##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364438_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1836389 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.750403645415 31.0 31.0 34.0 27.0 34.0 2 30.812452045835606 31.0 31.0 34.0 27.0 34.0 3 30.741923416008262 31.0 31.0 34.0 27.0 34.0 4 34.337259698244765 37.0 35.0 37.0 30.0 37.0 5 34.51332479120709 37.0 35.0 37.0 31.0 37.0 6 34.620825979680774 37.0 35.0 37.0 31.0 37.0 7 34.507711056862135 37.0 35.0 37.0 31.0 37.0 8 34.35520578700918 37.0 35.0 37.0 30.0 37.0 9 35.458733416503804 39.0 35.0 39.0 29.0 39.0 10-11 35.69345628840077 39.0 35.0 39.0 30.0 39.0 12-13 35.738097429248384 39.0 35.0 39.0 30.0 39.0 14-15 36.88510876508191 40.0 37.0 41.0 30.0 41.0 16-17 37.03483793466417 40.0 37.0 41.0 31.0 41.0 18-19 37.03700822647053 40.0 37.0 41.0 30.0 41.0 20-21 36.994764181227396 40.0 37.0 41.0 30.0 41.0 22-23 36.91116370224391 40.0 37.0 41.0 30.0 41.0 24-25 36.96677773608968 40.0 37.0 41.0 30.0 41.0 26-27 36.98280102962934 40.0 37.0 41.0 30.0 41.0 28-29 36.936540133925874 40.0 36.5 41.0 30.0 41.0 30-31 36.707418744067844 40.0 36.0 41.0 30.0 41.0 32-33 36.784112462011045 40.0 36.0 41.0 30.0 41.0 34-35 36.74145129381629 40.0 36.0 41.0 30.0 41.0 36-37 36.6228269173906 40.0 36.0 41.0 30.0 41.0 38-39 36.489601059470516 40.0 36.0 41.0 29.5 41.0 40-41 36.47321400857879 40.0 36.0 41.0 30.0 41.0 42-43 36.34941126308206 40.0 35.0 41.0 29.0 41.0 44-45 36.345438520923395 40.0 35.0 41.0 29.0 41.0 46-47 36.2189786586611 39.5 35.0 41.0 29.0 41.0 48-49 36.06028297925984 39.0 35.0 41.0 28.5 41.0 50-51 35.9483859356596 39.0 35.0 41.0 28.5 41.0 52-53 35.83500690757786 39.0 35.0 41.0 28.0 41.0 54-55 35.72768895914754 39.0 35.0 40.5 28.0 41.0 56-57 35.696726292740806 39.0 35.0 40.5 28.0 41.0 58-59 35.689573124212785 39.0 35.0 41.0 28.0 41.0 60-61 35.51959606597513 39.0 35.0 41.0 28.0 41.0 62-63 35.27764896217523 38.0 34.0 40.0 28.0 41.0 64-65 34.98655295800617 37.5 34.0 40.0 28.0 41.0 66-67 34.70018389349969 37.0 34.0 40.0 28.0 41.0 68-69 34.40355774294008 37.0 34.0 39.5 27.0 41.0 70-71 34.05733017350899 36.0 33.5 39.0 26.0 41.0 72-73 33.72592326571331 36.0 33.0 39.0 26.0 40.5 74-75 33.35876113394275 35.0 33.0 38.5 26.0 40.0 76-77 33.00296641942421 35.0 33.0 37.0 26.0 39.0 78-79 32.66455309849928 35.0 33.0 37.0 26.0 39.0 80-81 32.32265957811771 35.0 32.5 36.0 25.5 39.0 82-83 32.00509614248398 35.0 32.0 36.0 25.0 37.0 84-85 31.635143752222433 35.0 32.0 35.5 24.0 37.0 86-87 31.427072913200853 35.0 32.0 35.0 24.0 36.5 88-89 31.264025214701242 35.0 32.0 35.0 24.0 36.0 90-91 31.11148209883636 35.0 32.0 35.0 23.0 36.0 92-93 30.87180439438485 35.0 32.0 35.0 21.0 36.0 94-95 30.768149068634152 35.0 31.0 35.0 20.5 35.5 96-97 30.673795149067 35.0 31.0 35.0 20.0 35.0 98-99 30.513597336947672 34.0 31.0 35.0 19.5 35.0 100-101 30.372929156077497 34.0 31.0 35.0 18.5 35.0 102-103 29.333248837800703 33.5 29.5 34.5 17.5 35.0 104-105 30.25922530574949 34.0 31.0 35.0 19.0 35.0 106-107 30.481405628110384 35.0 32.0 35.0 19.0 35.0 108-109 30.46542617059893 35.0 32.0 35.0 18.0 35.0 110-111 30.33458052732836 35.0 32.0 35.0 16.0 35.0 112-113 30.19939130543692 35.0 31.5 35.0 11.0 35.0 114-115 30.078861559288363 35.0 31.0 35.0 4.5 35.0 116-117 29.938004420632012 35.0 31.0 35.0 2.0 35.0 118-119 29.66434644293774 35.0 31.0 35.0 2.0 35.0 120-121 29.503959128485306 35.0 31.0 35.0 2.0 35.0 122-123 29.256862788875342 34.5 31.0 35.0 2.0 35.0 124-125 28.066150744749613 33.5 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7955412060644029 