FastQCFastQC Report
Thu 26 May 2016
SRR1364437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364437_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735737
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20740.1194881482620927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19310.11124957294797541No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22100.063.6209221
GTATCAA39250.053.1261861
ATCAACG48100.042.711163
CAACGCA50800.040.176525
TCAACGC51000.040.048964
TATCAAC52600.038.947322
AACGCAG53300.038.5131236
GTACATG64800.032.7306941
ACGCAGA63700.032.131867
TACATGG66650.031.4519392
CGCAGAG65750.031.1282378
ACATGGG66900.030.708623
GCAGAGT78300.025.6830629
CATGGGA42050.025.0653974
CATGGGG33000.024.90194
AGAGTAC70050.023.35696410-11
GTGGTAT15050.023.3558541
CAGAGTA73500.023.11056710-11
GAGTACT45650.022.22160512-13
AGTACTT49600.021.4715412-13
Produced by FastQC (version 0.11.5)