FastQCFastQC Report
Thu 26 May 2016
SRR1364438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364438_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836389
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29310.1596067064222232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24310.13237935971082382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26350.073.030681
GTATCAA50550.055.2758031
ATCAACG62550.044.269823
CAACGCA63650.043.4715775
TCAACGC63850.043.460564
TATCAAC66500.041.7320822
AACGCAG68000.040.7748536
GTACATG92950.035.6376271
TACATGG95700.034.350492
ACGCAGA80850.034.2942477
CGCAGAG81100.034.1857388
ACATGGG94150.034.0286033
CATGGGA72350.031.934754
GTCTACG953.2620304E-431.3566591
ATGGGAT33050.028.4469995
GCAGAGT98150.027.4591929
GGACCGA3950.027.1138276
GAGTACA65300.026.4586831
TAAGGTG10650.025.7015365
TGGTATC13950.025.614322
Produced by FastQC (version 0.11.5)