1101 2 0.8028704987254756 1101 3 0.9060850366193911 1101 4 0.8472863156735286 1101 5 0.6707556136032125 1101 6 0.7512853851459198 1101 7 0.7804510454974292 1101 8 0.840567958266007 1101 9 1.3046149128774687 1101 10-11 0.9828544525703506 1101 12-13 0.8689011775166762 1101 14-15 1.0997452118583624 1101 16-17 0.8356543019231992 1101 18-19 0.9712811145552607 1101 20-21 1.0609105678311082 1101 22-23 0.9557645117735234 1101 24-25 1.0478438105066346 1101 26-27 0.9953532079474243 1101 28-29 0.987919718451117 1101 30-31 1.0319484581676122 1101 32-33 0.9876147745872288 1101 34-35 0.9201086882041736 1101 36-37 0.8419693894574252 1101 38-39 0.9616355469809861 1101 40-41 1.0001065852956614 1101 42-43 1.0108904182112326 1101 44-45 0.9662337564590189 1101 46-47 0.9538991639107053 1101 48-49 0.8826663004760533 1101 50-51 0.8873408122622237 1101 52-53 0.7036567548407078 1101 54-55 0.6328179327312995 1101 56-57 0.7434984594962231 1101 58-59 0.817336421027349 1101 60-61 0.7780711745989564 1101 62-63 0.8140446118538236 1101 64-65 0.659750686731762 1101 66-67 0.6037143126176403 1101 68-69 0.5638082780249078 1101 70-71 0.6697733059488229 1101 72-73 0.7326931338010851 1101 74-75 0.7014676729047196 1101 76-77 0.6836631922883285 1101 78-79 0.691727390550227 1101 80-81 0.6314427667898528 1101 82-83 0.7020978026285221 1101 84-85 0.7303353403041903 1101 86-87 0.7476824343137096 1101 88-89 0.69367792105459 1101 90-91 0.722577127456244 1101 92-93 0.7587350360874225 1101 94-95 0.7847235902007128 1101 96-97 0.77064009791318 1101 98-99 0.7275381467419564 1101 100-101 0.7590458837692751 1101 102-103 0.7604132413439295 1101 104-105 0.7595503657162652 1101 106-107 0.6231892628054609 1101 108-109 0.6647220363620541 1101 110-111 0.6467861687490846 1101 112-113 0.5715687869192578 1101 114-115 0.6417694249144716 1101 116-117 0.6855380555373323 1101 118-119 0.47810010400905156 1101 120-121 0.233349843597221 1101 122-123 0.30990279321526515 1101 124-125 0.07630356099352298 1102 1 0.27519799147983903 1102 2 0.2617711695165674 1102 3 0.3957735097122992 1102 4 0.3595292819693583 1102 5 0.2573672269312439 1102 6 0.1148047749866592 1102 7 0.3548992546635361 1102 8 0.39274974402438545 1102 9 0.7213407938909242 1102 10-11 0.41618892903779425 1102 12-13 0.4207071007803762 1102 14-15 0.5351736813846983 1102 16-17 0.3908340711930123 1102 18-19 0.32399102827150017 1102 20-21 0.569256361326218 1102 22-23 0.637089358479237 1102 24-25 0.5417957165684442 1102 26-27 0.5003429883285051 1102 28-29 0.3179338475886908 1102 30-31 0.4377818715203361 1102 32-33 0.39763823169912627 1102 34-35 0.344672125288362 1102 36-37 0.4054599723186456 1102 38-39 0.46460060823010707 1102 40-41 0.42020425467384115 1102 42-43 0.42770477780463523 1102 44-45 0.2953336069370778 1102 46-47 0.3864078402985882 1102 48-49 0.21815512748328558 1102 50-51 0.1318455379786272 1102 52-53 0.18072502738216656 1102 54-55 0.0940272771667452 1102 56-57 0.22524923797055862 1102 58-59 0.2383463114236477 1102 60-61 0.23889093893042457 1102 62-63 0.26049586632918675 1102 64-65 0.32678178455203266 1102 66-67 0.12722560906889413 1102 68-69 0.18671325614531753 1102 70-71 0.24402055213876395 1102 72-73 0.22163468306787593 1102 74-75 0.22027314348730442 1102 76-77 0.214149207185649 1102 78-79 0.19954437020701477 1102 80-81 0.2133142602259781 1102 82-83 0.13970119910858614 1102 84-85 0.2575819417664853 1102 86-87 0.21193434449079973 1102 88-89 0.09225471909022787 1102 90-91 0.048877213877180026 1102 92-93 0.055376965331994654 1102 94-95 