FastQCFastQC Report
Thu 26 May 2016
SRR1364437_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364437_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735737
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.044.4268261
GTATCAA22400.042.250931
GTACATG60400.039.9121631
ATCAACG23250.037.863923
CAACGCA24000.037.4263535
TACATGG64450.036.4553262
ACATGGG63150.035.698723
TCAACGC25350.035.196554
AACGCAG26250.033.991776
TATCAAC27850.032.4642332
ACGCAGA29800.029.543187
CGCAGAG31150.028.262828
CATGGGG34850.027.6502484
CATGGGA37650.027.1737944
GAGTACA48950.022.2528461
AGTACAT47100.020.7114142
GGACCGA2659.266223E-720.2026546
GCAGAGT42100.020.0640249
GGTAAGG10450.019.3664381
AGAGTAC36100.019.36165410-11
Produced by FastQC (version 0.11.5)