 26 May 2016
SRR1364438_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364438_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836389
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17000.055.3245961
GTATCAA28950.048.320271
ATCAACG31350.042.874833
TCAACGC31900.042.3255044
CAACGCA32250.041.869585
GTACATG95100.040.5606161
TACATGG99000.038.087772
TATCAAC36800.036.8483922
AACGCAG36600.036.7297486
ACATGGG98500.036.590463
CATGGGA74450.035.631754
CGCAGAG44450.030.243178
ACGCAGA44750.029.90757
ATGGGAT33400.027.2488045
GAGTACA64900.026.4154421
ATGGGAG33650.025.4553995
CATGGGG27400.023.6615354
GCGTTAG1552.1005953E-423.0425011
AGTACAT71450.022.8076132
TAGCCCT15550.022.5677664
Produced by FastQC (version 0.11.5)