0.14076894061704692 1102 96-97 0.09484481951430013 1102 98-99 0.0795436612208924 1102 100-101 -0.05424510787669945 1102 102-103 0.029878528933611648 1102 104-105 0.05424302268000858 1102 106-107 0.01765626509629925 1102 108-109 0.0461977767700148 1102 110-111 -0.03662306868901766 1102 112-113 -0.13831406469736862 1102 114-115 -0.23395520779654078 1102 116-117 -0.27456084842568274 1102 118-119 -0.1293986271120886 1102 120-121 -0.16717897024109973 1102 122-123 -0.18497779499582379 1102 124-125 -0.40300685478721476 1103 1 0.5094618864270721 1103 2 0.5642679305631901 1103 3 0.6363304143796498 1103 4 0.6148805537875361 1103 5 0.5355916945384536 1103 6 0.44724443930739 1103 7 0.5312649862112693 1103 8 0.6035538508219531 1103 9 1.0566476643783602 1103 10-11 0.679796439503356 1103 12-13 0.6697435528406075 1103 14-15 0.7940731533864707 1103 16-17 0.8291679796168978 1103 18-19 0.7372267915251598 1103 20-21 0.8295572096390416 1103 22-23 0.7656461850210761 1103 24-25 0.6444122700616361 1103 26-27 0.8135885172707518 1103 28-29 0.7468161970831559 1103 30-31 0.9480111047835749 1103 32-33 0.8255368508307583 1103 34-35 0.6735505685073946 1103 36-37 0.7157084114360828 1103 38-39 0.7850327073974199 1103 40-41 0.7710506143506635 1103 42-43 0.73994574291509 1103 44-45 0.6912268093667748 1103 46-47 0.5838026801087395 1103 48-49 0.568613463800574 1103 50-51 0.5916040650523726 1103 52-53 0.5483068475763133 1103 54-55 0.5247670606288821 1103 56-57 0.5421956373611749 1103 58-59 0.6302101907457782 1103 60-61 0.6497393168495549 1103 62-63 0.644941077561036 1103 64-65 0.7054515675584341 1103 66-67 0.5646936264387961 1103 68-69 0.6011710325631299 1103 70-71 0.6750686058370263 1103 72-73 0.5277167539146888 1103 74-75 0.553130128233299 1103 76-77 0.5416129276525723 1103 78-79 0.48066761970733296 1103 80-81 0.4610141150427438 1103 82-83 0.42583327028091844 1103 84-85 0.5315606135026307 1103 86-87 0.5775168027099298 1103 88-89 0.3931108444556273 1103 90-91 0.48368310454053187 1103 92-93 0.4245811919220088 1103 94-95 0.3783665161220924 1103 96-97 0.4520577570985296 1103 98-99 0.5035057312864524 1103 100-101 0.44817287661538785 1103 102-103 0.3677261060662751 1103 104-105 0.33687311792802177 1103 106-107 0.23475924102713464 1103 108-109 0.17313512662255448 1103 110-111 0.27255277229953023 1103 112-113 0.32187187401317274 1103 114-115 0.3136414562442411 1103 116-117 0.18499650546334223 1103 118-119 0.1153571981211492 1103 120-121 0.17194465686584692 1103 122-123 0.26792684631043784 1103 124-125 0.12871210171640612 1104 1 0.5395080899345537 1104 2 0.5997674764479868 1104 3 0.5996593662357732 1104 4 0.3781784648575979 1104 5 0.3992835553377745 1104 6 0.3488100190355823 1104 7 0.4077212525539906 1104 8 0.5156261618020892 1104 9 1.0425477385952178 1104 10-11 0.7171098914048315 1104 12-13 0.6507127407088973 1104 14-15 0.6354913340898278 1104 16-17 0.5979833584741598 1104 18-19 0.6491573112603319 1104 20-21 0.6893495557220817 1104 22-23 0.8281488515906616 1104 24-25 0.8619689003352136 1104 26-27 0.801561809420754 1104 28-29 0.7625437841666098 1104 30-31 0.8214061492434368 1104 32-33 0.7932161752401896 1104 34-35 0.8086130524600748 1104 36-37 0.6677668115454338 1104 38-39 0.7580755091924232 1104 40-41 0.8100626870448693 1104 42-43 0.6712226561069059 1104 44-45 0.4790544142060753 1104 46-47 0.4723829920750262 1104 48-49 0.4551885793093504 1104 50-51 0.5065792004885523 1104 52-53 0.4350200498740975 1104 54-55 0.33021216378520535 1104 56-57 0.36711065841909374 1104 58-59 0.3773923771667782 1104 60-61 0.4819120696818686 1104 62-63 0.5334369534796437 1104 64-65 0.5097500138585076 1104 66-67 0.3054675511565961 1104 68-69 0.30280060996135205 1104 70-71 0.38991913103946274 1104 72-73 0.4407148595325623 1104 74-75 0.46677082921856083 1104 76-77 0.4772766831278332 1104 78-79 0.3434253189080678 1104 80-81 0.41587934310310004 1104 82-83 0.41309524301867384 1104 84-85 0.3720314115318608 1104 86-87 0.4396444250495861 1104 88-89 0.43985788504241086 1104 90-91 0.359121411936286 1104 92-93 0.3955888422622458 1104 94-95 0.33468012258879654 1104 96-97 0.25383589595431033 1104 98-99 0.3414580102294309 1104 100-101 0.31586733819826307 1104 102-103 0.29696371625787066 1104 104-105 0.3245915051867385 1104 106-107 0.30127606883763747 1104 108-109 0.18967769401491097 1104 110-111 0.2550259054134081 1104 112-113 0.23843085597758318 1104 114-115 0.23853048224057716 1104 116-117 0.11532130960619824 1104 118-119 0.016456974448654904 1104 120-121 0.007192299746073161 1104 122-123 0.09971535764287864 1104 124-125 -0.1656905930836885 1105 1 0.39382795622750066 1105 2 0.4801712277076078 1105 3 0.49176248458883975 1105 4 0.3529134285484332 1105 5 0.40755232119293083 1105 6 0.5207506521256278 1105 7 0.5929530761781194 1105 8 0.7311324488165738 1105 9 1.1800363274689687 1105 10-11 0.7289445864276658 1105 12-13 0.7529262316036949 1105 14-15 0.44625128069264264 1105 16-17 0.496452623742897 1105 18-19 0.6790024716430381 1105 20-21 0.9270805880218802 1105 22-23 0.8629954111265405 1105 24-25 0.7876925989316277 1105 26-27 0.9340635258682184 1105 28-29 0.8011940524523169 1105 30-31 0.9245206794873155 1105 32-33 0.7617868370625729 1105 34-35 0.7090063444763359 1105 36-37 0.8275034649239643 1105 38-39 0.8644944875200764 1105 40-41 0.818248879926152 1105 42-43 0.7761759641484502 1105 44-45 0.6760460055249027 1105 46-47 0.7461747124523583 1105 48-49 0.6290207451525731 1105 50-51 0.6342469574243026 1105 52-53 0.5874849296004427 1105 54-55 0.5515237896011911 1105 56-57 0.6533471963483777 1105 58-59 0.7336794681056205 1105 60-61 0.6763244024142523 1105 62-63 0.6375423594099132 1105 64-65 0.7066815868583731 1105 66-67 0.5457950447457165 1105 68-69 0.6506813134219271 1105 70-71 0.6480365131091972 1105 72-73 0.6645166721622573 1105 74-75 0.7248572464230989 1105 76-77 0.6097791641398658 1105 78-79 0.4652388049096601 1105 80-81 0.45045715819162524 1105 82-83 0.45686235970805456 1105 84-85 0.629102104701385 1105 86-87 0.49535327580199606 1105 88-89 0.4721530925987558 1105 90-91 0.47848943815167644 1105 92-93 0.40005313480299876 1105 94-95 0.4466414295048935 1105 96-97 0.3959925338274779 1105 98-99 0.5081109147337841 1105 100-101 0.34388901207477574 1105 102-103 0.2889078816362449 1105 104-105 0.2768471235895049 1105 106-107 0.2467450298096736 1105 108-109 0.21780029041503468 1105 110-111 0.3387912177303889 1105 112-113 0.1818221127229549 1105 114-115 0.13723217513945585 1105 116-117 0.18552477553032176 1105 118-119 0.011714380403390123 1105 120-121 0.004108428541563569 1105 122-123 0.06096454911584104 1105 124-125 -0.0044014913400189926 1106 1 0.3690057012048804 1106 2 0.38582318854142983 1106 3 0.5134602851826138 1106 4 0.3009858760689923 1106 5 0.27500360033634763 1106 6 0.1920637213532288 1106 7 0.23901825470703386 1106 8 0.4466188061110259 1106 9 0.925837323312173 1106 10-11 0.3426743188350869 1106 12-13 0.501692729795586 1106 14-15 0.7809340234023594 1106 16-17 0.5480599875767567 1106 18-19 0.5099119661080991 1106 20-21 0.6879884039914472 1106 22-23 0.636739870047407 1106 24-25 0.5914913853168642 1106 26-27 0.6418636723638471 1106 28-29 0.6052175600461922 1106 30-31 0.6501933161151641 1106 32-33 0.6142705294448518 1106 34-35 0.5496539984542466 1106 36-37 0.5954316243550721 1106 38-39 0.7789566259040868 1106 40-41 0.7433568398904313 1106 42-43 0.6561897480111298 1106 44-45 0.4325344246274341 1106 46-47 0.5548304347512811 1106 48-49 0.5515388202404807 1106 50-51 0.5407360017543752 1106 52-53 0.4758956396521299 1106 54-55 0.3704295163488567 1106 56-57 0.4726058159517663 1106 58-59 0.6345894387054969 1106 60-61 0.6214192037433861 1106 62-63 0.6770700865683068 1106 64-65 0.5818175137317638 1106 66-67 0.5111111477697534 1106 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-1.4058519783339705 2216 74-75 -1.2447722655309406 2216 76-77 -1.1914143606245133 2216 78-79 -1.2201162178469929 2216 80-81 -1.1936798361201113 2216 82-83 -1.418350474716945 2216 84-85 -1.5273701211416189 2216 86-87 -1.4187785000495232 2216 88-89 -1.786663072769855 2216 90-91 -1.50976506610818 2216 92-93 -1.2136005612970493 2216 94-95 -1.2561933983085503 2216 96-97 -1.1946099833441082 2216 98-99 -1.168822721659474 2216 100-101 -1.269410299432451 2216 102-103 -1.658509908379795 2216 104-105 -1.4339954155964705 2216 106-107 -1.1230694365043306 2216 108-109 -1.13139458218755 2216 110-111 -1.036484867329154 2216 112-113 -1.2837731628733025 2216 114-115 -1.1554242112058297 2216 116-117 -1.119047962473104 2216 118-119 -0.8711249643426662 2216 120-121 -0.8822474930756279 2216 122-123 -0.8063459162277908 2216 124-125 -0.7381812677925907 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25052.0 3 5206.0 4 3430.0 5 3085.0 6 3345.0 7 3978.0 8 4290.0 9 4752.0 10 5296.0 11 5869.0 12 6322.0 13 6567.0 14 6711.0 15 6583.0 16 6387.0 17 5854.0 18 5769.0 19 5638.0 20 5759.0 21 6165.0 22 7378.0 23 9496.0 24 13632.0 25 18752.0 26 22612.0 27 24572.0 28 29093.0 29 35733.0 30 42780.0 31 51796.0 32 66038.0 33 86174.0 34 116283.0 35 168049.0 36 294128.0 37 505196.0 38 218133.0 39 486.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.03529616952626 19.275927096731998 12.599522553358478 24.089254180383264 2 15.12096916918476 20.826714775887524 40.27588017057499 23.776435884352722 3 18.285133642781375 26.440272970635835 29.592929163118033 25.681664223464757 4 11.877356245538007 16.763643829066794 38.92028885671141 32.43871106868379 5 12.547260587651337 39.81777003541242 34.95750563206254 12.677463744873693 6 30.93156885317139 37.96048325352077 18.24747084763225 12.860477045675584 7 27.898990900076182 33.15724500636848 22.005577249700366 16.93818684385498 8 25.792804356006254 35.96825460789027 20.66336629889221 17.575574737211255 9 26.58703881993666 16.225544224058687 20.331783904821236 36.85563305118342 10-11 23.890718905696843 27.604011653411487 28.755792191538998 19.74947724935267 12-13 24.731825433107677 25.03304788492175 28.417804770930168 21.817321911040402 14-15 22.105012081339517 26.446343550056827 25.5775599124981 25.871084456105553 16-17 21.929777748250462 29.633956874022854 26.633942170871315 21.802323206855373 18-19 22.049462035890052 28.692925985037597 27.72322630873375 21.534385670338597 20-21 22.79494562167105 27.90947956002803 27.627627791183514 21.667947027117407 22-23 22.527341763052213 28.010916010330018 27.424569845801276 22.03717238081649 24-25 22.15071542199632 28.102118028155054 27.658781312816927 22.088385237031698 26-27 21.89554176619297 28.33218421978458 27.330385938771308 22.441888075251143 28-29 22.110792494095936 28.339245341485526 27.412893070029998 22.137069094388544 30-31 22.21987115229024 28.032750858027057 27.351884636720207 22.395493352962497 32-33 21.86306988526941 28.16104050136535 27.380958539664785 22.594931073700455 34-35 22.592298633848166 28.06834162644903 27.314596110517396 22.024763629185415 36-37 22.655498262605978 27.643896576454324 27.207437930570677 22.493167230369025 38-39 22.223589645291668 28.0689148554059 27.26194767755293 22.445547821749507 40-41 22.45702349004984 27.700435428501724 27.59419218101976 22.248348900428677 42-43 22.16466185599235 27.57952942086212 27.45365355847768 22.802155164667845 44-45 22.10333114059927 27.968087006525927 27.56552608191822 22.36305577095658 46-47 22.60772894587734 28.09438145588833 26.861260327175035 22.436629271059296 48-49 22.13865985557286 27.98804064872401 27.20316737645816 22.670132119244972 50-51 22.4132320967384 27.879245817319408 27.313486277376835 22.394035808565356 52-53 22.504073559332372 27.78456099229291 27.27166283998972 22.439702608384998 54-55 22.068782044422225 27.92711986387256 27.46979461344648 22.53430347825874 56-57 22.232006949153003 28.161524238983333 27.197056957458223 22.40941185440544 58-59 22.026854559450655 27.659036245295717 27.495711989465338 22.81839720578829 60-61 21.834915147346425 27.93912698244129 27.586466054854007 22.639491815358273 62-63 22.037933598910097 27.65613754367543 27.864183871562435 22.441744985852033 64-65 21.898709601351257 27.73236583614505 27.527732992124292 22.8411915703794 66-67 21.829187694856536 27.965883614228105 27.304110038170027 22.90081865274533 68-69 22.207293524905978 27.479861916508884 27.673257229654347 22.63958732893079 70-71 22.096452806926113 27.378359818700353 27.435049994635946 23.09013737973759 72-73 21.849543157105323 27.583818069972544 27.40213203335594 23.164506739566193 74-75 21.838652272241443 27.697776978995197 27.358475922832152 23.105094825931204 76-77 21.987619481209755 27.531133731821868 27.588804926503265 22.892441860465116 78-79 22.124649008728763 27.400600579013062 27.53759337075788 22.937157041500292 80-81 22.06795120053003 27.57664028050355 27.29637636622531 23.05903215274111 82-83 22.11363494012979 27.54498095100637 27.480195310053347 22.861188798810495 84-85 21.963665476211546 27.63419851236226 27.44363492286096 22.95850108856523 86-87 22.056052675365105 27.431727381729466 27.5702837000528 22.941936242852627 88-89 21.989219977435642 27.462370270600733 27.432987043331135 23.115422708632487 90-91 21.961702683861972 27.490169701986755 27.518761981526662 23.029365632624607 92-93 22.01381116929302 27.209419478867986 27.603522501695082 23.17324685014391 94-95 21.83258978168336 26.990244152155064 27.88198876830379 23.295177297857787 96-97 22.063565152252423 26.815798079795318 27.792265647103097 23.328371120849162 98-99 21.865719089727094 26.942253626592063 27.696258024111476 23.49576925956937 100-101 21.73609957602311 26.821779940474404 27.955734307817565 23.486386175684913 102-103 21.589797576671213 26.79658328940088 27.9122316670951 23.70138746683281 104-105 21.47533001956781 27.03638915378076 27.714046397437286 23.774234429214143 106-107 21.631090674872386 26.815456571041295 27.542224622000834 24.011228132085485 108-109 21.629336087394424 26.888227723022993 27.48270520609578 23.999730983486796 110-111 21.648809698854954 26.95367426581668 27.630812227911328 23.76670380741704 112-113 21.611637029039475 27.016445581449812 27.77180683555644 23.60011055395428 114-115 21.439874344101206 27.29116283173454 27.532077736516374 23.736885087647877 116-117 21.335010742498632 27.173837863154997 27.676298154032995 23.814853240313372 118-119 21.253413578346944 27.148862577139777 27.53349727241174 24.06422657210154 120-121 21.132570729499324 27.321759373997427 27.567660165052292 23.978009731450953 122-123 21.28300805628204 27.34590430342887 27.537935825903038 23.83315181438605 124-125 21.276870983527054 27.325102364027032 27.444230265910395 23.953796386535515 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1230.0 1 984.5 2 639.0 3 437.0 4 299.0 5 251.5 6 219.5 7 191.5 8 197.5 9 190.5 10 165.0 11 164.5 12 175.0 13 187.0 14 206.0 15 225.5 16 242.0 17 273.0 18 328.5 19 419.5 20 586.0 21 797.5 22 1104.5 23 1640.5 24 2396.5 25 3599.5 26 5518.0 27 8208.5 28 11427.5 29 15568.5 30 20444.0 31 25907.0 32 33212.5 33 38959.0 34 42574.5 35 46949.5 36 53082.0 37 58804.0 38 62250.5 39 66038.0 40 68749.5 41 69963.0 42 69915.5 43 70074.5 44 70650.5 45 71990.0 46 71900.0 47 70233.5 48 68412.5 49 65705.5 50 64079.0 51 61324.0 52 58392.0 53 57733.5 54 58293.5 55 55229.5 56 49851.5 57 44161.5 58 39289.0 59 34076.5 60 26506.5 61 21286.5 62 17623.5 63 12919.5 64 8882.5 65 6593.0 66 4670.5 67 3485.5 68 2827.0 69 2100.0 70 1429.0 71 816.0 72 561.5 73 368.0 74 240.0 75 175.5 76 117.0 77 72.0 78 62.5 79 47.5 80 23.0 81 13.5 82 10.0 83 5.5 84 6.5 85 7.5 86 6.5 87 8.5 88 7.5 89 5.0 90 2.5 91 0.5 92 0.5 93 0.5 94 0.5 95 1.0 96 1.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08870669558573918 2 6.534563210735852E-4 3 5.4454693422798765E-5 4 0.002504915897448743 5 0.0015247314158383654 6 1.633640802683963E-4 7 0.0 8 0.0015247314158383654 9 7.07911014496384E-4 10-11 0.010264709710197566 12-13 0.0020965056967777525 14-15 0.004683103634360693 16-17 0.002613825284294341 18-19 0.013804264782679487 20-21 0.01728936516173861 22-23 0.010183027670063368 24-25 0.010890938684559754 26-27 0.010400846443754564 28-29 0.00841325013382241 30-31 0.018814096577576975 32-33 0.002368779163891746 34-35 0.0014430493757041673 36-37 9.80184481610378E-4 38-39 3.8118285395959134E-4 40-41 0.0020148236566435544 42-43 0.0030494628316767307 44-45 0.001143548561878774 46-47 0.00176977753624096 48-49 0.009202843188452992 50-51 0.00525487791530008 52-53 0.008549386867379405 54-55 0.009175615841741591 56-57 0.010564210524022961 58-59 0.008957797068050397 60-61 0.011217666845096546 62-63 0.01413099294321628 64-65 0.009447889308855584 66-67 0.014294357023484677 68-69 0.013804264782679487 70-71 0.005009831794897486 72-73 0.013858719476102286 74-75 0.014239902330061876 76-77 0.006371199130467455 78-79 0.008767205641070601 80-81 0.014294357023484677 82-83 0.018187867603214787 84-85 0.013967628862947883 86-87 0.017017091694624616 88-89 0.01674481822751062 90-91 0.012470124793820918 92-93 0.010564210524022961 94-95 0.008549386867379405 96-97 0.00972016277596958 98-99 0.0068885187179840435 100-101 0.011108757458250948 102-103 0.012905762341203306 104-105 0.01143548561878774 106-107 0.005908334236373666 108-109 0.003839055886307313 110-111 0.007133564838386638 112-113 0.009883526856237976 114-115 0.014348811716907476 116-117 0.012197851326706923 118-119 0.21787322838461784 120-121 0.026464981003480197 122-123 0.02681893651072839 124-125 0.23548932170689324 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1836389.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.802295072551075 #Duplication Level Percentage of deduplicated Percentage of total 1 84.07996500396712 53.64494736872878 2 9.055808547928805 11.555627381909678 3 2.9178604768034284 5.5849858536464065 4 1.3097063422385702 3.3424908202358723 5 0.688813688979499 2.1973947117141206 6 0.4414549174475066 1.6899502142528693 7 0.27529140903709987 1.2294956597226374 8 0.18373039630465648 0.937793676706115 9 0.13861792953473903 0.7959727838269361 >10 0.7796338943576936 9.541988693822448 >50 0.08109307317303172 3.5713644066941876 >100 0.04625293137215986 5.037870211691431 >500 0.0013777468877898163 0.5212672418774248 >1k 3.936419679399475E-4 0.3488509751711602 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.7227346711399383E-5 0.0 16-17 0.0 0.0 0.0 5.4454693422798765E-5 0.0 18-19 0.0 0.0 0.0 8.168204013419815E-5 0.0 20-21 0.0 0.0 0.0 1.0890938684559753E-4 0.0 22-23 0.0 0.0 0.0 1.0890938684559753E-4 0.0 24-25 0.0 0.0 0.0 4.900922408051889E-4 0.0 26-27 0.0 0.0 0.0 0.001361367335569969 0.0 28-29 0.0 0.0 0.0 0.004002419966575709 0.0 30-31 0.0 0.0 0.0 0.012851307647780509 0.0 32-33 0.0 0.0 0.0 0.036430189899852375 0.0 34-35 0.0 0.0 0.0 0.0657268149613181 0.0 36-37 0.0 0.0 0.0 0.11775827452680232 0.0 38-39 0.0 0.0 0.0 0.2021902766788518 0.0 40-41 0.0 0.0 0.0 0.298057764449689 0.0 42-43 0.0 0.0 0.0 0.3920737926441511 0.0 44-45 0.0 0.0 0.0 0.5078172435143099 0.0 46-47 0.0 0.0 0.0 0.6428376558561395 0.0 48-49 0.0 0.0 0.0 0.7924791533819904 0.0 50-51 0.0 0.0 0.0 0.9563605532379034 0.0 52-53 0.0 0.0 0.0 1.1142519368173083 0.0 54-55 0.0 0.0 0.0 1.284395626416843 0.0 56-57 0.0 0.0 0.0 1.4868037218693861 0.0 58-59 0.0 0.0 0.0 1.7103402383699748 0.0 60-61 0.0 0.0 0.0 1.953861627356731 0.0 62-63 0.0 0.0 0.0 2.190685089052483 0.0 64-65 0.0 0.0 0.0 2.434342614772796 0.0 66-67 0.0 0.0 0.0 2.717942658118732 0.0 68-69 0.0 0.0 0.0 3.0368837974960643 0.0 70-71 0.0 0.0 0.0 3.387054703551372 0.0 72-73 0.0 0.0 0.0 3.7415547577337915 0.0 74-75 0.0 0.0 0.0 4.089520248705476 0.0 76-77 0.0 0.0 0.0 4.470893694092046 0.0 78-79 0.0 0.0 0.0 4.899343221942628 0.0 80-81 0.0 0.0 0.0 5.365121442134537 5.4454693422798765E-5 82-83 0.0 0.0 0.0 5.852218674801472 5.4454693422798765E-5 84-85 0.0 0.0 0.0 6.33645703606371 5.4454693422798765E-5 86-87 0.0 0.0 0.0 6.841633226947014 5.4454693422798765E-5 88-89 0.0 0.0 0.0 7.383702472624265 5.4454693422798765E-5 90-91 0.0 0.0 0.0 7.973827985247135 5.4454693422798765E-5 92-93 0.0 0.0 0.0 8.596707995963818 5.4454693422798765E-5 94-95 0.0 0.0 0.0 9.226966617639292 5.4454693422798765E-5 96-97 0.0 0.0 0.0 9.86550235271503 5.4454693422798765E-5 98-99 0.0 0.0 0.0 10.52159972641962 5.4454693422798765E-5 100-101 0.0 0.0 0.0 11.243042732231569 5.4454693422798765E-5 102-103 0.0 0.0 0.0 12.000153562235452 5.4454693422798765E-5 104-105 0.0 0.0 0.0 12.758380713454503 5.4454693422798765E-5 106-107 0.0 0.0 0.0 13.51462026836362 5.4454693422798765E-5 108-109 0.0 0.0 0.0 14.30048317649474 5.4454693422798765E-5 110-111 0.0 0.0 0.0 15.123538640233633 5.4454693422798765E-5 112-113 0.0 0.0 0.0 15.997999334563646 5.4454693422798765E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1700 0.0 55.324596 1 GTATCAA 2895 0.0 48.32027 1 ATCAACG 3135 0.0 42.87483 3 TCAACGC 3190 0.0 42.325504 4 CAACGCA 3225 0.0 41.86958 5 GTACATG 9510 0.0 40.560616 1 TACATGG 9900 0.0 38.08777 2 TATCAAC 3680 0.0 36.848392 2 AACGCAG 3660 0.0 36.729748 6 ACATGGG 9850 0.0 36.59046 3 CATGGGA 7445 0.0 35.63175 4 CGCAGAG 4445 0.0 30.24317 8 ACGCAGA 4475 0.0 29.9075 7 ATGGGAT 3340 0.0 27.248804 5 GAGTACA 6490 0.0 26.415442 1 ATGGGAG 3365 0.0 25.455399 5 CATGGGG 2740 0.0 23.661535 4 GCGTTAG 155 2.1005953E-4 23.042501 1 AGTACAT 7145 0.0 22.807613 2 TAGCCCT 1555 0.0 22.567766 4 >>END_MODULE